SRR2935314.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935314.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1547249
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC	8065	0.521247711260437	No Hit
CTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGCT	7699	0.4975928244258035	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC	7047	0.4554535178242157	No Hit
GCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC	6174	0.3990307959481635	No Hit
TCCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTG	3109	0.2009372764176936	No Hit
GCCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTG	1823	0.11782201830474605	No Hit
TTCCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCT	1768	0.11426732219571639	No Hit
TGCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTG	1616	0.10444343476712539	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGAAA	20	7.034044E-4	45.000004	19
ACGCTAG	20	7.034044E-4	45.000004	13
CGACTTA	20	7.034044E-4	45.000004	34
GTGCGAT	20	7.034044E-4	45.000004	24
TAATGCG	20	7.034044E-4	45.000004	24
CCTTACG	25	3.891434E-5	45.0	1
TTATCCG	25	3.891434E-5	45.0	1
CGTAGAC	25	3.891434E-5	45.0	12
TATCCGG	35	1.2123019E-7	45.0	2
ATAAGCG	220	0.0	43.977276	10
TTACGGG	1145	0.0	43.03493	3
TCTTACG	175	0.0	42.428574	1
CTTACGG	345	0.0	42.3913	2
TACGGGA	765	0.0	42.352943	4
GCTCGAT	80	0.0	42.187504	27
GCTTACG	135	0.0	41.666668	1
GTTTACG	130	0.0	41.53846	1
TCGCCTA	60	3.6379788E-12	41.250004	22
TATGGGA	2255	0.0	40.410202	4
TAAGCGT	195	0.0	40.384617	11