SRR2935309.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935309.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	857352
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC	5649	0.6588892310276292	TruSeq Adapter, Index 19 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGCT	5278	0.6156164562513413	TruSeq Adapter, Index 13 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC	4894	0.5708273847847791	TruSeq Adapter, Index 19 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC	4013	0.4680691244669634	TruSeq Adapter, Index 19 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTG	1946	0.2269779507133593	TruSeq Adapter, Index 13 (95% over 22bp)
TTCCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCT	1354	0.15792813220240928	TruSeq Adapter, Index 19 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTG	1333	0.15547872985658165	TruSeq Adapter, Index 13 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTG	1268	0.14789724640521046	TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCC	1038	0.12107045880805083	No Hit
GTTGTCGGGATCTAAAAAGAGCCCTCCTTGTGCTTGTTCTTGGGTGTGATG	1024	0.11943752391083241	No Hit
TGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGCTT	972	0.11337233714973546	TruSeq Adapter, Index 19 (96% over 25bp)
GTCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTG	965	0.11255586970112626	TruSeq Adapter, Index 19 (95% over 22bp)
TTCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTG	895	0.10439119521503419	TruSeq Adapter, Index 13 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTC	90	0.0	45.000004	6
CGGAATC	35	1.211447E-7	45.000004	27
CTAGACG	35	1.211447E-7	45.000004	32
GGCGTAG	35	1.211447E-7	45.000004	1
TGACCTA	45	3.8380676E-10	45.000004	30
GGTATAC	35	1.211447E-7	45.000004	8
TCGAATA	35	1.211447E-7	45.000004	19
AATGTCG	35	1.211447E-7	45.000004	24
CCCGAGT	35	1.211447E-7	45.000004	44
GTTGACG	50	2.1827873E-11	45.0	1
ACACGTA	20	7.032283E-4	45.0	13
ACACGAT	20	7.032283E-4	45.0	16
CGAGTTT	20	7.032283E-4	45.0	27
CGAAACA	20	7.032283E-4	45.0	12
TTAGCGT	20	7.032283E-4	45.0	41
GACGTAA	20	7.032283E-4	45.0	12
CGTTGCA	20	7.032283E-4	45.0	29
ATTACGT	25	3.8899736E-5	45.0	13
GACCGAA	20	7.032283E-4	45.0	18
CGACCTA	25	3.8899736E-5	45.0	11