##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935308.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1094518
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2781078063586	33.0	31.0	34.0	30.0	34.0
2	32.4314355725534	34.0	31.0	34.0	30.0	34.0
3	32.48198110949295	34.0	31.0	34.0	30.0	34.0
4	35.95383630054508	37.0	35.0	37.0	35.0	37.0
5	31.927273009671836	37.0	35.0	37.0	0.0	37.0
6	33.511339237911116	37.0	35.0	37.0	17.0	37.0
7	35.250180444725444	37.0	35.0	37.0	32.0	37.0
8	35.76664431283908	37.0	35.0	37.0	35.0	37.0
9	37.8109898603769	39.0	38.0	39.0	35.0	39.0
10	37.54528568739847	39.0	37.0	39.0	35.0	39.0
11	37.41322481676866	39.0	37.0	39.0	35.0	39.0
12	37.118281289115394	39.0	37.0	39.0	34.0	39.0
13	36.99720242152253	39.0	37.0	39.0	33.0	39.0
14	38.12900564449374	40.0	37.0	41.0	33.0	41.0
15	38.24317736208998	40.0	37.0	41.0	34.0	41.0
16	38.34456171575068	40.0	37.0	41.0	34.0	41.0
17	38.33339698387783	40.0	37.0	41.0	34.0	41.0
18	38.32828514469383	40.0	37.0	41.0	34.0	41.0
19	38.355259575447825	40.0	37.0	41.0	34.0	41.0
20	38.26807690691245	40.0	37.0	41.0	34.0	41.0
21	38.21152964135811	40.0	37.0	41.0	34.0	41.0
22	38.18940300662027	40.0	37.0	41.0	34.0	41.0
23	38.13890680646641	40.0	37.0	41.0	34.0	41.0
24	38.11094015813354	40.0	36.0	41.0	34.0	41.0
25	38.01990922031433	40.0	36.0	41.0	34.0	41.0
26	37.93791970529493	40.0	36.0	41.0	34.0	41.0
27	37.89611226128762	40.0	36.0	41.0	34.0	41.0
28	37.86356277375064	40.0	36.0	41.0	34.0	41.0
29	37.8097007084397	40.0	36.0	41.0	34.0	41.0
30	37.781155723341236	40.0	36.0	41.0	34.0	41.0
31	37.567545714186515	39.0	36.0	41.0	33.0	41.0
32	37.59109580655595	39.0	35.0	41.0	33.0	41.0
33	37.622399997076336	39.0	35.0	41.0	33.0	41.0
34	37.574112988548386	40.0	35.0	41.0	33.0	41.0
35	37.52058988522802	40.0	35.0	41.0	33.0	41.0
36	37.485664922824476	39.0	35.0	41.0	33.0	41.0
37	37.447719452763685	39.0	35.0	41.0	33.0	41.0
38	37.429527883506715	39.0	35.0	41.0	33.0	41.0
39	37.446429387182306	39.0	35.0	41.0	33.0	41.0
40	37.40772924702929	39.0	35.0	41.0	33.0	41.0
41	37.351481656765806	39.0	35.0	41.0	33.0	41.0
42	37.32844868700195	39.0	35.0	41.0	33.0	41.0
43	37.27276938341809	39.0	35.0	41.0	33.0	41.0
44	37.20220133428596	39.0	35.0	41.0	33.0	41.0
45	37.18695352657517	39.0	35.0	41.0	33.0	41.0
46	37.07611021472466	39.0	35.0	41.0	33.0	41.0
47	37.00153583586565	39.0	35.0	41.0	32.0	41.0
48	36.989529637703534	39.0	35.0	41.0	32.0	41.0
49	36.99739611408857	39.0	35.0	41.0	33.0	41.0
50	36.93171057945141	39.0	35.0	41.0	32.0	41.0
51	36.29557485578127	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	12.0
11	16.0
12	12.0
13	18.0
14	22.0
15	24.0
16	46.0
17	70.0
18	104.0
19	210.0
20	376.0
21	652.0
22	1157.0
23	1746.0
24	2420.0
25	3583.0
26	4889.0
27	6174.0
28	7734.0
29	10063.0
30	13836.0
31	18625.0
32	26073.0
33	40096.0
34	69889.0
35	103473.0
36	89062.0
37	139540.0
38	229634.0
39	324623.0
40	331.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.23817424656333	4.400384461470711	55.65134607196958	16.710095219996383
2	19.09379288417367	5.624393568675893	52.74641440341776	22.535399143732675
3	19.71607593479504	5.397992541008919	51.86118455795153	23.024746966244503
4	19.4494745632324	3.986412283763264	51.93966659296604	24.624446560038297
5	16.49538883782633	15.99142270844335	43.93075308035135	23.582435373378967
6	21.037479511529273	7.407735642538542	51.86319457514632	19.69159027078586
7	80.5962990101579	2.2033443031544477	11.323797324484385	5.876559362203271
8	81.44361262217707	3.173177599637466	8.559658223985354	6.8235515542001135
9	76.09550505336595	5.075384781246174	11.373225474592468	7.455884690795401
10	41.130159577092385	24.639704417835066	18.78699116871536	15.443144836357192
11	29.995121140081753	24.5424926771419	24.324040353836118	21.138345828940228
12	25.98678139601176	21.90059916785288	30.905476200482767	21.20714323565259
13	22.763992917430322	26.311764630641065	31.32127566654911	19.6029667853795
14	18.625276148953237	28.345445209672203	30.486113522116586	22.543165119257974
15	16.156975033759153	25.050022018824723	36.86892312415145	21.92407982326467
16	18.730984780515257	24.453138276391982	32.04972417082222	24.766152772270534
17	18.243646975198217	24.89470250831873	29.25022704057859	27.611423475904463
18	19.902276618566347	23.78672621190332	32.13103850279301	24.17995866673732
19	22.038011252441713	24.98442236674043	29.319389904962733	23.658176475855125
20	23.51875437407151	25.858140295545617	30.012389015073303	20.61071631530957
21	23.424374930334633	26.473936472492916	29.472151211766274	20.62953738540618
22	21.861129739300768	24.52577298865802	28.442382857111532	25.17071441492968
23	20.26937884986816	25.510133227594245	27.76930119011291	26.451186732424688
24	21.647885187817835	23.894079402988346	29.55556692534979	24.902468483844032
25	18.9450516117597	27.194344907986896	27.540707416415263	26.319896063838144
26	18.57502571908365	29.143056578329457	27.434907420435295	24.847010282151597
27	20.952419238422756	26.577178264770428	29.47004983015355	23.000352666653267
28	17.62648033198175	25.216487988319976	32.957246934266955	24.199784745431323
29	18.507781507476352	23.862010492289755	33.379441909589424	24.250766090644465
30	21.885158581220228	24.217326713676705	29.865018208928497	24.03249649617457
31	23.743419477797534	26.13844632979997	26.69430744857554	23.423826743826965
32	21.050727352131258	26.33223026026068	27.185939381535984	25.43110300607208
33	22.53804871185307	23.48056404737062	27.617910349578533	26.363476891197767
34	19.944852437328578	23.880192011460753	30.075978649962813	26.098976901247855
35	19.110421208239607	24.88501788001659	28.413968523130727	27.59059238861307
36	22.757871501428024	25.21502615763286	28.77769027096859	23.249412069970525
37	21.685344599175163	23.80481636665637	32.893657299377445	21.616181734791024
38	18.67351656162804	25.11927624762681	30.724757381788148	25.482449808957004
39	20.42259697876143	23.628208946769263	31.373536113613483	24.57565796085583
40	23.135754734047314	22.320692761562626	27.970120180755366	26.573432323634695
41	20.49651079287869	23.21725179485399	26.927652172006304	29.35858524026101
42	20.350967275092778	22.53804871185307	29.447300090085314	27.663683922968836
43	21.836644075291588	21.784840450316942	31.1663216137149	25.212193860676575
44	19.76577817815696	22.556778417531735	30.41384426752233	27.26359913678898
45	21.379365163478354	23.22309911760245	27.644680124036334	27.752855594882863
46	23.654887356809116	23.204460776341733	28.946623079748345	24.194028787100805
47	18.642178566273007	23.241372001191394	32.7098320904727	25.406617342062898
48	18.973557310158444	21.601837521173703	33.18529252145693	26.239312647210916
49	21.042230461262402	19.34413138934216	34.70413460536967	24.90950354402577
50	21.443959806965257	19.659429995669324	31.18806634518573	27.70854385217968
51	19.71178180715164	20.063260722984914	28.591032765107567	31.633924704755884
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	417.0
1	705.5
2	994.0
3	1540.5
4	2087.0
5	1663.0
6	1239.0
7	1267.5
8	1296.0
9	1673.0
10	2050.0
11	2195.5
12	2341.0
13	2499.0
14	2657.0
15	2485.0
16	2313.0
17	2441.5
18	2570.0
19	2459.5
20	2349.0
21	3125.0
22	3901.0
23	3815.0
24	3729.0
25	4426.5
26	6982.0
27	8840.0
28	9838.0
29	10836.0
30	12554.5
31	14273.0
32	16338.0
33	18403.0
34	21565.0
35	24727.0
36	26339.0
37	27951.0
38	31772.5
39	35594.0
40	42885.5
41	50177.0
42	57996.5
43	65816.0
44	76789.5
45	87763.0
46	103501.0
47	119239.0
48	132964.5
49	146690.0
50	138114.5
51	129539.0
52	107076.5
53	84614.0
54	71754.0
55	58894.0
56	52243.0
57	45592.0
58	39858.0
59	34124.0
60	31378.0
61	28632.0
62	25209.0
63	21786.0
64	19334.0
65	16882.0
66	13957.5
67	11033.0
68	9082.5
69	7132.0
70	6104.0
71	5076.0
72	3971.5
73	2867.0
74	2580.5
75	1849.0
76	1404.0
77	1107.0
78	810.0
79	527.0
80	244.0
81	175.5
82	107.0
83	103.0
84	99.0
85	52.5
86	6.0
87	4.5
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1094518.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.776312746967946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.900287882385435	15.832135171952674
2	13.522667519553908	6.700836792754658
3	6.282710918010771	4.669872318102749
4	3.5873572921375105	3.5552594482046014
5	2.340338419601272	2.899247830889291
6	1.6361559874214573	2.432274746830691
7	1.191323052888791	2.066161477873173
8	0.9327818820585289	1.8488716507669984
9	0.6920265466389681	1.5431279533858149
>10	4.437726462459148	23.964241993291964
>50	1.2896393437361435	21.77164671323864
>100	0.17254429106611247	7.006890331963725
>500	0.00851613453759284	1.408827227744892
>1k	0.004443200628309308	1.7469751187045635
>5k	0.0014810668761031027	2.553631224295554
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC	9300	0.8496890868857342	No Hit
CTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGCT	7180	0.6559965208429647	No Hit
TCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC	6196	0.5660939335853773	No Hit
GCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC	5160	0.4714403965946654	No Hit
TCCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTG	2444	0.22329463745685316	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGC	2316	0.21159999195993123	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCG	2311	0.2111431698702077	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTC	1741	0.15906545164172722	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTG	1593	0.14554351778591124	No Hit
TTCCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCT	1514	0.13832572876827975	No Hit
GCCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTG	1455	0.13293522810954228	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCC	1303	0.11904783658194748	No Hit
TGCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTG	1135	0.10369861436723746	No Hit
TGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGCTT	1131	0.10333315669545863	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.827288358894052E-4	0.0	0.0	0.6728075737447899	0.0
2	1.827288358894052E-4	0.0	0.0	2.6673841818955926	0.0
3	1.827288358894052E-4	0.0	0.0	3.6753164406615513	0.0
4	1.827288358894052E-4	0.0	0.0	4.754604309842323	0.0
5	1.827288358894052E-4	0.0	0.0	6.636985412757031	0.0
6	1.827288358894052E-4	0.0	0.0	7.758849100700034	0.0
7	1.827288358894052E-4	0.0	0.0	8.779389649142363	0.0
8	1.827288358894052E-4	0.0	0.0	10.279319298540544	0.0
9	1.827288358894052E-4	0.0	0.0	10.91704293579457	0.0
10	1.827288358894052E-4	0.0	0.0	12.399339252529424	0.0
11	1.827288358894052E-4	0.0	0.0	15.075768511801542	0.0
12	1.827288358894052E-4	0.0	0.0	17.528720404780916	0.0
13	1.827288358894052E-4	0.0	0.0	18.26319896063838	0.0
14	1.827288358894052E-4	0.0	0.0	18.52057252598861	0.0
15	1.827288358894052E-4	0.0	0.0	18.981414650101687	0.0
16	1.827288358894052E-4	0.0	0.0	19.9884332646882	0.0
17	1.827288358894052E-4	0.0	0.0	21.40549538701054	0.0
18	1.827288358894052E-4	0.0	0.0	22.959512771831985	0.0
19	1.827288358894052E-4	0.0	0.0	24.02856782620295	0.0
20	1.827288358894052E-4	0.0	0.0	25.07889317489525	0.0
21	1.827288358894052E-4	0.0	0.0	26.39417533562719	0.0
22	1.827288358894052E-4	0.0	0.0	27.66788668619429	0.0
23	1.827288358894052E-4	0.0	0.0	28.95256176691475	0.0
24	1.827288358894052E-4	0.0	0.0	29.9167304694852	0.0
25	1.827288358894052E-4	0.0	0.0	30.7704395907605	0.0
26	2.740932538341078E-4	0.0	0.0	31.533697938270546	0.0
27	2.740932538341078E-4	0.0	0.0	32.285992555627224	0.0
28	2.740932538341078E-4	0.0	0.0	33.076112042013015	0.0
29	2.740932538341078E-4	0.0	0.0	33.90634050787653	0.0
30	2.740932538341078E-4	0.0	0.0	34.833872078851144	0.0
31	2.740932538341078E-4	0.0	0.0	35.6237174719831	0.0
32	2.740932538341078E-4	0.0	0.0	36.373179792383496	0.0
33	2.740932538341078E-4	0.0	0.0	37.141920004970224	0.0
34	2.740932538341078E-4	0.0	0.0	37.875210823394404	0.0
35	2.740932538341078E-4	0.0	0.0	38.66286347049569	0.0
36	2.740932538341078E-4	0.0	0.0	39.37998278694366	0.0
37	2.740932538341078E-4	0.0	0.0	40.10559899426049	0.0
38	2.740932538341078E-4	0.0	0.0	40.82984473530814	0.0
39	2.740932538341078E-4	0.0	0.0	41.55189773032513	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTG	20	7.0331374E-4	45.000004	15
CGGCGTA	20	7.0331374E-4	45.000004	24
GGTACGC	20	7.0331374E-4	45.000004	8
CGAACGT	20	7.0331374E-4	45.000004	13
TCCGCTA	40	6.8139343E-9	45.000004	25
CAACCGT	20	7.0331374E-4	45.000004	14
TATGCGA	20	7.0331374E-4	45.000004	10
ACCTCGC	20	7.0331374E-4	45.000004	35
TATTGCG	40	6.8139343E-9	45.000004	1
TACGCAA	20	7.0331374E-4	45.000004	41
GCGATAA	20	7.0331374E-4	45.000004	8
CGTAAAC	35	1.2118653E-7	45.000004	28
ATGCGAA	20	7.0331374E-4	45.000004	11
ACGTAAA	35	1.2118653E-7	45.000004	27
CGCAATA	20	7.0331374E-4	45.000004	27
TGTACGC	20	7.0331374E-4	45.000004	18
TAGTCGA	20	7.0331374E-4	45.000004	42
GTAACAC	20	7.0331374E-4	45.000004	42
TCGAACG	20	7.0331374E-4	45.000004	12
ACGACCG	35	1.2118653E-7	45.000004	21
>>END_MODULE