##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935311.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1064184
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.403349420776856	33.0	31.0	34.0	31.0	34.0
2	32.555174669042195	34.0	31.0	34.0	31.0	34.0
3	32.605586064064106	34.0	31.0	34.0	31.0	34.0
4	36.0181068311495	37.0	35.0	37.0	35.0	37.0
5	32.14217560121182	37.0	35.0	37.0	0.0	37.0
6	33.65921964622659	37.0	35.0	37.0	17.0	37.0
7	35.32772809965194	37.0	35.0	37.0	32.0	37.0
8	35.82912165565353	37.0	35.0	37.0	35.0	37.0
9	37.84771336535787	39.0	38.0	39.0	35.0	39.0
10	37.62520861054104	39.0	37.0	39.0	35.0	39.0
11	37.50470501341873	39.0	37.0	39.0	35.0	39.0
12	37.29855645264353	39.0	37.0	39.0	35.0	39.0
13	37.24543687933666	39.0	37.0	39.0	34.0	39.0
14	38.47578238349759	40.0	38.0	41.0	34.0	41.0
15	38.57042673071574	40.0	38.0	41.0	35.0	41.0
16	38.63656285003345	40.0	38.0	41.0	35.0	41.0
17	38.63046146155176	40.0	38.0	41.0	35.0	41.0
18	38.62326439788608	40.0	38.0	41.0	35.0	41.0
19	38.62655142343805	40.0	38.0	41.0	35.0	41.0
20	38.55596682528585	40.0	38.0	41.0	35.0	41.0
21	38.50733801673395	40.0	38.0	41.0	34.0	41.0
22	38.453849146388215	40.0	37.0	41.0	34.0	41.0
23	38.41774918623095	40.0	37.0	41.0	34.0	41.0
24	38.395655262623755	40.0	37.0	41.0	34.0	41.0
25	38.30782646609985	40.0	37.0	41.0	34.0	41.0
26	38.274554964179124	40.0	37.0	41.0	34.0	41.0
27	38.19824767145531	40.0	37.0	41.0	34.0	41.0
28	38.19730328589793	40.0	37.0	41.0	34.0	41.0
29	38.13352390188163	40.0	37.0	41.0	34.0	41.0
30	38.06227400524721	40.0	37.0	41.0	34.0	41.0
31	37.90225656465423	40.0	36.0	41.0	34.0	41.0
32	37.91615077843681	40.0	36.0	41.0	34.0	41.0
33	37.89112691038392	40.0	36.0	41.0	34.0	41.0
34	37.86331780970208	40.0	36.0	41.0	34.0	41.0
35	37.78882505281042	40.0	36.0	41.0	33.0	41.0
36	37.769818941085376	40.0	36.0	41.0	33.0	41.0
37	37.72672582936785	40.0	36.0	41.0	33.0	41.0
38	37.71407388196026	40.0	36.0	41.0	33.0	41.0
39	37.70150368733226	40.0	36.0	41.0	33.0	41.0
40	37.63623489922795	40.0	36.0	41.0	33.0	41.0
41	37.584943957060055	40.0	35.0	41.0	33.0	41.0
42	37.5503070897514	40.0	35.0	41.0	33.0	41.0
43	37.47042146846786	39.0	35.0	41.0	33.0	41.0
44	37.404293806334245	39.0	35.0	41.0	33.0	41.0
45	37.4012454613112	39.0	35.0	41.0	33.0	41.0
46	37.306223359870096	39.0	35.0	41.0	33.0	41.0
47	37.25927753095329	39.0	35.0	41.0	33.0	41.0
48	37.21239278169941	39.0	35.0	41.0	33.0	41.0
49	37.1897763920525	39.0	35.0	41.0	33.0	41.0
50	37.097656984130566	39.0	35.0	41.0	33.0	41.0
51	36.43723829713659	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	14.0
11	13.0
12	12.0
13	24.0
14	14.0
15	19.0
16	39.0
17	70.0
18	90.0
19	179.0
20	373.0
21	642.0
22	983.0
23	1470.0
24	2105.0
25	3148.0
26	4365.0
27	5663.0
28	6727.0
29	8571.0
30	11532.0
31	15844.0
32	21839.0
33	34094.0
34	59223.0
35	88754.0
36	84669.0
37	132695.0
38	222622.0
39	357953.0
40	431.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.17860445186171	4.785356667643941	55.6771197462093	16.35891913428505
2	20.366402802522874	5.139336806416935	53.43258308713531	21.061677303924885
3	20.86039632244048	5.063879930538328	51.69632319222992	22.379400554791275
4	18.672146921960863	5.479973388060711	50.72055208497779	25.127327605000637
5	16.562925208422605	16.334393300406695	44.51852311254444	22.584158378626253
6	21.564315945362832	7.452000781819685	52.43754839388677	18.54613487893071
7	82.18428392082572	2.556982627064493	10.084440284762785	5.174293167347
8	84.4587026303722	3.3479172774632957	6.918540402787487	5.274839689377025
9	78.42750877667771	5.679374995301564	9.581895612037016	6.311220615983702
10	40.015166550145466	25.201750825045295	18.314408034700765	16.468674590108478
11	30.17899160295588	25.881426520225826	23.90282131661442	20.036760560203877
12	25.696402125948143	22.361076655916644	29.6261736692151	22.316347548920113
13	23.733019853709507	25.099982709756958	30.151834645136553	21.01516279139698
14	20.563643129383642	27.28616479856867	29.620817452620972	22.529374619426715
15	18.66632086180585	24.56811979883178	33.98782541365027	22.777733925712095
16	20.40004360148245	23.358742473106155	32.221401562135874	24.019812363275523
17	19.6201972591206	23.944167549972562	29.07561098456658	27.360024206340256
18	20.993362050171775	22.848586334694	32.052163911504024	24.1058877036302
19	22.848022522420937	24.512114446373936	29.660660186584277	22.979202844620854
20	24.969272231117927	24.86440314832773	29.265239845740965	20.901084774813377
21	24.53344534403825	26.621242191200018	27.95259090533216	20.89272155942957
22	22.663186065567608	25.65007555084459	27.287198454402624	24.39953992918518
23	21.53076911511543	25.23755290438496	27.613457823083227	25.61822015741639
24	22.511238657976442	24.422092420107802	28.82415071077934	24.24251821113642
25	20.924764890282134	25.663231170549455	28.250283785510778	25.16172015365764
26	19.713038346752064	26.620960285063482	29.360899994737753	24.305101373446696
27	20.43603361824647	28.0300211241665	29.082846575404254	22.45109868218278
28	20.705723822196163	25.270629891071465	30.53193808589492	23.49170820083745
29	21.77640332874766	24.22052953248686	30.476120670861427	23.526946467904047
30	22.244837358952964	27.808724806988266	28.42901227607256	21.517425557986215
31	24.10663945332762	27.21662795155725	26.417424054486816	22.25930854062831
32	23.90357306631184	26.96488577163348	26.18231433661848	22.949226825436202
33	24.14751584312487	27.116457304375935	25.865733745292168	22.870293107207022
34	21.74078918683235	25.637953586973683	28.45128286085865	24.169974365335317
35	20.524364207693406	24.77738812085128	28.689211640092317	26.009036031362996
36	23.3405125429437	26.65901761349541	27.910211016140067	22.090258827420822
37	22.965483412642925	27.41339843485713	28.730275967313922	20.89084218518602
38	23.37095840568924	27.364816610661315	25.760300850228905	23.503924133420536
39	23.254907046149913	26.108642866271246	27.882866120896388	22.753583966682452
40	22.24934785713749	24.942867022996023	29.340978627756105	23.466806492110386
41	20.93810844741135	23.789025206167356	28.271802620599445	27.00106372582185
42	22.50569450395796	24.270802793501876	27.010742503176143	26.21276019936402
43	24.11453282515054	24.450001127624546	27.714662126098492	23.72080392112642
44	22.2044308127166	25.09678777354292	27.85862219315457	24.840159220585914
45	21.77997413981041	25.214530569901445	27.137130421055005	25.86836486923314
46	22.64551994767822	25.45330506754471	28.778293979236675	23.122881005540396
47	20.637408568443053	25.43075257662209	30.29532486863174	23.63651398630312
48	21.807131097629735	23.941254519895054	29.152853265976557	25.098761116498654
49	23.32143689437165	22.643828510859024	30.070081865542047	23.964652729227275
50	22.541308645873272	22.79041970185607	29.132180149297486	25.536091502973168
51	20.562797410974042	23.310630492471226	27.619659758086947	28.50691233846778
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	237.0
1	569.0
2	901.0
3	1422.0
4	1943.0
5	1481.0
6	1019.0
7	1145.0
8	1271.0
9	1403.5
10	1536.0
11	1652.0
12	1768.0
13	1835.5
14	1903.0
15	2012.5
16	2122.0
17	1985.5
18	1849.0
19	2011.5
20	2174.0
21	2849.0
22	3524.0
23	3666.5
24	3809.0
25	5053.0
26	7302.0
27	8307.0
28	9773.5
29	11240.0
30	12630.5
31	14021.0
32	17239.5
33	20458.0
34	24236.5
35	28015.0
36	29887.5
37	31760.0
38	34728.5
39	37697.0
40	45155.5
41	52614.0
42	58837.5
43	65061.0
44	72507.5
45	79954.0
46	91571.0
47	103188.0
48	113951.5
49	124715.0
50	120689.0
51	116663.0
52	98486.5
53	80310.0
54	70150.0
55	59990.0
56	54251.0
57	48512.0
58	44172.5
59	39833.0
60	35862.0
61	31891.0
62	28267.5
63	24644.0
64	21672.0
65	18700.0
66	15161.0
67	11622.0
68	10049.0
69	8476.0
70	7613.0
71	6750.0
72	5229.5
73	3709.0
74	3078.5
75	2183.5
76	1919.0
77	1280.0
78	641.0
79	517.5
80	394.0
81	277.0
82	160.0
83	106.5
84	53.0
85	65.0
86	77.0
87	42.5
88	8.0
89	4.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1064184.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.949725307209427
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.92932547152478	17.627473362900922
2	12.520421153069433	6.498029793054521
3	5.381582931658937	4.189517963835486
4	2.943753982688857	3.0555842889111826
5	1.8878490955476845	2.449458272546308
6	1.2842732704305042	1.9995923152237842
7	0.9122669467877009	1.6571153673191235
8	0.6854518351394024	1.4229829466552075
9	0.5508618109020748	1.2865241407626702
>10	4.367382838995533	26.302616443776607
>50	1.3725475321767417	24.170185175955147
>100	0.15699784810370557	6.5311480745447
>500	0.004735433933521564	0.9207733434760226
>1k	0.002185584892394568	1.2917861114817086
>5k	3.6426414873242795E-4	0.5972123995566687
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGC	6318	0.5936943235380349	No Hit
CTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGCT	4155	0.39043999909790034	No Hit
GCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGC	3057	0.28726235312690285	No Hit
TCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGC	3014	0.28322169850326634	No Hit
TCCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTG	1303	0.12244123196740414	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGC	1084	0.1018620840005112	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.396871217759335E-5	0.0	0.0	0.4014343384226788	0.0
2	9.396871217759335E-5	0.0	0.0	1.6299812814325343	0.0
3	9.396871217759335E-5	0.0	0.0	2.2230178239853258	0.0
4	9.396871217759335E-5	0.0	0.0	2.860783473534652	0.0
5	9.396871217759335E-5	0.0	0.0	4.060951865466874	0.0
6	9.396871217759335E-5	0.0	0.0	4.853014140411808	0.0
7	9.396871217759335E-5	0.0	0.0	5.5873796260797	0.0
8	9.396871217759335E-5	0.0	0.0	6.785668643768371	0.0
9	9.396871217759335E-5	0.0	0.0	7.413379641114695	0.0
10	9.396871217759335E-5	0.0	0.0	8.593062853792201	0.0
11	9.396871217759335E-5	0.0	0.0	10.503352803650497	0.0
12	9.396871217759335E-5	0.0	0.0	12.196199153529841	0.0
13	9.396871217759335E-5	0.0	0.0	12.732102719078656	0.0
14	9.396871217759335E-5	0.0	0.0	12.939209760718072	0.0
15	9.396871217759335E-5	0.0	0.0	13.2590792569706	0.0
16	9.396871217759335E-5	0.0	0.0	14.023514730535322	0.0
17	9.396871217759335E-5	0.0	0.0	15.087898333370921	0.0
18	9.396871217759335E-5	0.0	0.0	16.337212361772025	0.0
19	9.396871217759335E-5	0.0	0.0	17.13303338520406	0.0
20	9.396871217759335E-5	0.0	0.0	17.935150312352	0.0
21	9.396871217759335E-5	0.0	0.0	18.974350300324005	0.0
22	1.879374243551867E-4	0.0	0.0	19.99748163851364	0.0
23	1.879374243551867E-4	0.0	0.0	21.00294685881389	0.0
24	1.879374243551867E-4	0.0	0.0	21.83184486893244	0.0
25	1.879374243551867E-4	0.0	0.0	22.52796508874405	0.0
26	1.879374243551867E-4	0.0	0.0	23.15971674071401	0.0
27	1.879374243551867E-4	0.0	0.0	23.76900987047353	0.0
28	1.879374243551867E-4	0.0	0.0	24.40461423964277	0.0
29	1.879374243551867E-4	0.0	0.0	25.067093660494802	0.0
30	1.879374243551867E-4	0.0	0.0	25.755696383332207	0.0
31	1.879374243551867E-4	0.0	0.0	26.40483224705502	0.0
32	2.8190613653278004E-4	0.0	0.0	27.046074738954918	0.0
33	2.8190613653278004E-4	0.0	0.0	27.64108462446344	0.0
34	2.8190613653278004E-4	0.0	0.0	28.25451237755877	0.0
35	2.8190613653278004E-4	0.0	0.0	28.911353675680147	0.0
36	3.758748487103734E-4	0.0	0.0	29.511625809070612	0.0
37	3.758748487103734E-4	0.0	0.0	30.113683347992453	0.0
38	3.758748487103734E-4	0.0	0.0	30.725701570405118	0.0
39	3.758748487103734E-4	0.0	0.0	31.317516519699602	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGATA	65	0.0	45.000004	14
TCGCATA	20	7.0330506E-4	45.0	31
TCGCAAG	20	7.0330506E-4	45.0	12
CGACGAT	20	7.0330506E-4	45.0	15
CTATCGA	20	7.0330506E-4	45.0	42
ATCCGGC	25	3.890611E-5	45.0	12
AATGACG	20	7.0330506E-4	45.0	34
AACATCG	20	7.0330506E-4	45.0	23
AGGTTAC	25	3.890611E-5	45.0	41
AGATTCG	35	1.2118107E-7	45.0	40
ACTCGTC	25	3.890611E-5	45.0	37
CATACGA	50	2.1827873E-11	45.0	18
ACGTCTA	20	7.0330506E-4	45.0	34
CATCGGT	20	7.0330506E-4	45.0	33
GGCATCG	20	7.0330506E-4	45.0	10
GACGCGT	20	7.0330506E-4	45.0	42
TGAATCG	25	3.890611E-5	45.0	28
GTTATCG	25	3.890611E-5	45.0	1
TACGTTG	20	7.0330506E-4	45.0	1
TACGTCA	20	7.0330506E-4	45.0	36
>>END_MODULE