##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935312.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	561993
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26260647374612	33.0	31.0	34.0	30.0	34.0
2	32.41640020427301	34.0	31.0	34.0	30.0	34.0
3	32.47496677004874	34.0	31.0	34.0	30.0	34.0
4	35.93056853021301	37.0	35.0	37.0	35.0	37.0
5	31.890765543343065	37.0	35.0	37.0	0.0	37.0
6	33.51552065595123	37.0	35.0	37.0	17.0	37.0
7	35.237006510757254	37.0	35.0	37.0	32.0	37.0
8	35.76637253488922	37.0	35.0	37.0	35.0	37.0
9	37.762569996423444	39.0	38.0	39.0	35.0	39.0
10	37.502936869320436	39.0	37.0	39.0	35.0	39.0
11	37.41399981850308	39.0	37.0	39.0	35.0	39.0
12	37.033594724489454	39.0	35.0	39.0	34.0	39.0
13	36.85906408086934	39.0	35.0	39.0	33.0	39.0
14	37.859738466493354	40.0	36.0	41.0	33.0	41.0
15	38.025872208372704	40.0	36.0	41.0	33.0	41.0
16	38.12842686652681	40.0	36.0	41.0	34.0	41.0
17	38.1725786620118	40.0	36.0	41.0	34.0	41.0
18	38.160631893991564	40.0	36.0	41.0	34.0	41.0
19	38.17576909320935	40.0	36.0	41.0	34.0	41.0
20	38.07020550078026	40.0	36.0	41.0	34.0	41.0
21	37.99531844702692	40.0	36.0	41.0	34.0	41.0
22	37.97724170941631	40.0	36.0	41.0	34.0	41.0
23	37.93632127090551	40.0	36.0	41.0	34.0	41.0
24	37.91353451021632	40.0	36.0	41.0	34.0	41.0
25	37.794559718715355	39.0	35.0	41.0	34.0	41.0
26	37.774918904683865	40.0	35.0	41.0	34.0	41.0
27	37.72617274592388	39.0	35.0	41.0	34.0	41.0
28	37.708391385657826	39.0	35.0	41.0	34.0	41.0
29	37.665058105705945	39.0	35.0	41.0	33.0	41.0
30	37.60027793940494	39.0	35.0	41.0	33.0	41.0
31	37.398857281140515	39.0	35.0	41.0	33.0	41.0
32	37.4077862179778	39.0	35.0	41.0	33.0	41.0
33	37.38428414588794	39.0	35.0	41.0	33.0	41.0
34	37.33588140777554	39.0	35.0	41.0	33.0	41.0
35	37.28380424667211	39.0	35.0	41.0	33.0	41.0
36	37.24029836670564	39.0	35.0	41.0	33.0	41.0
37	37.19697042489853	39.0	35.0	41.0	33.0	41.0
38	37.16135254353702	39.0	35.0	41.0	33.0	41.0
39	37.13375077625522	39.0	35.0	41.0	33.0	41.0
40	37.032238835715034	39.0	35.0	41.0	33.0	41.0
41	36.998514216369244	39.0	35.0	41.0	32.0	41.0
42	37.01640589829411	39.0	35.0	41.0	33.0	41.0
43	36.93683195342291	39.0	35.0	41.0	32.0	41.0
44	36.859820318046665	39.0	35.0	41.0	32.0	41.0
45	36.838949951333916	39.0	35.0	40.0	32.0	41.0
46	36.74650573939533	38.0	35.0	40.0	32.0	41.0
47	36.7031279749036	38.0	35.0	40.0	32.0	41.0
48	36.6832362680674	38.0	35.0	40.0	32.0	41.0
49	36.65280706343317	38.0	35.0	40.0	32.0	41.0
50	36.54677549364494	38.0	35.0	40.0	32.0	41.0
51	35.903810189806634	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	8.0
10	9.0
11	11.0
12	9.0
13	11.0
14	15.0
15	14.0
16	21.0
17	43.0
18	83.0
19	134.0
20	230.0
21	420.0
22	614.0
23	922.0
24	1406.0
25	2084.0
26	2785.0
27	3598.0
28	4406.0
29	5752.0
30	7611.0
31	10316.0
32	14692.0
33	22855.0
34	40114.0
35	58784.0
36	46806.0
37	71884.0
38	114575.0
39	151666.0
40	110.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.106134773920672	4.563579973416039	56.81921305069636	17.51107220196693
2	18.61090796504583	4.500233988679574	55.24428240209398	21.644575644180623
3	20.485486474030814	4.865007215392363	53.21721089052711	21.432295420049716
4	18.62549889411434	4.642050701699132	49.930515148765195	26.801935255421334
5	16.114969403533497	16.47315891834952	43.302140773995404	24.10973090412158
6	20.100250358990237	6.509689622468608	52.67236424652976	20.717695772011396
7	81.71276154685201	2.5398892868772385	10.136781063109327	5.610568103161427
8	82.87291834595804	2.714802497540005	8.070385218321224	6.341893938180725
9	76.82586793785687	5.621244392723753	10.902982777365553	6.649904892053815
10	41.77863425345156	25.06205593308102	16.58917459826012	16.57013521520731
11	32.832615352860266	23.054023804566963	24.670058167984298	19.443302674588473
12	28.919577290108595	19.515011752815425	29.73079735868596	21.834613598390014
13	23.508477863603282	25.22415759626899	31.679042265651	19.588322274476727
14	18.092040292316806	29.177231744879386	31.55679875016237	21.173929212641436
15	15.129192000612107	23.682145507150445	39.90334399182908	21.28531850040837
16	17.559293443156765	22.677506659335613	35.27090195073604	24.49229794677158
17	18.936534796696755	21.512011715448413	29.52421115565496	30.027242332199865
18	20.986560330822627	22.116823519154153	31.595411330746114	25.301204819277107
19	24.709738377524275	24.198344107488882	28.921178733542945	22.170738781443898
20	25.337682141948388	26.031640963499548	27.494648509856884	21.136028384695184
21	24.14442884519914	25.585372059794338	28.53362942243053	21.736569672575992
22	21.295816851811324	24.097453171124908	28.139318461261976	26.467411515801796
23	21.404003252709554	25.917404665182666	25.849254350143152	26.829337731964632
24	22.911317400750544	23.118081541940914	28.880964709524854	25.08963634778369
25	20.27569738413112	25.092839234652388	27.025781459911425	27.60568192130507
26	18.60521394394592	26.898377737801006	27.394113449811652	27.102294868441422
27	19.572841654611356	25.96064363791008	29.681864364858633	24.784650342619926
28	17.742925623628764	23.967202438464536	32.16018704859313	26.12968488931357
29	19.784943940582888	22.502771386832222	30.516216394154377	27.196068278430513
30	20.863249186377768	23.751897265624304	30.69006197586091	24.69479157213702
31	23.58890591163947	24.756536113439136	27.485929540047653	24.168628434873742
32	23.553496217924422	24.755112608164158	27.310126638588024	24.381264535323393
33	24.99853201018518	22.919502556081657	28.008889790442232	24.07307564329093
34	20.992254351922533	21.86557483812076	30.505006290113933	26.637164519842777
35	20.68299783093384	22.260241675608057	29.48986909089615	27.566891402561954
36	23.341038055634144	21.983369899625085	30.565861140619187	24.10973090412158
37	21.459164082114903	24.469699800531323	31.043625098533255	23.02751101882052
38	22.590850775721407	24.72735425530211	27.923657412102997	24.758137556873482
39	23.992291718936002	22.299032194351177	29.946102531526194	23.76257355518663
40	22.607043148224264	21.518417489185808	32.278871800894315	23.595667561695606
41	19.095433573016035	22.359175292218943	30.598779700103023	27.946611434662
42	22.749927490200054	20.97428259782595	29.493071977764846	26.782717934209145
43	24.587494862035648	20.69491968761177	29.016375648807013	25.70120980154557
44	21.87589525136434	20.93139950141728	29.08683915991836	28.10586608730002
45	22.037107223755456	22.50971097504773	27.337351176971957	28.11583062422486
46	22.710603156978824	21.405960572462646	31.263022849039046	24.620413421519487
47	18.17549328906232	22.22287466213992	33.68582882704945	25.915803221748313
48	20.915918881551907	20.36181945326721	32.56819924803334	26.154062417147543
49	23.28605516438817	18.729058902868896	32.873719067675225	25.111166865067712
50	20.959335792438697	20.284594292099722	31.206616452518094	27.54945346294349
51	19.198281829133105	21.20969478267523	28.937371106045806	30.65465228214586
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	196.0
1	332.5
2	469.0
3	708.5
4	948.0
5	719.5
6	491.0
7	589.0
8	687.0
9	861.5
10	1036.0
11	1271.5
12	1507.0
13	1474.0
14	1441.0
15	1455.0
16	1469.0
17	1423.0
18	1377.0
19	1322.0
20	1267.0
21	1321.0
22	1375.0
23	1507.5
24	1640.0
25	1695.5
26	2104.0
27	2457.0
28	3571.5
29	4686.0
30	5421.0
31	6156.0
32	6906.0
33	7656.0
34	8208.0
35	8760.0
36	10351.0
37	11942.0
38	14200.5
39	16459.0
40	20406.0
41	24353.0
42	29708.5
43	35064.0
44	39597.0
45	44130.0
46	52424.0
47	60718.0
48	68457.5
49	76197.0
50	70189.5
51	64182.0
52	53517.5
53	42853.0
54	36520.0
55	30187.0
56	26783.5
57	23380.0
58	23088.5
59	22797.0
60	21572.5
61	20348.0
62	17612.5
63	14877.0
64	12560.5
65	10244.0
66	8175.5
67	6107.0
68	5206.0
69	4305.0
70	3847.0
71	3389.0
72	2772.0
73	2155.0
74	1792.0
75	1012.0
76	595.0
77	551.5
78	508.0
79	372.0
80	236.0
81	168.0
82	100.0
83	70.5
84	41.0
85	23.0
86	5.0
87	5.5
88	6.0
89	5.0
90	4.0
91	8.0
92	12.0
93	6.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	561993.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.990150502770593
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.99778476420413	18.082802941381
2	12.663997569883845	6.836064007557721
3	5.748232294653776	4.654369642727754
4	3.2099293854262134	3.465459088636776
5	2.114593489505619	2.8536598266967754
6	1.4031103875554598	2.2722096319273577
7	1.0008899446068105	1.8909919169153275
8	0.7921679962522181	1.7104586753860465
9	0.5804623905603854	1.4100090554180529
>10	4.09836719899375	24.125205627823785
>50	1.2134363089991642	22.367371834548834
>100	0.16844107718823456	7.114079008393197
>500	0.005284425951003437	0.9042580440210083
>1k	0.003302766219377148	2.313060698566357
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGCT	3425	0.6094381958494145	TruSeq Adapter, Index 27 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC	3315	0.5898649983184844	TruSeq Adapter, Index 20 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC	2827	0.5030311765449036	TruSeq Adapter, Index 20 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC	2262	0.4024961164996717	TruSeq Adapter, Index 20 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTG	1145	0.20373919248104513	TruSeq Adapter, Index 27 (95% over 21bp)
TTCCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCT	749	0.13327568136969678	No Hit
TTATTGGGGATCACACGGTAGCAGAGATAGGCATTTTATGTGTTCCTTATA	694	0.12348908260423172	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	652	0.11601567991060385	No Hit
TGCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTG	642	0.11423629831688294	TruSeq Adapter, Index 27 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTG	613	0.10907609169509228	No Hit
TGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGCTT	592	0.10533939034827836	TruSeq Adapter, Index 20 (95% over 23bp)
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	590	0.10498351402953418	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7793815937209182E-4	0.0	0.0	0.6268761354678795	0.0
2	1.7793815937209182E-4	0.0	0.0	2.2196006000074733	0.0
3	1.7793815937209182E-4	0.0	0.0	3.0619598464749562	0.0
4	1.7793815937209182E-4	0.0	0.0	4.041331475658949	0.0
5	1.7793815937209182E-4	0.0	0.0	5.727829350187636	0.0
6	1.7793815937209182E-4	0.0	0.0	6.889409654568651	0.0
7	1.7793815937209182E-4	0.0	0.0	7.954547476569993	0.0
8	1.7793815937209182E-4	0.0	0.0	9.727701234712887	0.0
9	1.7793815937209182E-4	0.0	0.0	10.626288939541952	0.0
10	3.5587631874418364E-4	0.0	0.0	12.185027215641476	0.0
11	3.5587631874418364E-4	0.0	0.0	14.755343927771342	0.0
12	3.5587631874418364E-4	0.0	0.0	17.113736292089047	0.0
13	3.5587631874418364E-4	0.0	0.0	17.796485009599763	0.0
14	3.5587631874418364E-4	0.0	0.0	18.03011781285532	0.0
15	3.5587631874418364E-4	0.0	0.0	18.388663203990085	0.0
16	3.5587631874418364E-4	0.0	0.0	19.331913386821544	0.0
17	3.5587631874418364E-4	0.0	0.0	20.715916390417675	0.0
18	3.5587631874418364E-4	0.0	0.0	22.420919833521058	0.0
19	3.5587631874418364E-4	0.0	0.0	23.359543624208843	0.0
20	3.5587631874418364E-4	0.0	0.0	24.41062433161979	0.0
21	5.338144781162755E-4	0.0	0.0	25.702455368661177	0.0
22	5.338144781162755E-4	0.0	0.0	26.91065547079768	0.0
23	5.338144781162755E-4	0.0	0.0	28.08131062130667	0.0
24	5.338144781162755E-4	0.0	0.0	28.95658842725799	0.0
25	5.338144781162755E-4	0.0	0.0	29.679907045105544	0.0
26	5.338144781162755E-4	0.0	0.0	30.3158580267014	0.0
27	5.338144781162755E-4	0.0	0.0	31.01586674567121	0.0
28	5.338144781162755E-4	0.0	0.0	31.670501234001136	0.0
29	5.338144781162755E-4	0.0	0.0	32.368908509536595	0.0
30	5.338144781162755E-4	0.0	0.0	33.18777991896696	0.0
31	7.117526374883673E-4	0.0	0.0	33.925155651404914	0.0
32	7.117526374883673E-4	0.0	0.0	34.64651694949937	0.0
33	7.117526374883673E-4	0.0	0.0	35.27232545601102	0.0
34	7.117526374883673E-4	0.0	0.0	35.92055417060355	0.0
35	7.117526374883673E-4	0.0	0.0	36.623765776442056	0.0
36	7.117526374883673E-4	0.0	0.0	37.30135428733098	0.0
37	7.117526374883673E-4	0.0	0.0	37.95936960068898	0.0
38	7.117526374883673E-4	0.0	0.0	38.61044532583146	0.0
39	7.117526374883673E-4	0.0	0.0	39.39710992841548	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCCT	60	0.0	45.000004	25
ACGCCCA	30	2.1635133E-6	45.000004	1
TTTACGG	135	0.0	45.000004	2
ACGTTCC	30	2.1635133E-6	45.000004	20
TCACACG	180	0.0	45.000004	11
ATCACTC	30	2.1635133E-6	45.000004	36
GTAATTA	30	2.1635133E-6	45.000004	45
CCCCGAC	30	2.1635133E-6	45.000004	9
ATAAGCG	30	2.1635133E-6	45.000004	33
ACTCGTA	30	2.1635133E-6	45.000004	14
CTACCGA	30	2.1635133E-6	45.000004	25
TCGTAAG	30	2.1635133E-6	45.000004	16
TAGTGAT	30	2.1635133E-6	45.000004	16
TACGTTG	30	2.1635133E-6	45.000004	1
TCACGCC	20	7.0302055E-4	45.0	16
AACGTAG	20	7.0302055E-4	45.0	8
GTCGCTC	20	7.0302055E-4	45.0	23
AACCGAC	20	7.0302055E-4	45.0	10
ACACGAT	20	7.0302055E-4	45.0	12
CGAACAA	20	7.0302055E-4	45.0	13
>>END_MODULE