SRR2935296.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935296.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1866124
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	9509	0.5095588503229153	TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGCT	7034	0.37693100780012473	TruSeq Adapter, Index 14 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	5736	0.3073750726103946	TruSeq Adapter, Index 15 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	4922	0.26375524884734347	TruSeq Adapter, Index 15 (95% over 22bp)
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	2681	0.1436667659812531	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	2529	0.13552154090510599	No Hit
TCCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTG	1966	0.10535205591911363	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGATA	35	1.2124656E-7	45.000004	9
GGTACGA	45	3.8562575E-10	45.0	8
ACGTTAC	20	7.0344185E-4	45.0	9
GTTCGAC	20	7.0344185E-4	45.0	15
CGTACCG	20	7.0344185E-4	45.0	15
TTAGTCG	25	3.8917446E-5	45.0	38
TCGCGAT	25	3.8917446E-5	45.0	31
TACGGGA	940	0.0	43.324467	4
TTACGGG	1235	0.0	42.085022	3
TACGGGC	395	0.0	41.58228	4
TCTACGG	390	0.0	41.53846	2
CTACGGG	550	0.0	41.318184	3
TTTACGG	790	0.0	41.29747	2
TAACGGG	55	6.184564E-11	40.909092	3
GTACGGG	430	0.0	40.2907	3
TAGCGAT	45	1.929402E-8	40.0	11
CTATGGG	1775	0.0	39.676056	3
TGACGGG	590	0.0	39.66102	3
TACGGGG	865	0.0	39.53757	4
TGATGGG	1615	0.0	39.427242	3