##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935294.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	718257
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20150725993621	33.0	31.0	34.0	30.0	34.0
2	32.33992568119768	34.0	31.0	34.0	30.0	34.0
3	32.412156094545544	34.0	31.0	34.0	30.0	34.0
4	35.898203567803726	37.0	35.0	37.0	35.0	37.0
5	31.767389666929805	37.0	35.0	37.0	0.0	37.0
6	33.443653177066146	37.0	35.0	37.0	17.0	37.0
7	35.21493977782326	37.0	35.0	37.0	32.0	37.0
8	35.750405495525975	37.0	35.0	37.0	35.0	37.0
9	37.74252530779373	39.0	37.0	39.0	35.0	39.0
10	37.49131299799375	39.0	37.0	39.0	35.0	39.0
11	37.37629010228929	39.0	37.0	39.0	35.0	39.0
12	36.99825549907624	39.0	35.0	39.0	34.0	39.0
13	36.795174986112215	39.0	35.0	39.0	33.0	39.0
14	37.85777096498885	40.0	36.0	41.0	33.0	41.0
15	38.02323402347628	40.0	36.0	41.0	33.0	41.0
16	38.11666715395743	40.0	36.0	41.0	34.0	41.0
17	38.15131909608956	40.0	36.0	41.0	34.0	41.0
18	38.149223745818	40.0	36.0	41.0	34.0	41.0
19	38.18284820057445	40.0	36.0	41.0	34.0	41.0
20	38.09402205617209	40.0	36.0	41.0	34.0	41.0
21	38.03332651126268	40.0	36.0	41.0	34.0	41.0
22	37.99204184574602	40.0	36.0	41.0	34.0	41.0
23	37.965906353853846	40.0	36.0	41.0	34.0	41.0
24	37.940454461286144	40.0	36.0	41.0	34.0	41.0
25	37.85863416576518	39.0	35.0	41.0	34.0	41.0
26	37.804316560785345	39.0	35.0	41.0	34.0	41.0
27	37.73365940046529	39.0	35.0	41.0	34.0	41.0
28	37.72664241350937	39.0	36.0	41.0	33.0	41.0
29	37.68332226487177	39.0	36.0	41.0	33.0	41.0
30	37.581705434127336	39.0	36.0	41.0	33.0	41.0
31	37.31332935147169	39.0	35.0	41.0	33.0	41.0
32	37.28829792121761	39.0	35.0	41.0	33.0	41.0
33	37.25534175093316	39.0	35.0	41.0	32.0	41.0
34	37.252539132928746	39.0	35.0	41.0	32.0	41.0
35	37.1689228228893	39.0	35.0	41.0	32.0	41.0
36	37.134387830539765	39.0	35.0	41.0	32.0	41.0
37	37.06949323153133	39.0	35.0	41.0	32.0	41.0
38	37.014851230130716	39.0	35.0	41.0	32.0	41.0
39	36.894335871422065	39.0	35.0	40.0	32.0	41.0
40	36.690807050958085	39.0	35.0	40.0	31.0	41.0
41	36.60387298696706	38.0	35.0	40.0	31.0	41.0
42	36.54421606750787	38.0	35.0	40.0	31.0	41.0
43	36.35449845946506	37.0	35.0	40.0	31.0	41.0
44	36.242074911904794	37.0	35.0	40.0	31.0	41.0
45	36.106679085619774	37.0	35.0	40.0	31.0	41.0
46	35.88623013768052	37.0	34.0	40.0	31.0	41.0
47	35.713730600606745	36.0	34.0	40.0	30.0	41.0
48	35.65794416204785	36.0	34.0	40.0	31.0	41.0
49	35.590304027666974	36.0	34.0	40.0	31.0	41.0
50	35.40986861248829	35.0	34.0	40.0	31.0	41.0
51	34.784163607176815	35.0	34.0	39.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	6.0
11	7.0
12	6.0
13	8.0
14	9.0
15	26.0
16	44.0
17	38.0
18	82.0
19	162.0
20	277.0
21	459.0
22	729.0
23	1156.0
24	1709.0
25	2530.0
26	3468.0
27	4622.0
28	6047.0
29	7873.0
30	10847.0
31	14325.0
32	20173.0
33	31964.0
34	55207.0
35	82064.0
36	70039.0
37	110368.0
38	140581.0
39	153241.0
40	187.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.547680008687696	4.604758463892451	52.85197359719432	16.995587930225533
2	20.460642917507244	4.63678042817543	51.58348613379333	23.319090520523993
3	21.215386693063902	4.595848004265882	50.773608889297286	23.415156413372927
4	19.53687886090912	4.448546968564178	51.05679443430415	24.957779736222548
5	16.340251469877774	16.722008974503556	44.8534438230327	22.084295732585968
6	21.4118344826434	6.856598682644234	52.80268761738487	18.928879217327502
7	81.34107986417118	2.0782254819653687	10.69505761865182	5.885637035211631
8	81.23735654508066	2.779367273830955	8.593302954235044	7.389973226853341
9	74.59460889347407	5.902204921079781	12.127135551759329	7.376050633686828
10	40.465738586606186	25.982482593277894	18.22077612887866	15.331002691237257
11	32.319072421152875	24.262485433486898	25.2702027268791	18.14823941848113
12	29.74951862634127	20.10408530651285	30.18640959990644	19.959986467239442
13	24.05754486207583	26.932978028755723	30.44815435143688	18.561322757731563
14	17.82300764211139	30.836733926714256	29.733368418268114	21.606890012906245
15	16.468757004804687	25.56466557235084	38.68267207977089	19.283905343073577
16	16.91400153426977	22.37861935212605	37.8254580185087	22.88192109509549
17	16.989601215163933	23.15271553218416	29.882897068876463	29.974786183775443
18	19.25759164198887	23.34623957719869	33.81644731621133	23.57972146460111
19	22.939142953009856	24.77943131775952	30.15007163174184	22.13135409748878
20	25.71280196364254	25.679109288179582	28.707551753759446	19.900536994418434
21	22.73726535209542	27.809544494519372	28.324680441680343	21.12850971170486
22	19.953721300314513	25.131812150803963	28.278039754572525	26.636426794309
23	20.14724534532904	26.911954913074286	26.70617898607323	26.23462075552344
24	21.746115944571372	23.348745643968662	30.27078051449551	24.634357896964456
25	18.825016672305317	24.997459126747113	28.78732821260357	27.390195988344008
26	20.635649913610308	27.44505100542007	27.412472137410425	24.506826943559197
27	22.112140918918993	28.05931581592661	28.554960132654468	21.273583132499926
28	19.70520301229226	24.8386023387172	31.031371779182106	24.424822869808438
29	22.22380011611443	23.543522722368177	30.28762685222699	23.94505030929041
30	24.175608452127857	27.218808866464233	26.675131603311907	21.930451078096002
31	24.42677203285175	29.096131329036822	22.543741307080893	23.933355331030537
32	25.4266926740707	30.64028613713476	22.853519005035803	21.079502183758738
33	23.892840584915984	30.254491080490688	23.2096589382352	22.643009396358128
34	21.077831472578758	27.984273038759106	25.330905233085094	25.606990255577045
35	22.529401036119385	27.031828440237966	24.743232575526587	25.695537948116066
36	26.62779478654576	27.709997953378807	24.271535119045133	21.3906721410303
37	23.062914806260153	28.52349507209815	26.587001588567883	21.826588533073814
38	21.788022950002574	30.689432891012547	23.453025866785847	24.06951829219903
39	22.213775849034537	29.12328038571152	26.457103794324315	22.205839970929624
40	23.189610404075424	28.47086766992873	26.18004419031071	22.159477735685137
41	19.288360572886866	29.573119370921553	24.165166507252973	26.97335354893861
42	22.45950961842349	27.38407004735074	24.775950669467893	25.380469664757875
43	25.120813302202414	24.621549111251266	25.778655829320147	24.478981757226173
44	22.4011739530558	24.14386493970821	26.22612797369187	27.22883313354412
45	22.657628119182966	25.923033120456886	24.026636705246172	27.392702055113975
46	24.027193608972834	26.197725883632184	26.399603484546617	23.37547702284837
47	19.92030707671488	27.772231944833116	28.341387553480164	23.966073424971842
48	21.113612537016692	24.966829421780783	28.582387641192497	25.337170400010024
49	22.284502622320424	22.658045796977962	30.538929658882548	24.51852192181907
50	21.623736350637724	24.802682048347595	27.27115781677032	26.30242378424436
51	19.125605458770327	25.11983872068076	24.88468612209836	30.869869698450554
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	213.0
1	410.5
2	608.0
3	858.5
4	1109.0
5	882.5
6	656.0
7	749.0
8	842.0
9	1119.0
10	1396.0
11	1424.5
12	1453.0
13	1514.0
14	1575.0
15	1675.5
16	1776.0
17	1602.0
18	1428.0
19	1648.5
20	1869.0
21	1848.5
22	1828.0
23	1936.5
24	2045.0
25	2835.5
26	4442.5
27	5259.0
28	5849.5
29	6440.0
30	7600.0
31	8760.0
32	10334.5
33	11909.0
34	12565.0
35	13221.0
36	14646.5
37	16072.0
38	19485.0
39	22898.0
40	28483.5
41	34069.0
42	39150.5
43	44232.0
44	49688.5
45	55145.0
46	65668.0
47	76191.0
48	88519.5
49	100848.0
50	95733.0
51	90618.0
52	73913.5
53	57209.0
54	47415.5
55	37622.0
56	34004.0
57	30386.0
58	28052.5
59	25719.0
60	23565.0
61	21411.0
62	17843.0
63	14275.0
64	11779.5
65	9284.0
66	7413.0
67	5542.0
68	4549.5
69	3557.0
70	3343.5
71	3130.0
72	2587.0
73	2044.0
74	1518.0
75	813.0
76	634.0
77	405.5
78	177.0
79	158.5
80	140.0
81	90.5
82	41.0
83	23.5
84	6.0
85	4.0
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	718257.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.808632937323377
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.28713146904795	16.59162978678109
2	13.137303689418184	6.781116974126762
3	6.219390449313139	4.815418956006526
4	3.6368471023529114	3.754482076551778
5	2.334857271275952	3.0129737137700765
6	1.7135604019510013	2.6534790857931427
7	1.1982049691477283	2.1646822562687427
8	0.9051570668728736	1.8688693191637014
9	0.6339418412866964	1.4725055056841339
>10	4.624281346626309	25.587703549325514
>50	1.146470408325141	19.911372231968354
>100	0.1520331216324366	7.123103336699042
>500	0.00703356078726575	1.2156696002764418
>1k	0.003787301962373866	3.0469936075846817
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGCT	4755	0.6620193050676847	No Hit
TCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	4644	0.6465652266528554	No Hit
CCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	4526	0.6301365667163703	No Hit
GCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	3849	0.5358806109790785	No Hit
TCCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTG	1860	0.2589602328971385	No Hit
GCCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTG	1166	0.1623374363215395	No Hit
TGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTG	1021	0.14214967623009592	No Hit
TTCCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCT	849	0.11820281598369385	No Hit
TGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGCTT	845	0.11764591225703333	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCC	763	0.10622938586049283	No Hit
GTTCCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTC	744	0.1035840931588554	No Hit
CGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTG	726	0.1010780263888831	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6929274618973431	0.0
2	0.0	0.0	0.0	2.652671676015688	0.0
3	0.0	0.0	0.0	3.731951098283762	0.0
4	0.0	0.0	0.0	4.835038154866573	0.0
5	0.0	0.0	0.0	6.765127245540246	0.0
6	0.0	0.0	0.0	7.8982871033627235	0.0
7	0.0	0.0	0.0	8.955986506222702	0.0
8	0.0	0.0	0.0	10.780403114762544	0.0
9	0.0	0.0	0.0	11.596823978046855	0.0
10	0.0	0.0	0.0	13.194859221699197	0.0
11	0.0	0.0	0.0	16.025322412451253	0.0
12	0.0	0.0	0.0	18.526794726678613	0.0
13	0.0	0.0	0.0	19.301169358600056	0.0
14	0.0	0.0	0.0	19.60342885624505	0.0
15	1.3922593166512822E-4	0.0	0.0	20.029599433072008	0.0
16	1.3922593166512822E-4	0.0	0.0	21.10344904400514	0.0
17	1.3922593166512822E-4	0.0	0.0	22.682271109087694	0.0
18	1.3922593166512822E-4	0.0	0.0	24.494714287504333	0.0
19	1.3922593166512822E-4	0.0	0.0	25.568006994710807	0.0
20	1.3922593166512822E-4	0.0	0.0	26.64046434632729	0.0
21	1.3922593166512822E-4	0.0	0.0	28.06641633844153	0.0
22	1.3922593166512822E-4	0.0	0.0	29.56058903707169	0.0
23	1.3922593166512822E-4	0.0	0.0	30.915118126241723	0.0
24	1.3922593166512822E-4	0.0	0.0	31.900280818704168	0.0
25	1.3922593166512822E-4	0.0	0.0	32.7573556540347	0.0
26	1.3922593166512822E-4	0.0	0.0	33.544956749464326	0.0
27	1.3922593166512822E-4	0.0	0.0	34.319192155454104	0.0
28	1.3922593166512822E-4	0.0	0.0	35.07491051253242	0.0
29	1.3922593166512822E-4	0.0	0.0	35.90789926168488	0.0
30	1.3922593166512822E-4	0.0	0.0	36.810083298874915	0.0
31	1.3922593166512822E-4	0.0	0.0	37.71700101774156	0.0
32	1.3922593166512822E-4	0.0	0.0	38.604287880243426	0.0
33	1.3922593166512822E-4	0.0	0.0	39.338983121640304	0.0
34	1.3922593166512822E-4	0.0	0.0	40.13103944688322	0.0
35	1.3922593166512822E-4	0.0	0.0	40.95372547709246	0.0
36	1.3922593166512822E-4	0.0	0.0	41.72044268277232	0.0
37	1.3922593166512822E-4	0.0	0.0	42.55788666173807	0.0
38	1.3922593166512822E-4	0.0	0.0	43.30803598154978	0.0
39	1.3922593166512822E-4	0.0	0.0	44.04649032310162	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCA	30	2.164359E-6	45.000004	12
AATCCGG	55	1.8189894E-12	45.000004	14
ACGTTGG	30	2.164359E-6	45.000004	2
TATTGCG	60	0.0	45.000004	1
ACGATTA	30	2.164359E-6	45.000004	42
CTTTACG	75	0.0	45.000004	1
CGTTTAA	30	2.164359E-6	45.000004	18
TCGCCGA	20	7.0315175E-4	45.0	19
ACAACGA	20	7.0315175E-4	45.0	13
CCTTATG	25	3.88934E-5	45.0	1
TCGTTGC	20	7.0315175E-4	45.0	2
GTCGCAA	25	3.88934E-5	45.0	32
TCCGCAA	25	3.88934E-5	45.0	34
GGTAACC	25	3.88934E-5	45.0	13
ACGCAAT	20	7.0315175E-4	45.0	27
ACCGCAT	25	3.88934E-5	45.0	13
TAGGTGT	25	3.88934E-5	45.0	19
GACGTCC	25	3.88934E-5	45.0	24
CATTGCG	20	7.0315175E-4	45.0	10
GACGTAT	20	7.0315175E-4	45.0	8
>>END_MODULE