SRR2935297.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935297.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	986526
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	5844	0.5923817517227118	TruSeq Adapter, Index 13 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	5813	0.5892394118350657	TruSeq Adapter, Index 13 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGCT	5738	0.5816369766230185	Illumina PCR Primer Index 4 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	4187	0.4244186164378841	TruSeq Adapter, Index 13 (96% over 25bp)
TCCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTG	2201	0.2231061320228762	Illumina PCR Primer Index 4 (95% over 21bp)
TTCCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCT	1353	0.1371479312253301	TruSeq Adapter, Index 13 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTG	1148	0.11636794164573462	RNA PCR Primer, Index 13 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTG	1108	0.11231330953264283	TruSeq Adapter, Index 13 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCC	994	0.1007576080103312	No Hit
TTCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTG	993	0.10065624220750391	RNA PCR Primer, Index 13 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTCGCG	290	0.0	45.000004	1
GTCCCGC	65	0.0	45.000004	10
AACGTAA	20	7.032801E-4	45.0	37
ATGGGCG	170	0.0	45.0	5
GGTACGT	95	0.0	45.0	8
TCGTTAG	35	1.2117016E-7	45.0	1
TCGCACG	20	7.032801E-4	45.0	1
GCGACTT	20	7.032801E-4	45.0	31
ACTGCCG	25	3.8904032E-5	45.0	10
CATGCGT	25	3.8904032E-5	45.0	13
GCGCGAC	160	0.0	45.0	9
CGGTTAC	25	3.8904032E-5	45.0	44
GACCGGA	35	1.2117016E-7	45.0	11
GCGTTTC	20	7.032801E-4	45.0	28
AAGCGCG	20	7.032801E-4	45.0	12
CGCGACA	20	7.032801E-4	45.0	37
CGACACT	20	7.032801E-4	45.0	37
ATCGTAA	20	7.032801E-4	45.0	20
TATTGCG	45	3.8380676E-10	45.0	1
GAGACGA	20	7.032801E-4	45.0	9