##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935302.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	491497
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33861651241005	33.0	31.0	34.0	30.0	34.0
2	32.48967948939668	34.0	31.0	34.0	31.0	34.0
3	32.53864418297569	34.0	31.0	34.0	30.0	34.0
4	35.97838847439557	37.0	35.0	37.0	35.0	37.0
5	31.667654126067912	37.0	35.0	37.0	0.0	37.0
6	33.48342105852121	37.0	35.0	37.0	17.0	37.0
7	35.2614705684877	37.0	35.0	37.0	32.0	37.0
8	35.802326362114115	37.0	35.0	37.0	35.0	37.0
9	37.8650917503057	39.0	38.0	39.0	35.0	39.0
10	37.57243075746139	39.0	37.0	39.0	35.0	39.0
11	37.47787270319046	39.0	37.0	39.0	35.0	39.0
12	37.05803494222752	39.0	35.0	39.0	34.0	39.0
13	36.801750570196766	39.0	35.0	39.0	33.0	39.0
14	37.88055878265788	40.0	36.0	41.0	33.0	41.0
15	38.03952211305461	40.0	36.0	41.0	33.0	41.0
16	38.15968561354393	40.0	36.0	41.0	34.0	41.0
17	38.21142753668893	40.0	36.0	41.0	34.0	41.0
18	38.23288646726226	40.0	36.0	41.0	34.0	41.0
19	38.25051831445563	40.0	36.0	41.0	34.0	41.0
20	38.145270469606125	40.0	36.0	41.0	34.0	41.0
21	38.0722242455193	40.0	36.0	41.0	34.0	41.0
22	38.0307306046629	40.0	36.0	41.0	34.0	41.0
23	37.99048620846109	40.0	35.0	41.0	34.0	41.0
24	37.99738553846717	40.0	35.0	41.0	34.0	41.0
25	37.891665666321465	40.0	35.0	41.0	34.0	41.0
26	37.8055735843759	40.0	35.0	41.0	34.0	41.0
27	37.73279592754381	39.0	35.0	41.0	34.0	41.0
28	37.68648638750592	39.0	35.0	41.0	34.0	41.0
29	37.679662337715186	39.0	36.0	41.0	34.0	41.0
30	37.70238068594519	39.0	36.0	41.0	34.0	41.0
31	37.44570160143399	39.0	35.0	41.0	33.0	41.0
32	37.482599079953694	39.0	35.0	41.0	33.0	41.0
33	37.501897264886665	39.0	35.0	41.0	33.0	41.0
34	37.49173443581548	39.0	35.0	41.0	33.0	41.0
35	37.432438041330876	39.0	35.0	41.0	33.0	41.0
36	37.39444187858725	39.0	35.0	41.0	33.0	41.0
37	37.347385640197196	39.0	35.0	41.0	33.0	41.0
38	37.33900308648883	39.0	35.0	41.0	33.0	41.0
39	37.39596579429783	39.0	35.0	41.0	33.0	41.0
40	37.33028685831246	39.0	35.0	41.0	33.0	41.0
41	37.300506412043205	39.0	35.0	41.0	33.0	41.0
42	37.28605464529794	39.0	35.0	41.0	33.0	41.0
43	37.21915087986295	39.0	35.0	41.0	33.0	41.0
44	37.1601678138422	39.0	35.0	41.0	33.0	41.0
45	37.1578768537753	39.0	35.0	41.0	33.0	41.0
46	37.08044606579491	39.0	35.0	41.0	33.0	41.0
47	37.04245600685253	39.0	35.0	41.0	33.0	41.0
48	37.04146108724977	39.0	35.0	41.0	33.0	41.0
49	37.073542666588	39.0	35.0	41.0	33.0	41.0
50	36.99771717833476	39.0	35.0	41.0	33.0	41.0
51	36.412488784265214	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	4.0
11	1.0
12	3.0
13	3.0
14	2.0
15	12.0
16	22.0
17	22.0
18	45.0
19	89.0
20	161.0
21	263.0
22	422.0
23	671.0
24	1015.0
25	1481.0
26	2094.0
27	2771.0
28	3416.0
29	4332.0
30	5898.0
31	8231.0
32	11791.0
33	19242.0
34	34731.0
35	57228.0
36	37151.0
37	58972.0
38	98092.0
39	143193.0
40	135.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.768806320282728	4.568288311017158	56.42862520015382	16.2342801685463
2	18.642433219327888	4.530037823221708	54.62373117231641	22.20379778513399
3	20.22901462267318	4.637057805032381	51.86623723033915	23.26769034195529
4	19.286587710606575	4.783142114804363	51.34985564510058	24.580414529488483
5	15.679037715387887	16.704679784413738	45.952264205071444	21.664018295126926
6	20.46808017139474	5.6010514814942916	55.205830350948226	18.725037996162744
7	81.37994738523328	2.428092134845177	10.633635607134936	5.558324872786609
8	82.53051391971874	2.7098842922744186	8.040333918620053	6.719267869386791
9	77.69894831504567	4.54509386629013	11.240149990742568	6.515807827921635
10	38.97399170289951	26.73749788910207	17.902042128436186	16.386468279562237
11	34.069180483298986	25.581234473455584	23.1181472114784	17.23143783176703
12	32.33936321076222	20.249360626819694	28.889291287637565	18.521984874780518
13	23.501059009515828	29.854098804265337	28.72896477496302	17.915877411255817
14	17.528286032264692	33.53489441441148	28.697021548452987	20.23979800487083
15	14.189710211862941	26.791007880007406	40.32313523785496	18.69614667027469
16	16.013526023556604	22.295151343751844	39.63605067782713	22.055271954864423
17	16.63570683035705	20.79768543856829	29.90577765479749	32.66083007627717
18	19.145386441829757	23.167181081471504	33.57029646162642	24.11713601507232
19	23.308382350248323	24.382447909142886	29.59224573089968	22.716924009709114
20	27.099656762910048	25.530776383172228	27.917769589641445	19.451797264276284
21	23.043273916219224	26.930784928493967	29.456537883242422	20.569403272044386
22	19.67865521050993	26.07136971334515	27.82336413040161	26.426610945743313
23	19.67845175046847	27.738724753152106	26.777172597187775	25.80565089919165
24	22.08945324183057	23.436969096454302	29.704148753705518	24.769428908009612
25	18.452808460682366	26.22335436431962	29.35663900288303	25.967198172114987
26	18.277629364980864	31.855942152241013	26.547262750332152	23.319165732445978
27	20.402769498084425	30.399982095516354	28.448596837824034	20.74865156857519
28	16.307932703556684	26.654893112267214	35.39411227332008	21.64306191085602
29	17.137642752651594	25.349900406309704	33.12695703127384	24.38549980976486
30	20.287204194532215	26.86140505435435	29.963967226656518	22.887423524456914
31	21.311218583226346	28.458973299938762	25.079298551161045	25.15050956567385
32	21.459134033371516	28.006681627761715	25.777980333552392	24.756204005314373
33	21.85913647489201	26.247769569295436	28.247985236939392	23.645108718873157
34	18.162470981511586	25.42904636243965	29.87892092932409	26.529561726724683
35	18.011300170702974	24.856509805756698	27.961716958597915	29.17047306494241
36	23.421506133302948	24.61256121603998	30.033957480920538	21.93197516973654
37	19.66441300760737	25.70697277908105	31.911079823478065	22.717534389833506
38	17.83001727375752	26.43047668653115	28.067923100242727	27.671582939468603
39	19.50225535455964	23.593022948258078	31.506601261045336	25.398120436136946
40	21.859543394974946	22.294337503585986	29.286241828536085	26.559877272902987
41	16.689420281303853	24.216424515307317	27.460391416427772	31.63376378696106
42	21.30531824202386	23.77186432470595	27.834350972640724	27.088466460629462
43	23.09678390712456	22.83350661346865	29.265692364348105	24.804017115058688
44	19.292081131726135	25.05467988614376	29.69885879262742	25.954380189502686
45	19.500424214186456	25.706362398956657	25.62965796332429	29.163555423532593
46	21.446519510800677	24.889673792515516	29.155010101791056	24.508796594892747
47	17.172027499659205	24.829449620241835	31.253903889545615	26.74461899055335
48	18.66847610463543	21.101858200558702	32.57619069902766	27.653474995778204
49	19.510393756218246	19.459732205893424	34.93368219948444	26.096191838403897
50	19.39910111353681	21.59321420069705	30.311476977479007	28.69620770828713
51	17.233675892223147	23.051615777919295	27.119799307015096	32.59490902284246
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	123.0
1	231.5
2	340.0
3	571.5
4	803.0
5	640.5
6	478.0
7	579.0
8	680.0
9	752.0
10	824.0
11	889.5
12	955.0
13	978.5
14	1002.0
15	1010.0
16	1018.0
17	1014.0
18	1010.0
19	1121.5
20	1233.0
21	1136.5
22	1040.0
23	1740.0
24	2440.0
25	2281.0
26	2509.0
27	2896.0
28	4426.0
29	5956.0
30	6758.0
31	7560.0
32	8332.0
33	9104.0
34	9309.5
35	9515.0
36	10753.0
37	11991.0
38	14812.5
39	17634.0
40	21542.5
41	25451.0
42	30156.5
43	34862.0
44	40552.0
45	46242.0
46	52283.0
47	58324.0
48	63060.5
49	67797.0
50	63738.5
51	59680.0
52	47205.0
53	34730.0
54	29288.0
55	23846.0
56	19855.0
57	15864.0
58	14886.0
59	13908.0
60	13132.0
61	12356.0
62	10442.5
63	8529.0
64	6515.0
65	4501.0
66	3685.5
67	2870.0
68	2171.5
69	1473.0
70	1099.0
71	725.0
72	723.0
73	721.0
74	526.5
75	255.0
76	178.0
77	185.5
78	193.0
79	125.0
80	57.0
81	46.5
82	36.0
83	54.5
84	73.0
85	49.0
86	25.0
87	12.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	491497.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.92343453578234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.65622994640096	17.02034978881797
2	12.999098676443378	6.739625671259095
3	5.917616338164654	4.602148192508623
4	3.3572728578799156	3.4812817260003555
5	2.2185301838971347	2.8755960993957266
6	1.5038876677329707	2.3391560102187627
7	1.1003569136213542	1.9967521291381074
8	0.8107779637244452	1.6814519572532451
9	0.6345922130552848	1.4805728722850322
>10	4.3846486554402295	24.11143130877581
>50	1.2204030420692442	21.948195368043972
>100	0.1863630934087699	7.859724796189018
>500	0.006290737330253836	1.054703843542858
>1k	0.003931710831408647	2.8090102365714307
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC	4060	0.8260477683485352	No Hit
CTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGCT	3433	0.698478322349882	No Hit
TCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC	3139	0.6386610701591261	No Hit
GCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC	2131	0.43357334836224837	No Hit
TCCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTG	1017	0.2069188621700641	No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	828	0.16846491433314953	No Hit
TTCCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCT	799	0.16256457313066	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTG	660	0.13428362736700325	No Hit
GCCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTG	653	0.13285940707674715	No Hit
TTTATTGGGAGAGACAGGCAGACAGCTGTGAATTCAAGGCCAGCTTGGTCT	614	0.12492446545960606	No Hit
GCCTTTGGGGATCACATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCA	556	0.11312378305462698	No Hit
TGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGCTT	540	0.10986842239118447	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCC	524	0.10661306172774197	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7247246676988873	0.0
2	0.0	0.0	0.0	2.739589458328332	0.0
3	0.0	0.0	0.0	3.7041935149146386	0.0
4	0.0	0.0	0.0	4.827292943802302	0.0
5	0.0	0.0	0.0	6.571352419241623	0.0
6	0.0	0.0	0.0	7.7050317702854745	0.0
7	0.0	0.0	0.0	8.744305662089493	0.0
8	0.0	0.0	0.0	10.352250369788626	0.0
9	0.0	0.0	0.0	11.042793750521366	0.0
10	0.0	0.0	0.0	12.475559362518998	0.0
11	0.0	0.0	0.0	15.068454130950952	0.0
12	0.0	0.0	0.0	17.386677843404943	0.0
13	2.0346004146515646E-4	0.0	0.0	18.15107721918954	0.0
14	2.0346004146515646E-4	0.0	0.0	18.38159744616956	0.0
15	2.0346004146515646E-4	0.0	0.0	18.782617187897383	0.0
16	2.0346004146515646E-4	0.0	0.0	19.761870367469182	0.0
17	2.0346004146515646E-4	0.0	0.0	21.217220044069446	0.0
18	2.0346004146515646E-4	0.0	0.0	23.105736148949028	0.0
19	2.0346004146515646E-4	0.0	0.0	24.03351393803014	0.0
20	2.0346004146515646E-4	0.0	0.0	25.069532469170717	0.0
21	2.0346004146515646E-4	0.0	0.0	26.424372885287195	0.0
22	2.0346004146515646E-4	0.0	0.0	27.685825142371165	0.0
23	2.0346004146515646E-4	0.0	0.0	28.951143140242973	0.0
24	2.0346004146515646E-4	0.0	0.0	29.774749388093927	0.0
25	2.0346004146515646E-4	0.0	0.0	30.563970888937266	0.0
26	2.0346004146515646E-4	0.0	0.0	31.241696287057703	0.0
27	2.0346004146515646E-4	0.0	0.0	31.910469443353673	0.0
28	2.0346004146515646E-4	0.0	0.0	32.58900868163997	0.0
29	2.0346004146515646E-4	0.0	0.0	33.314140269421785	0.0
30	2.0346004146515646E-4	0.0	0.0	34.141408798019114	0.0
31	2.0346004146515646E-4	0.0	0.0	34.92472995765996	0.0
32	2.0346004146515646E-4	0.0	0.0	35.6561688067272	0.0
33	2.0346004146515646E-4	0.0	0.0	36.34772948766727	0.0
34	2.0346004146515646E-4	0.0	0.0	37.033593287446315	0.0
35	2.0346004146515646E-4	0.0	0.0	37.7629975360989	0.0
36	2.0346004146515646E-4	0.0	0.0	38.465341599236616	0.0
37	2.0346004146515646E-4	0.0	0.0	39.16605798204262	0.0
38	2.0346004146515646E-4	0.0	0.0	39.807160572699324	0.0
39	2.0346004146515646E-4	0.0	0.0	40.49058285198078	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGATCGT	35	1.2100281E-7	45.000004	32
ACTGAAG	35	1.2100281E-7	45.000004	10
TTCGTAC	35	1.2100281E-7	45.000004	33
CACCTAA	35	1.2100281E-7	45.000004	45
ACGGAAT	35	1.2100281E-7	45.000004	5
AATTCGT	35	1.2100281E-7	45.000004	31
TCGTACA	35	1.2100281E-7	45.000004	34
CCGAATC	35	1.2100281E-7	45.000004	29
TAGGCCC	35	1.2100281E-7	45.000004	29
ATTGCGG	35	1.2100281E-7	45.000004	2
TTTAGTC	25	3.8875365E-5	45.0	22
GCCCCGT	25	3.8875365E-5	45.0	32
CGGGTAG	40	6.8012014E-9	45.0	6
GGAATCG	25	3.8875365E-5	45.0	8
AACCGAA	20	7.029341E-4	45.0	10
TTGGCAC	20	7.029341E-4	45.0	12
TCGCAGT	45	3.8380676E-10	45.0	41
CTCCGTC	25	3.8875365E-5	45.0	29
GTTTCGT	20	7.029341E-4	45.0	21
TACCACC	20	7.029341E-4	45.0	29
>>END_MODULE