##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935315.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1108809
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.223898795915254	33.0	31.0	34.0	30.0	34.0
2	32.37659777292573	34.0	31.0	34.0	30.0	34.0
3	32.432710232330365	34.0	31.0	34.0	30.0	34.0
4	35.93170690353343	37.0	35.0	37.0	35.0	37.0
5	31.82263221167938	37.0	35.0	37.0	0.0	37.0
6	33.53729993172855	37.0	35.0	37.0	17.0	37.0
7	35.26270980845213	37.0	35.0	37.0	32.0	37.0
8	35.79417194485254	37.0	35.0	37.0	35.0	37.0
9	37.85778704898679	39.0	38.0	39.0	35.0	39.0
10	37.47850711889965	39.0	37.0	39.0	35.0	39.0
11	37.3848976694814	39.0	37.0	39.0	35.0	39.0
12	37.21065936513863	39.0	37.0	39.0	34.0	39.0
13	37.14650855106696	39.0	37.0	39.0	33.0	39.0
14	38.22662965398008	40.0	38.0	41.0	33.0	41.0
15	38.32630597334617	40.0	38.0	41.0	34.0	41.0
16	38.4121007315056	40.0	38.0	41.0	34.0	41.0
17	38.44437139308934	40.0	38.0	41.0	34.0	41.0
18	38.44459325276039	40.0	38.0	41.0	34.0	41.0
19	38.46457956239533	40.0	38.0	41.0	34.0	41.0
20	38.36773240476944	40.0	37.0	41.0	34.0	41.0
21	38.32308269503584	40.0	37.0	41.0	34.0	41.0
22	38.30911455444536	40.0	37.0	41.0	34.0	41.0
23	38.27595555230883	40.0	37.0	41.0	34.0	41.0
24	38.23769287586951	40.0	37.0	41.0	34.0	41.0
25	38.13355140515635	40.0	37.0	41.0	34.0	41.0
26	38.04663562435009	40.0	37.0	41.0	34.0	41.0
27	37.99221326666721	40.0	37.0	41.0	34.0	41.0
28	38.0138797574695	40.0	37.0	41.0	34.0	41.0
29	37.966854525892195	40.0	37.0	41.0	34.0	41.0
30	37.90365067383111	40.0	36.0	41.0	34.0	41.0
31	37.70467321242883	40.0	36.0	41.0	33.0	41.0
32	37.72812269741678	40.0	36.0	41.0	33.0	41.0
33	37.651591031458075	39.0	36.0	41.0	33.0	41.0
34	37.68215716142275	39.0	36.0	41.0	33.0	41.0
35	37.56460129742814	39.0	36.0	41.0	33.0	41.0
36	37.44663327949178	39.0	35.0	41.0	33.0	41.0
37	37.49054165325137	39.0	36.0	41.0	33.0	41.0
38	37.58146263242813	39.0	36.0	41.0	33.0	41.0
39	37.582770341871324	39.0	36.0	41.0	33.0	41.0
40	37.57421160903276	39.0	36.0	41.0	33.0	41.0
41	37.465870136335475	39.0	36.0	41.0	33.0	41.0
42	37.32497210971412	39.0	35.0	41.0	32.0	41.0
43	37.257768470494014	39.0	35.0	41.0	32.0	41.0
44	37.32090648614865	39.0	35.0	41.0	33.0	41.0
45	37.38568860822738	39.0	35.0	41.0	33.0	41.0
46	37.29205751396318	39.0	35.0	41.0	33.0	41.0
47	37.22585675260572	39.0	35.0	41.0	32.0	41.0
48	37.19258682063367	39.0	35.0	41.0	32.0	41.0
49	37.19363479192539	39.0	35.0	41.0	32.0	41.0
50	37.15506638203694	39.0	35.0	41.0	32.0	41.0
51	36.58217961795043	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	4.0
11	11.0
12	7.0
13	7.0
14	9.0
15	12.0
16	23.0
17	45.0
18	95.0
19	138.0
20	269.0
21	485.0
22	771.0
23	1285.0
24	1926.0
25	2875.0
26	4177.0
27	5429.0
28	7159.0
29	9791.0
30	13279.0
31	18592.0
32	26251.0
33	39406.0
34	63363.0
35	92555.0
36	98486.0
37	155021.0
38	258332.0
39	308763.0
40	238.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.99026162305681	4.447745283452786	58.79560862150289	14.766384471987513
2	17.915619371776383	3.93539374229466	56.45345591531093	21.695530970618023
3	18.854284191416195	3.712992950093298	55.567730781406	21.864992077084512
4	18.54359046508461	3.8478223030296475	53.596065688500005	24.01252154338574
5	15.14787488196795	15.99202387426509	47.71552178959587	21.144579454171097
6	19.18806575343454	5.384786739645872	55.78463017526012	19.642517331659466
7	79.81717320115548	1.542736395537915	12.134912324845848	6.505178078460763
8	82.55867331524185	1.662865290595585	8.668129497505884	7.110331896656683
9	77.85028801173151	3.7507812436587367	11.720864459072752	6.678066285537004
10	35.205161574265716	25.767287242437607	20.878438035766305	18.149113147530368
11	24.542188961308938	28.939339417338783	26.399046183788187	20.11942543756409
12	22.15142553857337	23.918366463475678	33.67126349082664	20.258944507124312
13	20.974216479123093	26.17637483101237	34.26920236037046	18.58020632949408
14	17.426355666305017	27.807494347538665	33.62355464286455	21.142595343291767
15	14.339439885498765	26.40436720841912	38.22515870632363	21.03103419975848
16	15.774312798687602	26.510246579888875	33.27723710756316	24.43820351386037
17	16.4103105223713	25.84241289527773	30.770944319535648	26.976332262815326
18	17.56010277694355	24.638418338956484	34.38346910964828	23.418009774451686
19	19.486854814490144	26.89561502476982	31.373663092561475	22.24386706817856
20	20.627538196389096	28.67770734184156	30.421921178489715	20.272833283279628
21	20.097149283600693	28.579133105882075	31.136201095048833	20.1875165154684
22	19.04223360380372	26.174480906991192	28.721538154903143	26.061747334301938
23	17.82209559987338	26.528283951519153	28.56073498681919	27.08888546178828
24	19.677960766912967	25.92222826474172	29.95258876866981	24.447222199675508
25	16.703598185079667	28.97324967600371	28.149212353074333	26.173939785842286
26	15.79153848859452	30.59976966276428	29.553241360775388	24.05545048786581
27	17.76311339464236	30.7422648986435	29.420396118718372	22.07422558799577
28	15.078701561765822	28.257256209139715	33.446427653455196	23.217614575639267
29	18.62187265796003	26.80109919742715	30.688874278617867	23.888153865994955
30	22.970953518595177	28.898214210021745	27.93222277236206	20.19860949902102
31	23.246474370247718	29.91858832314673	26.48066529041521	20.354272016190343
32	24.526225887416135	29.461431139177265	25.352968816090055	20.65937415731654
33	23.729334808790334	27.032608862301803	27.425282442693018	21.81277388621485
34	21.536531539697098	25.83303346202998	29.29702049676725	23.33341450150567
35	21.787701939648755	27.18150736510977	26.969928995886576	24.060861699354895
36	22.609664964840654	28.356642126822564	29.617003469488434	19.41668943884835
37	21.25577985027178	27.650839774929675	32.05258976072525	19.0407906140733
38	23.20958794526379	27.11197329747504	28.70395171756362	20.974487039697546
39	25.586101844411434	23.375351390546072	30.12313211743411	20.91541464760838
40	27.787563051887204	23.331610764342642	27.940429776453833	20.94039640731632
41	23.38220559176558	25.2884852125118	26.338079867677845	24.991229328044774
42	21.705000590723923	25.596383146240697	29.715307144873464	22.98330911816192
43	21.713838902822758	24.940544313763688	31.106800179291472	22.23881660412208
44	21.290772351234523	24.278482588074233	28.890097392788118	25.540647667903126
45	23.417919587593534	24.648068332778685	26.172496796111865	25.76151528351592
46	25.372178616876305	25.129846529023485	28.822276875458265	20.67569797864195
47	20.881865136376057	24.96922373465583	32.674608521395484	21.47430260757263
48	21.359675110862195	23.174685631159196	32.23945693081496	23.22618232716365
49	24.34594235797148	20.374383685558108	33.77046903479319	21.50920492167722
50	24.51792869646621	20.374564059274412	29.932116351869436	25.17539089238994
51	22.654307459625596	20.678764331819096	26.988417301807615	29.67851090674769
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	172.0
1	345.5
2	519.0
3	1050.5
4	1582.0
5	1140.0
6	698.0
7	938.5
8	1179.0
9	1581.0
10	1983.0
11	2512.0
12	3041.0
13	3279.5
14	3518.0
15	3901.0
16	4284.0
17	3781.0
18	3278.0
19	3673.0
20	4068.0
21	3958.0
22	3848.0
23	4085.5
24	4323.0
25	5753.0
26	8161.0
27	9139.0
28	11080.5
29	13022.0
30	15516.5
31	18011.0
32	20275.0
33	22539.0
34	25817.0
35	29095.0
36	32897.0
37	36699.0
38	40080.0
39	43461.0
40	52299.0
41	61137.0
42	70422.0
43	79707.0
44	92589.5
45	105472.0
46	124644.0
47	143816.0
48	156632.0
49	169448.0
50	153444.5
51	137441.0
52	106015.5
53	74590.0
54	60280.5
55	45971.0
56	36138.5
57	26306.0
58	21947.0
59	17588.0
60	14902.5
61	12217.0
62	10144.5
63	8072.0
64	7133.0
65	6194.0
66	4964.5
67	3735.0
68	2750.5
69	1766.0
70	1570.5
71	1375.0
72	1244.5
73	1114.0
74	906.5
75	465.0
76	231.0
77	195.0
78	159.0
79	111.5
80	64.0
81	51.0
82	38.0
83	29.0
84	20.0
85	12.5
86	5.0
87	3.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1108809.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.528956578583024
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.86212650070278	16.9419558095312
2	13.724030214928229	7.281684033099848
3	6.259189483746416	4.981492980942965
4	3.7292582102667735	3.9573331652196586
5	2.4096692477456094	3.1963005421095043
6	1.6601498686178957	2.642522626710268
7	1.234397837400235	2.2923100640361334
8	0.8912355598528546	1.8914839574900408
9	0.68918534618754	1.6455031311244463
>10	4.467625366347381	24.05826461003195
>50	0.879008370520595	16.040533516455284
>100	0.17607386795538196	8.029427805841143
>500	0.012601031168819414	2.297581315628492
>1k	0.004086820919617107	1.7654242590397453
>5k	0.001362273639872369	2.9781821827393617
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC	9453	0.8525363701052211	No Hit
CTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGCT	8897	0.8023924769730404	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC	8597	0.7753364195276192	No Hit
GCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC	6016	0.5425641386388458	No Hit
TCCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTG	3232	0.2914839255453374	No Hit
TTCCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCT	2245	0.20246949654990176	No Hit
GCCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTG	1712	0.15439990115520347	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCC	1622	0.1462830839215771	No Hit
TTTCCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTC	1610	0.14520084162376026	No Hit
TGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGCTT	1523	0.13735458496458813	Illumina Single End Adapter 1 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTG	1461	0.13176299975920108	No Hit
TTCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTG	1434	0.12932795458911317	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTC	1303	0.11751347617127926	No Hit
CGCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTG	1171	0.10560881089529395	No Hit
TCTGACTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC	1133	0.10218171028554063	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8278251709717364	0.0
2	0.0	0.0	0.0	3.143553127725334	0.0
3	0.0	0.0	0.0	4.339250493096647	0.0
4	0.0	0.0	0.0	5.736605673294499	0.0
5	0.0	0.0	0.0	8.050890640317673	0.0
6	0.0	0.0	0.0	9.571260695034042	0.0
7	0.0	0.0	0.0	10.91513506834811	0.0
8	0.0	0.0	0.0	13.06257434779119	0.0
9	0.0	0.0	0.0	14.038756900421983	0.0
10	0.0	0.0	0.0	16.023048153469173	0.0
11	0.0	0.0	0.0	19.48721556192275	0.0
12	0.0	0.0	0.0	22.25045070882361	0.0
13	0.0	0.0	0.0	23.144202473104023	0.0
14	0.0	0.0	0.0	23.41620603728866	0.0
15	0.0	0.0	0.0	23.820694096097704	0.0
16	0.0	0.0	0.0	24.99727184754092	0.0
17	0.0	0.0	0.0	26.814987973582465	0.0
18	0.0	0.0	0.0	28.9337478321334	0.0
19	0.0	0.0	0.0	30.256969414930794	0.0
20	0.0	0.0	0.0	31.595342389897628	0.0
21	0.0	0.0	0.0	33.45797157129857	0.0
22	9.01868581514039E-5	0.0	0.0	35.21715642639986	0.0
23	9.01868581514039E-5	0.0	0.0	36.95253195094917	0.0
24	9.01868581514039E-5	0.0	0.0	38.14741763459712	0.0
25	9.01868581514039E-5	0.0	0.0	39.230742174711786	0.0
26	9.01868581514039E-5	0.0	0.0	40.12196870696396	0.0
27	9.01868581514039E-5	0.0	0.0	41.049269982476694	0.0
28	9.01868581514039E-5	0.0	0.0	41.93463436894903	0.0
29	9.01868581514039E-5	0.0	0.0	42.93192064638725	0.0
30	9.01868581514039E-5	0.0	0.0	43.985754083886405	0.0
31	9.01868581514039E-5	0.0	0.0	44.98249924017572	0.0
32	9.01868581514039E-5	0.0	0.0	45.88545006398758	0.0
33	9.01868581514039E-5	0.0	0.0	46.71111075036368	0.0
34	9.01868581514039E-5	0.0	0.0	47.55002890488804	0.0
35	9.01868581514039E-5	0.0	0.0	48.435844225651124	0.0
36	9.01868581514039E-5	0.0	0.0	49.24418903526216	0.0
37	9.01868581514039E-5	0.0	0.0	50.0114988244143	0.0
38	9.01868581514039E-5	0.0	0.0	50.77348758893552	0.0
39	9.01868581514039E-5	0.0	0.0	51.63901086661454	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGA	35	1.2118835E-7	45.000004	8
ACCCTCG	45	3.8562575E-10	45.000004	31
AACGGTA	35	1.2118835E-7	45.000004	16
GGAACCG	45	3.8562575E-10	45.000004	8
ACTACGG	35	1.2118835E-7	45.000004	2
GTCAATC	35	1.2118835E-7	45.000004	17
TAAGGGT	45	3.8562575E-10	45.000004	4
TGCTACG	35	1.2118835E-7	45.000004	1
GTACGAC	35	1.2118835E-7	45.000004	9
TGGTACG	35	1.2118835E-7	45.000004	1
ACGGTAA	35	1.2118835E-7	45.000004	17
TCCGGAT	40	6.8139343E-9	45.0	25
TCGCCCA	20	7.0331787E-4	45.0	15
AGATCCG	20	7.0331787E-4	45.0	28
ACCGGAT	20	7.0331787E-4	45.0	32
GTCGACT	20	7.0331787E-4	45.0	22
GATCGAT	25	3.890717E-5	45.0	9
CGAAACC	40	6.8139343E-9	45.0	22
CCCTATT	20	7.0331787E-4	45.0	14
GACCGTT	25	3.890717E-5	45.0	9
>>END_MODULE