##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935300.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2657748
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31058512695711	33.0	31.0	34.0	30.0	34.0
2	32.47845807804201	34.0	31.0	34.0	31.0	34.0
3	32.47859879868219	34.0	31.0	34.0	30.0	34.0
4	35.99534982248129	37.0	35.0	37.0	35.0	37.0
5	31.733741874699934	37.0	35.0	37.0	0.0	37.0
6	33.52811967124046	37.0	35.0	37.0	17.0	37.0
7	35.35322686725754	37.0	35.0	37.0	32.0	37.0
8	35.88581874579531	37.0	35.0	37.0	35.0	37.0
9	37.95703053863647	39.0	38.0	39.0	35.0	39.0
10	37.59344640650656	39.0	37.0	39.0	35.0	39.0
11	37.44977514798243	39.0	37.0	39.0	35.0	39.0
12	37.37122932648242	39.0	37.0	39.0	35.0	39.0
13	37.38497818453819	39.0	37.0	39.0	35.0	39.0
14	38.619057186761125	40.0	38.0	41.0	35.0	41.0
15	38.697968355163844	40.0	38.0	41.0	35.0	41.0
16	38.73398079878152	40.0	38.0	41.0	35.0	41.0
17	38.72970424585024	40.0	38.0	41.0	35.0	41.0
18	38.64306886883181	40.0	38.0	41.0	35.0	41.0
19	38.595085011822036	40.0	38.0	41.0	35.0	41.0
20	38.45649738048905	40.0	37.0	41.0	34.0	41.0
21	38.407003598535304	40.0	37.0	41.0	34.0	41.0
22	38.382798519648965	40.0	37.0	41.0	34.0	41.0
23	38.32227509906883	40.0	37.0	41.0	34.0	41.0
24	38.303955830274354	40.0	37.0	41.0	34.0	41.0
25	38.216093474625886	40.0	37.0	41.0	34.0	41.0
26	38.13705663591883	40.0	37.0	41.0	34.0	41.0
27	38.09509291324836	40.0	37.0	41.0	34.0	41.0
28	38.05640226236648	40.0	37.0	41.0	34.0	41.0
29	37.986785805125244	40.0	37.0	41.0	34.0	41.0
30	37.90873137709068	40.0	36.0	41.0	34.0	41.0
31	37.6255657044987	40.0	36.0	41.0	33.0	41.0
32	37.524832677891204	40.0	36.0	41.0	33.0	41.0
33	37.396460085756814	40.0	36.0	41.0	33.0	41.0
34	37.26390726284057	40.0	36.0	41.0	33.0	41.0
35	37.132223596819564	40.0	36.0	41.0	32.0	41.0
36	37.074890094922466	40.0	36.0	41.0	32.0	41.0
37	37.01873729187267	40.0	36.0	41.0	32.0	41.0
38	37.01620018150705	40.0	36.0	41.0	32.0	41.0
39	37.009711605464474	40.0	36.0	41.0	32.0	41.0
40	36.980461277743416	40.0	36.0	41.0	31.0	41.0
41	36.914220987091326	40.0	36.0	41.0	31.0	41.0
42	36.83938883596188	40.0	35.0	41.0	31.0	41.0
43	36.80203126857776	39.0	35.0	41.0	31.0	41.0
44	36.78333724642065	39.0	35.0	41.0	31.0	41.0
45	36.78474181901369	39.0	35.0	41.0	31.0	41.0
46	36.70629231966311	39.0	35.0	41.0	31.0	41.0
47	36.64445199469626	39.0	35.0	41.0	31.0	41.0
48	36.5874819584099	39.0	35.0	41.0	31.0	41.0
49	36.563029301498865	39.0	35.0	41.0	31.0	41.0
50	36.51828277173005	39.0	35.0	41.0	31.0	41.0
51	35.893321338215664	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	11.0
11	12.0
12	16.0
13	14.0
14	18.0
15	40.0
16	100.0
17	180.0
18	350.0
19	652.0
20	1235.0
21	2218.0
22	3694.0
23	6470.0
24	11583.0
25	20098.0
26	26946.0
27	28526.0
28	27020.0
29	27857.0
30	33080.0
31	41835.0
32	55603.0
33	80097.0
34	126996.0
35	183430.0
36	215006.0
37	339403.0
38	570366.0
39	854055.0
40	830.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.728433244987862	4.20626786286736	57.26615164417394	16.79914724797084
2	23.604419982631914	4.136923440446574	52.04199194205019	20.216664634871325
3	18.075227598703865	4.084284890817338	56.04850422237172	21.791983288107076
4	16.31595621556295	3.710208793309223	56.26519143274682	23.708643558381006
5	14.522407692527658	16.324121022760625	48.5529102081913	20.600561076520425
6	18.87880265548126	5.726013150983464	57.852399851302685	17.542784342232597
7	80.59785954123566	1.3608889932378843	13.29001094159416	4.7512405239322915
8	83.19272557067112	1.6693832522872747	10.743080231835373	4.394810945206242
9	78.5066153751221	3.7784244405413907	12.607478210876275	5.107481973460238
10	35.949909472229876	26.875572853408226	21.700891130385575	15.47362654397633
11	22.93994765493192	25.845753622992095	30.257496196027617	20.956802526048367
12	20.018903221825397	22.10081617971305	36.118285104532106	21.761995493929447
13	20.806336793405546	23.912387479926615	36.95549766193032	18.325778064737513
14	19.222815707132504	26.378836518736914	35.02943093175124	19.36891684237934
15	17.087737437860927	26.359120578775713	36.40864370888436	20.144498274478995
16	18.208310193441967	26.761039797603082	34.47693310276219	20.55371690619276
17	19.34446004662594	26.656515215137027	31.400004816107476	22.599019922129564
18	19.507831442258635	25.739949761978938	33.938582589470485	20.813636206291942
19	19.967581576582884	28.700727081724832	30.895969068549768	20.435722273142527
20	20.7827077661238	28.64774989953901	31.099186228340685	19.470356105996505
21	20.614934147255497	28.38455715139283	32.05680147252486	18.94370722882681
22	20.101905824028464	25.54497266106493	31.31538430280072	23.03773721210589
23	18.65594480740838	27.35914014421232	30.329624930580323	23.65529011779898
24	19.664881696835064	26.92087436431144	31.572368787409495	21.841875151444004
25	19.255362058404334	28.96749428463496	29.174135395831357	22.603008261129347
26	18.823868929635164	30.217086044275078	29.66255641994651	21.296488606143246
27	20.194992151249856	27.901253241466083	30.607228375301194	21.296526231982867
28	18.87959279811329	26.645415592448945	32.125694384870194	22.349297224567565
29	19.31368210981628	25.73690206896967	31.55543715958022	23.393978661633835
30	21.61318529822993	26.8704181133802	30.29207434263896	21.224322245750916
31	21.35089557023465	28.099353287068602	29.61638951473202	20.93336162796473
32	21.319703749189163	28.652904639567033	28.598798682192594	21.428592929051213
33	21.948393903409954	27.391836998842628	27.95938516367993	22.70038393406749
34	19.91524403367061	26.930807585971284	29.92593729729079	23.22801108306732
35	21.175258150885636	27.696493422250718	28.54510660905398	22.583141817809665
36	22.354715345472933	28.530056273205734	27.688817750968113	21.426410630353214
37	20.825450719932814	28.350581018215422	30.95094042023548	19.873027841616285
38	21.435591335220646	27.810311587103065	29.252020883846026	21.502076193830266
39	20.882228111920316	27.076570088661526	29.066036358601345	22.97516544081681
40	21.083940238126413	25.835782775492632	30.334591541410244	22.745685444970704
41	20.607333727652133	27.54501179193814	28.84174872862288	23.00590575178685
42	21.133907353142586	29.02344390815081	28.364427327195806	21.4782214115108
43	20.894381258117775	27.462234944772796	29.718506043462362	21.924877753647074
44	20.630812251575396	26.358480939502165	28.391555557562263	24.619151251360176
45	21.32820718894342	25.548358986630788	27.440374331953215	25.683059492472577
46	22.349184347048702	25.734042505158506	28.392571455232023	23.52420169256077
47	19.947639881583957	26.652451624458003	30.840696710147085	22.559211783810955
48	20.47560566314037	25.9321049249214	30.439304253074408	23.15298515886382
49	21.382877533912172	24.368676037005766	30.989845538403188	23.258600890678878
50	20.741808478456196	23.821370573884355	30.47162484930851	24.965196098350933
51	19.52536508352184	23.70905744261683	28.980512825143695	27.785064648717633
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	558.0
1	823.5
2	1089.0
3	14798.0
4	28507.0
5	19411.5
6	10316.0
7	10950.5
8	11585.0
9	12726.0
10	13867.0
11	15245.0
12	16623.0
13	17099.5
14	17576.0
15	17097.0
16	16618.0
17	15894.5
18	15171.0
19	14387.0
20	13603.0
21	13081.0
22	12559.0
23	13680.5
24	14802.0
25	15856.5
26	20467.5
27	24024.0
28	27749.5
29	31475.0
30	36687.0
31	41899.0
32	48240.0
33	54581.0
34	64366.5
35	74152.0
36	80697.5
37	87243.0
38	99974.5
39	112706.0
40	127466.0
41	142226.0
42	157326.0
43	172426.0
44	196398.5
45	220371.0
46	264635.5
47	308900.0
48	324647.0
49	340394.0
50	317674.0
51	294954.0
52	240380.0
53	185806.0
54	156185.5
55	126565.0
56	106298.5
57	86032.0
58	74227.0
59	62422.0
60	50387.0
61	38352.0
62	32488.0
63	26624.0
64	22077.0
65	17530.0
66	13240.0
67	8950.0
68	6623.0
69	4296.0
70	3560.5
71	2825.0
72	2075.5
73	1326.0
74	1083.0
75	614.0
76	388.0
77	335.0
78	282.0
79	208.0
80	134.0
81	176.0
82	218.0
83	118.5
84	19.0
85	11.0
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2657748.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.0052630616682
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.92268770147217	22.378940138644886
2	12.761123098859766	8.168462034826748
3	5.312717721006777	5.1010478469962495
4	2.806245944342685	3.5925855865770857
5	1.6621351402081437	2.6598536203202197
6	1.0817831502655457	2.077365257995736
7	0.7363297897273537	1.6496500034256754
8	0.5854491128795256	1.49899622935436
9	0.4385565571710182	1.263250618171017
>10	4.0665434130139975	28.153533759437032
>50	0.5073340221416567	10.815780795213005
>100	0.10953468536090928	6.374085242157148
>500	0.0054626648860882305	1.2348050485535702
>1k	0.0028554839177279377	1.675267590811786
>5k	0.0011173632721544102	2.7050400080570665
>10k+	1.241514746838234E-4	0.6513362194584283
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16392	0.6167627630610577	No Hit
CCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC	9875	0.37155516625353496	No Hit
CTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGCT	9026	0.3396108284156361	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTT	8665	0.3260279003125955	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTC	8326	0.3132727406812083	No Hit
TCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC	7564	0.2846018508903026	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCT	7380	0.27767869640011017	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTG	6539	0.24603536527917622	No Hit
GCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC	5524	0.2078451380642559	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCC	5178	0.19482659755552445	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTC	4205	0.15821665560466983	No Hit
CGTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCT	4054	0.15253515382195754	No Hit
TCCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTG	3377	0.12706246039880378	No Hit
TTCCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCT	2805	0.10554048013581424	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGT	2678	0.10076199850399663	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.525167924122227E-5	0.0	0.0	0.3505599477452339	0.0
2	7.525167924122227E-5	0.0	0.0	1.2694205771201785	0.0
3	7.525167924122227E-5	0.0	0.0	1.9904445417699497	0.0
4	1.128775188618334E-4	0.0	0.0	2.8204705638006313	0.0
5	1.128775188618334E-4	0.0	0.0	4.446207842127997	0.0
6	1.128775188618334E-4	0.0	0.0	6.101443778717922	0.0
7	1.128775188618334E-4	0.0	0.0	7.424631680655954	0.0
8	1.128775188618334E-4	0.0	0.0	9.10274412773521	0.0
9	1.128775188618334E-4	0.0	0.0	9.937887263954295	0.0
10	1.128775188618334E-4	0.0	0.0	11.250013169043868	0.0
11	1.128775188618334E-4	0.0	0.0	13.671950839582985	0.0
12	1.128775188618334E-4	0.0	0.0	15.602231663799577	0.0
13	1.5050335848244454E-4	0.0	0.0	16.319982180402356	0.0
14	1.5050335848244454E-4	0.0	0.0	16.585883989001214	0.0
15	1.5050335848244454E-4	0.0	0.0	16.87496331480637	0.0
16	1.5050335848244454E-4	0.0	0.0	17.550083755118994	0.0
17	1.5050335848244454E-4	0.0	0.0	18.64559770151271	0.0
18	1.5050335848244454E-4	0.0	0.0	19.82520539945849	0.0
19	1.5050335848244454E-4	0.0	0.0	20.67626426583709	0.0
20	1.5050335848244454E-4	0.0	0.0	21.464638483407757	0.0
21	1.5050335848244454E-4	0.0	0.0	22.63164152508063	0.0
22	1.5050335848244454E-4	0.0	0.0	23.90275526498374	0.0
23	1.5050335848244454E-4	0.0	0.0	25.250174207637443	0.0
24	1.5050335848244454E-4	0.0	0.0	26.2165186466136	0.0
25	1.5050335848244454E-4	0.0	0.0	27.055913502709814	0.0
26	1.5050335848244454E-4	0.0	0.0	27.81298302171613	0.0
27	1.5050335848244454E-4	0.0	0.0	28.552067389383794	0.0
28	1.5050335848244454E-4	0.0	0.0	29.338146430737602	0.0
29	1.5050335848244454E-4	0.0	0.0	30.177052150918747	0.0
30	1.5050335848244454E-4	0.0	0.0	31.057873056437256	0.0
31	1.5050335848244454E-4	0.0	0.0	31.8783421152043	0.0
32	1.5050335848244454E-4	0.0	0.0	32.66047044339795	0.0
33	1.8812919810305568E-4	0.0	0.0	33.44421668269528	0.0
34	2.257550377236668E-4	0.0	0.0	34.220738760785444	0.0
35	2.257550377236668E-4	0.0	0.0	35.04959838178789	0.0
36	2.257550377236668E-4	0.0	0.0	35.81757939428418	0.0
37	2.257550377236668E-4	0.0	0.0	36.59312320054422	0.0
38	2.257550377236668E-4	0.0	0.0	37.37051067294567	0.0
39	2.257550377236668E-4	0.0	0.0	38.170774655836446	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCACG	30	2.166582E-6	45.000004	1
TCGCTAG	20	7.034959E-4	45.0	1
AACGCGC	25	3.8921928E-5	45.0	42
TAAGCGT	20	7.034959E-4	45.0	10
CGTTTTT	11785	0.0	43.85448	1
CGTTATT	1835	0.0	43.405994	1
CCTACGG	140	0.0	43.392857	2
CTTACGG	495	0.0	41.81818	2
CGTATGG	375	0.0	41.399998	2
CTACGGG	780	0.0	41.250004	3
TTCGCGC	55	6.184564E-11	40.909092	1
TTATGGG	5850	0.0	40.884617	3
TTTACGG	925	0.0	40.62162	2
CCGGCTA	50	1.0822987E-9	40.5	19
TAGTTCG	50	1.0822987E-9	40.5	1
CTTCGCG	195	0.0	40.384617	1
TATGGGA	3770	0.0	40.344826	4
TATGGGT	1355	0.0	40.1845	4
TCACGGG	750	0.0	39.899998	3
TTGTACG	220	0.0	39.886364	1
>>END_MODULE