##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935289.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2359577
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27786463421198	33.0	31.0	34.0	30.0	34.0
2	32.41813426728604	34.0	31.0	34.0	30.0	34.0
3	32.47479230387481	34.0	31.0	34.0	30.0	34.0
4	35.95161208979406	37.0	35.0	37.0	35.0	37.0
5	31.563827330068058	37.0	35.0	37.0	0.0	37.0
6	33.38662522986112	37.0	35.0	37.0	17.0	37.0
7	35.25564878789715	37.0	35.0	37.0	32.0	37.0
8	35.82146376236079	37.0	35.0	37.0	35.0	37.0
9	37.88795237451458	39.0	38.0	39.0	35.0	39.0
10	37.4786391798191	39.0	37.0	39.0	35.0	39.0
11	37.35196435632319	39.0	37.0	39.0	34.0	39.0
12	37.227399656802895	39.0	37.0	39.0	34.0	39.0
13	37.22065310858684	39.0	37.0	39.0	34.0	39.0
14	38.41396063786009	40.0	38.0	41.0	34.0	41.0
15	38.48155961852485	40.0	38.0	41.0	34.0	41.0
16	38.537927772647386	40.0	38.0	41.0	34.0	41.0
17	38.535577351364246	40.0	38.0	41.0	34.0	41.0
18	38.50522784380421	40.0	38.0	41.0	34.0	41.0
19	38.525174639352734	40.0	38.0	41.0	34.0	41.0
20	38.44817143072678	40.0	38.0	41.0	34.0	41.0
21	38.396708816876924	40.0	38.0	41.0	34.0	41.0
22	38.382376586989956	40.0	38.0	41.0	34.0	41.0
23	38.343590821575226	40.0	37.0	41.0	34.0	41.0
24	38.3181735539887	40.0	37.0	41.0	34.0	41.0
25	38.256997758496546	40.0	37.0	41.0	34.0	41.0
26	38.22960598446247	40.0	37.0	41.0	34.0	41.0
27	38.19292440975649	40.0	37.0	41.0	34.0	41.0
28	38.1945361393165	40.0	37.0	41.0	34.0	41.0
29	38.16255795000544	40.0	37.0	41.0	34.0	41.0
30	38.09904317595908	40.0	37.0	41.0	34.0	41.0
31	37.90152641765876	40.0	37.0	41.0	33.0	41.0
32	37.93863900182109	40.0	37.0	41.0	34.0	41.0
33	37.95920200951272	40.0	37.0	41.0	34.0	41.0
34	37.927193306257855	40.0	37.0	41.0	34.0	41.0
35	37.875582784541464	40.0	37.0	41.0	33.0	41.0
36	37.884601773962025	40.0	37.0	41.0	33.0	41.0
37	37.85596486149848	40.0	37.0	41.0	33.0	41.0
38	37.84646866790107	40.0	37.0	41.0	33.0	41.0
39	37.84066423770023	40.0	37.0	41.0	33.0	41.0
40	37.76704722922795	40.0	36.0	41.0	33.0	41.0
41	37.689727014630165	40.0	36.0	41.0	33.0	41.0
42	37.69092172029139	40.0	36.0	41.0	33.0	41.0
43	37.68757239115316	40.0	36.0	41.0	33.0	41.0
44	37.66975690981901	40.0	36.0	41.0	33.0	41.0
45	37.66713355826065	40.0	36.0	41.0	33.0	41.0
46	37.545016755121786	40.0	36.0	41.0	33.0	41.0
47	37.50049224924637	39.0	36.0	41.0	33.0	41.0
48	37.471374318363	39.0	35.0	41.0	33.0	41.0
49	37.4802542998173	39.0	36.0	41.0	33.0	41.0
50	37.4207660949399	39.0	35.0	41.0	33.0	41.0
51	36.79544130155532	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	5.0
10	5.0
11	15.0
12	15.0
13	20.0
14	16.0
15	38.0
16	60.0
17	106.0
18	196.0
19	293.0
20	593.0
21	964.0
22	1670.0
23	2726.0
24	4158.0
25	6397.0
26	8861.0
27	11580.0
28	14936.0
29	20039.0
30	26759.0
31	36900.0
32	51167.0
33	76477.0
34	125005.0
35	174273.0
36	195820.0
37	313713.0
38	520374.0
39	765724.0
40	668.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.441828768461466	4.535897747774284	59.38827171141268	12.634001772351569
2	19.7284513283525	4.011608860401673	54.447555642388444	21.812384168857385
3	20.105213773485673	3.9354511423022007	53.53336636185214	22.425968722359983
4	18.683603035628842	4.313061196985731	52.575482808995	24.42785295839042
5	15.299310003445532	17.22715554525239	45.759769653628595	21.71376479767348
6	20.359708540980016	6.13300604303229	55.1998938792843	18.307391536703403
7	84.3051106194034	1.9068248249580328	8.880447639555735	4.90761691608284
8	86.81967149196656	1.859782494913283	6.4522581801738195	4.868287832946329
9	82.3788331552647	4.044877535253141	8.566408301148892	5.009881008333273
10	37.740917122009584	25.446467735530565	19.679332354909377	17.13328278755048
11	26.443044664361455	26.697327529468208	25.141243536447423	21.71838426972292
12	22.2835703179002	23.79820620390858	31.754547531188855	22.163675947002364
13	22.210930179434705	25.201974760730415	32.90992410927891	19.67717095055597
14	21.05169697789053	26.33997534303818	31.275478613327728	21.332849065743563
15	19.15707773045762	26.741233704176636	33.217182571282905	20.88450599408284
16	20.304020593521635	25.817805479541462	31.15045620465024	22.727717722286663
17	20.600048228983415	26.39341712518812	27.879191906006884	25.127342739821586
18	21.672062407795973	25.24418571633814	31.329852765983055	21.75389910988283
19	22.45499934945967	26.98920187813324	29.1340354648312	21.42176330757589
20	23.508620401029507	27.992220639546833	28.862503745374703	19.636655214048957
21	23.370544805276538	27.933311775797105	28.821309921227407	19.87483349769895
22	22.16825303857429	25.380608473467912	28.28795161166599	24.16318687629181
23	20.608312422099385	26.31552180751041	27.58248618290482	25.493679587485385
24	21.103274019029683	26.23326130064838	29.15798043462875	23.50548424569319
25	20.063723285995753	27.140754465736865	27.350792112315048	25.44473013595233
26	19.19166020011214	28.04083104725974	28.731887113664868	24.035621638963256
27	19.31621642353693	26.46915103851241	30.96415162548202	23.250480912468632
28	17.654647421974364	24.961507931294467	32.720483374774375	24.663361271956795
29	18.783536201615796	23.4219099440281	32.27875165760643	25.51580219674967
30	21.444987809255643	25.465242287070943	31.141429162938948	21.948340740734462
31	21.613916392641563	27.077607554235357	28.760112511691716	22.548363541431364
32	20.104832349188012	28.55477909811801	27.643132646232775	23.6972559064612
33	21.540767688445854	25.73592639697709	28.376569190155692	24.34673672442137
34	19.90344032002346	25.627135711188913	28.941712857855457	25.527711110932174
35	18.340533070122316	25.44248397064389	28.89089866531162	27.326084293922175
36	20.87352945040573	24.984732432974216	29.760842727319346	24.38089538930071
37	19.050999395230587	26.374261149350076	32.53494164420148	22.03979781121786
38	18.762218821424348	27.437629710749	31.00496402533166	22.795187442494992
39	22.084721117386717	24.317960380186786	30.369934950205057	23.227383552221436
40	23.56655451379633	24.590805894446337	28.21213293738666	23.630506654370677
41	20.11792791674101	26.2452125953084	26.762381562458014	26.874477925492577
42	18.99348908723894	25.23554009892451	28.489470782263094	27.281500031573458
43	19.511548044416436	23.99730968728717	30.46914764807421	26.021994620222184
44	19.309562688566636	23.359441120166878	30.237750240827065	27.09324595043942
45	21.430069881169377	22.807308259065078	28.41026166978234	27.352360189983205
46	22.226610956116286	23.771887927370035	29.768047408497374	24.23345370801631
47	18.780145763414374	24.44997556765471	31.861346334533692	24.908532334397222
48	18.312265291617948	23.745145846056307	32.48175414491665	25.4608347174091
49	20.935659230446813	21.6754528459974	33.046770671183864	24.342117252371928
50	21.55657560655999	21.493682977923587	30.581328772063802	26.368412643452622
51	20.406327066249585	21.898543679651056	27.881734734657947	29.81339451944141
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	344.0
1	720.5
2	1097.0
3	2157.0
4	3217.0
5	2430.0
6	1643.0
7	1800.0
8	1957.0
9	2162.0
10	2367.0
11	2780.0
12	3193.0
13	3305.5
14	3418.0
15	3495.0
16	3572.0
17	3553.5
18	3535.0
19	4324.0
20	5113.0
21	5356.0
22	5599.0
23	7168.0
24	8737.0
25	11364.0
26	17315.0
27	20639.0
28	22182.0
29	23725.0
30	30790.0
31	37855.0
32	42165.5
33	46476.0
34	55069.0
35	63662.0
36	71421.0
37	79180.0
38	90802.0
39	102424.0
40	116112.0
41	129800.0
42	143952.0
43	158104.0
44	178954.5
45	199805.0
46	229616.5
47	259428.0
48	285836.5
49	312245.0
50	293072.5
51	273900.0
52	228861.0
53	183822.0
54	151801.5
55	119781.0
56	103527.0
57	87273.0
58	76126.0
59	64979.0
60	56706.5
61	48434.0
62	41183.0
63	33932.0
64	28734.0
65	23536.0
66	18711.0
67	13886.0
68	10712.0
69	7538.0
70	6138.0
71	4738.0
72	3830.5
73	2923.0
74	2331.0
75	1584.0
76	1429.0
77	886.0
78	343.0
79	206.0
80	69.0
81	40.5
82	12.0
83	29.5
84	47.0
85	27.5
86	8.0
87	35.0
88	62.0
89	31.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2359577.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.269051835960422
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.769670346369836	16.75168775890897
2	14.276541301955534	7.500624069985997
3	6.677548340129966	5.262385904520166
4	3.6786022499309885	3.865335727492711
5	2.217639077151446	2.912763793557138
6	1.5139640513089663	2.3862240084969524
7	1.022418758054136	1.8800579947367662
8	0.7416092409914137	1.5585097274904567
9	0.5834778473164253	1.37946688346608
>10	4.243687051506704	24.108870186218702
>50	1.0655556609070678	19.472838197834268
>100	0.19610870869830177	8.131426255502202
>500	0.00849593313531818	1.4365966906348138
>1k	0.003987886981884043	1.6816848867067815
>5k	5.201591715500926E-4	1.1992514778990726
>10k+	1.7338639051669752E-4	0.4722764365490119
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	10369	0.4394431713819892	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGCT	9822	0.4162610501797568	TruSeq Adapter, Index 21 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	8543	0.36205641943450034	TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	7965	0.33756050342921634	TruSeq Adapter, Index 14 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTG	3821	0.16193580459548468	TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCC	2439	0.10336598466589562	No Hit
TTCCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCT	2384	0.10103505840241706	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.238047751779238E-5	0.0	0.0	0.4290599543901301	0.0
2	4.238047751779238E-5	0.0	0.0	1.6407178066238144	0.0
3	4.238047751779238E-5	0.0	0.0	2.3399956856673887	0.0
4	8.476095503558476E-5	0.0	0.0	3.167686411589874	0.0
5	8.476095503558476E-5	0.0	0.0	4.572472099872138	0.0
6	8.476095503558476E-5	0.0	0.0	5.5568858316554195	0.0
7	8.476095503558476E-5	0.0	0.0	6.386907483841384	0.0
8	8.476095503558476E-5	0.0	0.0	7.741896111040242	0.0
9	8.476095503558476E-5	0.0	0.0	8.301403175230137	0.0
10	8.476095503558476E-5	0.0	0.0	9.5549329392514	0.0
11	8.476095503558476E-5	0.0	0.0	12.152940972047109	0.0
12	8.476095503558476E-5	0.0	0.0	14.232296720980074	0.0
13	1.2714143255337714E-4	0.0	0.0	14.876225696385411	0.0
14	1.2714143255337714E-4	0.0	0.0	15.072955873023004	0.0
15	1.2714143255337714E-4	0.0	0.0	15.37233156620869	0.0
16	1.2714143255337714E-4	0.0	0.0	16.23680854661662	0.0
17	1.6952191007116953E-4	0.0	0.0	17.613538358782105	0.0
18	1.6952191007116953E-4	0.0	0.0	19.101516924431795	0.0
19	1.6952191007116953E-4	0.0	0.0	20.123776422638464	0.0
20	1.6952191007116953E-4	0.0	0.0	21.169811368732617	0.0
21	2.119023875889619E-4	0.0	0.0	22.720640182541192	0.0
22	2.119023875889619E-4	0.0	0.0	24.302449125415276	0.0
23	2.119023875889619E-4	0.0	0.0	26.033945914882203	0.0
24	2.119023875889619E-4	0.0	0.0	27.248485639587095	0.0
25	2.119023875889619E-4	0.0	0.0	28.33211206923953	0.0
26	2.119023875889619E-4	0.0	0.0	29.262363550755072	0.0
27	2.119023875889619E-4	0.0	0.0	30.150827881438072	0.0
28	2.119023875889619E-4	0.0	0.0	31.149354312234777	0.0
29	2.119023875889619E-4	0.0	0.0	32.15262735651348	0.0
30	2.966633426245467E-4	0.0	0.0	33.22582818869653	0.0
31	2.966633426245467E-4	0.0	0.0	34.21778564547798	0.0
32	2.966633426245467E-4	0.0	0.0	35.17702537361569	0.0
33	2.966633426245467E-4	0.0	0.0	36.1221947832175	0.0
34	2.966633426245467E-4	0.0	0.0	37.05278530855318	0.0
35	2.966633426245467E-4	0.0	0.0	37.99825138149762	0.0
36	2.966633426245467E-4	0.0	0.0	38.90137935740177	0.0
37	2.966633426245467E-4	0.0	0.0	39.822561416728504	0.0
38	2.966633426245467E-4	0.0	0.0	40.73200408378281	0.0
39	2.966633426245467E-4	0.0	0.0	41.679419658693064	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGACT	25	3.8920585E-5	45.000004	27
ACTATCG	20	7.0347986E-4	45.0	14
GTACCGG	20	7.0347986E-4	45.0	3
AATTCGC	20	7.0347986E-4	45.0	13
TTTACGG	985	0.0	44.314724	2
TACGGGA	1085	0.0	42.51152	4
TTACGGG	1805	0.0	42.506924	3
TTTTACG	605	0.0	42.024796	1
GTACGGG	560	0.0	41.383926	3
TTATCCG	120	0.0	41.249996	1
CTTACGG	535	0.0	41.214954	2
TCTTACG	320	0.0	40.78125	1
TTATGGG	5440	0.0	40.491726	3
GTTTACG	245	0.0	40.408165	1
ATGGGCG	775	0.0	40.064514	5
ACGGGCG	335	0.0	39.62686	5
TTCTACG	250	0.0	39.600002	1
GCGATAT	165	0.0	39.545452	9
CTTATGG	1270	0.0	39.507874	2
TATGGGA	3060	0.0	39.485294	4
>>END_MODULE