##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935306.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	557113
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22924254145927	33.0	31.0	34.0	30.0	34.0
2	32.39694460549296	34.0	31.0	34.0	30.0	34.0
3	32.4333016820645	34.0	31.0	34.0	30.0	34.0
4	35.910459816949164	37.0	35.0	37.0	35.0	37.0
5	31.777073950886084	37.0	35.0	37.0	0.0	37.0
6	33.5184047042521	37.0	35.0	37.0	17.0	37.0
7	35.25374385447836	37.0	35.0	37.0	32.0	37.0
8	35.799392582833285	37.0	35.0	37.0	35.0	37.0
9	37.84977912918923	39.0	38.0	39.0	35.0	39.0
10	37.507446424693015	39.0	37.0	39.0	35.0	39.0
11	37.39110557463208	39.0	37.0	39.0	35.0	39.0
12	37.00378020258009	39.0	35.0	39.0	34.0	39.0
13	36.76877222394739	39.0	35.0	39.0	33.0	39.0
14	37.805448804820564	40.0	36.0	41.0	33.0	41.0
15	37.97074740672	40.0	36.0	41.0	33.0	41.0
16	38.0737803641272	40.0	36.0	41.0	34.0	41.0
17	38.11381174016761	40.0	36.0	41.0	34.0	41.0
18	38.14951544839198	40.0	36.0	41.0	34.0	41.0
19	38.16190611240449	40.0	36.0	41.0	34.0	41.0
20	38.06416292565422	40.0	36.0	41.0	34.0	41.0
21	37.99881352616076	40.0	36.0	41.0	34.0	41.0
22	37.95328416317695	40.0	36.0	41.0	34.0	41.0
23	37.95601969438875	40.0	35.0	41.0	34.0	41.0
24	37.93824053648003	40.0	35.0	41.0	34.0	41.0
25	37.84229231771651	39.0	35.0	41.0	34.0	41.0
26	37.76994613301072	39.0	35.0	41.0	34.0	41.0
27	37.66393173377753	39.0	35.0	41.0	33.0	41.0
28	37.647349819515966	39.0	35.0	41.0	33.0	41.0
29	37.480015723919564	39.0	35.0	41.0	33.0	41.0
30	37.456027771744694	39.0	35.0	41.0	33.0	41.0
31	37.22626289460128	39.0	35.0	41.0	33.0	41.0
32	37.22894816670944	39.0	35.0	41.0	33.0	41.0
33	37.245128008141975	39.0	35.0	41.0	33.0	41.0
34	37.27862031580667	39.0	35.0	41.0	33.0	41.0
35	37.18258055367583	39.0	35.0	41.0	32.0	41.0
36	37.16306566172392	39.0	35.0	41.0	32.0	41.0
37	37.09317858315997	39.0	35.0	41.0	32.0	41.0
38	37.00880431797499	39.0	35.0	41.0	32.0	41.0
39	37.03042829731132	39.0	35.0	41.0	32.0	41.0
40	37.05867032361478	39.0	35.0	41.0	32.0	41.0
41	37.098455789041004	39.0	35.0	41.0	32.0	41.0
42	37.12319044789836	39.0	35.0	41.0	32.0	41.0
43	36.98562589636214	39.0	35.0	41.0	32.0	41.0
44	36.84717463064046	39.0	35.0	41.0	32.0	41.0
45	36.74343266087849	39.0	35.0	40.0	31.0	41.0
46	36.64499482151736	38.0	35.0	40.0	31.0	41.0
47	36.55133877687292	38.0	35.0	40.0	31.0	41.0
48	36.44025718301314	38.0	35.0	40.0	31.0	41.0
49	36.38004857183372	38.0	35.0	40.0	31.0	41.0
50	36.230145410356606	37.0	35.0	40.0	31.0	41.0
51	35.51630459170761	36.0	34.0	39.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	0.0
12	6.0
13	4.0
14	8.0
15	14.0
16	25.0
17	28.0
18	64.0
19	89.0
20	162.0
21	302.0
22	468.0
23	770.0
24	1175.0
25	1784.0
26	2406.0
27	3283.0
28	4271.0
29	5810.0
30	7710.0
31	10707.0
32	15055.0
33	24131.0
34	41152.0
35	64256.0
36	47488.0
37	73800.0
38	119067.0
39	132951.0
40	122.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.31060126042652	4.263407962118996	59.092859078858325	16.333131698596155
2	18.9541439528426	3.818614895003348	55.65064897067561	21.576592181478443
3	20.004559218686335	5.294437573705872	53.23749400929434	21.46350919831345
4	18.958272379212115	3.66873506811006	53.543177057437184	23.82981549524064
5	14.936646604907802	16.51424396845882	46.68675834166498	21.8623510849684
6	19.359088730652488	5.404648608092074	55.074643743728835	20.161618917526607
7	80.64773214769716	1.8244054617285903	11.222139853135719	6.305722537438545
8	82.48829232130645	1.6578324325585654	8.603820050869393	7.250055195265592
9	78.10336502648475	4.138478190241477	11.437177017947885	6.320979765325885
10	38.82426006932167	24.94161866623109	20.067024104625094	16.167097159822156
11	31.791216503653658	25.773945321685186	24.807893551218513	17.626944623442643
12	28.200742039765718	21.69936799177187	30.662899627185148	19.436990341277262
13	22.039873418857574	29.87203673222488	29.41037096603382	18.677718882883724
14	16.497191772584735	33.16993141427322	29.98099128902036	20.351885524121677
15	14.641912861484116	25.400232986844678	40.646332072667484	19.31152207900372
16	14.922286861013834	24.16762847034623	37.49616325592833	23.413921412711606
17	15.211994694074631	23.841662283953166	29.57712349200252	31.369219529969683
18	17.905703151784287	22.454511023795888	35.51039017219128	24.129395652228542
19	21.473022528643202	24.988467330685157	30.51140432910379	23.02710581156785
20	24.543494766770834	26.14263174616281	29.137535832048435	20.176337655017925
21	20.1086673619176	28.506066094311205	30.07774006350597	21.307526480265224
22	18.502171013779968	26.374182616453034	28.122481435543595	27.001164934223397
23	17.100121519332703	27.482216354671312	27.239357186064588	28.178304939931397
24	20.75036841717928	23.9805928061273	31.489661881880338	23.77937689481308
25	18.322135724709348	26.055396302006955	29.171640223796608	26.45082774948709
26	19.320137925340102	28.452037557910153	28.23035901154703	23.997465505202715
27	22.257064545253836	27.997192670068728	29.017452473735133	20.728290310942306
28	19.676080077111823	25.276021202161857	33.287322320606414	21.760576400119906
29	19.480069572959167	22.977385198334986	32.883275026789896	24.659270201915948
30	22.659137374284928	26.13922130698799	28.401239963885246	22.80040135484184
31	24.668963029044377	29.013503544164287	24.55695702667143	21.760576400119906
32	23.24824586753495	27.641609511894355	27.42118744312195	21.68895717744874
33	22.900381071703585	28.15622683369442	25.79207449835132	23.15131759625067
34	20.59025727276154	25.969955825837847	28.905805464959535	24.53398143644108
35	20.375938095144072	23.633804991087985	29.138792309639154	26.851464604128783
36	27.185508146462205	24.948080550983374	26.82346310353555	21.04294819901887
37	22.340710053436197	27.593504369849565	29.91565445430281	20.150131122411434
38	18.776621618953428	28.543042434838174	28.800081850540195	23.880254095668203
39	18.614536009750264	26.68255811657599	31.139463627666203	23.563442246007543
40	22.44194624788867	24.7373513093394	28.249026678609184	24.571675764162745
41	18.25931184517324	25.42087511869226	28.66312579315148	27.65668724298302
42	23.75909375656285	24.428975809216443	27.264666234677705	24.547264199543
43	25.072471832464867	23.45161574043327	28.51001502388205	22.965897403219813
44	21.44879046082213	23.08059585757288	29.438192969828382	26.032420711776606
45	21.1296451527787	24.41802650449729	26.570193120605694	27.882135222118315
46	23.273734412946744	24.272454600772196	29.465655980025595	22.988155006255464
47	19.750212254964435	25.177477459689506	31.735931489661883	23.33637879568418
48	20.48237969675811	22.75678363276391	32.45104673558147	24.30978993489651
49	21.79019337190121	20.760958728301084	32.88614697556869	24.562700924229016
50	21.25726737663634	22.080798688955383	30.00791580882155	26.65401812558673
51	18.26038882596529	22.85640435602831	27.283692895337214	31.599513922669185
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	169.0
1	238.0
2	307.0
3	506.5
4	706.0
5	544.5
6	383.0
7	496.0
8	609.0
9	736.5
10	864.0
11	1013.0
12	1162.0
13	1184.5
14	1207.0
15	1264.5
16	1322.0
17	1202.0
18	1082.0
19	1134.0
20	1186.0
21	1412.0
22	1638.0
23	1914.0
24	2190.0
25	2613.0
26	3594.5
27	4153.0
28	4928.0
29	5703.0
30	6551.5
31	7400.0
32	7641.0
33	7882.0
34	9298.5
35	10715.0
36	12815.0
37	14915.0
38	16738.5
39	18562.0
40	24319.0
41	30076.0
42	36455.0
43	42834.0
44	47962.5
45	53091.0
46	60802.5
47	68514.0
48	77544.5
49	86575.0
50	78702.0
51	70829.0
52	56239.0
53	41649.0
54	34000.5
55	26352.0
56	21570.0
57	16788.0
58	14249.5
59	11711.0
60	9803.0
61	7895.0
62	7038.5
63	6182.0
64	5303.5
65	4425.0
66	3086.0
67	1747.0
68	1397.0
69	1047.0
70	994.5
71	942.0
72	779.0
73	616.0
74	444.0
75	219.0
76	166.0
77	135.0
78	104.0
79	88.5
80	73.0
81	41.0
82	9.0
83	16.5
84	24.0
85	12.0
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	557113.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.0210828936388
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.94635138374415	16.639533101021602
2	13.209568136546595	6.874545349404975
3	6.279773165841472	4.902194943048284
4	3.7681420201795026	3.922045434483775
5	2.4633195061950444	3.2049120532109323
6	1.7028573709934727	2.658611568399899
7	1.248606187793886	2.2743059579866527
8	0.9330319642233138	1.9422801666775582
9	0.6833741374530633	1.6003921570231567
>10	4.5261603965867065	24.464131809398154
>50	1.0514725033502261	18.90389653641183
>100	0.17420846209352858	7.564868491871299
>500	0.00967824789408492	1.6887569648948435
>1k	0.002765213684024263	2.3399393480349513
>5k	6.913034210060657E-4	1.0195861181320807
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	5668	1.017387854887608	Illumina PCR Primer Index 7 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGCT	4658	0.8360960882262665	Illumina PCR Primer Index 7 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	4227	0.7587329679975158	Illumina PCR Primer Index 7 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	2560	0.45951180460696484	Illumina PCR Primer Index 7 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTG	1563	0.280553496328393	Illumina PCR Primer Index 7 (95% over 22bp)
TTCCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCT	990	0.17770183068784967	Illumina PCR Primer Index 7 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTG	941	0.1689064875527945	Illumina PCR Primer Index 7 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTG	786	0.14108448375823218	Illumina PCR Primer Index 7 (95% over 22bp)
TTCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTG	756	0.1356995797979943	Illumina PCR Primer Index 7 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCC	724	0.12995568224040724	No Hit
TGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTG	719	0.12905819824703427	Illumina PCR Primer Index 7 (95% over 22bp)
TGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGCTT	715	0.12834021105233587	Illumina PCR Primer Index 7 (96% over 25bp)
CGTTCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTC	585	0.10500562722463845	No Hit
TTTCCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTC	563	0.10105669765379734	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8743289063439553	0.0
2	0.0	0.0	0.0	3.3174598331038765	0.0
3	0.0	0.0	0.0	4.534089134520285	0.0
4	0.0	0.0	0.0	5.836697402501827	0.0
5	0.0	0.0	0.0	7.910244420790756	0.0
6	0.0	0.0	0.0	9.254136952467453	0.0
7	0.0	0.0	0.0	10.635364818268465	0.0
8	0.0	0.0	0.0	12.608214132500947	0.0
9	0.0	0.0	0.0	13.52580176732548	0.0
10	0.0	0.0	0.0	15.186685645461512	0.0
11	0.0	0.0	0.0	18.186795138508703	0.0
12	0.0	0.0	0.0	20.761138225099756	0.0
13	0.0	0.0	0.0	21.593823874151205	0.0
14	0.0	0.0	0.0	21.88137774562791	0.0
15	0.0	0.0	0.0	22.259398003636605	0.0
16	1.7949679867459563E-4	0.0	0.0	23.422537259047985	0.0
17	1.7949679867459563E-4	0.0	0.0	25.201889024309253	0.0
18	1.7949679867459563E-4	0.0	0.0	27.412033106389547	0.0
19	1.7949679867459563E-4	0.0	0.0	28.52886218774288	0.0
20	1.7949679867459563E-4	0.0	0.0	29.632767499591644	0.0
21	1.7949679867459563E-4	0.0	0.0	31.191697196080508	0.0
22	1.7949679867459563E-4	0.0	0.0	32.59123373534633	0.0
23	3.5899359734919127E-4	0.0	0.0	34.011591903258406	0.0
24	3.5899359734919127E-4	0.0	0.0	35.06739207306238	0.0
25	3.5899359734919127E-4	0.0	0.0	35.965235060032704	0.0
26	3.5899359734919127E-4	0.0	0.0	36.80294661944704	0.0
27	3.5899359734919127E-4	0.0	0.0	37.52811368609241	0.0
28	3.5899359734919127E-4	0.0	0.0	38.31754060666328	0.0
29	3.5899359734919127E-4	0.0	0.0	39.20515227610915	0.0
30	3.5899359734919127E-4	0.0	0.0	40.188076745651244	0.0
31	3.5899359734919127E-4	0.0	0.0	41.10243343809963	0.0
32	3.5899359734919127E-4	0.0	0.0	41.90819456734989	0.0
33	3.5899359734919127E-4	0.0	0.0	42.683261744026794	0.0
34	3.5899359734919127E-4	0.0	0.0	43.4470206223872	0.0
35	3.5899359734919127E-4	0.0	0.0	44.30322035206502	0.0
36	3.5899359734919127E-4	0.0	0.0	45.05854288088772	0.0
37	3.5899359734919127E-4	0.0	0.0	45.837199993538114	0.0
38	3.5899359734919127E-4	0.0	0.0	46.59988189110647	0.0
39	3.5899359734919127E-4	0.0	0.0	47.354486432734475	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGG	35	1.2104283E-7	45.000004	2
CAACGAT	35	1.2104283E-7	45.000004	26
CGTGATT	35	1.2104283E-7	45.000004	29
TTCACGG	70	0.0	45.000004	2
TGCATGG	205	0.0	45.000004	2
ATGCGTG	35	1.2104283E-7	45.000004	11
TCGAATT	85	0.0	45.000004	18
GCCCCGT	25	3.8882095E-5	45.0	11
CTGCTCG	90	0.0	45.0	1
TTCTACG	45	3.8380676E-10	45.0	1
GAGTGAT	25	3.8882095E-5	45.0	28
CGGCGAG	20	7.030155E-4	45.0	19
CTCACGG	20	7.030155E-4	45.0	2
TCGCAGG	25	3.8882095E-5	45.0	2
TACCCGA	20	7.030155E-4	45.0	40
GCCCACT	20	7.030155E-4	45.0	24
CGAACGA	20	7.030155E-4	45.0	22
CGAACCC	20	7.030155E-4	45.0	36
GATCGTC	45	3.8380676E-10	45.0	20
GTACACG	20	7.030155E-4	45.0	45
>>END_MODULE