SRR2935293.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935293.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	832858
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC	6284	0.7545103727165975	RNA PCR Primer, Index 13 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGCT	4074	0.48915901630289915	RNA PCR Primer, Index 13 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC	3491	0.41915908834399146	Illumina Single End Adapter 1 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC	2756	0.3309087503512003	Illumina Single End Adapter 1 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTG	1565	0.18790718225675926	RNA PCR Primer, Index 13 (95% over 23bp)
GCCTCTGGAGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCC	973	0.11682663791426631	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	938	0.11262424086699054	No Hit
TTCCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCT	935	0.11226403540579546	Illumina Single End Adapter 1 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTG	924	0.11094328204808022	Illumina Single End Adapter 1 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTG	920	0.11046300809982013	RNA PCR Primer, Index 13 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGC	880	0.10566026861721926	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTAT	20	7.0321653E-4	45.000004	32
CGAAACA	20	7.0321653E-4	45.000004	44
ACGTTAG	20	7.0321653E-4	45.000004	1
CTATCGC	20	7.0321653E-4	45.000004	39
TAGGTCG	20	7.0321653E-4	45.000004	21
TTCACGA	20	7.0321653E-4	45.000004	38
CGACCTT	40	6.8102963E-9	45.000004	24
CGCGACA	20	7.0321653E-4	45.000004	13
TTATCCG	20	7.0321653E-4	45.000004	1
CACCTCG	20	7.0321653E-4	45.000004	1
CCCGTGA	20	7.0321653E-4	45.000004	24
TCCAGTA	20	7.0321653E-4	45.000004	25
TAACCGA	20	7.0321653E-4	45.000004	39
CTACGCG	20	7.0321653E-4	45.000004	27
ACTACGC	20	7.0321653E-4	45.000004	26
CATACGT	20	7.0321653E-4	45.000004	29
CTCGCCG	20	7.0321653E-4	45.000004	30
GTATCCA	20	7.0321653E-4	45.000004	9
TAAATCG	20	7.0321653E-4	45.000004	12
TTCGCTA	20	7.0321653E-4	45.000004	2