##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935303.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1253326
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21568211303364	33.0	31.0	34.0	30.0	34.0
2	32.37118754418244	34.0	31.0	34.0	30.0	34.0
3	32.43680096000562	34.0	31.0	34.0	30.0	34.0
4	35.89811030809223	37.0	35.0	37.0	35.0	37.0
5	31.567195605931737	37.0	35.0	37.0	0.0	37.0
6	33.361166208951225	37.0	35.0	37.0	17.0	37.0
7	35.16989274937247	37.0	35.0	37.0	32.0	37.0
8	35.72744282014416	37.0	35.0	37.0	35.0	37.0
9	37.772059304602315	39.0	38.0	39.0	35.0	39.0
10	37.4795639761722	39.0	37.0	39.0	35.0	39.0
11	37.35816539352092	39.0	37.0	39.0	35.0	39.0
12	37.09427315798124	39.0	37.0	39.0	34.0	39.0
13	37.00458539916989	39.0	37.0	39.0	33.0	39.0
14	38.126508187015986	40.0	37.0	41.0	33.0	41.0
15	38.20978261043017	40.0	37.0	41.0	34.0	41.0
16	38.27916998450523	40.0	37.0	41.0	34.0	41.0
17	38.26825103763905	40.0	37.0	41.0	34.0	41.0
18	38.262707388181525	40.0	37.0	41.0	34.0	41.0
19	38.29236447660066	40.0	37.0	41.0	34.0	41.0
20	38.22371513875879	40.0	37.0	41.0	34.0	41.0
21	38.16343872224784	40.0	37.0	41.0	34.0	41.0
22	38.13058613640825	40.0	37.0	41.0	34.0	41.0
23	38.11005197370836	40.0	37.0	41.0	34.0	41.0
24	38.078824663335794	40.0	36.0	41.0	34.0	41.0
25	37.9831408588029	40.0	36.0	41.0	34.0	41.0
26	37.95267711672781	40.0	36.0	41.0	34.0	41.0
27	37.88941664020375	40.0	36.0	41.0	34.0	41.0
28	37.823625297807595	40.0	36.0	41.0	33.0	41.0
29	37.77600959367315	40.0	36.0	41.0	33.0	41.0
30	37.694364435111055	40.0	36.0	41.0	33.0	41.0
31	37.38336075370654	39.0	35.0	41.0	33.0	41.0
32	37.44951911952676	39.0	35.0	41.0	33.0	41.0
33	37.485348584486395	39.0	35.0	41.0	33.0	41.0
34	37.39281639413848	39.0	35.0	41.0	33.0	41.0
35	37.348794328051916	39.0	35.0	41.0	33.0	41.0
36	37.38078600459896	39.0	35.0	41.0	33.0	41.0
37	37.29243867916249	39.0	35.0	41.0	33.0	41.0
38	37.28163143507754	39.0	35.0	41.0	33.0	41.0
39	37.3032259763222	39.0	35.0	41.0	33.0	41.0
40	37.20909244681751	39.0	35.0	41.0	33.0	41.0
41	37.15522138693365	39.0	35.0	41.0	32.0	41.0
42	37.190006430888694	39.0	35.0	41.0	33.0	41.0
43	37.177498113020874	39.0	35.0	41.0	33.0	41.0
44	37.111775388047484	39.0	35.0	41.0	32.0	41.0
45	37.0604519494529	39.0	35.0	41.0	32.0	41.0
46	36.91756893258418	39.0	35.0	41.0	32.0	41.0
47	36.85233769984824	39.0	35.0	40.0	32.0	41.0
48	36.824409610907296	39.0	35.0	40.0	32.0	41.0
49	36.82018644789943	39.0	35.0	40.0	32.0	41.0
50	36.73147529054691	39.0	35.0	40.0	32.0	41.0
51	36.07570496423117	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	12.0
11	16.0
12	15.0
13	21.0
14	17.0
15	21.0
16	41.0
17	67.0
18	137.0
19	239.0
20	444.0
21	672.0
22	1115.0
23	1763.0
24	2613.0
25	4020.0
26	5640.0
27	7455.0
28	9643.0
29	12661.0
30	16831.0
31	22803.0
32	31564.0
33	48741.0
34	82167.0
35	115840.0
36	111200.0
37	173247.0
38	271164.0
39	332850.0
40	299.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.851575727304787	4.385770342273279	55.53670792754638	16.22594600287555
2	19.536018561810735	4.477605985992471	53.577840083106864	22.408535369089925
3	21.14916629831345	4.518058350341411	51.12197464985167	23.21080070149347
4	19.286043694936513	4.724947858737472	50.70691902984539	25.28208941648063
5	15.7826455367558	16.944753400152873	44.37847774641235	22.89412331667898
6	21.668664018778834	6.249451459556412	52.874671075203096	19.207213446461655
7	81.3943858182149	2.1363156912088317	10.754185263849948	5.715113226726327
8	82.45221115655464	2.6767975770070995	8.205766097567592	6.665225168870668
9	77.29976079647275	4.991279204293217	10.903946778412003	6.805013220822037
10	39.28140005074498	25.7021716616427	18.106542112746403	16.909886174865917
11	29.64719474422457	24.656154903034007	24.859533752591105	20.83711660015032
12	25.491133192800596	22.099597391261334	31.07874567351192	21.330523742426152
13	22.50037101280912	24.916422383322455	31.82500003989385	20.758206563974575
14	19.411310385326722	26.969359927105955	31.94595819443624	21.67337149313108
15	17.755635804252044	24.739453262758452	36.042498120999646	21.462412811989857
16	19.702455705857854	24.5440531832899	31.841356518575374	23.91213459227687
17	18.755934210253358	24.38878631736675	29.449560609131225	27.40571886324867
18	20.2161289241586	23.439791403034803	32.70649455927668	23.63758511352992
19	21.603716830258048	24.700357289324565	30.350443539829225	23.345482340588163
20	24.262083448360602	26.761991692504584	29.069771152916317	19.906153706218493
21	23.013086778699236	26.835077226515686	29.91679738551662	20.235038609268457
22	21.85967577469868	24.01825223445456	29.066340281778245	25.055731709068514
23	21.238129584800763	24.821873957773157	27.850056569479925	26.08993988794615
24	22.178268064334418	23.356732406413016	29.938659215559237	24.526340313693325
25	20.28642188863871	25.61225092274476	26.75520973792932	27.346117450687213
26	19.94724437217452	26.59691093937252	27.69734290998511	25.758501778467853
27	19.956978471682547	26.16414244976965	28.43051209342182	25.448366985125976
28	18.049972632818594	26.382601174794107	29.904350504178485	25.663075688208814
29	20.30317730582466	25.219934797490833	29.163521701456762	25.313366195227736
30	22.78529289267118	26.069913175023895	27.363590957181135	23.78120297512379
31	22.239305655511814	30.66672198613928	24.015220301820914	23.07875205652799
32	22.44340259437688	30.50810403677894	23.616680735897923	23.431812632946254
33	22.431992953150257	27.44880422172683	24.594558797950413	25.5246440271725
34	20.028548039376826	27.241914713330768	27.02369535140897	25.705841895883434
35	20.06876104062311	27.66135865688576	25.549617577549654	26.720262724941474
36	23.376759119335272	26.915104290503827	25.11948208207601	24.588654508084886
37	19.44282652717649	30.12376668161356	26.7805822268109	23.652824564399047
38	19.42758707630736	32.28250271677122	24.25609937079419	24.033810836127234
39	22.06201738414427	27.295133109821386	26.362015947965656	24.28083355806869
40	23.574393254428617	26.313185875023738	26.263717500474737	23.84870337007291
41	19.24614984449377	27.611411556131443	24.739373475057565	28.403065124317216
42	20.43977384974061	25.456505330616295	25.311132139602943	28.792588680040147
43	21.184352674403947	24.06085886672741	26.54145848725711	28.213329971611532
44	20.645147391819847	24.228492826287816	26.34222859814605	28.784131183746286
45	22.038559800083938	25.014481467710713	25.077673326812018	27.869285405393327
46	23.02912410657722	25.366744167120125	27.533299396964555	24.070832329338096
47	18.78545565958099	26.841061304082096	28.83415807220149	25.539324964135428
48	18.698726428718466	25.778927429894537	29.209240054064146	26.31310608732285
49	21.858798109988943	23.181757978371152	29.714854714575456	25.24458919706445
50	21.28672029464002	23.81351699398241	27.714178114871952	27.18558459650562
51	19.600646599527977	23.314684288046365	25.534138763577875	31.55053034884779
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	521.0
1	821.0
2	1121.0
3	1682.0
4	2243.0
5	1634.5
6	1026.0
7	1106.0
8	1186.0
9	1610.5
10	2035.0
11	2080.5
12	2126.0
13	2130.5
14	2135.0
15	2185.5
16	2236.0
17	2407.0
18	2578.0
19	2533.5
20	2489.0
21	2894.5
22	3300.0
23	3485.5
24	3671.0
25	4544.0
26	7360.5
27	9304.0
28	10139.5
29	10975.0
30	13259.0
31	15543.0
32	17988.5
33	20434.0
34	23983.5
35	27533.0
36	29951.0
37	32369.0
38	36630.5
39	40892.0
40	46782.0
41	52672.0
42	63591.0
43	74510.0
44	85704.5
45	96899.0
46	114733.0
47	132567.0
48	150818.0
49	169069.0
50	160754.5
51	152440.0
52	129095.5
53	105751.0
54	89753.5
55	73756.0
56	63810.5
57	53865.0
58	48446.0
59	43027.0
60	38882.0
61	34737.0
62	30524.5
63	26312.0
64	22005.5
65	17699.0
66	14469.5
67	11240.0
68	9090.0
69	6940.0
70	5660.0
71	4380.0
72	3846.0
73	3312.0
74	2893.5
75	1890.0
76	1305.0
77	1116.0
78	927.0
79	604.0
80	281.0
81	151.0
82	21.0
83	13.5
84	6.0
85	3.0
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1253326.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.38911682873252
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.50635783051648	17.286549298142567
2	12.872717132421046	6.7939927262137125
3	5.96029054800435	4.718604108134308
4	3.382279393295335	3.570214642283405
5	2.270947861436699	2.996415421370666
6	1.5875399660095897	2.5136266579985467
7	1.179203226116319	2.178269218916128
8	0.8862605420055347	1.8710106386943888
9	0.6858583724528273	1.6289277046759798
>10	4.412065080612153	24.881187389144667
>50	1.0807248385634474	19.573258375980856
>100	0.1637171805892336	7.1106319569942915
>500	0.008125668884498899	1.508946401699305
>1k	0.0027085562948329666	1.078420167824993
>5k	0.0012038027977035407	2.289945291926198
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGCT	7873	0.6281685690714147	Illumina Single End Adapter 1 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC	7634	0.6090993085597841	Illumina Single End Adapter 1 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC	7478	0.5966524272216487	Illumina Single End Adapter 1 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC	5849	0.46667826247919536	Illumina Single End Adapter 1 (95% over 24bp)
TCCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTG	2447	0.19524050406677912	Illumina Single End Adapter 1 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTG	1646	0.1313305556575065	Illumina Single End Adapter 1 (95% over 23bp)
TTCCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCT	1612	0.12861777382740006	Illumina Single End Adapter 1 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTG	1494	0.11920282512291294	Illumina Single End Adapter 1 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	1374	0.10962830101665488	No Hit
TGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGCTT	1336	0.10659636838300648	Illumina Single End Adapter 1 (96% over 26bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCC	1313	0.10476125126264037	No Hit
CGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTG	1278	0.10196868173164844	Illumina Single End Adapter 1 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.97877008854839E-5	0.0	0.0	0.6623177050504019	0.0
2	7.97877008854839E-5	0.0	0.0	2.49216883715809	0.0
3	7.97877008854839E-5	0.0	0.0	3.4391690589678983	0.0
4	7.97877008854839E-5	0.0	0.0	4.509840217150207	0.0
5	7.97877008854839E-5	0.0	0.0	6.212190603242892	0.0
6	7.97877008854839E-5	0.0	0.0	7.355548356931876	0.0
7	7.97877008854839E-5	0.0	0.0	8.37260218011914	0.0
8	7.97877008854839E-5	0.0	0.0	9.897265356339851	0.0
9	7.97877008854839E-5	0.0	0.0	10.569396948599167	0.0
10	7.97877008854839E-5	0.0	0.0	11.962171055256174	0.0
11	7.97877008854839E-5	0.0	0.0	14.692984905762746	0.0
12	7.97877008854839E-5	0.0	0.0	16.98105680405577	0.0
13	7.97877008854839E-5	0.0	0.0	17.72268348378634	0.0
14	7.97877008854839E-5	0.0	0.0	17.980397757646454	0.0
15	7.97877008854839E-5	0.0	0.0	18.350133963549787	0.0
16	7.97877008854839E-5	0.0	0.0	19.34955470484136	0.0
17	7.97877008854839E-5	0.0	0.0	20.752860788015248	0.0
18	7.180893079693552E-4	0.0	0.0	22.392657616613715	0.0
19	7.180893079693552E-4	0.0	0.0	23.494047039636936	0.0
20	7.180893079693552E-4	0.0	0.0	24.589452385093743	0.0
21	7.180893079693552E-4	0.0	0.0	26.04246620591929	0.0
22	7.180893079693552E-4	0.0	0.0	27.460373438355223	0.0
23	7.978770088548391E-4	0.0	0.0	28.801125964034895	0.0
24	7.978770088548391E-4	0.0	0.0	29.872116273020747	0.0
25	8.776647097403229E-4	0.0	0.0	30.817201590009304	0.0
26	9.574524106258068E-4	0.0	0.0	31.63965321073687	0.0
27	9.574524106258068E-4	0.0	0.0	32.426359941467744	0.0
28	9.574524106258068E-4	0.0	0.0	33.25519457826615	0.0
29	9.574524106258068E-4	0.0	0.0	34.11155597187005	0.0
30	9.574524106258068E-4	0.0	0.0	35.08017866061982	0.0
31	9.574524106258068E-4	0.0	0.0	35.95066247728045	0.0
32	0.0011170278123967747	0.0	0.0	36.76074700437077	0.0
33	0.0011968155132822587	0.0	0.0	37.539235601910434	0.0
34	0.0011968155132822587	0.0	0.0	38.34166050971575	0.0
35	0.0011968155132822587	0.0	0.0	39.215575197514454	0.0
36	0.0011968155132822587	0.0	0.0	39.979462645792076	0.0
37	0.0011968155132822587	0.0	0.0	40.78204712899916	0.0
38	0.0011968155132822587	0.0	0.0	41.603461509615215	0.0
39	0.0011968155132822587	0.0	0.0	42.480168766944914	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGAAC	30	2.1656597E-6	45.000004	11
ATTCGTT	30	2.1656597E-6	45.000004	11
ATTCGAT	30	2.1656597E-6	45.000004	14
CACGATA	20	7.03353E-4	45.000004	20
TGACGTA	20	7.03353E-4	45.000004	25
ACGATAC	20	7.03353E-4	45.000004	21
CGCTATC	20	7.03353E-4	45.000004	1
CTACGAA	80	0.0	45.000004	11
TACGAAT	80	0.0	45.000004	12
TACGAAG	20	7.03353E-4	45.000004	37
CGAGGAT	20	7.03353E-4	45.000004	37
GGTATCG	20	7.03353E-4	45.000004	20
AGCGAAT	20	7.03353E-4	45.000004	15
CCCGGTT	20	7.03353E-4	45.000004	3
CAATCGG	20	7.03353E-4	45.000004	20
GTGGTCG	30	2.1656597E-6	45.000004	18
CGTCATT	20	7.03353E-4	45.000004	21
AATCGAC	20	7.03353E-4	45.000004	19
CGGACAA	25	3.891009E-5	45.0	11
GGTCGAC	35	1.2120472E-7	45.0	20
>>END_MODULE