##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935304.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3063219
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22037928075009	33.0	31.0	34.0	30.0	34.0
2	32.37418251845526	34.0	31.0	34.0	30.0	34.0
3	32.407271892737676	34.0	31.0	34.0	30.0	34.0
4	35.909507612743326	37.0	35.0	37.0	35.0	37.0
5	32.01992283281084	37.0	35.0	37.0	0.0	37.0
6	33.615069637528364	37.0	35.0	37.0	17.0	37.0
7	35.29186976184204	37.0	35.0	37.0	32.0	37.0
8	35.810901864998876	37.0	35.0	37.0	35.0	37.0
9	37.85121174816427	39.0	38.0	39.0	35.0	39.0
10	37.44152736059681	39.0	37.0	39.0	35.0	39.0
11	37.32032577494459	39.0	37.0	39.0	34.0	39.0
12	37.247294431119684	39.0	37.0	39.0	34.0	39.0
13	37.25310694403502	39.0	37.0	39.0	34.0	39.0
14	38.43656623963223	40.0	38.0	41.0	34.0	41.0
15	38.493024168366674	40.0	38.0	41.0	34.0	41.0
16	38.53920663197767	40.0	38.0	41.0	34.0	41.0
17	38.53851944637324	40.0	38.0	41.0	34.0	41.0
18	38.513291410114654	40.0	38.0	41.0	34.0	41.0
19	38.52946165455359	40.0	38.0	41.0	34.0	41.0
20	38.45608753406139	40.0	38.0	41.0	34.0	41.0
21	38.40826006890138	40.0	38.0	41.0	34.0	41.0
22	38.390058954322235	40.0	38.0	41.0	34.0	41.0
23	38.331387667679	40.0	38.0	41.0	34.0	41.0
24	38.311397258896605	40.0	37.0	41.0	34.0	41.0
25	38.214492662783826	40.0	37.0	41.0	34.0	41.0
26	38.171377560664126	40.0	37.0	41.0	34.0	41.0
27	38.15162252519327	40.0	37.0	41.0	34.0	41.0
28	38.137675105828215	40.0	37.0	41.0	34.0	41.0
29	38.082260523978206	40.0	37.0	41.0	34.0	41.0
30	37.99755845076699	40.0	37.0	41.0	34.0	41.0
31	37.82087764537893	40.0	37.0	41.0	33.0	41.0
32	37.817991792294315	40.0	37.0	41.0	33.0	41.0
33	37.760209113354286	40.0	37.0	41.0	33.0	41.0
34	37.76764149086304	40.0	37.0	41.0	33.0	41.0
35	37.67384636880354	40.0	37.0	41.0	33.0	41.0
36	37.63323647444078	40.0	37.0	41.0	33.0	41.0
37	37.6621067576298	40.0	37.0	41.0	33.0	41.0
38	37.66996385175203	40.0	37.0	41.0	33.0	41.0
39	37.5884685358768	39.0	36.0	41.0	33.0	41.0
40	37.54681790626135	39.0	36.0	41.0	33.0	41.0
41	37.51186382690888	39.0	36.0	41.0	33.0	41.0
42	37.46481332219472	39.0	36.0	41.0	33.0	41.0
43	37.42741736715527	39.0	36.0	41.0	33.0	41.0
44	37.43098061222524	39.0	36.0	41.0	33.0	41.0
45	37.44105596106579	39.0	36.0	41.0	33.0	41.0
46	37.31812449583265	39.0	36.0	41.0	32.0	41.0
47	37.250366036512574	39.0	35.0	41.0	32.0	41.0
48	37.222169880769215	39.0	35.0	41.0	32.0	41.0
49	37.199251506340225	39.0	35.0	41.0	32.0	41.0
50	37.14141169795565	39.0	35.0	41.0	32.0	41.0
51	36.406848808394045	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	6.0
10	10.0
11	15.0
12	23.0
13	20.0
14	38.0
15	59.0
16	88.0
17	154.0
18	233.0
19	426.0
20	746.0
21	1297.0
22	2309.0
23	3613.0
24	5833.0
25	9210.0
26	12795.0
27	16415.0
28	20864.0
29	27518.0
30	37188.0
31	50521.0
32	69367.0
33	101385.0
34	160522.0
35	216276.0
36	267626.0
37	433095.0
38	730426.0
39	894481.0
40	657.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.18842955727292	4.442940579827952	59.436462100816165	14.93216776208296
2	19.609665518528058	4.418652404545675	55.18325656768256	20.78842550924371
3	19.152825834522442	4.160296733599524	55.051467100458694	21.635410331419333
4	18.65668109266755	4.150601050724744	52.72747394162807	24.465243914979634
5	15.56447645434427	15.57635937881033	46.74807775741793	22.111086409427468
6	19.725719904453452	5.896868620885415	56.09164738139846	18.285764093262678
7	80.9937846428871	1.7227955298005138	11.300889685001302	5.982530142311079
8	82.76528710483971	1.8128641798056226	8.759967863871307	6.661880851483358
9	77.83282879872448	4.108031453186991	11.692895610793745	6.366244137294787
10	35.89713957767956	23.50429401227924	22.654664912955948	17.943901497085253
11	24.40791859804996	26.264984645237575	26.25669271442884	23.070404042283624
12	19.88966508760882	22.525748240657947	34.338452458018836	23.2461342137144
13	20.603064945731926	24.840013071216912	35.27573444797776	19.2811875350734
14	19.575159334020846	26.345912584114945	32.967313143461176	21.111614938403033
15	17.110007479060428	26.955565370938217	34.309234827806954	21.625192322194398
16	18.05489584649351	25.774814011012598	32.13358235242077	24.036707790073123
17	18.092209535132813	27.112002112810085	29.67998696795756	25.11580138409954
18	19.279130875069658	24.953553761582178	33.057283857275635	22.710031506072532
19	19.859631322474822	27.324784809705083	30.688174759950236	22.12740910786986
20	20.980576315307527	28.17232460362775	30.68928470344432	20.157814377620404
21	21.54661485189273	28.306236021649124	30.152235279292793	19.994913847165353
22	19.59615032421776	26.237562511854364	29.213908636633555	24.95237852729433
23	18.535403443240593	26.983607766862246	28.07970961266563	26.401279177231533
24	20.05432846949565	25.977901025032818	29.471480818054474	24.49628968741706
25	17.92206172656934	28.73953837450081	27.085265532761447	26.2531343661684
26	17.611408129813768	29.390095843620713	29.203657982011734	23.794838044553785
27	18.801822527217283	27.158946193530404	31.014628728798037	23.024602550454276
28	16.916812020296295	25.802595243761544	31.011266252918908	26.269326483023253
29	18.777632288125663	24.91196352595097	31.239784031112368	25.070620154811003
30	20.913261506931107	25.974048868200413	31.22006621139396	21.892623413474517
31	21.84659340386698	27.80767552042476	26.78956352777911	23.556167547929157
32	21.381331207465088	28.309010880384328	27.173734558319207	23.135923353831377
33	20.90506751231303	24.8867939249528	30.48570800847083	23.72243055426334
34	20.647952366448497	24.57140674564894	28.8568985763016	25.923742311600968
35	20.755029268230576	25.860508177835147	28.673039701046516	24.71142285288776
36	20.148934829667745	25.90281661219782	32.117227008581494	21.831021549552936
37	19.84575702879879	25.355255370249402	31.51821662114266	23.280770979809148
38	21.91106806271442	25.191310187094036	28.80358864318875	24.094033107002797
39	22.706571094002747	22.908711391513307	32.31802884481978	22.066688669664167
40	24.468965490224498	23.117315477607054	30.589357143580003	21.82436188858844
41	22.81224424371878	24.981465575918666	28.03567097226806	24.17061920809449
42	21.77908272310925	23.876255664384427	30.620239689033006	23.72442192347331
43	20.828971092174605	23.272479049000413	31.816922002638403	24.08162785618658
44	19.781608823920198	23.03890123428981	29.112087643749923	28.06740229804007
45	22.05490368138876	22.640660037692374	27.757009864459576	27.547426416459288
46	23.351807363430428	22.641900562773998	30.78715560330489	23.219136470490685
47	19.60982874551248	24.258598552698974	32.52532711503813	23.606245586750408
48	19.851861718016245	22.613629649071775	32.634754485395916	24.89975414751606
49	22.506128357130194	20.183702177350035	33.89698875594595	23.413180709573815
50	22.83039508438672	19.095794326164732	31.12944258964181	26.94436799980674
51	21.511553695638476	19.249750017873353	28.54947687383762	30.689219412650548
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	644.0
1	1181.5
2	1719.0
3	4010.0
4	6301.0
5	4486.5
6	2672.0
7	3052.5
8	3433.0
9	4327.0
10	5221.0
11	5600.5
12	5980.0
13	6590.5
14	7201.0
15	7472.0
16	7743.0
17	7819.5
18	7896.0
19	8015.0
20	8134.0
21	8818.0
22	9502.0
23	10361.5
24	11221.0
25	12855.0
26	20298.5
27	26108.0
28	28854.0
29	31600.0
30	37534.0
31	43468.0
32	53399.5
33	63331.0
34	69134.0
35	74937.0
36	86216.5
37	97496.0
38	108140.0
39	118784.0
40	135648.0
41	152512.0
42	179228.0
43	205944.0
44	234783.5
45	263623.0
46	322724.5
47	381826.0
48	427003.5
49	472181.0
50	433189.0
51	394197.0
52	313095.0
53	231993.0
54	186890.0
55	141787.0
56	115458.0
57	89129.0
58	75434.0
59	61739.0
60	52343.5
61	42948.0
62	36297.0
63	29646.0
64	24604.5
65	19563.0
66	15386.0
67	11209.0
68	8999.0
69	6789.0
70	5309.5
71	3830.0
72	3303.5
73	2777.0
74	2357.0
75	1324.5
76	712.0
77	602.5
78	493.0
79	398.5
80	304.0
81	209.5
82	115.0
83	80.0
84	45.0
85	38.5
86	32.0
87	19.0
88	6.0
89	4.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3063219.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.027127430638743
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.771645072523526	16.695101229752815
2	14.804861792288582	8.002657725063564
3	7.101968607506513	5.758374316904236
4	3.962382548024443	4.283672722175826
5	2.434129611287145	3.2893765593474407
6	1.596729115588374	2.5893000767530854
7	1.1386659480758814	2.154240877570315
8	0.8369986458555979	1.8097335248649051
9	0.6504516120954216	1.5821854746810624
>10	5.0374484325671265	29.300790868179345
>50	0.5140336076550476	9.024951897282932
>100	0.13367067780804193	6.861512966387857
>500	0.009522945181638303	1.8111851096561413
>1k	0.006602575325935676	3.5011624091014006
>5k	3.8091780726551977E-4	0.7053182681943571
>10k+	5.078904096873597E-4	2.6304359740848375
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCTGCT	22919	0.7481998512022809	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCTGC	22385	0.7307672092658083	No Hit
TCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCTGC	18514	0.6043968779248236	No Hit
GCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCTGC	12833	0.41893837822238633	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCC	7508	0.24510163981093092	No Hit
TCCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCTG	7478	0.24412227790438754	No Hit
TTCCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCT	5567	0.18173692445757225	No Hit
CGTTCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTC	4558	0.1487977190008289	No Hit
TGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCTGCTT	4049	0.13218121198647567	Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCTG	4032	0.13162624023943442	No Hit
TGCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCTG	3781	0.12343224562135453	No Hit
TTCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCTG	3709	0.12108177704565033	No Hit
TTTCCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTC	3655	0.11931892561387221	No Hit
GCCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCTG	3562	0.11628290370358763	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTC	3434	0.11210429290233574	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3104	0.10133131193035823	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.793619065434108E-5	0.0	0.0	0.7702354940995078	0.0
2	9.793619065434108E-5	0.0	0.0	2.6298152368472514	0.0
3	9.793619065434108E-5	0.0	0.0	3.678418030183281	0.0
4	1.3058158753912142E-4	0.0	0.0	4.955897701078506	0.0
5	1.3058158753912142E-4	0.0	0.0	7.063386587769272	0.0
6	1.3058158753912142E-4	0.0	0.0	8.567457958441757	0.0
7	1.3058158753912142E-4	0.0	0.0	9.85182580807967	0.0
8	1.3058158753912142E-4	0.0	0.0	11.796610036696691	0.0
9	1.3058158753912142E-4	0.0	0.0	12.602135204828645	0.0
10	1.3058158753912142E-4	0.0	0.0	14.294864324098278	0.0
11	1.3058158753912142E-4	0.0	0.0	17.830099643544912	0.0
12	1.3058158753912142E-4	0.0	0.0	20.62781015657059	0.0
13	1.3058158753912142E-4	0.0	0.0	21.519584463272132	0.0
14	1.3058158753912142E-4	0.0	0.0	21.805656076173463	0.0
15	1.3058158753912142E-4	0.0	0.0	22.207977947381497	0.0
16	1.3058158753912142E-4	0.0	0.0	23.313122568121965	0.0
17	1.3058158753912142E-4	0.0	0.0	25.084461803090147	0.0
18	1.3058158753912142E-4	0.0	0.0	26.955108335381833	0.0
19	1.3058158753912142E-4	0.0	0.0	28.297584991474654	0.0
20	1.6322698442390178E-4	0.0	0.0	29.56974346267766	0.0
21	2.285177781934625E-4	0.0	0.0	31.455765976902075	0.0
22	2.285177781934625E-4	0.0	0.0	33.388047018512225	0.0
23	2.285177781934625E-4	0.0	0.0	35.27371043337091	0.0
24	2.285177781934625E-4	0.0	0.0	36.68947600547006	0.0
25	2.6116317507824283E-4	0.0	0.0	37.93473466964001	0.0
26	2.6116317507824283E-4	0.0	0.0	38.96322789849501	0.0
27	2.6116317507824283E-4	0.0	0.0	40.010883975321384	0.0
28	2.6116317507824283E-4	0.0	0.0	41.106920530331	0.0
29	2.938085719630232E-4	0.0	0.0	42.22283813204345	0.0
30	2.938085719630232E-4	0.0	0.0	43.38883377257715	0.0
31	2.938085719630232E-4	0.0	0.0	44.49143205236061	0.0
32	2.938085719630232E-4	0.0	0.0	45.52935980091531	0.0
33	2.938085719630232E-4	0.0	0.0	46.515446659216984	0.0
34	2.938085719630232E-4	0.0	0.0	47.51031512928067	0.0
35	2.938085719630232E-4	0.0	0.0	48.51863350286088	0.0
36	2.938085719630232E-4	0.0	0.0	49.454022059800494	0.0
37	2.938085719630232E-4	0.0	0.0	50.39123875896565	0.0
38	2.938085719630232E-4	0.0	0.0	51.33446221115761	0.0
39	2.938085719630232E-4	0.0	0.0	52.26155883728848	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGAAT	20	7.0351287E-4	45.0	27
TCGCGAT	25	3.892333E-5	45.0	11
CCCGATA	30	2.166691E-6	44.999996	30
TACGGGA	915	0.0	43.770493	4
CGCACGG	100	0.0	42.75	2
TTTACGG	1160	0.0	42.478447	2
TGTTACG	245	0.0	42.2449	1
TTACGGG	1905	0.0	42.047245	3
TTTTACG	830	0.0	41.746986	1
TACGGGT	505	0.0	41.435642	4
CGTTTTT	3680	0.0	41.392666	1
TTATGGG	6700	0.0	40.97015	3
GTTACGG	385	0.0	40.909092	2
TTTATGG	4085	0.0	40.758873	2
TTGTACG	205	0.0	40.609756	1
CTACGGG	795	0.0	40.18868	3
TACGGGG	1370	0.0	39.744526	4
TTCTACG	340	0.0	39.705883	1
GCGATGC	465	0.0	39.67742	9
TATGGGA	3485	0.0	39.57676	4
>>END_MODULE