##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935310.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1174071
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37269807362587	33.0	31.0	34.0	30.0	34.0
2	32.52526210084399	34.0	31.0	34.0	31.0	34.0
3	32.578495678711086	34.0	31.0	34.0	31.0	34.0
4	35.998451541687004	37.0	35.0	37.0	35.0	37.0
5	32.18101205123029	37.0	35.0	37.0	0.0	37.0
6	33.6691256320955	37.0	35.0	37.0	17.0	37.0
7	35.31222302569436	37.0	35.0	37.0	32.0	37.0
8	35.81146029499068	37.0	35.0	37.0	35.0	37.0
9	37.81396951291702	39.0	38.0	39.0	35.0	39.0
10	37.58489648411382	39.0	37.0	39.0	35.0	39.0
11	37.50755874218851	39.0	37.0	39.0	35.0	39.0
12	37.18503139929357	39.0	37.0	39.0	34.0	39.0
13	37.06114962383025	39.0	37.0	39.0	33.0	39.0
14	38.094862235759166	40.0	37.0	41.0	33.0	41.0
15	38.230400887169516	40.0	37.0	41.0	33.0	41.0
16	38.33991044834597	40.0	37.0	41.0	34.0	41.0
17	38.37193236184183	40.0	37.0	41.0	34.0	41.0
18	38.349819559464464	40.0	37.0	41.0	34.0	41.0
19	38.36573852858984	40.0	37.0	41.0	34.0	41.0
20	38.28485670798444	40.0	37.0	41.0	34.0	41.0
21	38.208977991961305	40.0	37.0	41.0	34.0	41.0
22	38.21682930589377	40.0	37.0	41.0	34.0	41.0
23	38.17015921524337	40.0	37.0	41.0	34.0	41.0
24	38.14544009689363	40.0	36.0	41.0	34.0	41.0
25	38.05842661985519	40.0	36.0	41.0	34.0	41.0
26	37.998376588809364	40.0	36.0	41.0	34.0	41.0
27	37.92956558845249	40.0	36.0	41.0	34.0	41.0
28	37.903448769282264	40.0	36.0	41.0	34.0	41.0
29	37.83851487686861	40.0	36.0	41.0	34.0	41.0
30	37.76121461138211	40.0	36.0	41.0	33.0	41.0
31	37.59049495303095	40.0	35.0	41.0	33.0	41.0
32	37.589321259106136	40.0	35.0	41.0	33.0	41.0
33	37.54482395016996	39.0	35.0	41.0	33.0	41.0
34	37.441631724146156	39.0	35.0	41.0	33.0	41.0
35	37.355240867034446	39.0	35.0	41.0	33.0	41.0
36	37.34130900090369	39.0	35.0	41.0	33.0	41.0
37	37.329233070231695	39.0	35.0	41.0	33.0	41.0
38	37.333904849025316	39.0	35.0	41.0	33.0	41.0
39	37.315240730756486	39.0	35.0	41.0	33.0	41.0
40	37.19296873868787	39.0	35.0	41.0	33.0	41.0
41	37.14209106604285	39.0	35.0	41.0	33.0	41.0
42	37.140996583681904	39.0	35.0	41.0	33.0	41.0
43	37.060423091959514	39.0	35.0	41.0	32.0	41.0
44	36.99452077429729	39.0	35.0	41.0	32.0	41.0
45	36.963175140174656	39.0	35.0	41.0	32.0	41.0
46	36.90014147355654	39.0	35.0	41.0	32.0	41.0
47	36.85868742179987	39.0	35.0	41.0	32.0	41.0
48	36.833932530485804	39.0	35.0	41.0	32.0	41.0
49	36.80032212702639	39.0	35.0	41.0	32.0	41.0
50	36.701025747165204	38.0	35.0	40.0	32.0	41.0
51	36.05851434879151	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	6.0
10	10.0
11	15.0
12	16.0
13	29.0
14	33.0
15	36.0
16	48.0
17	113.0
18	160.0
19	298.0
20	506.0
21	811.0
22	1355.0
23	2028.0
24	2850.0
25	4299.0
26	5606.0
27	7214.0
28	9130.0
29	11462.0
30	14816.0
31	20432.0
32	28507.0
33	44549.0
34	79740.0
35	102231.0
36	96037.0
37	148571.0
38	241929.0
39	350763.0
40	468.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.07971919926478	4.785570889665106	52.67160163226926	18.463108278800856
2	19.568492876495544	5.146281613292552	52.55832057856807	22.726904931643826
3	21.19897348627127	5.122432970408093	51.20073658237023	22.4778569609504
4	19.703152535068153	5.038025809342025	50.13231738114645	25.126504274443366
5	16.3343613801891	16.35344029449667	44.52354244334457	22.78865588196966
6	21.369661630344332	7.168391008720938	51.64704689920797	19.81490046172676
7	82.27219648556178	2.835177770339272	9.629315433223375	5.263310310875577
8	83.44376106726084	3.264197821085778	7.575010369900968	5.717030741752415
9	77.5652409436908	5.6720590151702925	10.28983766739831	6.4728623737405995
10	39.014420763309886	27.949331854717474	16.964732115860116	16.071515266112527
11	33.055155948831036	24.495622496424833	23.601553909431374	18.847667645312765
12	29.11663775018717	22.13537341438465	28.291304358935704	20.45668447649248
13	25.538404406547816	24.51631971149956	29.59216265455837	20.353113227394253
14	19.97528258512475	27.648924128097875	31.01728941435399	21.358503872423388
15	18.893320761691584	23.596869354579066	36.34822766255192	21.161582221177426
16	21.578337255583353	24.510783419401385	32.20043762259693	21.710441702418336
17	22.759781989334545	23.634516140846678	28.40407437028936	25.201627499529415
18	23.86397415488501	22.565671070999965	30.906137703767488	22.664217070347533
19	25.6954647546869	24.415729542761895	27.766719389202187	22.122086313349023
20	26.863707561127054	26.84062548176388	26.86438895092375	19.431278006185316
21	24.82899245445974	26.20335567440129	28.845018742478096	20.122633128660876
22	23.079268630261712	24.328426475059857	29.082823781525992	23.509481113152443
23	22.039212279325525	26.343381277622903	28.088676068142387	23.528730374909184
24	22.86394945450488	25.51992170831236	28.208941367259733	23.40718746992303
25	21.823467234945756	27.221266856944766	26.040077644367333	24.91518826374214
26	20.424233287424695	28.903022048922082	26.309567308961725	24.363177354691494
27	20.9839098316882	27.44816965924548	28.16396964067761	23.40395086838871
28	18.91504006146136	27.164285635195828	28.43499243231457	25.48568187102824
29	21.47416979041302	27.242134419468666	28.03160967266886	23.252086117449455
30	22.254361107633184	26.801445568453698	28.901063053256575	22.043130270656544
31	24.047268010196998	26.958335569143603	26.440990366000012	22.553406054659387
32	26.031645445633185	27.119654603512057	26.03888521222311	20.809814738631648
33	25.46600674064856	25.798269440263834	26.347214095229333	22.38850972385827
34	21.734886561374907	25.448546127108155	30.028763166793148	22.787804144723786
35	22.348137378403862	27.538198286134314	27.34604636346524	22.767617971996582
36	23.71662361134889	28.63855763407835	25.97560113485471	21.669217619718058
37	22.371474978940796	27.838180144130973	27.38761114106387	22.402733735864356
38	23.897617776097015	26.427362570066038	26.147055842449053	23.5279638113879
39	23.818832080853714	23.30148687771012	28.64664913791415	24.23303190352202
40	24.64016230704957	23.719008475637334	29.90151362225964	21.73931559505345
41	21.47187009984916	25.118838639230507	27.78460587136553	25.62468538955481
42	24.279877452045064	25.102400110385148	25.94672724221959	24.670995195350194
43	23.688345934785886	24.18984882515623	27.225951411797073	24.89585382826081
44	23.17210799006193	24.365732566429116	27.082178164693616	25.379981278815333
45	22.22574273617183	23.843532460984047	27.14529189461285	26.78543290823127
46	21.57705964971454	25.214659079391282	28.66402457772997	24.54425669316421
47	19.845988871201143	26.20258911088001	30.54866358167436	23.402758436244486
48	21.538901821099405	25.32512940018108	28.875255414706608	24.260713364012908
49	23.55002380605602	22.959003331144366	30.030807336183248	23.460165526616365
50	21.635829519679813	24.008769486683516	28.38950966338492	25.96589133025175
51	20.70079237115984	24.02009759205363	26.71533493289588	28.56377510389065
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	313.0
1	715.5
2	1118.0
3	1712.5
4	2307.0
5	1760.0
6	1213.0
7	1306.5
8	1400.0
9	1579.5
10	1759.0
11	1982.5
12	2206.0
13	2311.5
14	2417.0
15	2351.5
16	2286.0
17	2443.0
18	2600.0
19	2517.0
20	2434.0
21	3384.5
22	4335.0
23	4175.0
24	4015.0
25	5144.5
26	8278.0
27	10282.0
28	11340.5
29	12399.0
30	14711.0
31	17023.0
32	19820.5
33	22618.0
34	26579.5
35	30541.0
36	32667.0
37	34793.0
38	39029.5
39	43266.0
40	48519.5
41	53773.0
42	61752.5
43	69732.0
44	74458.0
45	79184.0
46	91543.5
47	103903.0
48	118110.5
49	132318.0
50	128217.5
51	124117.0
52	106043.0
53	87969.0
54	79412.0
55	70855.0
56	65171.0
57	59487.0
58	57013.0
59	54539.0
60	47303.5
61	40068.0
62	34749.5
63	29431.0
64	25836.0
65	22241.0
66	18701.0
67	15161.0
68	12304.0
69	9447.0
70	7682.5
71	5918.0
72	5213.0
73	4508.0
74	3675.5
75	2110.0
76	1377.0
77	1134.5
78	892.0
79	671.5
80	451.0
81	307.5
82	164.0
83	100.5
84	37.0
85	25.0
86	13.0
87	28.0
88	43.0
89	21.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1174071.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.401273696673286
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.22084551670706	17.328963687883427
2	12.242056696525085	6.219276655172514
3	5.400582639595486	4.1154503324960166
4	2.953829254400821	3.001241013771026
5	1.8900285098141976	2.4004565736153
6	1.2505565422020704	1.9059437400984092
7	0.9364552239205614	1.6651008813239954
8	0.7148469242671789	1.4526417899628556
9	0.5521853332655434	1.262358970341014
>10	4.174316238815363	24.44059093995077
>50	1.4652942505109663	25.498618661439565
>100	0.1892544185749226	7.443806011270417
>500	0.007059223428161707	1.2464833631828127
>1k	0.0020169209794747736	1.0797220254450381
>5k	6.723069931582579E-4	0.9393453540467271
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC	5679	0.4837015819315868	No Hit
CTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGCT	5322	0.4532945622539012	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC	3864	0.3291112718055382	No Hit
TCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC	3864	0.3291112718055382	No Hit
TCCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTG	1627	0.13857764990362595	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.51737245873546E-5	0.0	0.0	0.46717787936163996	0.0
2	8.51737245873546E-5	0.0	0.0	1.6784334167184096	0.0
3	8.51737245873546E-5	0.0	0.0	2.3365707866048986	0.0
4	8.51737245873546E-5	0.0	0.0	3.0330363325556973	0.0
5	8.51737245873546E-5	0.0	0.0	4.286878732206144	0.0
6	8.51737245873546E-5	0.0	0.0	5.1096569117199895	0.0
7	8.51737245873546E-5	0.0	0.0	5.883034330973169	0.0
8	8.51737245873546E-5	0.0	0.0	7.136195340826918	0.0
9	8.51737245873546E-5	0.0	0.0	7.757878356590019	0.0
10	8.51737245873546E-5	0.0	0.0	8.89341445278863	0.0
11	8.51737245873546E-5	0.0	0.0	10.68708791887373	0.0
12	8.51737245873546E-5	0.0	0.0	12.238953180855331	0.0
13	8.51737245873546E-5	0.0	0.0	12.763708498038023	0.0
14	8.51737245873546E-5	0.0	0.0	12.960715323008575	0.0
15	8.51737245873546E-5	0.0	0.0	13.272962197345816	0.0
16	8.51737245873546E-5	0.0	0.0	13.976667509886541	0.0
17	8.51737245873546E-5	0.0	0.0	14.948244186254493	0.0
18	8.51737245873546E-5	0.0	0.0	16.043237589549523	0.0
19	8.51737245873546E-5	0.0	0.0	16.77334675671233	0.0
20	8.51737245873546E-5	0.0	0.0	17.54289135835908	0.0
21	8.51737245873546E-5	0.0	0.0	18.54674887634564	0.0
22	8.51737245873546E-5	0.0	0.0	19.503420150910806	0.0
23	8.51737245873546E-5	0.0	0.0	20.38641615370791	0.0
24	8.51737245873546E-5	0.0	0.0	21.10502686805142	0.0
25	2.555211737620638E-4	0.0	0.0	21.72900957437838	0.0
26	2.555211737620638E-4	0.0	0.0	22.336724099309155	0.0
27	2.555211737620638E-4	0.0	0.0	22.93438812473862	0.0
28	2.555211737620638E-4	0.0	0.0	23.533840798384425	0.0
29	2.555211737620638E-4	0.0	0.0	24.199984498382126	0.0
30	2.555211737620638E-4	0.0	0.0	24.921661466810782	0.0
31	3.406948983494184E-4	0.0	0.0	25.53857475399699	0.0
32	3.406948983494184E-4	0.0	0.0	26.13640912687563	0.0
33	3.406948983494184E-4	0.0	0.0	26.735265584449323	0.0
34	3.406948983494184E-4	0.0	0.0	27.33190752518374	0.0
35	3.406948983494184E-4	0.0	0.0	27.986297251188386	0.0
36	3.406948983494184E-4	0.0	0.0	28.568289311293782	0.0
37	3.406948983494184E-4	0.0	0.0	29.173618971936108	0.0
38	3.406948983494184E-4	0.0	0.0	29.836951939022427	0.0
39	3.406948983494184E-4	0.0	0.0	30.801544369974216	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATTG	30	2.1655414E-6	45.000004	12
ATTAGCG	20	7.0333487E-4	45.0	14
ACGCATT	20	7.0333487E-4	45.0	10
CACGCAT	25	3.890857E-5	45.0	36
CGCGATT	20	7.0333487E-4	45.0	13
TAACGGT	20	7.0333487E-4	45.0	24
ACGGGTA	45	3.8562575E-10	45.0	5
ACCTCGC	20	7.0333487E-4	45.0	33
GTTACCG	20	7.0333487E-4	45.0	38
TACGCTT	20	7.0333487E-4	45.0	15
CGTAGTA	35	1.2119563E-7	45.0	21
CGAATCT	20	7.0333487E-4	45.0	27
CGTAAGT	25	3.890857E-5	45.0	30
TTAAACG	20	7.0333487E-4	45.0	31
TTACGCC	20	7.0333487E-4	45.0	14
TTACGAG	20	7.0333487E-4	45.0	25
CGAGACG	20	7.0333487E-4	45.0	14
GACGCGT	20	7.0333487E-4	45.0	32
CCGAAAT	20	7.0333487E-4	45.0	45
GCTACGC	20	7.0333487E-4	45.0	17
>>END_MODULE