##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935290.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1498628
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1885257715724	33.0	31.0	34.0	30.0	34.0
2	32.345389249366754	34.0	31.0	34.0	30.0	34.0
3	32.39505801306262	34.0	31.0	34.0	30.0	34.0
4	35.891996546174234	37.0	35.0	37.0	35.0	37.0
5	31.811440197300463	37.0	35.0	37.0	0.0	37.0
6	33.48165588791882	37.0	35.0	37.0	17.0	37.0
7	35.226297653587146	37.0	35.0	37.0	32.0	37.0
8	35.75311151266358	37.0	35.0	37.0	35.0	37.0
9	37.78118652527512	39.0	38.0	39.0	35.0	39.0
10	37.41453449421738	39.0	37.0	39.0	35.0	39.0
11	37.323593980627614	39.0	37.0	39.0	34.0	39.0
12	37.21712192752304	39.0	37.0	39.0	34.0	39.0
13	37.19129830751861	39.0	37.0	39.0	34.0	39.0
14	38.34893182297408	40.0	38.0	41.0	34.0	41.0
15	38.41360364279861	40.0	38.0	41.0	34.0	41.0
16	38.47251886392087	40.0	38.0	41.0	34.0	41.0
17	38.47414301614543	40.0	38.0	41.0	34.0	41.0
18	38.447871653272195	40.0	38.0	41.0	34.0	41.0
19	38.47749474852999	40.0	38.0	41.0	34.0	41.0
20	38.408992758709964	40.0	38.0	41.0	34.0	41.0
21	38.353670824247246	40.0	38.0	41.0	34.0	41.0
22	38.33988287954049	40.0	38.0	41.0	34.0	41.0
23	38.29059846739818	40.0	37.0	41.0	34.0	41.0
24	38.2769780092191	40.0	37.0	41.0	34.0	41.0
25	38.20009969118421	40.0	37.0	41.0	34.0	41.0
26	38.17209807904297	40.0	37.0	41.0	34.0	41.0
27	38.138654155667716	40.0	37.0	41.0	34.0	41.0
28	38.08647576316471	40.0	37.0	41.0	34.0	41.0
29	38.04465084063557	40.0	37.0	41.0	34.0	41.0
30	37.97984756724151	40.0	37.0	41.0	34.0	41.0
31	37.75694635359809	40.0	37.0	41.0	33.0	41.0
32	37.805003643332434	40.0	37.0	41.0	33.0	41.0
33	37.787625081074154	40.0	37.0	41.0	33.0	41.0
34	37.760861935049924	40.0	37.0	41.0	33.0	41.0
35	37.70259463989729	40.0	37.0	41.0	33.0	41.0
36	37.69174738494143	40.0	37.0	41.0	33.0	41.0
37	37.631476924226696	40.0	36.0	41.0	33.0	41.0
38	37.62389732475304	40.0	36.0	41.0	33.0	41.0
39	37.592953688306906	39.0	36.0	41.0	33.0	41.0
40	37.574185855329006	39.0	36.0	41.0	33.0	41.0
41	37.55819923289836	40.0	36.0	41.0	33.0	41.0
42	37.529525005538396	40.0	36.0	41.0	33.0	41.0
43	37.472531542183916	39.0	36.0	41.0	33.0	41.0
44	37.42719207168157	39.0	36.0	41.0	32.0	41.0
45	37.43451343495517	39.0	36.0	41.0	32.0	41.0
46	37.31725885276399	39.0	36.0	41.0	32.0	41.0
47	37.26170870956635	39.0	35.0	41.0	32.0	41.0
48	37.24482660139808	39.0	35.0	41.0	32.0	41.0
49	37.2130074975244	39.0	35.0	41.0	32.0	41.0
50	37.14757498191679	39.0	35.0	41.0	32.0	41.0
51	36.44994021198056	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	9.0
11	7.0
12	7.0
13	11.0
14	16.0
15	31.0
16	34.0
17	62.0
18	120.0
19	236.0
20	361.0
21	694.0
22	1112.0
23	1688.0
24	2730.0
25	4261.0
26	6010.0
27	7955.0
28	10260.0
29	13650.0
30	18619.0
31	25717.0
32	35036.0
33	51440.0
34	81845.0
35	109026.0
36	129674.0
37	209254.0
38	351948.0
39	436507.0
40	304.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.793314952076166	4.6964957280926285	57.412580039876474	15.097609279954732
2	19.51191356360618	4.402226569902604	53.11111229738133	22.97474756910988
3	19.638496011018077	4.153332247896076	53.25851378727743	22.949657953808416
4	18.173289168492783	4.440594997557766	51.9715366321729	25.41457920177656
5	14.973295574352008	16.586304272975013	45.801226188220156	22.63917396445282
6	20.49107583736591	6.353477981193464	54.792450161080666	18.362996020359958
7	80.36390618619164	2.063554130845013	11.324424740495974	6.248114942467377
8	81.1305407345919	2.4503746093093146	9.204819341424288	7.214265314674489
9	75.89695374702728	4.878061800526882	12.126291514638723	7.098692937807114
10	35.662819592320446	23.955177669174738	22.287051890128836	18.094950848375984
11	25.495319719103072	26.0938671905236	26.109081106185123	22.3017319841882
12	21.627848939163023	21.752362827866556	33.849627792887894	22.770160440082527
13	22.047232535359008	24.256987057495255	34.23131023843142	19.464470168714318
14	19.14531157832364	26.055298579767626	32.433866176262555	22.36552366564618
15	17.13253722738398	26.245138887035342	34.79035491129219	21.831968974288483
16	18.16828459097254	25.16054684684925	32.22133845090309	24.449830111275116
17	17.775592074884493	25.94159457850781	30.033337159054817	26.249476187552883
18	18.864654870988666	24.705730841809974	33.535607235418	22.894007051783362
19	20.458846358135574	26.707962216107	30.474473985538776	22.358717440218655
20	21.9414691304313	27.015710369751535	30.318931716209757	20.723888783607407
21	21.081482529353515	27.760925326365182	30.682864593481508	20.4747275507998
22	19.75620367429409	26.129966876369586	28.4977993204451	25.616030128891225
23	19.04048236119971	26.840149790341567	27.78307892285477	26.336288925603952
24	20.537184678252373	24.819501570770065	29.580923351225252	25.062390399752303
25	18.611223065363784	26.766148770742305	27.75251763613118	26.870110527762726
26	17.470246118449676	26.812524522429847	30.87924421537566	24.837985143744813
27	18.443469626885392	26.31146622110357	30.25640786105691	24.988656290954125
28	15.798450315888935	26.905743119706827	31.202206284681722	26.093600279722516
29	16.646025564716528	25.256167641335942	31.171845181058945	26.92596161288859
30	19.61193838631068	25.728399576145648	28.082819752466925	26.57684228507675
31	18.28665953125125	29.862447518663735	25.215530471871606	26.635362478213402
32	18.40817067344264	29.844230856490068	25.52167716070966	26.225921309357624
33	19.100470563742302	27.5913702399795	24.342865607742546	28.965293588535644
34	17.75070264268384	26.43811539621574	27.124476521191383	28.686705439909037
35	17.274133407356594	25.450678887622548	28.046720066620935	29.228467638399923
36	17.967500940860575	27.724625457418384	26.578577205283764	27.729296396437274
37	16.785753369081586	30.29237409150236	27.380844345628137	25.541028193787916
38	17.920057545968714	31.521097964271323	25.17569403481051	25.38315045494946
39	18.467291415881725	27.381511622630832	27.019380393266374	27.13181656822107
40	20.328994253410453	22.58352306242777	28.26164998919011	28.825832694971666
41	19.932631713807563	24.421871204862047	26.44959256066215	29.195904520668236
42	20.595171049786874	26.220182727134418	27.16017584083575	26.02447038224296
43	19.560691512503436	26.26795976052763	28.622513392249445	25.548835334719488
44	18.079203111112296	25.072399554792785	27.140090803054527	29.70830653104039
45	19.507576263088637	22.985957822755214	26.574440087867035	30.932025826289113
46	20.400993442001617	22.90822005194084	28.5509145698599	28.13987193619764
47	16.503161558438787	25.339243628171904	30.83353574069082	27.324059072698493
48	17.1112510909979	24.909850876935437	29.869053560990455	28.10984447107621
49	19.62868703907841	22.96106839055456	30.539666948702415	26.870577621664616
50	19.350832895154767	21.081215618552434	28.44988883165135	31.11806265464144
51	17.948683729384477	20.572416903994853	26.049760180645233	35.42913918597544
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	220.0
1	461.0
2	702.0
3	1592.5
4	2483.0
5	1782.5
6	1082.0
7	1154.5
8	1227.0
9	1334.0
10	1441.0
11	1600.5
12	1760.0
13	1828.0
14	1896.0
15	1962.5
16	2029.0
17	2091.5
18	2154.0
19	2230.0
20	2306.0
21	2853.0
22	3400.0
23	4198.0
24	4996.0
25	5726.5
26	8960.0
27	11463.0
28	13571.0
29	15679.0
30	18380.5
31	21082.0
32	25060.0
33	29038.0
34	32057.5
35	35077.0
36	39215.5
37	43354.0
38	48492.5
39	53631.0
40	63197.5
41	72764.0
42	84354.0
43	95944.0
44	111017.0
45	126090.0
46	153814.0
47	181538.0
48	206598.0
49	231658.0
50	213627.5
51	195597.0
52	157519.5
53	119442.0
54	96084.0
55	72726.0
56	60653.0
57	48580.0
58	41911.0
59	35242.0
60	31208.0
61	27174.0
62	22791.5
63	18409.0
64	15265.5
65	12122.0
66	9687.0
67	7252.0
68	6112.5
69	4973.0
70	4050.0
71	3127.0
72	2614.0
73	2101.0
74	1651.0
75	917.5
76	634.0
77	467.0
78	300.0
79	253.5
80	207.0
81	126.5
82	46.0
83	30.5
84	15.0
85	11.5
86	8.0
87	4.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1498628.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.149938704551275
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.69117789862854	21.776585207693095
2	14.417157966916017	9.558558057941982
3	6.140125795167745	6.106343812441347
4	3.2601637409457074	4.322969127166032
5	1.9469657211497318	3.227089715798804
6	1.2760620478476439	2.5380827201612144
7	0.9081133466661186	2.1072731245136733
8	0.7101718374252686	1.8833722304276965
9	0.5528375413654164	1.6493877548854543
>10	4.831455168250414	31.049394087087308
>50	0.16322773700385176	3.7151405258860364
>100	0.09025320928776384	5.354987439905555
>500	0.006779580328750262	1.516661877873874
>1k	0.004660961476015806	2.6954371943005975
>5k	6.355856558203372E-4	1.7783466269805215
>10k+	2.1186188527344568E-4	0.7203704969368986
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGCT	10257	0.6844260216678189	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC	9589	0.6398519178875611	No Hit
CCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC	8954	0.5974798282162085	No Hit
GCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC	6778	0.45228035242902176	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCC	3890	0.25957075404970414	No Hit
TCCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG	3887	0.2593705709488946	No Hit
TTCCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCT	2398	0.16001302524709268	No Hit
CGCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG	2375	0.1584782881408862	No Hit
GCCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG	2296	0.1532067998195683	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTC	2291	0.15287316131821904	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTC	1898	0.12664917511216928	No Hit
TGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGCTT	1870	0.12478079950461356	Illumina Single End Adapter 2 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG	1843	0.1229791515973277	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7120512895795355	0.0
2	0.0	0.0	0.0	2.5246425397096544	0.0
3	0.0	0.0	0.0	3.657879073392463	0.0
4	0.0	0.0	0.0	4.906354345441297	0.0
5	0.0	0.0	0.0	7.00474033582717	0.0
6	0.0	0.0	0.0	8.437050422119432	0.0
7	6.672770026984682E-5	0.0	0.0	9.755656507151874	0.0
8	6.672770026984682E-5	0.0	0.0	11.911962141371975	0.0
9	6.672770026984682E-5	0.0	0.0	12.807914972895208	0.0
10	6.672770026984682E-5	0.0	0.0	14.68503190918627	0.0
11	6.672770026984682E-5	0.0	0.0	18.402765729720784	0.0
12	6.672770026984682E-5	0.0	0.0	21.34592440552292	0.0
13	1.3345540053969363E-4	0.0	0.0	22.26363046733412	0.0
14	1.3345540053969363E-4	0.0	0.0	22.567641869763545	0.0
15	1.3345540053969363E-4	0.0	0.0	23.036070325657867	0.0
16	1.3345540053969363E-4	0.0	0.0	24.21554915562768	0.0
17	1.3345540053969363E-4	0.0	0.0	25.969486757220604	0.0
18	1.3345540053969363E-4	0.0	0.0	27.88283683475819	0.0
19	1.3345540053969363E-4	0.0	0.0	29.174218018080538	0.0
20	1.3345540053969363E-4	0.0	0.0	30.474473985538772	0.0
21	1.3345540053969363E-4	0.0	0.0	32.3452517903042	0.0
22	1.3345540053969363E-4	0.0	0.0	34.22617220551064	0.0
23	1.3345540053969363E-4	0.0	0.0	36.162610067341596	0.0
24	2.6691080107938726E-4	0.0	0.0	37.52518970685187	0.0
25	2.6691080107938726E-4	0.0	0.0	38.715078058063774	0.0
26	2.6691080107938726E-4	0.0	0.0	39.74181718211591	0.0
27	2.6691080107938726E-4	0.0	0.0	40.75461021681164	0.0
28	2.6691080107938726E-4	0.0	0.0	41.83272967007156	0.0
29	2.6691080107938726E-4	0.0	0.0	42.92272665397951	0.0
30	2.6691080107938726E-4	0.0	0.0	44.074446760637066	0.0
31	4.003662016190809E-4	0.0	0.0	45.15636969281236	0.0
32	4.003662016190809E-4	0.0	0.0	46.2189415919094	0.0
33	4.003662016190809E-4	0.0	0.0	47.237339753427804	0.0
34	4.003662016190809E-4	0.0	0.0	48.22911356253854	0.0
35	4.003662016190809E-4	0.0	0.0	49.231296892891365	0.0
36	4.003662016190809E-4	0.0	0.0	50.20305239192114	0.0
37	4.003662016190809E-4	0.0	0.0	51.15432248696808	0.0
38	4.003662016190809E-4	0.0	0.0	52.07930186810869	0.0
39	4.003662016190809E-4	0.0	0.0	53.013556399586825	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	30	2.1659453E-6	45.000004	1
CCCTCGT	25	3.8913746E-5	45.000004	14
GTTACGG	120	0.0	45.000004	2
ATAGCCG	20	7.0339744E-4	45.0	22
TTACGGC	20	7.0339744E-4	45.0	3
CGATATC	20	7.0339744E-4	45.0	10
TATCGAG	20	7.0339744E-4	45.0	5
TACCGCG	35	1.2122655E-7	45.0	1
TACGGGT	190	0.0	42.63158	4
TACGGGA	435	0.0	42.413795	4
CGTATGG	160	0.0	42.1875	2
TTACGGG	930	0.0	41.85484	3
GGGCGTA	205	0.0	41.707317	7
TCTACGG	260	0.0	41.53846	2
CGACGGA	60	3.6379788E-12	41.250004	16
ATCCCGT	55	6.184564E-11	40.909092	11
TATGGGA	1605	0.0	40.79439	4
CGTTTTT	1760	0.0	40.78125	1
CTTTACG	95	0.0	40.263157	1
ACGGGTG	90	0.0	40.0	5
>>END_MODULE