SRR2935301.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935301.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1088883
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGC	5463	0.5017067949449114	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGCT	5368	0.4929822579652726	TruSeq Adapter, Index 16 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGC	5319	0.48848223362840637	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGC	3687	0.3386038720413488	TruSeq Adapter, Index 13 (95% over 21bp)
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	1810	0.16622538876995968	No Hit
TCCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTG	1809	0.16613355153859505	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	1467	0.1347252184118955	No Hit
TTCCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCT	1315	0.12076595924447346	No Hit
GCCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTG	1163	0.10680670007705143	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCC	1142	0.10487811821839445	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCGAA	20	7.033124E-4	45.0	29
TCGCACA	20	7.033124E-4	45.0	31
ATAGGTG	35	1.2118471E-7	45.0	24
GTCGACT	25	3.89067E-5	45.0	10
CAAGTCG	25	3.89067E-5	45.0	43
GGCCGTA	20	7.033124E-4	45.0	9
CACGATA	25	3.89067E-5	45.0	20
TACGGTA	25	3.89067E-5	45.0	13
CACGAAA	25	3.89067E-5	45.0	35
CTAACCG	20	7.033124E-4	45.0	15
TAACCGG	20	7.033124E-4	45.0	16
CGTAGTG	20	7.033124E-4	45.0	12
CTACGCG	40	6.8139343E-9	45.0	43
AGGCACG	40	6.8139343E-9	45.0	10
TGCGAAC	25	3.89067E-5	45.0	10
TCGCTAA	20	7.033124E-4	45.0	20
GGAACGT	20	7.033124E-4	45.0	8
ACTACGC	40	6.8139343E-9	45.0	42
CGCAGAT	20	7.033124E-4	45.0	24
GCGATAG	20	7.033124E-4	45.0	9