##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935305.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1847838
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27810176000277	33.0	31.0	34.0	30.0	34.0
2	32.445086095209646	34.0	31.0	34.0	30.0	34.0
3	32.520329163054335	34.0	31.0	34.0	30.0	34.0
4	35.96709505919891	37.0	35.0	37.0	35.0	37.0
5	31.73679673218107	37.0	35.0	37.0	0.0	37.0
6	33.35772670547959	37.0	35.0	37.0	17.0	37.0
7	35.18643409216609	37.0	35.0	37.0	32.0	37.0
8	35.75124713313613	37.0	35.0	37.0	35.0	37.0
9	37.827487582785935	39.0	38.0	39.0	35.0	39.0
10	37.549502174974215	39.0	37.0	39.0	35.0	39.0
11	37.42713809327441	39.0	37.0	39.0	35.0	39.0
12	37.17040563079664	39.0	37.0	39.0	34.0	39.0
13	37.04794954968996	39.0	37.0	39.0	33.0	39.0
14	38.06884099147219	40.0	37.0	41.0	33.0	41.0
15	38.233547529599456	40.0	37.0	41.0	33.0	41.0
16	38.349057655487115	40.0	37.0	41.0	34.0	41.0
17	38.37808076249108	40.0	37.0	41.0	34.0	41.0
18	38.343930582659304	40.0	37.0	41.0	34.0	41.0
19	38.367875863576785	40.0	37.0	41.0	34.0	41.0
20	38.2805792499126	40.0	37.0	41.0	34.0	41.0
21	38.20638226944137	40.0	37.0	41.0	34.0	41.0
22	38.21848452082921	40.0	37.0	41.0	34.0	41.0
23	38.17011447973253	40.0	37.0	41.0	34.0	41.0
24	38.115072858118516	40.0	36.0	41.0	34.0	41.0
25	38.02607317308119	40.0	36.0	41.0	34.0	41.0
26	37.98275552294086	40.0	36.0	41.0	34.0	41.0
27	37.95080304658742	40.0	36.0	41.0	34.0	41.0
28	37.91717401633693	40.0	36.0	41.0	34.0	41.0
29	37.84866422272948	40.0	36.0	41.0	34.0	41.0
30	37.79199367044081	40.0	36.0	41.0	33.0	41.0
31	37.59297081237641	40.0	36.0	41.0	33.0	41.0
32	37.60325201668112	40.0	35.0	41.0	33.0	41.0
33	37.61424540462963	40.0	35.0	41.0	33.0	41.0
34	37.49897068898897	39.0	35.0	41.0	33.0	41.0
35	37.30798695556645	39.0	35.0	41.0	32.0	41.0
36	37.226084754183	39.0	35.0	41.0	32.0	41.0
37	37.189739035564806	39.0	35.0	41.0	32.0	41.0
38	37.19523194132819	39.0	35.0	41.0	32.0	41.0
39	37.212006139066304	39.0	35.0	41.0	32.0	41.0
40	37.13990836859075	39.0	35.0	41.0	32.0	41.0
41	37.01939347496913	39.0	35.0	41.0	32.0	41.0
42	37.01536714798592	39.0	35.0	41.0	32.0	41.0
43	36.98089334671113	39.0	35.0	41.0	32.0	41.0
44	36.955449016634574	39.0	35.0	41.0	32.0	41.0
45	36.9319274741617	39.0	35.0	41.0	32.0	41.0
46	36.83317152261183	38.0	35.0	40.0	32.0	41.0
47	36.748034189144285	38.0	35.0	40.0	32.0	41.0
48	36.71058177177869	38.0	35.0	40.0	32.0	41.0
49	36.68984997602604	38.0	35.0	40.0	32.0	41.0
50	36.59715895008112	38.0	35.0	40.0	32.0	41.0
51	35.90324747082807	37.0	34.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	7.0
10	21.0
11	16.0
12	27.0
13	32.0
14	45.0
15	65.0
16	87.0
17	144.0
18	243.0
19	404.0
20	734.0
21	1129.0
22	1838.0
23	2731.0
24	4285.0
25	6214.0
26	8646.0
27	11159.0
28	14233.0
29	18531.0
30	24219.0
31	32500.0
32	46365.0
33	72728.0
34	127035.0
35	159038.0
36	164726.0
37	254221.0
38	388972.0
39	506976.0
40	463.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.16744541458721	4.275428906646579	52.67063454696787	16.88649113179835
2	19.133224882267818	4.206916407174222	52.11852987112506	24.541328839432893
3	20.38392976007637	4.179208350515576	53.533751335344334	21.903110554063723
4	18.702559423499245	4.2595725382852825	49.706143070983494	27.331724967231974
5	15.438582819489588	19.627315814481573	43.484818474346774	21.449282891682063
6	20.54709341403305	9.353742048815967	51.57221574618555	18.52694879096544
7	81.10602769290381	2.306858068726804	8.215817620375812	8.371296617993568
8	82.5474419294332	2.403132742155968	9.71270208752066	5.336723240890165
9	78.42051088894156	4.468519426486521	11.918036104896641	5.192933579675275
10	44.54670809887014	23.122697985429458	15.97721228809019	16.353381627610215
11	30.89594434144119	26.419740258615743	22.57724973726052	20.10706566268255
12	27.118394577879663	19.557504499853344	28.749868765551955	24.574232156715038
13	25.32673318764957	21.066565359084507	29.786756198324742	23.81994525494118
14	21.23557368124262	22.771260251169203	31.320602780113845	24.672563287474336
15	19.150325948486827	20.54444166642314	36.47992951763088	23.82530286745916
16	24.835131651151237	20.267469334432995	31.264753728411254	23.632645286004507
17	23.844298039113816	20.882241841546715	26.729615907888025	28.543844211451436
18	23.724752927475244	23.279042859817796	30.148476219235672	22.84772799347129
19	24.456364681319467	22.91369697993006	29.92843528491134	22.701503053839136
20	25.05111378811346	26.12869742910363	28.80490605778212	20.015282725000784
21	24.91609112920072	26.69319496622539	28.255831950636367	20.13488195393752
22	25.475718109487953	22.38210276009044	28.27596358555241	23.866215544869192
23	23.02923741150469	24.366205262582543	28.104032929293588	24.500524396619184
24	22.468365733359743	27.09853352945442	27.145074405873242	23.288026331312594
25	22.899842951600736	25.640234695898666	25.57740451273326	25.88251783976734
26	20.887058281083082	25.673138013180807	27.128406278039524	26.31139742769658
27	21.206079753744646	22.791608355277898	29.54550128312114	26.45681060785632
28	21.36101757838079	22.793340108819063	28.650942344512885	27.19469996828726
29	19.909158703306243	25.10284992515578	28.078056626176107	26.909934745361873
30	22.934748608914852	24.854126822805895	27.82424649779905	24.386878070480204
31	25.595533807617333	24.30121038749068	25.52740012923211	24.57585567565988
32	23.966386663765977	27.303692206784362	23.49464617569289	25.23527495375677
33	23.691849610193103	24.085498837019262	24.815595306514965	27.40705624627267
34	22.88290423727621	25.020429280055932	27.475081690061575	24.621584792606278
35	22.735055778699216	27.150973191372834	25.447847700934823	24.666123328993127
36	23.91989990464532	26.446365969311163	25.168007152142124	24.46572697390139
37	23.16209537849097	27.383948159957743	24.483639799592822	24.97031666195846
38	23.746724550528782	26.599680275002463	23.188234033502937	26.465361140965822
39	22.781921358907002	23.89316595935358	26.735244106896815	26.5896685748426
40	24.00107585188745	21.635338162761023	29.47076529436022	24.89282069099131
41	22.002199326997278	25.927056376154184	25.857028592333307	26.213715704515224
42	21.592423145319017	24.909326466930544	25.67660152026314	27.821648867487305
43	21.370325753664552	24.78723784227838	27.256068984402308	26.586367419654756
44	22.681966709202865	22.454674056924905	27.57205988836684	27.291299345505397
45	21.831513368596163	22.748098047556116	25.896967158376437	29.52342142547128
46	22.571513303655408	22.72731700506213	28.224173331211933	26.476996360070526
47	20.88538064484008	23.507093154270017	30.470690612488756	25.136835588401148
48	20.19094747483275	24.920528747649957	27.84897810305882	27.039545674458477
49	22.724340553663254	22.92857923692445	28.49335277226683	25.853727437145462
50	21.453071102553363	22.145501932528717	28.4723552605802	27.929071704337716
51	21.3034908904352	23.531878876827946	25.339126048928534	29.82550418380832
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	487.0
1	1147.0
2	1807.0
3	2610.5
4	3414.0
5	2493.0
6	1572.0
7	1739.5
8	1907.0
9	2058.0
10	2209.0
11	2377.5
12	2546.0
13	2453.0
14	2360.0
15	2305.0
16	2250.0
17	2313.5
18	2377.0
19	2586.0
20	2795.0
21	3257.0
22	3719.0
23	4539.0
24	5359.0
25	5243.5
26	7488.0
27	9848.0
28	13186.0
29	16524.0
30	24849.0
31	33174.0
32	29338.5
33	25503.0
34	29205.0
35	32907.0
36	36177.0
37	39447.0
38	48887.5
39	58328.0
40	63823.5
41	69319.0
42	77785.5
43	86252.0
44	98079.5
45	109907.0
46	134769.0
47	159631.0
48	188192.5
49	216754.0
50	209925.5
51	203097.0
52	174178.0
53	145259.0
54	130244.0
55	115229.0
56	110008.5
57	104788.0
58	93684.0
59	82580.0
60	76827.5
61	71075.0
62	68994.0
63	66913.0
64	63447.0
65	59981.0
66	49498.5
67	39016.0
68	31365.0
69	23714.0
70	20455.5
71	17197.0
72	14082.5
73	10968.0
74	8360.0
75	4646.0
76	3540.0
77	2669.5
78	1799.0
79	1370.5
80	942.0
81	629.0
82	316.0
83	192.5
84	69.0
85	73.0
86	77.0
87	39.0
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1847838.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.41028990920343
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.7392707006818	15.623856754317671
2	12.695775626914052	5.944235760965137
3	5.767106527244439	4.050289072201552
4	3.3193001616390383	3.1082311631854274
5	1.9512920860076624	2.2840156715486843
6	1.3242399161174816	1.8600504207389685
7	0.8924015615805384	1.4623965490418385
8	0.644049654415497	1.2061911300631252
9	0.5012197979644375	1.056033070072187
>10	4.3859995968403265	23.92769272145316
>50	1.5693503493110637	25.398254122462916
>100	0.19614282670914526	7.2777953094154135
>500	0.007172940047524385	1.1313795589328366
>1k	0.0046995124449297696	2.187021134187688
>5k	0.0017313993218162308	2.6537246115917856
>10k+	2.473427602594615E-4	0.8288329498216195
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	14314	0.7746350058825503	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT	8847	0.47877573683407315	No Hit
CTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTTCTGCT	6726	0.3639929474337036	No Hit
GCTCAACTTGACCCCTGTCTCTTATACACATCTGACGCACGCTGAATCGTA	6707	0.3629647187686367	No Hit
CCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTTCTGC	6396	0.34613423904043533	No Hit
GCTCAACTTGACCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATG	6096	0.3298990495920097	No Hit
TCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTTCTGC	5917	0.32021205322111573	No Hit
GCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTTCTGC	5141	0.2782170298478546	No Hit
GCTCAACTTGACCCTGCATTAGAACTGTCTCTTATACACATCTGACGCACG	4793	0.25938421008768087	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	3909	0.2115445185129865	No Hit
GCTCCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTTC	2683	0.14519671096708694	No Hit
GCTCAACCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTC	2677	0.14487200717811843	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACCTGTCTCTTATACACATC	2582	0.13973086385278363	No Hit
GCCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTTCTG	2487	0.13458972052744883	No Hit
TCCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTTCTG	2311	0.12506507605103911	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGCTGTCTCTTATACACATCTG	1977	0.10698989846512519	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0823459632283782E-4	0.0	0.0	0.37838814874464105	0.0
2	1.0823459632283782E-4	0.0	0.0	1.3901110378723676	0.0
3	1.0823459632283782E-4	0.0	0.0	2.0122434975360393	0.0
4	1.0823459632283782E-4	0.0	0.0	2.689521484026197	0.0
5	1.0823459632283782E-4	0.0	0.0	3.871822096958716	0.0
6	1.0823459632283782E-4	0.0	0.0	4.6232948992281795	0.0
7	1.0823459632283782E-4	0.0	0.0	5.319026884391381	0.0
8	1.0823459632283782E-4	0.0	0.0	6.598792751312615	0.0
9	1.0823459632283782E-4	0.0	0.0	7.181257231423967	0.0
10	1.0823459632283782E-4	0.0	0.0	8.259544397290238	0.0
11	1.0823459632283782E-4	0.0	0.0	10.34809328523388	0.0
12	1.0823459632283782E-4	0.0	0.0	12.244363412809998	0.0
13	1.0823459632283782E-4	0.0	0.0	13.171609199507749	0.0
14	1.0823459632283782E-4	0.0	0.0	13.374873771402038	0.0
15	1.0823459632283782E-4	0.0	0.0	14.053667042240717	0.0
16	1.0823459632283782E-4	0.0	0.0	14.763631876820371	0.0
17	1.0823459632283782E-4	0.0	0.0	15.757496057554828	0.0
18	1.0823459632283782E-4	0.0	0.0	16.85910777892867	0.0
19	1.0823459632283782E-4	0.0	0.0	17.642239200622566	0.0
20	1.0823459632283782E-4	0.0	0.0	18.56883557974238	0.0
21	1.0823459632283782E-4	0.0	0.0	19.76066083715131	0.0
22	1.6235189448425674E-4	0.0	0.0	20.962714263912744	0.0
23	1.6235189448425674E-4	0.0	0.0	22.127967927924416	0.0
24	1.6235189448425674E-4	0.0	0.0	23.036489129458317	0.0
25	1.6235189448425674E-4	0.0	0.0	24.048644956971337	0.0
26	1.6235189448425674E-4	0.0	0.0	24.731172321383152	0.0
27	1.6235189448425674E-4	0.0	0.0	25.4072056100156	0.0
28	1.6235189448425674E-4	0.0	0.0	26.089787091725572	0.0
29	1.6235189448425674E-4	0.0	0.0	26.88303844817565	0.0
30	1.6235189448425674E-4	0.0	0.0	27.671906303474657	0.0
31	1.6235189448425674E-4	0.0	0.0	28.35124074729495	0.0
32	1.6235189448425674E-4	0.0	0.0	29.135725101442876	0.0
33	1.6235189448425674E-4	0.0	0.0	29.791247934072143	0.0
34	2.1646919264567565E-4	0.0	0.0	30.67703987037825	0.0
35	2.1646919264567565E-4	0.0	0.0	31.43424910625282	0.0
36	2.1646919264567565E-4	0.0	0.0	32.10465419587648	0.0
37	2.1646919264567565E-4	0.0	0.0	32.802388521071656	0.0
38	2.705864908070946E-4	0.0	0.0	33.6049480528055	0.0
39	2.705864908070946E-4	0.0	0.0	34.854895288439785	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACATA	20	7.0344E-4	45.000004	39
TAACGAC	20	7.0344E-4	45.000004	19
ACGGATA	20	7.0344E-4	45.000004	40
TTACGAA	20	7.0344E-4	45.000004	31
TAACACG	25	3.89173E-5	45.0	34
CAACGTA	30	2.1662217E-6	44.999996	27
CCGGTAA	30	2.1662217E-6	44.999996	32
ACTTGAC	5650	0.0	43.725666	6
CTCAACT	5860	0.0	43.61775	2
TCAACTT	5800	0.0	43.603447	3
TTGACCC	5265	0.0	43.16239	8
CTTGACC	5910	0.0	43.134518	7
TTACACG	230	0.0	43.04348	34
CGAACGA	110	0.0	42.954544	22
CAACTTG	5790	0.0	42.784973	4
AACTTGA	5755	0.0	42.732407	5
GCTCAAC	6210	0.0	42.644928	1
TGCATTA	4375	0.0	42.48	15
CATTAGA	4285	0.0	42.47958	17
GCATTAG	4410	0.0	42.34694	16
>>END_MODULE