##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935295.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	801984
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.398525406990665	33.0	31.0	34.0	31.0	34.0
2	32.55351852406033	34.0	31.0	34.0	31.0	34.0
3	32.60554075891788	34.0	31.0	34.0	31.0	34.0
4	36.019886930412575	37.0	35.0	37.0	35.0	37.0
5	31.628712044130555	37.0	35.0	37.0	0.0	37.0
6	33.426749162078046	37.0	35.0	37.0	17.0	37.0
7	35.232080689889074	37.0	35.0	37.0	32.0	37.0
8	35.78313407748783	37.0	35.0	37.0	35.0	37.0
9	37.865742957465486	39.0	38.0	39.0	35.0	39.0
10	37.628813043651746	39.0	37.0	39.0	35.0	39.0
11	37.50645025337164	39.0	37.0	39.0	35.0	39.0
12	37.145284693958985	39.0	37.0	39.0	34.0	39.0
13	37.01097652821004	39.0	37.0	39.0	33.0	39.0
14	38.213408247546084	40.0	37.0	41.0	33.0	41.0
15	38.316149698747104	40.0	37.0	41.0	34.0	41.0
16	38.41163290040699	40.0	37.0	41.0	34.0	41.0
17	38.42384386720932	40.0	37.0	41.0	34.0	41.0
18	38.41616166906073	40.0	37.0	41.0	35.0	41.0
19	38.421110645598915	40.0	37.0	41.0	34.0	41.0
20	38.34618271686218	40.0	37.0	41.0	34.0	41.0
21	38.27094430811587	40.0	37.0	41.0	34.0	41.0
22	38.24343752493815	40.0	36.0	41.0	34.0	41.0
23	38.20161000718219	40.0	36.0	41.0	34.0	41.0
24	38.18651993057218	40.0	36.0	41.0	34.0	41.0
25	38.08196298180513	40.0	36.0	41.0	34.0	41.0
26	37.99349114196792	40.0	36.0	41.0	34.0	41.0
27	37.94812988787806	40.0	36.0	41.0	34.0	41.0
28	37.88720971989466	40.0	36.0	41.0	34.0	41.0
29	37.83045547043333	40.0	36.0	41.0	34.0	41.0
30	37.80337762349374	40.0	36.0	41.0	34.0	41.0
31	37.56883429095842	40.0	35.0	41.0	33.0	41.0
32	37.56581428058415	39.0	35.0	41.0	33.0	41.0
33	37.59426247905195	39.0	35.0	41.0	33.0	41.0
34	37.53035472029367	39.0	35.0	41.0	33.0	41.0
35	37.44653883369244	39.0	35.0	41.0	33.0	41.0
36	37.43310589737451	39.0	35.0	41.0	33.0	41.0
37	37.36349104221531	39.0	35.0	41.0	33.0	41.0
38	37.331206108850054	39.0	35.0	41.0	33.0	41.0
39	37.37169195395419	39.0	35.0	41.0	33.0	41.0
40	37.29653459420637	39.0	35.0	41.0	33.0	41.0
41	37.24707101388557	39.0	35.0	41.0	33.0	41.0
42	37.21406162716463	39.0	35.0	41.0	33.0	41.0
43	37.13513486553348	39.0	35.0	41.0	33.0	41.0
44	37.05360705450483	39.0	35.0	41.0	33.0	41.0
45	37.06544519591413	39.0	35.0	41.0	33.0	41.0
46	36.98923544609369	39.0	35.0	41.0	33.0	41.0
47	36.95045412377304	39.0	35.0	41.0	33.0	41.0
48	36.91998468797382	39.0	35.0	41.0	33.0	41.0
49	36.878054923789	39.0	35.0	41.0	33.0	41.0
50	36.78625633429096	38.0	35.0	41.0	32.0	41.0
51	36.159274998004946	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	3.0
10	7.0
11	11.0
12	9.0
13	11.0
14	11.0
15	18.0
16	29.0
17	66.0
18	94.0
19	168.0
20	290.0
21	484.0
22	702.0
23	1098.0
24	1670.0
25	2610.0
26	3555.0
27	4607.0
28	5849.0
29	7298.0
30	9557.0
31	13006.0
32	18360.0
33	29424.0
34	52867.0
35	84293.0
36	64471.0
37	102146.0
38	160451.0
39	238428.0
40	389.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.35059053547203	4.608570744553507	54.55931789162876	17.481520828345705
2	19.112600750139656	4.559941345463251	54.577647434362774	21.749810470034316
3	21.870012369324076	3.9016987870082196	51.640805801612	22.587483042055702
4	18.939779347218895	4.947480248982523	51.11498483760275	24.997755566195835
5	16.606815098555582	17.69162477056899	43.21220872236853	22.489351408506902
6	21.304040978373635	6.724697749581039	51.78083353283856	20.19042773920677
7	83.49430911339877	2.5515471630356714	8.582465485595723	5.371678237969835
8	85.27302290320007	3.068265701061368	6.445141050195515	5.2135703455430535
9	80.90311028648951	4.836006703375628	8.89581338281063	5.365069627324235
10	41.41990363897534	27.26014484079483	15.844954512808235	15.474997007421596
11	32.27146676242917	24.149110206687414	23.946986473545607	19.6324365573378
12	29.349338640172373	22.01290798818929	29.12065577368127	19.51709759795707
13	24.57642546484718	25.99752613518474	28.925514723485758	20.500533676482323
14	19.616476139174846	28.85980268933046	28.698328146197433	22.825393025297263
15	16.950088779826032	25.435046085707448	36.423419918601866	21.191445215864654
16	19.90650686297981	23.779277392067673	33.00265341951959	23.311562325432927
17	19.33903918282659	23.561567313063602	28.35829642486633	28.741097079243477
18	20.884082475460858	22.88973346101668	32.16161419679195	24.06456986673051
19	23.933270489186818	24.91695594924587	28.71553547202937	22.434238089537946
20	26.76724223924667	25.51908766259676	28.10417664192802	19.609493456228552
21	25.190776873354082	26.20825353124252	27.501670856276434	21.099298739126965
22	23.511691006304364	24.189884087463092	27.26488308993696	25.033541816295585
23	21.941958941824275	25.89353403559173	26.511376785571784	25.653130237012213
24	22.910182746787967	23.72029865932487	30.143618825313222	23.22589976857394
25	21.366885124890274	25.9677250418961	28.25754628521267	24.407843548000958
26	20.305517317053706	29.72091712552869	26.3926711754848	23.580894381932808
27	21.17498603463411	28.979131753251934	27.519376945175967	22.326505266937993
28	18.967211315936478	28.081732303886366	30.85847099193999	22.092585388237172
29	20.15900566594845	26.63656631553747	29.821293192881654	23.383134825632432
30	22.31889913015721	27.952303287846142	28.05180651983082	21.67699106216583
31	24.00546644322081	29.198213430691883	23.3394930572181	23.456827068869206
32	23.851099273800973	30.21992957465486	24.07417105578166	21.85480009576251
33	25.33741321522624	27.907663993296623	24.33814140930492	22.416781382172214
34	21.476114236693	27.2160043093129	26.325961615194316	24.981919838799776
35	20.993935041098077	27.565263147394464	26.70364994812864	24.73715186337882
36	26.102889833213634	28.07362740403799	25.18728553188094	20.63619723086745
37	22.4765082595164	30.079901843428296	26.239301532200145	21.20428836485516
38	20.540434721889714	31.024808474982045	24.974563083552788	23.460193719575454
39	22.551821482722847	27.141439230707842	27.344186417684142	22.962552868885165
40	23.697355558215623	25.885553826510254	26.44142526534195	23.975665349932168
41	20.083817133508898	26.24079782140292	26.138426701779586	27.536958343308594
42	22.402192562445137	26.55339757401644	24.65535472029367	26.389055143244754
43	23.10083492937515	25.582679953714788	26.824350610485997	24.49213450642407
44	21.441325512728433	26.470228832495412	27.09468019312106	24.993765461655094
45	22.09208662516958	27.347428377623494	24.218937036150347	26.341547961056577
46	22.661050594525577	26.8369443779427	26.47297202936717	24.029032998164553
47	19.544654257441547	28.54857453515282	27.849807477455908	24.056963729949725
48	21.07947290719017	25.137159843587902	29.164048160561805	24.61931908866012
49	22.674766578884366	24.27105777671375	29.380261950363103	23.673913694038784
50	21.634970273721173	25.57981206607613	27.53795586944378	25.24726179075892
51	20.37995770489187	26.347533117867687	25.234543332535313	28.03796584470513
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	263.0
1	504.0
2	745.0
3	1100.0
4	1455.0
5	1168.0
6	881.0
7	974.0
8	1067.0
9	1218.0
10	1369.0
11	1397.0
12	1425.0
13	1391.0
14	1357.0
15	1583.5
16	1810.0
17	1564.0
18	1318.0
19	1421.0
20	1524.0
21	1570.0
22	1616.0
23	2359.0
24	3102.0
25	3640.5
26	5834.0
27	7489.0
28	8111.5
29	8734.0
30	10507.5
31	12281.0
32	14545.0
33	16809.0
34	18909.5
35	21010.0
36	23252.5
37	25495.0
38	27846.0
39	30197.0
40	35653.0
41	41109.0
42	47261.5
43	53414.0
44	57633.0
45	61852.0
46	67643.5
47	73435.0
48	80336.5
49	87238.0
50	82986.0
51	78734.0
52	67509.5
53	56285.0
54	49393.0
55	42501.0
56	37589.0
57	32677.0
58	32319.5
59	31962.0
60	29060.5
61	26159.0
62	24770.5
63	23382.0
64	20519.0
65	17656.0
66	14705.0
67	11754.0
68	9682.0
69	7610.0
70	5928.0
71	4246.0
72	3614.0
73	2982.0
74	2816.0
75	2019.0
76	1388.0
77	895.5
78	403.0
79	328.0
80	253.0
81	162.0
82	71.0
83	81.0
84	91.0
85	47.5
86	4.0
87	2.5
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	801984.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.29434636257286
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.8029561986382	16.47228502296073
2	12.12413632942174	5.890959346680489
3	5.455470300123237	3.9761125512576974
4	2.9534983996312727	2.870132524077871
5	1.8843956513547606	2.289008031906932
6	1.299784198475891	1.8946444508623466
7	0.9477548779330432	1.6117559689926317
8	0.7421265735396296	1.4423584017952928
9	0.578072353358943	1.2639500977616795
>10	4.310071833856488	22.649720237282164
>50	1.6476201535261266	28.33704676048836
>100	0.24742594250541425	8.887358456706131
>500	0.0036007933420746357	0.5568658147685825
>1k	0.00308639429320683	1.8578023344590997
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC	4091	0.5101099273800974	No Hit
CTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGCT	3503	0.4367917564440188	No Hit
TCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC	2855	0.3559921394940547	No Hit
GCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC	2170	0.2705789641688612	No Hit
TCCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTG	1125	0.14027711276035432	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	1122	0.13990304045966004	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4938153379618544E-4	0.0	0.0	0.47083233580719813	0.0
2	2.4938153379618544E-4	0.0	0.0	1.72921155534275	0.0
3	2.4938153379618544E-4	0.0	0.0	2.3405703854440985	0.0
4	2.4938153379618544E-4	0.0	0.0	2.962403239964887	0.0
5	3.740723006942782E-4	0.0	0.0	4.001576091293592	0.0
6	3.740723006942782E-4	0.0	0.0	4.718173928656931	0.0
7	3.740723006942782E-4	0.0	0.0	5.316564919000878	0.0
8	3.740723006942782E-4	0.0	0.0	6.220074215944457	0.0
9	3.740723006942782E-4	0.0	0.0	6.634546325113718	0.0
10	3.740723006942782E-4	0.0	0.0	7.4712213709999205	0.0
11	3.740723006942782E-4	0.0	0.0	9.168262708482962	0.0
12	3.740723006942782E-4	0.0	0.0	10.684751815497567	0.0
13	3.740723006942782E-4	0.0	0.0	11.145858271486713	0.0
14	3.740723006942782E-4	0.0	0.0	11.316809312903999	0.0
15	3.740723006942782E-4	0.0	0.0	11.589508020110127	0.0
16	3.740723006942782E-4	0.0	0.0	12.249246867767935	0.0
17	3.740723006942782E-4	0.0	0.0	13.288419719096641	0.0
18	3.740723006942782E-4	0.0	0.0	14.431335288484558	0.0
19	3.740723006942782E-4	0.0	0.0	15.099802489825233	0.0
20	3.740723006942782E-4	0.0	0.0	15.816774399489267	0.0
21	3.740723006942782E-4	0.0	0.0	16.75918721570505	0.0
22	3.740723006942782E-4	0.0	0.0	17.765940467640252	0.0
23	3.740723006942782E-4	0.0	0.0	18.679300335168783	0.0
24	3.740723006942782E-4	0.0	0.0	19.436672053307795	0.0
25	3.740723006942782E-4	0.0	0.0	20.08319367967441	0.0
26	3.740723006942782E-4	0.0	0.0	20.652656412097997	0.0
27	3.740723006942782E-4	0.0	0.0	21.245810390232226	0.0
28	3.740723006942782E-4	0.0	0.0	21.828365653180114	0.0
29	3.740723006942782E-4	0.0	0.0	22.455435519910623	0.0
30	3.740723006942782E-4	0.0	0.0	23.16480129279387	0.0
31	3.740723006942782E-4	0.0	0.0	23.8102007022584	0.0
32	3.740723006942782E-4	0.0	0.0	24.41993855239007	0.0
33	3.740723006942782E-4	0.0	0.0	24.992643244753012	0.0
34	3.740723006942782E-4	0.0	0.0	25.555871438831698	0.0
35	3.740723006942782E-4	0.0	0.0	26.209251057377703	0.0
36	3.740723006942782E-4	0.0	0.0	26.774100231426065	0.0
37	3.740723006942782E-4	0.0	0.0	27.32672971031841	0.0
38	3.740723006942782E-4	0.0	0.0	27.901803527252415	0.0
39	3.740723006942782E-4	0.0	0.0	28.509047562046124	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCACAAG	30	2.1646774E-6	45.000004	45
CGCATAC	30	2.1646774E-6	45.000004	23
TGCGATG	60	0.0	45.000004	1
TTAGTAG	30	2.1646774E-6	45.000004	44
ATCGGAT	30	2.1646774E-6	45.000004	11
AGCAACG	25	3.889747E-5	45.0	20
ACCGGTT	25	3.889747E-5	45.0	11
CGAACTG	25	3.889747E-5	45.0	27
TGCCCGG	40	6.8102963E-9	45.0	2
CTCGTTA	20	7.0320105E-4	45.0	20
CTACTTG	20	7.0320105E-4	45.0	1
CGTATGG	105	0.0	45.0	2
ATTACGA	25	3.889747E-5	45.0	19
TAAACCG	20	7.0320105E-4	45.0	26
TTCGTAC	20	7.0320105E-4	45.0	33
CGTCTAC	35	1.2113196E-7	45.0	24
CCTCGTC	55	1.8189894E-12	45.0	36
TCGATGT	35	1.2113196E-7	45.0	21
ACGGGTA	25	3.889747E-5	45.0	5
CGTTCCA	25	3.889747E-5	45.0	12
>>END_MODULE