FastQCFastQC Report
Sat 14 Jan 2017
SRR2935306.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935306.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557113
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC56681.017387854887608Illumina PCR Primer Index 7 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGCT46580.8360960882262665Illumina PCR Primer Index 7 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC42270.7587329679975158Illumina PCR Primer Index 7 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC25600.45951180460696484Illumina PCR Primer Index 7 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTG15630.280553496328393Illumina PCR Primer Index 7 (95% over 22bp)
TTCCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCT9900.17770183068784967Illumina PCR Primer Index 7 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTG9410.1689064875527945Illumina PCR Primer Index 7 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTG7860.14108448375823218Illumina PCR Primer Index 7 (95% over 22bp)
TTCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTG7560.1356995797979943Illumina PCR Primer Index 7 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCC7240.12995568224040724No Hit
TGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTG7190.12905819824703427Illumina PCR Primer Index 7 (95% over 22bp)
TGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGCTT7150.12834021105233587Illumina PCR Primer Index 7 (96% over 25bp)
CGTTCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTC5850.10500562722463845No Hit
TTTCCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTC5630.10105669765379734No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGG351.2104283E-745.0000042
CAACGAT351.2104283E-745.00000426
CGTGATT351.2104283E-745.00000429
TTCACGG700.045.0000042
TGCATGG2050.045.0000042
ATGCGTG351.2104283E-745.00000411
TCGAATT850.045.00000418
GCCCCGT253.8882095E-545.011
CTGCTCG900.045.01
TTCTACG453.8380676E-1045.01
GAGTGAT253.8882095E-545.028
CGGCGAG207.030155E-445.019
CTCACGG207.030155E-445.02
TCGCAGG253.8882095E-545.02
TACCCGA207.030155E-445.040
GCCCACT207.030155E-445.024
CGAACGA207.030155E-445.022
CGAACCC207.030155E-445.036
GATCGTC453.8380676E-1045.020
GTACACG207.030155E-445.045
Produced by FastQC (version 0.11.5)