##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935292.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	796960
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28649242120056	33.0	31.0	34.0	30.0	34.0
2	32.437250301144346	34.0	31.0	34.0	30.0	34.0
3	32.49718931941377	34.0	31.0	34.0	30.0	34.0
4	35.95664148765308	37.0	35.0	37.0	35.0	37.0
5	31.562251555912468	37.0	35.0	37.0	0.0	37.0
6	33.3517240513953	37.0	35.0	37.0	17.0	37.0
7	35.13494529211002	37.0	35.0	37.0	32.0	37.0
8	35.715703423007426	37.0	35.0	37.0	35.0	37.0
9	37.75898288496286	39.0	38.0	39.0	35.0	39.0
10	37.53522510540052	39.0	37.0	39.0	35.0	39.0
11	37.42393595663521	39.0	37.0	39.0	35.0	39.0
12	37.12025195743826	39.0	37.0	39.0	34.0	39.0
13	36.96800592250552	39.0	37.0	39.0	33.0	39.0
14	38.120643445091346	40.0	37.0	41.0	33.0	41.0
15	38.253545974703876	40.0	37.0	41.0	34.0	41.0
16	38.352523338687014	40.0	37.0	41.0	34.0	41.0
17	38.34519172856856	40.0	37.0	41.0	34.0	41.0
18	38.33859792210399	40.0	37.0	41.0	34.0	41.0
19	38.388763551495686	40.0	37.0	41.0	34.0	41.0
20	38.281095663521384	40.0	37.0	41.0	34.0	41.0
21	38.20034882553704	40.0	37.0	41.0	34.0	41.0
22	38.182236247741415	40.0	37.0	41.0	34.0	41.0
23	38.1721981027906	40.0	37.0	41.0	34.0	41.0
24	38.14239359566352	40.0	36.0	41.0	34.0	41.0
25	38.05975080305159	40.0	36.0	41.0	34.0	41.0
26	37.96019875527003	40.0	36.0	41.0	34.0	41.0
27	37.88595914475005	40.0	36.0	41.0	34.0	41.0
28	37.87990237904035	40.0	36.0	41.0	34.0	41.0
29	37.83440072274644	40.0	36.0	41.0	34.0	41.0
30	37.81258908853644	40.0	36.0	41.0	34.0	41.0
31	37.558384360570166	39.0	36.0	41.0	33.0	41.0
32	37.55132252559727	39.0	35.0	41.0	33.0	41.0
33	37.58410334270227	39.0	35.0	41.0	33.0	41.0
34	37.55486222646055	39.0	35.0	41.0	33.0	41.0
35	37.47920723750251	39.0	35.0	41.0	33.0	41.0
36	37.456025396506725	39.0	35.0	41.0	33.0	41.0
37	37.376831961453526	39.0	35.0	41.0	33.0	41.0
38	37.36177223449106	39.0	35.0	41.0	33.0	41.0
39	37.35381825938567	39.0	35.0	41.0	33.0	41.0
40	37.243969584420796	39.0	35.0	41.0	33.0	41.0
41	37.16804983938968	39.0	35.0	41.0	33.0	41.0
42	37.16231178478217	39.0	35.0	41.0	33.0	41.0
43	37.12317556715519	39.0	35.0	41.0	33.0	41.0
44	37.054321421401326	39.0	35.0	41.0	32.0	41.0
45	37.03071044970889	39.0	35.0	41.0	32.0	41.0
46	36.872822977313795	39.0	35.0	41.0	32.0	41.0
47	36.79031820919494	39.0	35.0	40.0	32.0	41.0
48	36.78632930134511	39.0	35.0	40.0	32.0	41.0
49	36.79486297932142	39.0	35.0	40.0	32.0	41.0
50	36.6883783376832	38.0	35.0	40.0	32.0	41.0
51	35.94676018871713	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	12.0
11	16.0
12	18.0
13	12.0
14	14.0
15	19.0
16	30.0
17	45.0
18	96.0
19	173.0
20	252.0
21	432.0
22	678.0
23	1049.0
24	1597.0
25	2499.0
26	3613.0
27	4662.0
28	5978.0
29	7851.0
30	10213.0
31	14074.0
32	19368.0
33	29930.0
34	51853.0
35	78903.0
36	67216.0
37	106515.0
38	168580.0
39	221076.0
40	181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.373444087532626	4.4386167436257775	55.88599176872114	16.301947400120458
2	19.098449106605102	4.848424011242723	52.732006625175664	23.32112025697651
3	20.469408753262396	4.723825537040756	52.76500702670146	22.041758682995383
4	19.75770427624975	4.768746235695644	49.507628990162615	25.965920497891993
5	15.953372816703473	17.083291507729374	44.347897008632806	22.615438666934352
6	21.835725757879942	6.7765007026701465	51.964590443686006	19.423183095763903
7	80.15308171049989	3.5903934952820715	10.197124071471592	6.059400722746436
8	80.5175918490263	3.2107006625175667	9.676520778960048	6.595186709496086
9	76.27308773338687	5.988129893595664	10.976209596466573	6.762572776550893
10	41.671225657498496	24.381273840594258	17.828623770327244	16.118876731580002
11	30.040027102991367	24.5864284280265	24.931615137522588	20.441929331459548
12	27.565122465368404	21.603970086328047	30.918992170246938	19.911915278056615
13	23.773966071070067	26.171200562136114	30.49588436057017	19.55894900622365
14	18.98301043967075	28.152479421802852	31.128663922907045	21.735846215619354
15	15.102389078498293	25.072776550893394	38.68073679983939	21.14409757076892
16	19.32443284481028	23.35299136719534	33.88187612929131	23.44069965870307
17	18.569062437261593	22.572525597269625	30.2012648062638	28.657147159204975
18	20.185203774342504	22.411162417185302	33.33667938165027	24.066954426821923
19	24.264831359164827	23.252860871310983	29.39231580004015	23.08999196948404
20	25.859390684601486	24.013124874523186	29.408502308773336	20.71898213210199
21	23.26151877133106	25.720613330656494	30.01116743625778	21.00670046175467
22	22.037617948203174	24.02893495282072	28.23755270026099	25.69589439871512
23	21.247866894197955	24.01099176872114	27.258959044368602	27.48218229271231
24	21.240212808672958	24.571622164224053	29.35680586227665	24.83135916482634
25	20.103894800240916	24.497841798835577	29.017892993374822	26.380370407548686
26	19.02052800642441	29.16181489660711	27.545924513149973	24.271732583818512
27	21.9206735595262	28.500175667536638	27.971667335876333	21.607483437060832
28	18.13566552901024	27.074759084521183	31.38087231479623	23.408703071672356
29	18.503939971893193	25.042034731981527	31.34749548283477	25.1065298132905
30	22.629366593053604	25.91610620357358	27.290202770528005	24.16432443284481
31	23.386870106404338	29.179758080706687	24.305862276651276	23.127509536237703
32	22.17514053402931	30.17980827143144	24.197324834370608	23.44772636016864
33	23.187738405942582	25.6638978116844	25.610821120256976	25.537542662116042
34	20.029612527604897	26.76520778960048	27.29133206183497	25.913847620959647
35	19.97691226661313	26.36129793214214	26.5781218630797	27.08366793816503
36	24.382403131901224	25.737678177072876	25.668791407347925	24.21112728367798
37	22.130470788998196	28.212080907448307	28.120733788395903	21.5367145151576
38	19.194564344509136	29.07874924713913	26.341472595864285	25.38521381248745
39	21.593053603694038	24.416909255169646	28.62289198956033	25.367145151575986
40	24.269223047580805	22.725356354145752	27.22031218630797	25.785108411965467
41	20.106906243726158	25.974703874723954	25.735796024894597	28.18259385665529
42	21.85994278257378	26.10883858662919	24.995733788395906	27.035484842401125
43	22.402379040353342	24.295071270829148	27.28844609516161	26.01410359365589
44	19.945417586829954	24.900622364986948	28.546727564746032	26.60723248343706
45	22.02444288295523	24.148137924111623	25.737427223449107	28.08999196948404
46	23.127509536237703	25.057342903031522	28.011945392491466	23.80320216823931
47	20.124472997390082	26.620909455932544	28.883381851033928	24.371235695643445
48	20.354471993575586	24.140860269022284	29.17762497490464	26.327042762497488
49	21.276601084119655	22.002986348122867	31.071321019875526	25.64909154788195
50	21.175341296928327	23.06351636217627	28.327519574382652	27.433622766512748
51	20.205405541056013	23.411589038345713	25.673434049387673	30.709571371210597
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	337.0
1	512.0
2	687.0
3	1060.0
4	1433.0
5	1117.0
6	801.0
7	883.0
8	965.0
9	1164.5
10	1364.0
11	1438.5
12	1513.0
13	1586.0
14	1659.0
15	1589.5
16	1520.0
17	1457.0
18	1394.0
19	1436.0
20	1478.0
21	1736.0
22	1994.0
23	2518.5
24	3043.0
25	3410.5
26	4323.0
27	4868.0
28	5703.5
29	6539.0
30	8396.0
31	10253.0
32	11521.0
33	12789.0
34	15006.0
35	17223.0
36	18878.0
37	20533.0
38	23743.0
39	26953.0
40	32330.0
41	37707.0
42	45461.0
43	53215.0
44	58787.0
45	64359.0
46	72667.5
47	80976.0
48	93809.0
49	106642.0
50	98298.0
51	89954.0
52	75894.5
53	61835.0
54	51868.5
55	41902.0
56	37828.0
57	33754.0
58	30700.0
59	27646.0
60	25108.5
61	22571.0
62	19982.0
63	17393.0
64	14965.5
65	12538.0
66	10812.0
67	9086.0
68	7504.0
69	5922.0
70	5119.5
71	4317.0
72	3510.5
73	2704.0
74	2223.0
75	1286.0
76	830.0
77	586.0
78	342.0
79	258.0
80	174.0
81	188.5
82	203.0
83	109.5
84	16.0
85	11.0
86	6.0
87	3.5
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	796960.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.514001870431212
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.41916583548914	16.691047194887197
2	12.850593426003845	6.557401294144263
3	5.936913096294871	4.544232355303648
4	3.4187674358034394	3.4890575500663314
5	2.227325301937521	2.8413990959846345
6	1.5162916521997671	2.3212000830206456
7	1.115070056766705	1.9914929659805285
8	0.8520763291401741	1.739190164434605
9	0.7020403653979955	1.6120673276294402
>10	4.49480736956366	23.961883279495286
>50	1.2672236628133229	22.356110444188424
>100	0.18841097160045933	7.568010102358605
>500	0.007379019775474908	1.2720663115479123
>1k	0.002951607910189963	1.66015008414842
>5k	9.838693033966544E-4	1.3946917468101037
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	5956	0.7473398915880346	TruSeq Adapter, Index 21 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGCT	5156	0.6469584420799037	TruSeq Adapter, Index 14 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	4270	0.5357859867496486	TruSeq Adapter, Index 21 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	3139	0.39387171250752867	TruSeq Adapter, Index 21 (95% over 21bp)
TCTCTGATGGTAGCTGTGGTAGCACCTGTCTCTTATACACATCTGACGCAG	1941	0.24355049186910258	No Hit
TCCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTG	1522	0.19097570768921904	TruSeq Adapter, Index 14 (95% over 21bp)
TCTCTGATGGTAGCTGTGGTAGCACTGTGTATAACCTGTCTCTTATACACA	1340	0.16813892792611923	No Hit
GCCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTG	1015	0.12735896406344108	No Hit
TTCCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCT	967	0.12133607709295322	No Hit
CGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTG	928	0.11644248142943184	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCC	871	0.10929030315197752	No Hit
TTATGTGGGAGGTGAAAAAAATGAGCGCCCACTGCCTTACCTGTCTCTTAT	815	0.10226360168640834	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2547681188516363E-4	0.0	0.0	0.6790805059225056	0.0
2	1.2547681188516363E-4	0.0	0.0	2.487577795623369	0.0
3	1.2547681188516363E-4	0.0	0.0	3.345713712106003	0.0
4	1.2547681188516363E-4	0.0	0.0	4.231203573579602	0.0
5	1.2547681188516363E-4	0.0	0.0	5.732031720538044	0.0
6	1.2547681188516363E-4	0.0	0.0	6.748895804055411	0.0
7	1.2547681188516363E-4	0.0	0.0	7.639655691628187	0.0
8	1.2547681188516363E-4	0.0	0.0	9.005219835374422	0.0
9	1.2547681188516363E-4	0.0	0.0	9.568861674362578	0.0
10	1.2547681188516363E-4	0.0	0.0	10.757127082915078	0.0
11	1.2547681188516363E-4	0.0	0.0	13.360896406344107	0.0
12	1.2547681188516363E-4	0.0	0.0	15.46049989961855	0.0
13	1.2547681188516363E-4	0.0	0.0	16.164801244729976	0.0
14	1.2547681188516363E-4	0.0	0.0	16.395678578598677	0.0
15	1.2547681188516363E-4	0.0	0.0	16.799588436057018	0.0
16	1.2547681188516363E-4	0.0	0.0	17.768394900622365	0.0
17	1.2547681188516363E-4	0.0	0.0	19.211127283677975	0.0
18	1.2547681188516363E-4	0.0	0.0	20.87432242521582	0.0
19	1.2547681188516363E-4	0.0	0.0	21.848900823127885	0.0
20	1.2547681188516363E-4	0.0	0.0	22.877308773338687	0.0
21	1.2547681188516363E-4	0.0	0.0	24.26181991567958	0.0
22	1.2547681188516363E-4	0.0	0.0	25.544192933145954	0.0
23	1.2547681188516363E-4	0.0	0.0	26.830455731780766	0.0
24	1.2547681188516363E-4	0.0	0.0	27.81833467175266	0.0
25	1.2547681188516363E-4	0.0	0.0	28.612979321421403	0.0
26	1.2547681188516363E-4	0.0	0.0	29.67877936157398	0.0
27	1.2547681188516363E-4	0.0	0.0	30.408050592250554	0.0
28	1.2547681188516363E-4	0.0	0.0	31.151249749046375	0.0
29	1.2547681188516363E-4	0.0	0.0	31.97713812487452	0.0
30	1.2547681188516363E-4	0.0	0.0	32.8391638225256	0.0
31	1.2547681188516363E-4	0.0	0.0	33.7271632202369	0.0
32	1.2547681188516363E-4	0.0	0.0	34.5009787191327	0.0
33	1.2547681188516363E-4	0.0	0.0	35.21506725557117	0.0
34	1.2547681188516363E-4	0.0	0.0	35.97244529211002	0.0
35	1.2547681188516363E-4	0.0	0.0	36.70560630395503	0.0
36	1.2547681188516363E-4	0.0	0.0	37.68394900622365	0.0
37	1.2547681188516363E-4	0.0	0.0	38.455129492069865	0.0
38	1.2547681188516363E-4	0.0	0.0	39.185780967677175	0.0
39	1.2547681188516363E-4	0.0	0.0	39.88217727363983	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCCGA	45	3.8380676E-10	45.000004	24
TCTTACG	45	3.8380676E-10	45.000004	1
ACGGGGT	115	0.0	45.000004	5
AACGGTC	90	0.0	45.000004	44
TTTGACG	45	3.8380676E-10	45.000004	1
AACCGAT	20	7.031984E-4	45.0	38
TCGTTAG	35	1.2113014E-7	45.0	1
AGTCCGA	25	3.8897248E-5	45.0	14
TCGCACC	20	7.031984E-4	45.0	44
GTCGACT	20	7.031984E-4	45.0	18
TACCACG	20	7.031984E-4	45.0	1
CACGGGT	35	1.2113014E-7	45.0	4
AAGAGCG	20	7.031984E-4	45.0	31
GCGGTCA	20	7.031984E-4	45.0	23
TTAGCGT	20	7.031984E-4	45.0	34
CGTTGAT	20	7.031984E-4	45.0	25
ATTACCG	20	7.031984E-4	45.0	15
GATCCGA	20	7.031984E-4	45.0	39
TAAACGA	35	1.2113014E-7	45.0	14
CCTGTCG	20	7.031984E-4	45.0	20
>>END_MODULE