FastQCFastQC Report
Sat 14 Jan 2017
SRR2935300.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935300.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2657748
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163920.6167627630610577No Hit
CCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC98750.37155516625353496No Hit
CTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGCT90260.3396108284156361Illumina Single End Adapter 1 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTT86650.3260279003125955No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTC83260.3132727406812083No Hit
TCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC75640.2846018508903026No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCT73800.27767869640011017No Hit
CGCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTG65390.24603536527917622No Hit
GCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC55240.2078451380642559No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCC51780.19482659755552445No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTC42050.15821665560466983No Hit
CGTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCT40540.15253515382195754No Hit
TCCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTG33770.12706246039880378No Hit
TTCCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCT28050.10554048013581424No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGT26780.10076199850399663No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCACG302.166582E-645.0000041
TCGCTAG207.034959E-445.01
AACGCGC253.8921928E-545.042
TAAGCGT207.034959E-445.010
CGTTTTT117850.043.854481
CGTTATT18350.043.4059941
CCTACGG1400.043.3928572
CTTACGG4950.041.818182
CGTATGG3750.041.3999982
CTACGGG7800.041.2500043
TTCGCGC556.184564E-1140.9090921
TTATGGG58500.040.8846173
TTTACGG9250.040.621622
CCGGCTA501.0822987E-940.519
TAGTTCG501.0822987E-940.51
CTTCGCG1950.040.3846171
TATGGGA37700.040.3448264
TATGGGT13550.040.18454
TCACGGG7500.039.8999983
TTGTACG2200.039.8863641
Produced by FastQC (version 0.11.5)