SRR2935307.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935307.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1117623
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	5260	0.47064171012944434	RNA PCR Primer, Index 30 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGCT	4566	0.40854563658765075	RNA PCR Primer, Index 30 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	4026	0.3602288070306356	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	3118	0.2789849528866174	Illumina Single End Adapter 2 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTG	1658	0.14835056186209483	RNA PCR Primer, Index 30 (95% over 23bp)
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	1646	0.1472768545386056	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCTA	30	2.1654469E-6	45.000004	15
TGGATCG	30	2.1654469E-6	45.000004	33
AATCGTC	55	1.8189894E-12	45.000004	14
TGATCGC	25	3.8907372E-5	45.0	35
CGAACTT	25	3.8907372E-5	45.0	19
TAGCGCC	20	7.0332026E-4	45.0	21
TAGCGAA	20	7.0332026E-4	45.0	43
GACCGAA	20	7.0332026E-4	45.0	9
GCACGTT	20	7.0332026E-4	45.0	36
CGCGAAT	25	3.8907372E-5	45.0	8
CGCGAAC	20	7.0332026E-4	45.0	22
TAACGCC	20	7.0332026E-4	45.0	12
CGTTATG	20	7.0332026E-4	45.0	1
TACGCCG	20	7.0332026E-4	45.0	31
CGGATGT	20	7.0332026E-4	45.0	15
TGCGATG	25	3.8907372E-5	45.0	1
TGATTCG	25	3.8907372E-5	45.0	28
TGCGACG	20	7.0332026E-4	45.0	32
AGCGAAT	25	3.8907372E-5	45.0	40
CGTGTTA	20	7.0332026E-4	45.0	15