##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935301.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1088883
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30424940053247	33.0	31.0	34.0	30.0	34.0
2	32.45837064220858	34.0	31.0	34.0	30.0	34.0
3	32.518472599902836	34.0	31.0	34.0	30.0	34.0
4	35.959288555336066	37.0	35.0	37.0	35.0	37.0
5	31.84131536629739	37.0	35.0	37.0	0.0	37.0
6	33.51213215744942	37.0	35.0	37.0	17.0	37.0
7	35.24506673352417	37.0	35.0	37.0	32.0	37.0
8	35.77411439061864	37.0	35.0	37.0	35.0	37.0
9	37.82439251967383	39.0	38.0	39.0	35.0	39.0
10	37.53976689873935	39.0	37.0	39.0	35.0	39.0
11	37.4177840961793	39.0	37.0	39.0	35.0	39.0
12	37.08236330257704	39.0	37.0	39.0	34.0	39.0
13	36.92093640914589	39.0	35.0	39.0	33.0	39.0
14	37.95825997834478	40.0	37.0	41.0	33.0	41.0
15	38.12320607448183	40.0	37.0	41.0	33.0	41.0
16	38.248888999093566	40.0	37.0	41.0	34.0	41.0
17	38.26843563541721	40.0	37.0	41.0	34.0	41.0
18	38.2634415267756	40.0	37.0	41.0	34.0	41.0
19	38.288524111405906	40.0	37.0	41.0	34.0	41.0
20	38.20303099598396	40.0	37.0	41.0	34.0	41.0
21	38.15014744467496	40.0	36.0	41.0	34.0	41.0
22	38.14932182796499	40.0	36.0	41.0	34.0	41.0
23	38.110506822128734	40.0	36.0	41.0	34.0	41.0
24	38.063953611177695	40.0	36.0	41.0	34.0	41.0
25	37.976153544503866	40.0	36.0	41.0	34.0	41.0
26	37.899018535508404	40.0	36.0	41.0	34.0	41.0
27	37.870375421418096	40.0	36.0	41.0	34.0	41.0
28	37.8450981418573	40.0	36.0	41.0	34.0	41.0
29	37.798003091241206	40.0	36.0	41.0	34.0	41.0
30	37.7398499195965	40.0	36.0	41.0	34.0	41.0
31	37.53628718604295	40.0	35.0	41.0	33.0	41.0
32	37.55658596929147	39.0	35.0	41.0	33.0	41.0
33	37.58395989284432	40.0	35.0	41.0	33.0	41.0
34	37.51123582607131	40.0	35.0	41.0	33.0	41.0
35	37.42144748333843	39.0	35.0	41.0	33.0	41.0
36	37.41963920825286	39.0	35.0	41.0	33.0	41.0
37	37.41497938713342	39.0	35.0	41.0	33.0	41.0
38	37.410416913479224	39.0	35.0	41.0	33.0	41.0
39	37.39423060145121	39.0	35.0	41.0	33.0	41.0
40	37.26917676187433	39.0	35.0	41.0	33.0	41.0
41	37.215983719095625	39.0	35.0	41.0	33.0	41.0
42	37.21874434627044	39.0	35.0	41.0	33.0	41.0
43	37.18531100219216	39.0	35.0	41.0	33.0	41.0
44	37.13193795843998	39.0	35.0	41.0	33.0	41.0
45	37.111817339420305	39.0	35.0	41.0	33.0	41.0
46	37.00026081773708	39.0	35.0	41.0	33.0	41.0
47	36.9568006847384	39.0	35.0	41.0	32.0	41.0
48	36.94333091801415	39.0	35.0	41.0	32.0	41.0
49	36.942622852960326	39.0	35.0	41.0	32.0	41.0
50	36.84697529486639	39.0	35.0	41.0	32.0	41.0
51	36.19915546482037	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	12.0
11	7.0
12	21.0
13	18.0
14	21.0
15	27.0
16	55.0
17	92.0
18	139.0
19	240.0
20	374.0
21	649.0
22	1004.0
23	1655.0
24	2524.0
25	3546.0
26	5063.0
27	6391.0
28	8223.0
29	10792.0
30	14276.0
31	19188.0
32	26766.0
33	42726.0
34	75007.0
35	102038.0
36	86954.0
37	136049.0
38	221264.0
39	323367.0
40	387.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.76314351495983	4.902730596400164	55.31071749673748	17.023408391902528
2	18.668396880105576	5.012292413418154	53.92884267639406	22.390468030082204
3	20.786255272605043	4.513983596033733	52.62824380580834	22.071517325552882
4	19.048786692417828	4.6746987509218165	50.90436713586308	25.372147420797276
5	15.857993925885516	16.769478447179356	44.308249830330716	23.06427779660441
6	21.290349835565436	6.181104856995655	53.04013378847865	19.488411518960255
7	82.54853827270698	2.317053347329327	9.51479635553131	5.619612024432377
8	83.66711575072804	2.7759639924583266	7.599898244347648	5.957022012465986
9	78.93391668342696	4.819617902015184	10.071972838220452	6.174492576337403
10	41.16172260931615	25.571342375627133	17.389471596121897	15.877463418934818
11	31.01324935736897	24.770797229821753	23.745801890561243	20.470151522248027
12	28.932401369109446	20.80407169548978	29.660762451062233	20.602764484338536
13	24.25118217476074	25.7328840655975	30.066958525387943	19.94897523425382
14	18.655355993251803	27.823191288687582	31.412465802110972	22.108986915949647
15	16.669835051148745	24.572428810074175	37.18406844445179	21.573667694325284
16	19.731963856539224	23.26530949606156	33.56586520314855	23.436861444250667
17	19.704137175435747	23.56616826601205	28.91081961973876	27.818874938813444
18	20.87166389777414	22.50333598742932	32.74300361012156	23.881996504674973
19	23.358616123128012	24.185243042640945	29.45670012297005	22.99944071126099
20	23.893384321364188	26.64877677399684	28.868850005005132	20.588988899633843
21	22.13460950350038	27.271616877111683	29.79778360025825	20.795990019129697
22	21.649617084663824	24.44642812864192	28.527307341560114	25.376647445134143
23	20.977368551074818	25.286279609471357	28.34620432130908	25.39014751814474
24	21.760280948458192	24.65342924813777	29.849304287053798	23.73698551635024
25	20.833092260601003	26.640511423174022	26.87864536410248	25.647750952122493
26	18.61393740190636	29.143627001248067	25.866690911695745	26.375744685149826
27	19.422931572997282	26.687164736707246	29.117269715846422	24.772633974449047
28	16.79996840799241	25.711945176846363	32.009315968749625	25.4787704464116
29	18.442844639874075	24.985237165058138	29.71439539417917	26.857522800888617
30	22.373937328436572	25.43597429659569	28.082815141755358	24.107273233212386
31	22.03827224779889	26.264897146892736	26.54839868011531	25.148431925193066
32	22.29973284549396	26.017671320059183	26.649511471847752	25.033084362599105
33	23.1663089606505	24.503367212087984	26.28813196642798	26.042191860833537
34	19.332196388409038	24.26762103917501	29.394526317336204	27.005656255079746
35	18.31179291071676	25.35056567142659	28.80805375784175	27.529587660014897
36	21.904924587857465	25.196279122734033	28.390745378520926	24.50805091088758
37	19.02656208242759	25.610740547882553	30.49694044263709	24.865756927052768
38	19.621391829976222	26.040355116206243	28.097876447699154	26.24037660611838
39	21.433248567568782	23.465606497667792	29.046463210464303	26.054681724299122
40	22.99925703679826	22.34601881010173	28.933227904191728	25.721496248908288
41	18.4684672274248	22.988512080728597	28.633746692711703	29.909273999134893
42	19.035837642795414	23.34484053842332	30.520726285560524	27.09859553322074
43	20.163323332258837	22.917430063652386	30.7190947053081	26.20015189878068
44	19.973220263334078	23.56809684787071	28.21744852293589	28.241234365859324
45	20.155333493130115	24.245947452572956	25.42091299065189	30.17780606364504
46	21.70416840009441	23.760312173116855	28.794094498674326	25.74142492811441
47	17.91726016477436	23.639454376641016	32.141194232989214	26.302091225595404
48	17.96455633892714	23.208737761540952	31.85512125728843	26.971584642243474
49	21.306145839360152	20.527549791850916	32.398613992504245	25.767690376284687
50	20.24496663094198	21.47347327490649	29.70080348393721	28.58075661021432
51	18.853448901305285	21.876271371671706	28.17437686142588	31.09590286559713
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	300.0
1	597.0
2	894.0
3	1328.0
4	1762.0
5	1372.0
6	982.0
7	1086.5
8	1191.0
9	1472.5
10	1754.0
11	1977.5
12	2201.0
13	2289.0
14	2377.0
15	2449.0
16	2521.0
17	2515.5
18	2510.0
19	2442.5
20	2375.0
21	2560.5
22	2746.0
23	3410.5
24	4075.0
25	4544.5
26	8060.5
27	11107.0
28	11104.5
29	11102.0
30	13315.0
31	15528.0
32	19217.5
33	22907.0
34	24544.0
35	26181.0
36	28583.5
37	30986.0
38	34315.5
39	37645.0
40	42520.5
41	47396.0
42	55191.0
43	62986.0
44	71775.0
45	80564.0
46	93273.5
47	105983.0
48	119834.5
49	133686.0
50	127184.0
51	120682.0
52	101394.0
53	82106.0
54	70989.5
55	59873.0
56	54188.5
57	48504.0
58	45364.0
59	42224.0
60	37991.5
61	33759.0
62	29165.5
63	24572.0
64	22540.0
65	20508.0
66	17361.5
67	14215.0
68	11803.0
69	9391.0
70	8062.5
71	6734.0
72	5348.0
73	3962.0
74	3427.0
75	2046.0
76	1200.0
77	996.0
78	792.0
79	597.5
80	403.0
81	309.5
82	216.0
83	143.5
84	71.0
85	37.0
86	3.0
87	2.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1088883.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.015447534155342
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.75683612071252	16.449366839897355
2	12.912419980006314	6.460199290976543
3	5.867956232633822	4.403686538105137
4	3.3820072225300466	3.384096969413393
5	2.094893488709341	2.6202349078226086
6	1.4762495424575224	2.215742578600019
7	1.0681539103343851	1.8704243671660876
8	0.7902064642703436	1.5813894678484146
9	0.6392160967262079	1.4391249057577822
>10	4.4095929932535025	23.26756997110008
>50	1.366897807521582	24.088817450930144
>100	0.22301624619119215	8.111040043750759
>500	0.008861572696335385	1.4719300135190678
>1k	0.002584625369764487	1.144680513148959
>5k	0.0011076965870419231	1.4916961419636638
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGC	5463	0.5017067949449114	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGCT	5368	0.4929822579652726	TruSeq Adapter, Index 16 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGC	5319	0.48848223362840637	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGC	3687	0.3386038720413488	TruSeq Adapter, Index 13 (95% over 21bp)
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	1810	0.16622538876995968	No Hit
TCCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTG	1809	0.16613355153859505	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	1467	0.1347252184118955	No Hit
TTCCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCT	1315	0.12076595924447346	No Hit
GCCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTG	1163	0.10680670007705143	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCC	1142	0.10487811821839445	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.183723136461861E-5	0.0	0.0	0.5078598894463409	0.0
2	9.183723136461861E-5	0.0	0.0	1.9433676529066943	0.0
3	9.183723136461861E-5	0.0	0.0	2.7088309763307903	0.0
4	9.183723136461861E-5	0.0	0.0	3.5791724179732807	0.0
5	9.183723136461861E-5	0.0	0.0	5.07584377752247	0.0
6	9.183723136461861E-5	0.0	0.0	6.071726714440395	0.0
7	9.183723136461861E-5	0.0	0.0	6.960619276818538	0.0
8	9.183723136461861E-5	0.0	0.0	8.314667416058475	0.0
9	9.183723136461861E-5	0.0	0.0	8.933283006530546	0.0
10	9.183723136461861E-5	0.0	0.0	10.106595474444912	0.0
11	9.183723136461861E-5	0.0	0.0	12.525771823051697	0.0
12	9.183723136461861E-5	0.0	0.0	14.56712980182444	0.0
13	9.183723136461861E-5	0.0	0.0	15.20962307245131	0.0
14	9.183723136461861E-5	0.0	0.0	15.424154844919059	0.0
15	9.183723136461861E-5	0.0	0.0	15.77331999856734	0.0
16	9.183723136461861E-5	0.0	0.0	16.574967191149096	0.0
17	9.183723136461861E-5	0.0	0.0	17.82119842076697	0.0
18	9.183723136461861E-5	0.0	0.0	19.22842031696702	0.0
19	9.183723136461861E-5	0.0	0.0	20.08562903452437	0.0
20	9.183723136461861E-5	0.0	0.0	21.00666462788013	0.0
21	9.183723136461861E-5	0.0	0.0	22.283936841699244	0.0
22	9.183723136461861E-5	0.0	0.0	23.50206587851955	0.0
23	9.183723136461861E-5	0.0	0.0	24.6639905297447	0.0
24	1.8367446272923721E-4	0.0	0.0	25.54617897423323	0.0
25	1.8367446272923721E-4	0.0	0.0	26.3306526045498	0.0
26	1.8367446272923721E-4	0.0	0.0	27.038809495602376	0.0
27	1.8367446272923721E-4	0.0	0.0	27.739343896451686	0.0
28	1.8367446272923721E-4	0.0	0.0	28.45806206911119	0.0
29	1.8367446272923721E-4	0.0	0.0	29.24923981731738	0.0
30	1.8367446272923721E-4	0.0	0.0	30.087438227982254	0.0
31	2.7551169409385584E-4	0.0	0.0	30.82939122017701	0.0
32	2.7551169409385584E-4	0.0	0.0	31.489058053069062	0.0
33	2.7551169409385584E-4	0.0	0.0	32.19932720044302	0.0
34	2.7551169409385584E-4	0.0	0.0	32.9269535845449	0.0
35	2.7551169409385584E-4	0.0	0.0	33.74347840860772	0.0
36	2.7551169409385584E-4	0.0	0.0	34.396900309766984	0.0
37	2.7551169409385584E-4	0.0	0.0	35.085404033307526	0.0
38	2.7551169409385584E-4	0.0	0.0	35.78575475969411	0.0
39	2.7551169409385584E-4	0.0	0.0	36.74021910526659	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCGAA	20	7.033124E-4	45.0	29
TCGCACA	20	7.033124E-4	45.0	31
ATAGGTG	35	1.2118471E-7	45.0	24
GTCGACT	25	3.89067E-5	45.0	10
CAAGTCG	25	3.89067E-5	45.0	43
GGCCGTA	20	7.033124E-4	45.0	9
CACGATA	25	3.89067E-5	45.0	20
TACGGTA	25	3.89067E-5	45.0	13
CACGAAA	25	3.89067E-5	45.0	35
CTAACCG	20	7.033124E-4	45.0	15
TAACCGG	20	7.033124E-4	45.0	16
CGTAGTG	20	7.033124E-4	45.0	12
CTACGCG	40	6.8139343E-9	45.0	43
AGGCACG	40	6.8139343E-9	45.0	10
TGCGAAC	25	3.89067E-5	45.0	10
TCGCTAA	20	7.033124E-4	45.0	20
GGAACGT	20	7.033124E-4	45.0	8
ACTACGC	40	6.8139343E-9	45.0	42
CGCAGAT	20	7.033124E-4	45.0	24
GCGATAG	20	7.033124E-4	45.0	9
>>END_MODULE