##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2935314.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1547249 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.289688989942796 33.0 31.0 34.0 30.0 34.0 2 32.43520661509557 34.0 31.0 34.0 30.0 34.0 3 32.49687542212016 34.0 31.0 34.0 30.0 34.0 4 35.95330163406149 37.0 35.0 37.0 35.0 37.0 5 31.794864950631734 37.0 35.0 37.0 0.0 37.0 6 33.477133286238995 37.0 35.0 37.0 17.0 37.0 7 35.21818660086386 37.0 35.0 37.0 32.0 37.0 8 35.7570804699179 37.0 35.0 37.0 35.0 37.0 9 37.76903523608676 39.0 38.0 39.0 35.0 39.0 10 37.505056393638 39.0 37.0 39.0 35.0 39.0 11 37.43421970219402 39.0 37.0 39.0 35.0 39.0 12 37.28812233842129 39.0 37.0 39.0 34.0 39.0 13 37.25187736427686 39.0 37.0 39.0 34.0 39.0 14 38.46766809996323 40.0 38.0 41.0 34.0 41.0 15 38.53856554439525 40.0 38.0 41.0 34.0 41.0 16 38.57923385311608 40.0 38.0 41.0 35.0 41.0 17 38.55548589787423 40.0 38.0 41.0 34.0 41.0 18 38.53073228678771 40.0 38.0 41.0 34.0 41.0 19 38.584093445851316 40.0 38.0 41.0 34.0 41.0 20 38.51039296196023 40.0 38.0 41.0 34.0 41.0 21 38.43490672800564 40.0 38.0 41.0 34.0 41.0 22 38.41121758682669 40.0 38.0 41.0 34.0 41.0 23 38.372927692956985 40.0 37.0 41.0 34.0 41.0 24 38.369453139087504 40.0 37.0 41.0 34.0 41.0 25 38.27512378421314 40.0 37.0 41.0 34.0 41.0 26 38.254240267726786 40.0 37.0 41.0 34.0 41.0 27 38.179598112521 40.0 37.0 41.0 34.0 41.0 28 38.11831321267618 40.0 37.0 41.0 34.0 41.0 29 37.96089705018391 40.0 37.0 41.0 34.0 41.0 30 37.93622099610341 40.0 37.0 41.0 33.0 41.0 31 37.75918226478091 40.0 36.0 41.0 33.0 41.0 32 37.77508597517271 40.0 36.0 41.0 33.0 41.0 33 37.76416400980062 40.0 36.0 41.0 33.0 41.0 34 37.73712311334504 40.0 36.0 41.0 33.0 41.0 35 37.656512946526384 40.0 36.0 41.0 33.0 41.0 36 37.66648354595802 40.0 36.0 41.0 33.0 41.0 37 37.61031546958505 40.0 36.0 41.0 33.0 41.0 38 37.52553855261823 39.0 36.0 41.0 33.0 41.0 39 37.479376622637986 39.0 36.0 41.0 33.0 41.0 40 37.498052995994826 39.0 36.0 41.0 33.0 41.0 41 37.462936476287915 39.0 36.0 41.0 33.0 41.0 42 37.42844041262912 39.0 35.0 41.0 33.0 41.0 43 37.3153261045895 39.0 35.0 41.0 33.0 41.0 44 37.23925173000597 39.0 35.0 41.0 32.0 41.0 45 37.19095051927647 39.0 35.0 41.0 32.0 41.0 46 37.110586595951915 39.0 35.0 41.0 32.0 41.0 47 37.060625018985306 39.0 35.0 41.0 32.0 41.0 48 36.997335270535 39.0 35.0 41.0 32.0 41.0 49 36.939896551880146 39.0 35.0 41.0 32.0 41.0 50 36.83619960329592 39.0 35.0 41.0 32.0 41.0 51 36.18872851105414 38.0 35.0 40.0 31.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 17.0 10 18.0 11 16.0 12 27.0 13 17.0 14 33.0 15 31.0 16 61.0 17 88.0 18 164.0 19 264.0 20 486.0 21 860.0 22 1324.0 23 2143.0 24 3277.0 25 4889.0 26 6746.0 27 8833.0 28 11073.0 29 14446.0 30 19089.0 31 25857.0 32 35374.0 33 51839.0 34 84503.0 35 121583.0 36 133423.0 37 210085.0 38 341200.0 39 469036.0 40 445.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.674437016924877 4.8829244678781505 55.71798721472756 16.724651300469414 2 18.985825810842343 4.622462189343796 53.315594322568636 23.07611767724523 3 20.373062125100745 4.8393632828329505 52.76261286968031 22.02496172238599 4 19.417882965185306 4.597191531550513 50.59657495335269 25.388350549911486 5 16.276630329054985 16.90361409184947 43.83470275307982 22.98505282601572 6 20.477376298191178 6.491198249279851 53.76154710715599 19.26987834537298 7 82.10953763744556 2.3871400143092676 9.65940194500045 5.843920403244727 8 83.593914101738 2.784490408460435 7.615710205661791 6.0058852841397865 9 77.71974646614733 5.545067406732853 10.352115270392808 6.383070856727004 10 37.0436820447129 30.66403662241824 16.745397799578477 15.546883533290377 11 26.54343289283108 24.898125641057128 27.872759975931476 20.685681490180315 12 22.50032153842077 21.97299852835581 33.10979680710732 22.416883126116094 13 22.28012427217597 24.2823230132965 33.779049138180085 19.65850357634744 14 19.16026444353818 27.292504309261144 31.63026765569084 21.916963591509834 15 16.811385885529738 24.679931930801054 36.26384634923015 22.24483583443906 16 19.72895119014457 25.22897090255027 31.605772567957715 23.43630533934745 17 19.45575663645606 24.394069732796726 29.33083168901709 26.81934194173013 18 20.468198719146045 22.630423416011254 32.585188292252894 24.316189572589803 19 22.25511213773607 24.485910154086383 30.113898926417143 23.145078781760404 20 24.107884380600666 25.760688809622756 29.365667710885578 20.765759098891 21 24.22169928692796 26.53289806618069 29.109212544328678 20.136190102562676 22 22.815655398710874 24.351607272003406 27.928988805292494 24.90374852399323 23 20.70332570904877 24.888366384466885 28.10045441942441 26.307853487059933 24 21.944754851998614 23.848843980509923 29.059899214670683 25.14650195282078 25 20.337902949040522 26.334998439165254 27.18405376251657 26.14304484927765 26 19.809222691370294 26.896898947745324 26.894895391756595 26.398982969127786 27 22.4546921665485 25.996720631262328 27.236275479900137 24.312311722289042 28 20.747210048285698 24.289884821382984 30.157718634815726 24.80518649551559 29 20.653236809330625 22.992937788293933 30.78929118713278 25.564534215242666 30 23.428420377069237 23.345886796501404 30.065425797657646 23.16026702877171 31 23.83973103230314 25.476636275092112 27.422735448528325 23.26089724407642 32 23.275245290189233 25.253724513636783 28.153904122736545 23.317126073437436 33 23.391645430050367 23.638341340017025 29.766572801145774 23.203440428786834 34 21.73955194025008 22.999077717936803 30.90084401411796 24.360526327695155 35 22.16159131464942 22.11609120445384 30.03698822878541 25.685329252111327 36 24.04040978536745 23.101000550008436 29.77361756252549 23.08497210209863 37 23.778202474197755 23.87036604967914 30.603606788564736 21.74782468755837 38 22.796589301398807 24.889271216203728 29.679385800217027 22.63475368218044 39 21.572222699772308 22.592097328872082 33.429654826081645 22.406025145273965 40 22.855467995132006 22.167408090100558 32.31541917299672 22.661704741770716 41 21.37303045598995 22.705912235199378 29.63763427864552 26.283423030165153 42 24.148278654566912 22.205087868856275 28.90368647838842 24.742946998188398 43 24.004992085953845 22.102518728401183 29.712864574480253 24.17962461116472 44 22.173483388905083 22.105039331096677 29.867784693995603 25.85369258600264 45 22.12720770864935 21.61765817912954 29.33516195518627 26.919972157034838 46 23.527693344768682 21.960524776555033 31.032690924343786 23.479090954332495 47 20.94045625494022 22.48694295488315 32.8149509225729 23.757649867603728 48 21.276665876016075 21.468167050035255 32.441966354478176 24.813200719470494 49 23.105136923662577 20.0454484055249 33.93713616877439 22.912278502038134 50 22.321681901232445 20.553382164086067 31.691343797927807 25.433592136753685 51 21.674468686035667 20.28710311010057 29.524336419024994 28.51409178483877 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 284.0 1 693.5 2 1103.0 3 1856.5 4 2610.0 5 1922.5 6 1235.0 7 1406.5 8 1578.0 9 1907.5 10 2237.0 11 2283.5 12 2330.0 13 2426.0 14 2522.0 15 2528.5 16 2535.0 17 2736.0 18 2937.0 19 3724.5 20 4512.0 21 4650.0 22 4788.0 23 6134.0 24 7480.0 25 7868.5 26 11361.5 27 14466.0 28 16630.0 29 18794.0 30 21005.5 31 23217.0 32 26950.5 33 30684.0 34 34572.5 35 38461.0 36 42030.0 37 45599.0 38 49532.0 39 53465.0 40 59032.5 41 64600.0 42 76043.5 43 87487.0 44 99550.5 45 111614.0 46 133692.0 47 155770.0 48 176418.0 49 197066.0 50 186854.0 51 176642.0 52 148052.5 53 119463.0 54 104123.0 55 88783.0 56 80200.5 57 71618.0 58 63366.0 59 55114.0 60 48639.5 61 42165.0 62 38293.5 63 34422.0 64 30268.5 65 26115.0 66 21585.5 67 17056.0 68 13541.5 69 10027.0 70 9180.5 71 8334.0 72 6860.0 73 5386.0 74 4345.0 75 2404.5 76 1505.0 77 1157.0 78 809.0 79 709.0 80 609.0 81 404.5 82 200.0 83 117.0 84 34.0 85 25.0 86 16.0 87 15.0 88 14.0 89 8.0 90 2.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1547249.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.534072851395813 #Duplication Level Percentage of deduplicated Percentage of total 1 67.35264658414111 17.197873846137746 2 12.657318538092815 6.463857873099693 3 5.751343675931675 4.405656852439619 4 3.205584173484951 3.274064792681846 5 1.9735304922034482 2.519613568118694 6 1.3069004242538167 2.0022294385450228 7 0.8907086806807303 1.5920394239310691 8 0.6581240762315855 1.3443670486203898 9 0.5328495686874473 1.2245237735130103 >10 4.079732180018698 23.89357884557346 >50 1.4181889684512932 24.679934140167155 >100 0.16085880320713067 6.8994700580576955 >500 0.0077960646481257 1.336856083380846 >1k 0.0033782946808544705 1.2426318495792477 >5k 0.00103947528641676 1.923302406154456 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC 8065 0.521247711260437 No Hit CTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGCT 7699 0.4975928244258035 Illumina Single End Adapter 1 (95% over 21bp) TCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC 7047 0.4554535178242157 No Hit GCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC 6174 0.3990307959481635 No Hit TCCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTG 3109 0.2009372764176936 No Hit GCCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTG 1823 0.11782201830474605 No Hit TTCCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCT 1768 0.11426732219571639 No Hit TGCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTG 1616 0.10444343476712539 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 6.463083834599344E-5 0.0 0.0 0.5145907349107998 0.0 2 6.463083834599344E-5 0.0 0.0 1.9809351953046987 0.0 3 6.463083834599344E-5 0.0 0.0 2.8034918749341573 0.0 4 6.463083834599344E-5 0.0 0.0 3.712266092917171 0.0 5 6.463083834599344E-5 0.0 0.0 5.270063189570651 0.0 6 6.463083834599344E-5 0.0 0.0 6.336536653117888 0.0 7 6.463083834599344E-5 0.0 0.0 7.281051724706237 0.0 8 6.463083834599344E-5 0.0 0.0 8.762132016243022 0.0 9 6.463083834599344E-5 0.0 0.0 9.484640158112883 0.0 10 6.463083834599344E-5 0.0 0.0 10.793026849589173 0.0 11 6.463083834599344E-5 0.0 0.0 13.049289416247804 0.0 12 6.463083834599344E-5 0.0 0.0 15.027316223826935 0.0 13 1.2926167669198688E-4 0.0 0.0 15.69876600340346 0.0 14 1.2926167669198688E-4 0.0 0.0 15.946496006783653 0.0 15 1.2926167669198688E-4 0.0 0.0 16.30933353325806 0.0 16 1.2926167669198688E-4 0.0 0.0 17.18928239733876 0.0 17 1.2926167669198688E-4 0.0 0.0 18.43090543280364 0.0 18 1.2926167669198688E-4 0.0 0.0 19.874887623129826 0.0 19 1.2926167669198688E-4 0.0 0.0 20.76110567853009 0.0 20 1.2926167669198688E-4 0.0 0.0 21.64454460788147 0.0 21 1.2926167669198688E-4 0.0 0.0 22.791224941816086 0.0 22 1.2926167669198688E-4 0.0 0.0 23.894990399088964 0.0 23 1.2926167669198688E-4 0.0 0.0 25.033204093200254 0.0 24 1.2926167669198688E-4 0.0 0.0 25.88387518751022 0.0 25 1.2926167669198688E-4 0.0 0.0 26.64231807550045 0.0 26 1.2926167669198688E-4 0.0 0.0 27.339361667061993 0.0 27 1.9389251503798032E-4 0.0 0.0 27.98741508315727 0.0 28 2.5852335338397375E-4 0.0 0.0 28.66422922231651 0.0 29 2.5852335338397375E-4 0.0 0.0 29.41019835850597 0.0 30 2.5852335338397375E-4 0.0 0.0 30.21433524920682 0.0 31 2.5852335338397375E-4 0.0 0.0 30.949834189584223 0.0 32 2.5852335338397375E-4 0.0 0.0 31.607259077239668 0.0 33 2.5852335338397375E-4 0.0 0.0 32.268044768489105 0.0 34 2.5852335338397375E-4 0.0 0.0 32.93148032411073 0.0 35 2.5852335338397375E-4 0.0 0.0 33.61585627135645 0.0 36 3.8778503007596063E-4 0.0 0.0 34.272247065598364 0.0 37 3.8778503007596063E-4 0.0 0.0 34.95229274667491 0.0 38 3.8778503007596063E-4 0.0 0.0 35.60965300349201 0.0 39 4.5241586842195404E-4 0.0 0.0 36.29738975433172 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACGAAA 20 7.034044E-4 45.000004 19 ACGCTAG 20 7.034044E-4 45.000004 13 CGACTTA 20 7.034044E-4 45.000004 34 GTGCGAT 20 7.034044E-4 45.000004 24 TAATGCG 20 7.034044E-4 45.000004 24 CCTTACG 25 3.891434E-5 45.0 1 TTATCCG 25 3.891434E-5 45.0 1 CGTAGAC 25 3.891434E-5 45.0 12 TATCCGG 35 1.2123019E-7 45.0 2 ATAAGCG 220 0.0 43.977276 10 TTACGGG 1145 0.0 43.03493 3 TCTTACG 175 0.0 42.428574 1 CTTACGG 345 0.0 42.3913 2 TACGGGA 765 0.0 42.352943 4 GCTCGAT 80 0.0 42.187504 27 GCTTACG 135 0.0 41.666668 1 GTTTACG 130 0.0 41.53846 1 TCGCCTA 60 3.6379788E-12 41.250004 22 TATGGGA 2255 0.0 40.410202 4 TAAGCGT 195 0.0 40.384617 11 >>END_MODULE