##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935299.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	615315
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21884725709596	33.0	31.0	34.0	30.0	34.0
2	32.373800411171516	34.0	31.0	34.0	30.0	34.0
3	32.429597848256584	34.0	31.0	34.0	30.0	34.0
4	35.932423230377935	37.0	35.0	37.0	35.0	37.0
5	31.56783273607827	37.0	35.0	37.0	0.0	37.0
6	33.38627207202815	37.0	35.0	37.0	17.0	37.0
7	35.22395033438158	37.0	35.0	37.0	32.0	37.0
8	35.782291996782135	37.0	35.0	37.0	35.0	37.0
9	37.84741636397617	39.0	38.0	39.0	35.0	39.0
10	37.48618024914068	39.0	37.0	39.0	35.0	39.0
11	37.37092546094277	39.0	37.0	39.0	35.0	39.0
12	37.032828713748245	39.0	35.0	39.0	34.0	39.0
13	36.82988388061399	39.0	35.0	39.0	33.0	39.0
14	37.91498663286284	40.0	36.0	41.0	33.0	41.0
15	38.06083875738443	40.0	36.0	41.0	33.0	41.0
16	38.193835677661035	40.0	36.0	41.0	34.0	41.0
17	38.187291062301426	40.0	36.0	41.0	34.0	41.0
18	38.2103410448307	40.0	36.0	41.0	34.0	41.0
19	38.241864735948255	40.0	36.0	41.0	34.0	41.0
20	38.163610508438765	40.0	36.0	41.0	34.0	41.0
21	38.063135142162956	40.0	36.0	41.0	34.0	41.0
22	38.03323826007817	40.0	36.0	41.0	34.0	41.0
23	37.986129055849446	40.0	36.0	41.0	34.0	41.0
24	37.98013375263076	40.0	36.0	41.0	34.0	41.0
25	37.870385087313004	39.0	36.0	41.0	34.0	41.0
26	37.82515134524593	39.0	36.0	41.0	34.0	41.0
27	37.754316082006774	39.0	35.0	41.0	34.0	41.0
28	37.71125683592957	39.0	36.0	41.0	33.0	41.0
29	37.65945897629669	39.0	36.0	41.0	33.0	41.0
30	37.62854472912248	39.0	36.0	41.0	33.0	41.0
31	37.373436369989356	39.0	35.0	41.0	33.0	41.0
32	37.35268277223861	39.0	35.0	41.0	33.0	41.0
33	37.36770922210575	39.0	35.0	41.0	33.0	41.0
34	37.269749640428074	39.0	35.0	41.0	33.0	41.0
35	37.226778154278705	39.0	35.0	41.0	33.0	41.0
36	37.221571065226755	39.0	35.0	41.0	33.0	41.0
37	37.107073612702436	39.0	35.0	41.0	32.0	41.0
38	37.08690670632115	39.0	35.0	41.0	32.0	41.0
39	37.136586951398876	39.0	35.0	41.0	32.0	41.0
40	37.082312311580246	39.0	35.0	41.0	32.0	41.0
41	37.11062138904464	39.0	35.0	41.0	32.0	41.0
42	37.08677506642939	39.0	35.0	41.0	32.0	41.0
43	36.942744773002445	39.0	35.0	41.0	32.0	41.0
44	36.8172708287625	39.0	35.0	40.0	32.0	41.0
45	36.88313302942395	39.0	35.0	40.0	32.0	41.0
46	36.86414763170084	39.0	35.0	40.0	32.0	41.0
47	36.8513103044782	39.0	35.0	40.0	32.0	41.0
48	36.80289120206724	39.0	35.0	40.0	32.0	41.0
49	36.776480339338384	39.0	35.0	40.0	32.0	41.0
50	36.63127991354022	38.0	35.0	40.0	31.0	41.0
51	35.95978645084225	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	5.0
11	1.0
12	4.0
13	7.0
14	10.0
15	8.0
16	18.0
17	21.0
18	65.0
19	101.0
20	198.0
21	294.0
22	548.0
23	806.0
24	1302.0
25	1882.0
26	2720.0
27	3619.0
28	4756.0
29	6253.0
30	8582.0
31	11574.0
32	16031.0
33	25416.0
34	42428.0
35	66035.0
36	52679.0
37	82478.0
38	131753.0
39	155589.0
40	126.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.966935634593664	4.5298749421028255	57.42895915100396	15.074230272299555
2	18.302007914645344	4.291948026620512	55.66595971169238	21.740084347041762
3	19.4729528777943	4.306574681260818	55.267302113551594	20.953170327393288
4	18.688639152304106	4.263669827649253	53.08711798022151	23.96057303982513
5	14.808675231385552	16.459049429966765	46.7966813745805	21.935593964067184
6	19.51276988209291	5.984739523658614	55.490114819238926	19.01237577500955
7	81.72285739824318	1.61315748844088	10.87930572145974	5.784679391856204
8	83.65243818206935	1.8587227680131313	7.774066941322737	6.714772108594786
9	79.03951634528656	4.276671298440636	10.463746211290152	6.220066144982652
10	38.15801662563077	26.458318097234752	18.49166686981465	16.89199840731983
11	29.17692563971299	27.725798981009724	24.410423929206992	18.68685145007029
12	26.733786759627183	22.25022955721866	31.48842462803605	19.52755905511811
13	21.587804620397684	29.719086971713676	31.000056881434713	17.693051526453928
14	16.703314562459877	32.998057905300534	29.402013602788816	20.89661392945077
15	14.248474358661822	26.367307801695066	38.50450582222114	20.87971201742197
16	16.73955616229086	24.405710896045115	34.864744074173394	23.989988867490634
17	16.35666284748462	24.85166134418956	28.967926996741504	29.82374881158431
18	18.625582018965893	23.751086841698967	33.90149760691678	23.721833532418355
19	20.776350324630474	25.16174642256405	31.412528542291344	22.649374710514127
20	23.81869448981416	26.349430779356915	29.205528875454036	20.62634585537489
21	22.056345124042156	27.377359563800656	29.999756222422665	20.566539089734526
22	20.16804400997863	25.492471335819864	28.840675101370845	25.498809552830664
23	19.11021184271471	27.388735850743117	26.976589226656266	26.524463079885912
24	21.236927427415225	23.787003404760164	31.00054443658939	23.97552473123522
25	17.784386858763398	26.525275671810373	28.947937235399756	26.742400234026476
26	18.80613994458123	29.61296246637901	27.77114161039468	23.809755978645082
27	19.622794828664993	29.261597718241877	27.932197329822937	23.183410123270196
28	16.62806855025475	28.004843047869795	32.07690369973103	23.29018470214443
29	16.736955868132583	28.128357020387934	30.222893964879777	24.91179314659971
30	19.84235716665448	30.081340451638596	26.903130916684947	23.17317146502198
31	24.481444463404923	31.403915067892058	22.841308923071924	21.2733315456311
32	22.77305120141716	33.28214004209226	22.83318300382731	21.111625752663272
33	23.706069249083804	29.66838123562728	23.395009060399957	23.23054045488896
34	20.267667779917602	29.456619780112625	26.2337176893136	24.04199475065617
35	20.888488010206157	28.10690459358215	24.72115908112105	26.283448315090645
36	25.464030618463713	26.84592444520286	25.21992800435549	22.47011693197793
37	20.55532532117696	30.626264596182445	27.038996286454903	21.779413796185693
38	17.82371630790733	35.19839431835726	24.27910907421402	22.698780299521385
39	21.724320063707207	29.973590762455004	27.721573503002528	20.580515670835265
40	24.75788823610671	26.79749396650496	26.768728212379024	21.675889585009305
41	21.943232328157123	26.471482086411026	24.696456286617423	26.888829298814425
42	24.679554374588626	25.214402379269156	24.361505895354412	25.74453735078781
43	24.555552846915806	24.38328335892998	25.3379163517873	25.72324744236692
44	20.114412942964172	27.128218879760773	26.226729398763236	26.53063877851182
45	21.057344612109244	27.634138611930474	24.28772254861331	27.02079422734697
46	23.710619763860787	27.47698333373963	26.56606778641834	22.246329115981244
47	19.993824301374094	28.06318714804612	28.699771661669228	23.24321688891056
48	20.569139383892804	26.5774440733608	28.48508487522651	24.36833166751989
49	22.669201953470985	23.5923063796592	30.082478080332837	23.65601358653698
50	22.77305120141716	24.28203440514208	27.222642061383194	25.722272332057567
51	20.53679822529924	25.264945596970655	23.871350446519262	30.326905731210847
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	77.0
1	185.0
2	293.0
3	489.0
4	685.0
5	533.5
6	382.0
7	446.0
8	510.0
9	677.0
10	844.0
11	1063.0
12	1282.0
13	1266.0
14	1250.0
15	1271.5
16	1293.0
17	1212.5
18	1132.0
19	996.0
20	860.0
21	1102.5
22	1345.0
23	1697.0
24	2049.0
25	2866.5
26	4084.5
27	4485.0
28	5655.0
29	6825.0
30	7584.5
31	8344.0
32	9514.5
33	10685.0
34	12009.5
35	13334.0
36	15395.0
37	17456.0
38	20175.5
39	22895.0
40	28743.5
41	34592.0
42	40289.5
43	45987.0
44	52243.5
45	58500.0
46	67595.5
47	76691.0
48	83741.0
49	90791.0
50	84423.0
51	78055.0
52	61868.5
53	45682.0
54	36469.0
55	27256.0
56	23034.0
57	18812.0
58	15708.0
59	12604.0
60	10905.5
61	9207.0
62	7958.0
63	6709.0
64	5684.5
65	4660.0
66	3633.0
67	2606.0
68	2058.5
69	1511.0
70	1106.5
71	702.0
72	509.5
73	317.0
74	355.5
75	263.0
76	132.0
77	143.5
78	155.0
79	166.5
80	178.0
81	101.0
82	24.0
83	32.0
84	40.0
85	20.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	615315.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.729057499858794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.93343118764643	17.088803583778667
2	13.534632977355127	7.235359661824203
3	6.342254256517288	5.085674361035243
4	3.657883076123157	3.9108706827782487
5	2.4374429192549103	3.2575275970694086
6	1.6417707375753359	2.6329790667742046
7	1.2166926863312741	2.27647341408244
8	0.8865723607364078	1.89577948863272
9	0.6695394468520169	1.6106542535998092
>10	4.544624862730157	25.463576565949314
>50	0.9598560991268237	17.322584104137157
>100	0.1624277562379362	7.499725088761291
>500	0.00980695886719615	1.7320290459921874
>1k	0.003064674645998797	2.9879630855850845
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGC	4776	0.7761878062455816	No Hit
CTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGCT	4364	0.7092302316699576	No Hit
TCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGC	4323	0.7025669778893737	No Hit
GCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGC	3204	0.5207089051949001	No Hit
TCCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTG	1571	0.2553163826657891	No Hit
TTCCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCT	948	0.15406742887789182	No Hit
TGCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTG	834	0.13554033300017065	No Hit
GCCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTG	810	0.13163989176275567	No Hit
TGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGCTT	737	0.11977604966561843	Illumina Single End Adapter 2 (95% over 21bp)
TTTCCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTC	704	0.11441294296417283	No Hit
TTCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTG	666	0.10823724433826577	No Hit
CGCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTG	666	0.10823724433826577	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCC	664	0.10791220756848117	No Hit
TTTTTTGGGAGTTACATCATCGGAGAGCGGTTTTTGAGCTTTCAATAAGCT	637	0.10352421117638932	No Hit
TTTTTCGGGATTTGGGGAAAGGGAAAATTGGCTTTCTGTTTAATTGGCAAA	623	0.10124895378789726	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.250367697845819E-4	0.0	0.0	0.7656241112275827	0.0
2	3.250367697845819E-4	0.0	0.0	2.983837546622462	0.0
3	3.250367697845819E-4	0.0	0.0	4.10310166337567	0.0
4	3.250367697845819E-4	0.0	0.0	5.270958777211672	0.0
5	3.250367697845819E-4	0.0	0.0	7.165273071516215	0.0
6	3.250367697845819E-4	0.0	0.0	8.383998439823506	0.0
7	3.250367697845819E-4	0.0	0.0	9.47043384282847	0.0
8	3.250367697845819E-4	0.0	0.0	11.320055581287633	0.0
9	3.250367697845819E-4	0.0	0.0	12.042449802133866	0.0
10	3.250367697845819E-4	0.0	0.0	13.710375986283449	0.0
11	3.250367697845819E-4	0.0	0.0	16.68982553651382	0.0
12	3.250367697845819E-4	0.0	0.0	19.268179712830015	0.0
13	3.250367697845819E-4	0.0	0.0	20.089547630075653	0.0
14	3.250367697845819E-4	0.0	0.0	20.328124619097537	0.0
15	3.250367697845819E-4	0.0	0.0	20.71719363252968	0.0
16	3.250367697845819E-4	0.0	0.0	21.812242509933935	0.0
17	3.250367697845819E-4	0.0	0.0	23.40573527380285	0.0
18	3.250367697845819E-4	0.0	0.0	25.42242591193129	0.0
19	3.250367697845819E-4	0.0	0.0	26.55404142593631	0.0
20	3.250367697845819E-4	0.0	0.0	27.717022988225544	0.0
21	3.250367697845819E-4	0.0	0.0	29.425416250213306	0.0
22	3.250367697845819E-4	0.0	0.0	30.99079333349585	0.0
23	3.250367697845819E-4	0.0	0.0	32.54365650114169	0.0
24	3.250367697845819E-4	0.0	0.0	33.676084607071175	0.0
25	3.250367697845819E-4	0.0	0.0	34.615928426903295	0.0
26	4.875551546768728E-4	0.0	0.0	35.51823049982529	0.0
27	4.875551546768728E-4	0.0	0.0	36.33797323322201	0.0
28	4.875551546768728E-4	0.0	0.0	37.168604698406504	0.0
29	4.875551546768728E-4	0.0	0.0	38.13965204813795	0.0
30	4.875551546768728E-4	0.0	0.0	39.25387809495949	0.0
31	4.875551546768728E-4	0.0	0.0	40.254178753971544	0.0
32	4.875551546768728E-4	0.0	0.0	41.102687241494195	0.0
33	4.875551546768728E-4	0.0	0.0	41.866198613718176	0.0
34	4.875551546768728E-4	0.0	0.0	42.676352762406246	0.0
35	4.875551546768728E-4	0.0	0.0	43.546801231889354	0.0
36	4.875551546768728E-4	0.0	0.0	44.36459374466737	0.0
37	4.875551546768728E-4	0.0	0.0	45.16125886740937	0.0
38	4.875551546768728E-4	0.0	0.0	45.94784785028806	0.0
39	4.875551546768728E-4	0.0	0.0	46.74370038110561	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTAAT	35	1.2107012E-7	45.000004	15
TCGGCGT	65	0.0	45.000004	4
CTTTGAC	70	0.0	45.000004	35
GCGTCCC	70	0.0	45.000004	7
TCGCGGT	35	1.2107012E-7	45.000004	13
GTCGGCG	65	0.0	45.000004	3
AGTCGCG	35	1.2107012E-7	45.000004	11
AAACTCC	20	7.0307293E-4	45.0	31
AATCCGA	25	3.888686E-5	45.0	44
GTACCGC	25	3.888686E-5	45.0	26
AGATCGA	50	2.1827873E-11	45.0	32
TGCCCCG	20	7.0307293E-4	45.0	1
ACTGCGG	25	3.888686E-5	45.0	2
TCCGCTA	20	7.0307293E-4	45.0	41
TCCGCGT	25	3.888686E-5	45.0	38
GCGACAG	20	7.0307293E-4	45.0	38
TCCGCAC	25	3.888686E-5	45.0	17
ATAGGTG	20	7.0307293E-4	45.0	36
GTCGAGG	20	7.0307293E-4	45.0	1
GTTTCGC	25	3.888686E-5	45.0	37
>>END_MODULE