SRR2935289.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935289.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2359577
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	10369	0.4394431713819892	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGCT	9822	0.4162610501797568	TruSeq Adapter, Index 21 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	8543	0.36205641943450034	TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	7965	0.33756050342921634	TruSeq Adapter, Index 14 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTG	3821	0.16193580459548468	TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCC	2439	0.10336598466589562	No Hit
TTCCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCT	2384	0.10103505840241706	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGACT	25	3.8920585E-5	45.000004	27
ACTATCG	20	7.0347986E-4	45.0	14
GTACCGG	20	7.0347986E-4	45.0	3
AATTCGC	20	7.0347986E-4	45.0	13
TTTACGG	985	0.0	44.314724	2
TACGGGA	1085	0.0	42.51152	4
TTACGGG	1805	0.0	42.506924	3
TTTTACG	605	0.0	42.024796	1
GTACGGG	560	0.0	41.383926	3
TTATCCG	120	0.0	41.249996	1
CTTACGG	535	0.0	41.214954	2
TCTTACG	320	0.0	40.78125	1
TTATGGG	5440	0.0	40.491726	3
GTTTACG	245	0.0	40.408165	1
ATGGGCG	775	0.0	40.064514	5
ACGGGCG	335	0.0	39.62686	5
TTCTACG	250	0.0	39.600002	1
GCGATAT	165	0.0	39.545452	9
CTTATGG	1270	0.0	39.507874	2
TATGGGA	3060	0.0	39.485294	4