##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935297.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	986526
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29013224182637	33.0	31.0	34.0	30.0	34.0
2	32.434589661093575	34.0	31.0	34.0	30.0	34.0
3	32.49355212128216	34.0	31.0	34.0	30.0	34.0
4	35.96196248248906	37.0	35.0	37.0	35.0	37.0
5	31.72379136484999	37.0	35.0	37.0	0.0	37.0
6	33.48982490071219	37.0	35.0	37.0	17.0	37.0
7	35.30252218390595	37.0	35.0	37.0	32.0	37.0
8	35.842553566758504	37.0	35.0	37.0	35.0	37.0
9	37.88619864048185	39.0	38.0	39.0	35.0	39.0
10	37.49514761901866	39.0	37.0	39.0	35.0	39.0
11	37.39375242010854	39.0	37.0	39.0	35.0	39.0
12	37.18906952274953	39.0	37.0	39.0	34.0	39.0
13	37.11565229907777	39.0	37.0	39.0	33.0	39.0
14	38.2624228859655	40.0	38.0	41.0	34.0	41.0
15	38.372093589018434	40.0	38.0	41.0	34.0	41.0
16	38.45540411504613	40.0	38.0	41.0	34.0	41.0
17	38.46777783859726	40.0	38.0	41.0	34.0	41.0
18	38.45151369553362	40.0	37.0	41.0	34.0	41.0
19	38.49681103184305	40.0	38.0	41.0	34.0	41.0
20	38.429622736755036	40.0	37.0	41.0	34.0	41.0
21	38.35580410450409	40.0	37.0	41.0	34.0	41.0
22	38.3316182239495	40.0	37.0	41.0	34.0	41.0
23	38.29441393333779	40.0	37.0	41.0	34.0	41.0
24	38.27578087146208	40.0	37.0	41.0	34.0	41.0
25	38.208141498551484	40.0	37.0	41.0	34.0	41.0
26	38.16820337223753	40.0	37.0	41.0	34.0	41.0
27	38.133123708853084	40.0	37.0	41.0	34.0	41.0
28	38.126425456602256	40.0	37.0	41.0	34.0	41.0
29	38.06910208144539	40.0	37.0	41.0	34.0	41.0
30	38.022188974238894	40.0	37.0	41.0	34.0	41.0
31	37.83126141632354	40.0	36.0	41.0	34.0	41.0
32	37.83726835379909	40.0	36.0	41.0	34.0	41.0
33	37.86135388220888	40.0	36.0	41.0	34.0	41.0
34	37.87158371903021	40.0	36.0	41.0	34.0	41.0
35	37.801209496759334	40.0	36.0	41.0	33.0	41.0
36	37.816982015679265	40.0	36.0	41.0	33.0	41.0
37	37.78752105874554	40.0	36.0	41.0	33.0	41.0
38	37.751429764648876	40.0	36.0	41.0	33.0	41.0
39	37.74103064693683	40.0	36.0	41.0	33.0	41.0
40	37.68650699525406	40.0	36.0	41.0	33.0	41.0
41	37.65802219100156	40.0	36.0	41.0	33.0	41.0
42	37.668806498764354	40.0	36.0	41.0	33.0	41.0
43	37.648700591773554	40.0	36.0	41.0	33.0	41.0
44	37.60160097148985	40.0	36.0	41.0	33.0	41.0
45	37.582353632849006	40.0	36.0	41.0	33.0	41.0
46	37.46640534562698	39.0	35.0	41.0	33.0	41.0
47	37.42839316956674	39.0	35.0	41.0	33.0	41.0
48	37.42194833182298	39.0	35.0	41.0	33.0	41.0
49	37.43304585991652	39.0	35.0	41.0	33.0	41.0
50	37.371242116274686	39.0	35.0	41.0	33.0	41.0
51	36.73868909689152	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	5.0
11	6.0
12	4.0
13	5.0
14	9.0
15	16.0
16	21.0
17	35.0
18	62.0
19	117.0
20	235.0
21	384.0
22	593.0
23	1054.0
24	1679.0
25	2569.0
26	3638.0
27	4904.0
28	6298.0
29	8383.0
30	11362.0
31	15725.0
32	21817.0
33	33301.0
34	55950.0
35	83481.0
36	77612.0
37	125144.0
38	215735.0
39	316029.0
40	347.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.758345953375787	4.053415723457872	57.44197314617151	15.746265176994829
2	18.95459420228154	3.9300535414170534	55.44962829160103	21.665723964700373
3	19.682096569173037	4.038312218836604	54.22148022454552	22.05811098744483
4	19.240952595268652	4.617921879403077	52.449707356927235	23.691418168401036
5	14.865294984622807	16.726979319348906	46.15935109667662	22.248374599351664
6	19.957608821257626	5.822451714399824	55.470712378589106	18.749227085753443
7	83.64432361640748	1.5542418547509138	9.631069024029777	5.170365504811834
8	85.65015012275398	1.612324459770954	6.9963690769427265	5.741156340532333
9	80.6337592724368	4.5869039437379255	9.505476794326759	5.2738599894985025
10	35.916336720978464	32.7211852500593	17.69552956536371	13.666948463598526
11	26.77638501164693	25.231874273967435	28.079442406991806	19.912298307393826
12	23.777275003395754	22.261754885324866	33.55218210163746	20.408788009641913
13	21.63014456790799	27.401710649288514	32.99010872496011	17.978036057843383
14	18.88049580041479	29.547421963536692	32.12687754808287	19.44520468796565
15	16.709037572248477	27.178705883068467	36.23057070974308	19.88168583493998
16	17.387782987980042	25.672815516266173	33.91760582082986	23.021795674923926
17	18.06581884309182	24.53103111321952	30.384703494890147	27.018446548798515
18	18.343662508641433	24.263019930544154	33.88891929862974	23.504398262184676
19	20.504781424919365	25.609461889499112	31.734794622746893	22.150962062834633
20	23.504803725395988	26.842475515090324	29.80458700530954	19.84813375420415
21	22.051319478655405	27.127110689429372	31.378595191611776	19.442974640303447
22	20.791748012723435	25.621929883246867	28.853978506395165	24.732343597634525
23	18.732907191498246	27.123258788921934	27.995714253856463	26.148119765723354
24	19.68270476399	25.672207321449207	30.895283043731236	23.749804870829557
25	17.73080486474761	27.154986285206878	28.690374100631917	26.4238347494136
26	17.288039037997986	28.13225399026483	29.053263674753634	25.526443296983558
27	18.375288639123553	27.363698473228276	31.09558187011797	23.165431017530203
28	15.213081054123256	25.44129602260863	33.63398430451909	25.71163861874902
29	15.716463630963604	25.675349661336856	31.727800382351806	26.880386325347732
30	18.516288470856317	27.51686220130032	30.97779480723265	22.989054520610708
31	21.143487348534148	29.15067621127066	26.399202859326564	23.306633580868624
32	18.947295864477976	29.053466406359284	27.21235932960713	24.78687839955561
33	19.422397382329507	27.294566995700066	27.260508085950093	26.02252753602034
34	18.311326817539527	25.505257844192652	29.00764906348135	27.175766274786472
35	16.66433525320164	25.29320058467795	28.501326878359006	29.541137283761397
36	19.56430950628772	25.205823262640827	29.96089307326923	25.268974157802226
37	17.9799620080971	25.89095472395051	31.281182655094746	24.847900612857643
38	16.633317317536488	29.69075320873449	28.484398789286853	25.191530684442178
39	18.445535140482868	29.276369806776508	28.363874849725196	23.91422020301543
40	22.14214323798866	25.54022904616807	29.533940311760666	22.78368740408261
41	19.450070246501358	23.1973612454208	29.813101732747032	27.539466775330805
42	19.85776350547274	22.496416718870055	28.80187648374194	28.843943291915263
43	19.84793102259849	22.947697273057173	29.341548017994455	27.86282368634988
44	17.978340155251864	25.418184619564006	28.166414265817625	28.437060959366505
45	19.42939162272459	25.307999991890735	26.752259950574036	28.510348434810638
46	21.301111171930593	25.600034869836175	29.17094937183612	23.927904586397116
47	17.522295408331864	25.00663946008519	31.72354301863306	25.747522112949888
48	17.36842211964003	24.068803052327052	31.186506995254053	27.37626783277886
49	19.44550878537413	22.486786967601464	31.84193827633534	26.225765970689064
50	19.896383876349937	23.08200696180334	29.892471156360806	27.12913800548592
51	18.976894678903548	22.431542605060585	27.66759315010451	30.92396956593136
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	122.0
1	277.5
2	433.0
3	794.5
4	1156.0
5	903.0
6	650.0
7	729.0
8	808.0
9	1016.0
10	1224.0
11	1515.0
12	1806.0
13	1594.0
14	1382.0
15	1491.0
16	1600.0
17	1557.5
18	1515.0
19	1716.0
20	1917.0
21	2259.0
22	2601.0
23	2875.5
24	3150.0
25	4439.0
26	7460.0
27	9192.0
28	11046.0
29	12900.0
30	14289.0
31	15678.0
32	18553.5
33	21429.0
34	24635.5
35	27842.0
36	31002.5
37	34163.0
38	38933.0
39	43703.0
40	49951.0
41	56199.0
42	66637.5
43	77076.0
44	83988.0
45	90900.0
46	103216.0
47	115532.0
48	127935.0
49	140338.0
50	128236.0
51	116134.0
52	92278.5
53	68423.0
54	56573.5
55	44724.0
56	36965.5
57	29207.0
58	24749.5
59	20292.0
60	16747.0
61	13202.0
62	11269.5
63	9337.0
64	8168.5
65	7000.0
66	5259.5
67	3519.0
68	3001.0
69	2483.0
70	1880.0
71	1277.0
72	1032.5
73	788.0
74	700.5
75	472.5
76	332.0
77	223.5
78	115.0
79	67.5
80	20.0
81	17.5
82	15.0
83	7.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	986526.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.131411878330457
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.20939039216378	16.778820267941462
2	13.354012505882013	6.979184020391574
3	6.27431911268182	4.918704509687085
4	3.6285504172727827	3.7927658190017173
5	2.31879296945663	3.029666707272406
6	1.5885878118467738	2.4907225449758266
7	1.187056342902606	2.1713620753420866
8	0.8902651158586782	1.8611107538730163
9	0.6734921595878346	1.5839370917114373
>10	4.659946125342903	26.31709925027337
>50	1.0509803844469066	18.626512964461586
>100	0.15053103345499336	6.7446226858376574
>500	0.010947711523999152	1.9068197655883794
>1k	0.00195494848642842	1.0214047686109966
>5k	0.001172969091857052	1.777266775031338
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	5844	0.5923817517227118	TruSeq Adapter, Index 13 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	5813	0.5892394118350657	TruSeq Adapter, Index 13 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGCT	5738	0.5816369766230185	Illumina PCR Primer Index 4 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	4187	0.4244186164378841	TruSeq Adapter, Index 13 (96% over 25bp)
TCCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTG	2201	0.2231061320228762	Illumina PCR Primer Index 4 (95% over 21bp)
TTCCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCT	1353	0.1371479312253301	TruSeq Adapter, Index 13 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTG	1148	0.11636794164573462	RNA PCR Primer, Index 13 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTG	1108	0.11231330953264283	TruSeq Adapter, Index 13 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCC	994	0.1007576080103312	No Hit
TTCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTG	993	0.10065624220750391	RNA PCR Primer, Index 13 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0136580282729498E-4	0.0	0.0	0.6073838905411515	0.0
2	1.0136580282729498E-4	0.0	0.0	2.3300957095910295	0.0
3	1.0136580282729498E-4	0.0	0.0	3.2344814024161552	0.0
4	1.0136580282729498E-4	0.0	0.0	4.247733967477796	0.0
5	1.0136580282729498E-4	0.0	0.0	5.836642926795644	0.0
6	1.0136580282729498E-4	0.0	0.0	6.923284333104246	0.0
7	1.0136580282729498E-4	0.0	0.0	7.894267358386905	0.0
8	1.0136580282729498E-4	0.0	0.0	9.520478933145199	0.0
9	1.0136580282729498E-4	0.0	0.0	10.194865619355191	0.0
10	1.0136580282729498E-4	0.0	0.0	11.540496651887533	0.0
11	1.0136580282729498E-4	0.0	0.0	14.205099510808635	0.0
12	1.0136580282729498E-4	0.0	0.0	16.728094343180008	0.0
13	1.0136580282729498E-4	0.0	0.0	17.44525739818312	0.0
14	1.0136580282729498E-4	0.0	0.0	17.666437579952277	0.0
15	1.0136580282729498E-4	0.0	0.0	17.994153220492922	0.0
16	1.0136580282729498E-4	0.0	0.0	18.95459420228154	0.0
17	1.0136580282729498E-4	0.0	0.0	20.448827501758696	0.0
18	1.0136580282729498E-4	0.0	0.0	22.18745375185246	0.0
19	1.0136580282729498E-4	0.0	0.0	23.219560356239977	0.0
20	1.0136580282729498E-4	0.0	0.0	24.28704362581422	0.0
21	1.0136580282729498E-4	0.0	0.0	25.730594023877728	0.0
22	1.0136580282729498E-4	0.0	0.0	27.24226224144118	0.0
23	1.0136580282729498E-4	0.0	0.0	28.663512162882682	0.0
24	1.0136580282729498E-4	0.0	0.0	29.765054342206895	0.0
25	1.0136580282729498E-4	0.0	0.0	30.70512079762723	0.0
26	1.0136580282729498E-4	0.0	0.0	31.581428163069194	0.0
27	1.0136580282729498E-4	0.0	0.0	32.36366806348743	0.0
28	1.0136580282729498E-4	0.0	0.0	33.2247705585053	0.0
29	1.0136580282729498E-4	0.0	0.0	34.14405702434604	0.0
30	1.0136580282729498E-4	0.0	0.0	35.14879486197019	0.0
31	1.0136580282729498E-4	0.0	0.0	36.094943265560154	0.0
32	1.0136580282729498E-4	0.0	0.0	36.913066660179254	0.0
33	2.0273160565458995E-4	0.0	0.0	37.721864400938244	0.0
34	2.0273160565458995E-4	0.0	0.0	38.547286133360906	0.0
35	3.040974084818849E-4	0.0	0.0	39.47245181576562	0.0
36	3.040974084818849E-4	0.0	0.0	40.264321467452454	0.0
37	3.040974084818849E-4	0.0	0.0	41.042608101560425	0.0
38	3.040974084818849E-4	0.0	0.0	41.83305863200767	0.0
39	3.040974084818849E-4	0.0	0.0	42.594011713832174	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTCGCG	290	0.0	45.000004	1
GTCCCGC	65	0.0	45.000004	10
AACGTAA	20	7.032801E-4	45.0	37
ATGGGCG	170	0.0	45.0	5
GGTACGT	95	0.0	45.0	8
TCGTTAG	35	1.2117016E-7	45.0	1
TCGCACG	20	7.032801E-4	45.0	1
GCGACTT	20	7.032801E-4	45.0	31
ACTGCCG	25	3.8904032E-5	45.0	10
CATGCGT	25	3.8904032E-5	45.0	13
GCGCGAC	160	0.0	45.0	9
CGGTTAC	25	3.8904032E-5	45.0	44
GACCGGA	35	1.2117016E-7	45.0	11
GCGTTTC	20	7.032801E-4	45.0	28
AAGCGCG	20	7.032801E-4	45.0	12
CGCGACA	20	7.032801E-4	45.0	37
CGACACT	20	7.032801E-4	45.0	37
ATCGTAA	20	7.032801E-4	45.0	20
TATTGCG	45	3.8380676E-10	45.0	1
GAGACGA	20	7.032801E-4	45.0	9
>>END_MODULE