##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935298.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1146429
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23785336902678	33.0	31.0	34.0	30.0	34.0
2	32.38432820523556	34.0	31.0	34.0	30.0	34.0
3	32.43586301463065	34.0	31.0	34.0	30.0	34.0
4	35.92503068223152	37.0	35.0	37.0	35.0	37.0
5	31.677881491134645	37.0	35.0	37.0	0.0	37.0
6	33.417700529208524	37.0	35.0	37.0	17.0	37.0
7	35.21338259935853	37.0	35.0	37.0	32.0	37.0
8	35.754767194479555	37.0	35.0	37.0	35.0	37.0
9	37.794824624987676	39.0	38.0	39.0	35.0	39.0
10	37.46882100854043	39.0	37.0	39.0	35.0	39.0
11	37.36595637409731	39.0	37.0	39.0	35.0	39.0
12	37.112348867657744	39.0	37.0	39.0	34.0	39.0
13	36.983999881370764	39.0	37.0	39.0	33.0	39.0
14	38.11047347895072	40.0	37.0	41.0	33.0	41.0
15	38.21864502729781	40.0	37.0	41.0	34.0	41.0
16	38.322579941714665	40.0	37.0	41.0	34.0	41.0
17	38.326190283044134	40.0	37.0	41.0	34.0	41.0
18	38.31464224997797	40.0	37.0	41.0	34.0	41.0
19	38.339338938564886	40.0	37.0	41.0	34.0	41.0
20	38.267100710118115	40.0	37.0	41.0	34.0	41.0
21	38.19988852340616	40.0	37.0	41.0	34.0	41.0
22	38.17454722446833	40.0	37.0	41.0	34.0	41.0
23	38.140281692106534	40.0	37.0	41.0	34.0	41.0
24	38.09293554158173	40.0	37.0	41.0	34.0	41.0
25	38.02611151671844	40.0	36.0	41.0	34.0	41.0
26	38.00273196159553	40.0	36.0	41.0	34.0	41.0
27	37.93994481995832	40.0	36.0	41.0	34.0	41.0
28	37.92264763016288	40.0	36.0	41.0	34.0	41.0
29	37.887971256833175	40.0	36.0	41.0	34.0	41.0
30	37.7938633792411	40.0	36.0	41.0	33.0	41.0
31	37.59666494828725	40.0	36.0	41.0	33.0	41.0
32	37.5948488741998	39.0	36.0	41.0	33.0	41.0
33	37.58548763159341	39.0	36.0	41.0	33.0	41.0
34	37.58044414438225	39.0	36.0	41.0	33.0	41.0
35	37.502958316651096	39.0	35.0	41.0	33.0	41.0
36	37.486600565756795	39.0	35.0	41.0	33.0	41.0
37	37.47528194070457	39.0	35.0	41.0	33.0	41.0
38	37.46550462348737	39.0	35.0	41.0	33.0	41.0
39	37.42239685144043	39.0	35.0	41.0	33.0	41.0
40	37.34605283013602	39.0	35.0	41.0	33.0	41.0
41	37.291010607721894	39.0	35.0	41.0	33.0	41.0
42	37.300955401512	39.0	35.0	41.0	33.0	41.0
43	37.2936911051622	39.0	35.0	41.0	33.0	41.0
44	37.26594233048885	39.0	35.0	41.0	33.0	41.0
45	37.25905049505901	39.0	35.0	41.0	33.0	41.0
46	37.11864930143951	39.0	35.0	41.0	32.0	41.0
47	37.059481223869945	39.0	35.0	41.0	32.0	41.0
48	37.051806958826056	39.0	35.0	41.0	32.0	41.0
49	37.05042789392104	39.0	35.0	41.0	32.0	41.0
50	36.99526180862487	39.0	35.0	41.0	32.0	41.0
51	36.3084831245546	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	2.0
11	3.0
12	12.0
13	10.0
14	10.0
15	23.0
16	33.0
17	60.0
18	128.0
19	189.0
20	358.0
21	623.0
22	1023.0
23	1590.0
24	2324.0
25	3388.0
26	4852.0
27	6325.0
28	8269.0
29	10765.0
30	14825.0
31	20340.0
32	28171.0
33	43398.0
34	73747.0
35	101416.0
36	96112.0
37	151561.0
38	248065.0
39	328533.0
40	271.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.885797550480667	4.502764671863674	54.43224133374156	16.1791964439141
2	20.16287096715104	5.063549508953455	53.01593033672386	21.757649187171644
3	20.53105774539897	4.8917987943431305	52.0751830248537	22.501960435404197
4	18.970123749486447	4.662041870887774	51.412342151149346	24.955492228476427
5	16.000816448292916	17.202286404129694	45.53199543975248	21.264901707824908
6	21.14199832697882	6.676994388662534	53.288428677222925	18.892578607135725
7	80.9921940216097	2.5552389201599053	10.757404078229005	5.695162980001378
8	81.19761450556467	3.20621686995008	8.744283335470405	6.851885289014844
9	76.29011478251161	5.048023035006965	11.507297878891759	7.154564303589668
10	37.98011041241978	24.380663782929428	21.20227244774862	16.436953356902173
11	29.54975842376632	25.32786592104701	24.664240000907164	20.458135654279506
12	25.998382804342878	20.77450936778466	32.34434927937099	20.882758548501474
13	22.498820249662213	26.15443259024327	32.12418736790503	19.222559792189486
14	19.256927380587896	28.705397368698804	30.44820045550139	21.589474795211917
15	17.038473381256054	25.820700627775466	36.7219426584638	20.418883332504674
16	18.229999415576543	23.764140648919383	33.779850300367485	24.226009635136585
17	17.887108578027945	24.888239917168878	29.388387767580898	27.83626373722228
18	19.17981837514578	23.2983464305247	33.670467163688286	23.851368030641236
19	21.856041673753893	26.15172854140989	29.532400174803676	22.459829610032543
20	24.077199721919108	26.070258166881683	30.13392019915756	19.71862191204165
21	21.922160029099054	27.73342265417222	29.10960905559786	21.23480826113087
22	20.216864716436866	25.50136118329177	27.997808848171147	26.283965252100217
23	19.926484762684822	26.481709726463652	27.29493060625647	26.296874904595054
24	21.252602647002124	24.199230828947975	30.18965849607782	24.358508027972075
25	18.01498391963218	26.733011813204303	28.059391379666778	27.192612887496743
26	17.442248931246507	26.850681551147083	30.73116608180707	24.97590343579934
27	19.271232671190276	26.037024534445656	29.602356534944597	25.089386259419467
28	16.485539008521243	24.13014674262427	32.79357029523852	26.59074395361597
29	19.754908502837942	23.088128440575037	30.977234525644416	26.179728530942604
30	20.594210369765594	26.029522979617575	28.30764050804716	25.068626142569666
31	23.33838379873503	27.24207081293303	24.721373935934977	24.698171452396963
32	21.639543312320257	29.42345317503308	25.151928292114036	23.78507522053263
33	21.314446860642917	29.013397253558658	24.150994086855793	25.521161798942632
34	19.907033056560852	26.035279986811222	27.998419439843197	26.05926751678473
35	19.02019226659479	25.996376574563275	28.248849252766632	26.7345819060753
36	20.33148149601938	27.437809057516866	27.27556612751422	24.95514331894954
37	19.828353958247742	26.499591339716634	28.249983208729017	25.422071493306607
38	20.516490772651423	29.17415731807203	24.72617144192968	25.583180467346867
39	22.93879516306723	27.558008389529576	27.519977251098847	21.98321919630435
40	23.206583224953313	26.289983941439026	27.855366533819364	22.6480662997883
41	18.777264008499436	27.295715652691964	26.667416822149477	27.25960351665912
42	19.545301104560334	26.63645110163822	27.070407325704426	26.747840468097024
43	21.189362795253782	24.435180896505585	27.451067619538584	26.92438868870205
44	20.210584344952892	23.52034011700681	27.7393541161293	28.529721421911
45	22.14476430725322	23.329224923654234	26.143005803237706	28.38300496585484
46	22.534496248786446	24.856838059749013	28.21858135130915	24.390084340155386
47	18.43864731265521	25.264626069298668	30.963888736240968	25.332837881805155
48	19.121550484155584	24.443729179914325	29.46619459207679	26.968525743853306
49	20.86976166862492	22.5255118284691	31.29779515347222	25.306931349433764
50	21.46910100843576	22.23242782588368	28.71883038548397	27.57964078019659
51	19.338659437261267	22.9007640246365	25.830295639764874	31.930280898337358
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	343.0
1	689.5
2	1036.0
3	1489.5
4	1943.0
5	1482.0
6	1021.0
7	1089.0
8	1157.0
9	1359.0
10	1561.0
11	1681.0
12	1801.0
13	1864.0
14	1927.0
15	1994.5
16	2062.0
17	1904.0
18	1746.0
19	2195.5
20	2645.0
21	2833.5
22	3022.0
23	3620.5
24	4219.0
25	5073.5
26	8039.5
27	10151.0
28	11483.0
29	12815.0
30	14081.0
31	15347.0
32	16819.5
33	18292.0
34	20875.5
35	23459.0
36	26692.0
37	29925.0
38	33557.5
39	37190.0
40	44627.0
41	52064.0
42	63171.0
43	74278.0
44	84907.0
45	95536.0
46	113613.5
47	131691.0
48	147247.5
49	162804.0
50	153278.5
51	143753.0
52	118445.0
53	93137.0
54	77040.5
55	60944.0
56	51912.0
57	42880.0
58	37915.5
59	32951.0
60	29700.5
61	26450.0
62	22387.5
63	18325.0
64	15177.5
65	12030.0
66	10090.5
67	8151.0
68	6654.0
69	5157.0
70	4377.5
71	3598.0
72	2968.0
73	2338.0
74	1882.0
75	1060.5
76	695.0
77	538.0
78	381.0
79	289.5
80	198.0
81	120.0
82	42.0
83	24.5
84	7.0
85	4.5
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1146429.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.152330822788038
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.24965077900573	16.541257915983714
2	13.45454319180264	7.0373532924302635
3	6.374699281812347	5.001397335412375
4	3.837287139066613	4.014160108915997
5	2.461741508169156	3.219013916091447
6	1.6633902470560502	2.6100919217045373
7	1.2591142850095942	2.3050141327688394
8	0.9724688325211317	2.0345861298354473
9	0.7798055533883715	1.8354359528693995
>10	4.874988771852352	27.086936001982536
>50	0.9123639720244872	16.12722815422714
>100	0.1456179031951777	6.532582492973943
>500	0.008996987153523933	1.677807565616475
>1k	0.0039986609571217475	1.4900095443261046
>5k	0.0013328869857072493	2.487125534861798
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	8417	0.7341928719528205	No Hit
CTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGCT	7564	0.6597879153440814	No Hit
TCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	6404	0.5586041525467342	No Hit
GCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	6155	0.5368845344979933	No Hit
TCCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTG	2550	0.2224298233907202	No Hit
GCCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTG	1742	0.15195009895946457	No Hit
TTCCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCT	1654	0.14427408936794167	No Hit
TGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTG	1573	0.13720867144847174	No Hit
TGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGCTT	1522	0.13276007498065734	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCC	1361	0.11871646652343931	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGC	1193	0.10406226639416832	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCG	1149	0.10022426159840689	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6793268488497761	0.0
2	0.0	0.0	0.0	2.6354881113440083	0.0
3	0.0	0.0	0.0	3.6714004966727116	0.0
4	0.0	0.0	0.0	4.863973259573859	0.0
5	0.0	0.0	0.0	6.798676586164516	0.0
6	0.0	0.0	0.0	7.982788293038644	0.0
7	0.0	0.0	0.0	9.10086887194933	0.0
8	0.0	0.0	0.0	10.876120544752444	0.0
9	0.0	0.0	0.0	11.622001885855992	0.0
10	0.0	0.0	0.0	13.351371955873413	0.0
11	0.0	0.0	0.0	16.34309669416946	0.0
12	0.0	0.0	0.0	19.052291943068433	0.0
13	8.722738172185107E-5	0.0	0.0	19.85993027043105	0.0
14	8.722738172185107E-5	0.0	0.0	20.122135779886936	0.0
15	8.722738172185107E-5	0.0	0.0	20.57379916244268	0.0
16	8.722738172185107E-5	0.0	0.0	21.70374266526754	0.0
17	8.722738172185107E-5	0.0	0.0	23.32233396049821	0.0
18	8.722738172185107E-5	0.0	0.0	25.225809884432444	0.0
19	8.722738172185107E-5	0.0	0.0	26.394133435214915	0.0
20	8.722738172185107E-5	0.0	0.0	27.613746686449836	0.0
21	8.722738172185107E-5	0.0	0.0	29.192562295615343	0.0
22	8.722738172185107E-5	0.0	0.0	30.713982287607866	0.0
23	8.722738172185107E-5	0.0	0.0	32.252847755944764	0.0
24	8.722738172185107E-5	0.0	0.0	33.4270155413026	0.0
25	8.722738172185107E-5	0.0	0.0	34.44722699792137	0.0
26	8.722738172185107E-5	0.0	0.0	35.36991824177511	0.0
27	8.722738172185107E-5	0.0	0.0	36.21026683728343	0.0
28	8.722738172185107E-5	0.0	0.0	37.10766214043783	0.0
29	8.722738172185107E-5	0.0	0.0	38.07326925609872	0.0
30	8.722738172185107E-5	0.0	0.0	39.09967385681974	0.0
31	8.722738172185107E-5	0.0	0.0	40.078452307120635	0.0
32	8.722738172185107E-5	0.0	0.0	41.031324225050135	0.0
33	8.722738172185107E-5	0.0	0.0	41.877080918225204	0.0
34	8.722738172185107E-5	0.0	0.0	42.73897467701881	0.0
35	8.722738172185107E-5	0.0	0.0	43.65599614106063	0.0
36	8.722738172185107E-5	0.0	0.0	44.51719208080047	0.0
37	8.722738172185107E-5	0.0	0.0	45.39400172186851	0.0
38	8.722738172185107E-5	0.0	0.0	46.26993908911934	0.0
39	8.722738172185107E-5	0.0	0.0	47.08882974872408	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAGTCG	20	7.0332806E-4	45.0	15
CGAACGA	25	3.8908E-5	45.0	22
CACGGTA	20	7.0332806E-4	45.0	44
GTACACG	20	7.0332806E-4	45.0	36
CGAAACA	25	3.8908E-5	45.0	44
TCCGATA	25	3.8908E-5	45.0	14
ACGTTAG	20	7.0332806E-4	45.0	13
GTTTACG	80	0.0	45.0	1
CGGCACG	20	7.0332806E-4	45.0	18
TAAACCG	20	7.0332806E-4	45.0	15
CAACCCG	20	7.0332806E-4	45.0	23
ACCTCGT	20	7.0332806E-4	45.0	23
CGCCTCA	20	7.0332806E-4	45.0	18
ATTCCCG	25	3.8908E-5	45.0	1
TCGTGTT	20	7.0332806E-4	45.0	12
ACGGCCC	20	7.0332806E-4	45.0	14
TACGACA	25	3.8908E-5	45.0	23
CGCTAAA	25	3.8908E-5	45.0	19
GTCACGA	25	3.8908E-5	45.0	16
TATTACG	20	7.0332806E-4	45.0	1
>>END_MODULE