SRR2935312.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935312.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	561993
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGCT	3425	0.6094381958494145	TruSeq Adapter, Index 27 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC	3315	0.5898649983184844	TruSeq Adapter, Index 20 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC	2827	0.5030311765449036	TruSeq Adapter, Index 20 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC	2262	0.4024961164996717	TruSeq Adapter, Index 20 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTG	1145	0.20373919248104513	TruSeq Adapter, Index 27 (95% over 21bp)
TTCCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCT	749	0.13327568136969678	No Hit
TTATTGGGGATCACACGGTAGCAGAGATAGGCATTTTATGTGTTCCTTATA	694	0.12348908260423172	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	652	0.11601567991060385	No Hit
TGCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTG	642	0.11423629831688294	TruSeq Adapter, Index 27 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTG	613	0.10907609169509228	No Hit
TGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGCTT	592	0.10533939034827836	TruSeq Adapter, Index 20 (95% over 23bp)
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	590	0.10498351402953418	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCCT	60	0.0	45.000004	25
ACGCCCA	30	2.1635133E-6	45.000004	1
TTTACGG	135	0.0	45.000004	2
ACGTTCC	30	2.1635133E-6	45.000004	20
TCACACG	180	0.0	45.000004	11
ATCACTC	30	2.1635133E-6	45.000004	36
GTAATTA	30	2.1635133E-6	45.000004	45
CCCCGAC	30	2.1635133E-6	45.000004	9
ATAAGCG	30	2.1635133E-6	45.000004	33
ACTCGTA	30	2.1635133E-6	45.000004	14
CTACCGA	30	2.1635133E-6	45.000004	25
TCGTAAG	30	2.1635133E-6	45.000004	16
TAGTGAT	30	2.1635133E-6	45.000004	16
TACGTTG	30	2.1635133E-6	45.000004	1
TCACGCC	20	7.0302055E-4	45.0	16
AACGTAG	20	7.0302055E-4	45.0	8
GTCGCTC	20	7.0302055E-4	45.0	23
AACCGAC	20	7.0302055E-4	45.0	10
ACACGAT	20	7.0302055E-4	45.0	12
CGAACAA	20	7.0302055E-4	45.0	13