##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935287.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1280415
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23784319927523	33.0	31.0	34.0	30.0	34.0
2	32.38691674183761	34.0	31.0	34.0	30.0	34.0
3	32.443544475814484	34.0	31.0	34.0	30.0	34.0
4	35.92886837470664	37.0	35.0	37.0	35.0	37.0
5	31.763176001530752	37.0	35.0	37.0	0.0	37.0
6	33.49651323984802	37.0	35.0	37.0	17.0	37.0
7	35.273198142789646	37.0	35.0	37.0	32.0	37.0
8	35.81130258548986	37.0	35.0	37.0	35.0	37.0
9	37.85815302070032	39.0	38.0	39.0	35.0	39.0
10	37.47289902102053	39.0	37.0	39.0	35.0	39.0
11	37.361551528215465	39.0	37.0	39.0	34.0	39.0
12	37.23376249106735	39.0	37.0	39.0	34.0	39.0
13	37.17677003159132	39.0	37.0	39.0	34.0	39.0
14	38.291195432730795	40.0	38.0	41.0	34.0	41.0
15	38.380864016744574	40.0	38.0	41.0	34.0	41.0
16	38.454070750498865	40.0	38.0	41.0	34.0	41.0
17	38.47195089092208	40.0	38.0	41.0	34.0	41.0
18	38.457315792145515	40.0	38.0	41.0	34.0	41.0
19	38.50077201532316	40.0	38.0	41.0	34.0	41.0
20	38.414445316557526	40.0	38.0	41.0	34.0	41.0
21	38.36558303362582	40.0	37.0	41.0	34.0	41.0
22	38.33480707426889	40.0	37.0	41.0	34.0	41.0
23	38.2826224310087	40.0	37.0	41.0	34.0	41.0
24	38.23512064447855	40.0	37.0	41.0	34.0	41.0
25	38.15091044700351	40.0	37.0	41.0	34.0	41.0
26	38.109492625437845	40.0	37.0	41.0	34.0	41.0
27	38.08449448030521	40.0	37.0	41.0	34.0	41.0
28	38.099578652233845	40.0	37.0	41.0	34.0	41.0
29	38.09208576906706	40.0	37.0	41.0	34.0	41.0
30	38.058310001054345	40.0	37.0	41.0	34.0	41.0
31	37.865984856472316	40.0	37.0	41.0	34.0	41.0
32	37.91811561095426	40.0	37.0	41.0	34.0	41.0
33	37.89194675163912	40.0	37.0	41.0	34.0	41.0
34	37.90211142481149	40.0	37.0	41.0	34.0	41.0
35	37.837397250110314	40.0	36.0	41.0	33.0	41.0
36	37.8210049085648	40.0	36.0	41.0	33.0	41.0
37	37.813989214434386	40.0	36.0	41.0	33.0	41.0
38	37.80857456371567	40.0	36.0	41.0	33.0	41.0
39	37.78232682372512	40.0	36.0	41.0	33.0	41.0
40	37.73886981955069	40.0	36.0	41.0	33.0	41.0
41	37.671533838638254	40.0	36.0	41.0	33.0	41.0
42	37.619095371422546	40.0	36.0	41.0	33.0	41.0
43	37.56145077963004	40.0	36.0	41.0	33.0	41.0
44	37.54407360113713	39.0	36.0	41.0	33.0	41.0
45	37.56161791294229	39.0	36.0	41.0	33.0	41.0
46	37.45446593487268	39.0	35.0	41.0	33.0	41.0
47	37.39617780172835	39.0	35.0	41.0	33.0	41.0
48	37.407151587571214	39.0	35.0	41.0	33.0	41.0
49	37.41847994595502	39.0	35.0	41.0	33.0	41.0
50	37.352453696653036	39.0	35.0	41.0	33.0	41.0
51	36.73426271950891	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	14.0
11	21.0
12	13.0
13	15.0
14	22.0
15	31.0
16	35.0
17	45.0
18	88.0
19	169.0
20	271.0
21	507.0
22	829.0
23	1349.0
24	2055.0
25	3234.0
26	4522.0
27	5945.0
28	7778.0
29	10461.0
30	14671.0
31	20391.0
32	28549.0
33	42898.0
34	70146.0
35	105009.0
36	108984.0
37	170138.0
38	284093.0
39	397834.0
40	289.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.928437264480657	4.420363710203333	59.93392767188763	13.717271353428382
2	18.203238793672362	4.180988195233577	54.855183670919196	22.760589340174864
3	18.55367205163951	4.017135069489189	54.79653081227571	22.632662066595596
4	18.26282884845929	4.11796175458738	52.31124283923572	25.307966557717616
5	15.312379189559636	15.979506644330158	46.82247552551321	21.885638640596994
6	19.610673102080185	5.457449342595956	55.09549638203238	19.83638117329147
7	82.08057543843206	1.4683520577312825	10.781270135073393	5.669802368763253
8	83.28268569174838	1.525286723445133	8.138377010578601	7.053650574227888
9	78.6269295501849	4.661223119066865	10.588520128239672	6.123327202508562
10	35.11431840457976	33.55060663925368	16.90694032794055	14.428134628226005
11	23.827821448514737	26.155582369778546	29.083148822842592	20.933447358864118
12	22.663823838364905	22.345645747667746	33.478754934923444	21.511775479043905
13	21.8825146534522	26.483288621267327	32.95205070231136	18.682146022969114
14	18.263922243959964	28.641963738319216	31.574450471136313	21.519663546584507
15	15.038639815997158	27.68336828293951	35.82369778548361	21.454294115579714
16	17.130852106543582	27.58769617663023	32.09568772624501	23.18576399058118
17	17.916925371852095	26.654014518730257	28.713190645220493	26.715869464197155
18	18.322262703888974	25.26212204636778	33.81919143402725	22.596423815715998
19	19.353334661027873	26.316389608056763	31.47448288250294	22.855792848412428
20	20.540371676370555	29.39765622864462	30.426228996067685	19.635743098917146
21	21.32863173268042	28.89469429833296	30.455438275871494	19.321235693115124
22	19.892769141254984	26.680958907854098	28.782933658227993	24.643338292662925
23	18.395051604362646	27.69164684887322	27.464611083125394	26.44869046363874
24	20.183612344435204	26.892843335949674	29.162419996641713	23.761124322973412
25	17.86202129778236	28.111432621454764	27.446101459292493	26.580444621470384
26	17.310637566726413	28.3940753583799	29.8364983228094	24.458788752084285
27	18.312500244061496	27.521233350124763	31.934411889895074	22.231854515918666
28	14.967959606846218	24.70808292623876	32.757348203512144	27.56660926340288
29	15.737944338359048	22.778474166578803	32.757816801583864	28.725764693478283
30	18.438787424389748	24.999550926847935	30.586958134667274	25.974703514095037
31	18.968225145753525	27.33723050729646	25.556557834764508	28.137986512185503
32	17.097737842808776	27.440946880503585	27.519749456230986	27.94156582045665
33	17.727065053127305	26.872615519187136	26.674164235814168	28.726155191871385
34	15.831507753345594	24.24463943330873	29.11228000296779	30.811572810377886
35	14.519511252211197	24.049312137080555	31.638179808890087	29.792996801818163
36	17.49620240312711	25.04672313273431	32.303979569124074	25.153094895014505
37	15.419844347340511	25.614273497264563	31.903796815876102	27.062085339518827
38	15.406489302296523	26.85410589535424	27.135030439349745	30.604374362999497
39	16.860002421090037	25.47814575743021	28.1387675089717	29.523084312508054
40	19.39316549712398	23.049245752353727	28.741540828559494	28.816047921962802
41	16.337203172408945	24.290093446265466	30.267374249754965	29.105329131570624
42	17.970345552028054	25.03422718415514	31.048683434667666	25.946743829149142
43	19.531480027959685	24.81281459526794	29.97778064143266	25.67792473533971
44	17.867644474642987	23.434355267628074	28.19671747050761	30.501282787221328
45	18.837954881815662	21.69359153087085	27.322860166430413	32.145593420883074
46	20.244451994080045	21.797073605042115	30.99104587184624	26.9674285290316
47	14.582928191250494	24.0026085292659	33.58770398659809	27.826759292885512
48	15.524107418297975	22.810573134491552	32.349199283044946	29.316120164165525
49	18.542972395668592	20.280846444316882	33.353951648488966	27.82222951152556
50	18.659965714241086	19.861138771413955	30.279323500583793	31.199572013761163
51	16.30807199228375	19.984380064276035	27.711015569170932	35.99653237426928
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	224.0
1	370.5
2	517.0
3	1011.0
4	1505.0
5	1131.5
6	758.0
7	959.0
8	1160.0
9	1779.5
10	2399.0
11	3003.0
12	3607.0
13	4011.5
14	4416.0
15	4249.0
16	4082.0
17	3845.0
18	3608.0
19	3426.0
20	3244.0
21	2980.5
22	2717.0
23	3778.0
24	4839.0
25	5471.5
26	7710.0
27	9316.0
28	10274.0
29	11232.0
30	14319.0
31	17406.0
32	20367.0
33	23328.0
34	25857.5
35	28387.0
36	33099.0
37	37811.0
38	44671.5
39	51532.0
40	60045.0
41	68558.0
42	77460.0
43	86362.0
44	101711.0
45	117060.0
46	140203.5
47	163347.0
48	182755.0
49	202163.0
50	184264.5
51	166366.0
52	130893.5
53	95421.0
54	75892.5
55	56364.0
56	46071.5
57	35779.0
58	30441.5
59	25104.0
60	21657.5
61	18211.0
62	15107.5
63	12004.0
64	9657.5
65	7311.0
66	5317.5
67	3324.0
68	2677.0
69	2030.0
70	1686.5
71	1343.0
72	955.0
73	567.0
74	466.0
75	297.5
76	230.0
77	200.5
78	171.0
79	127.0
80	83.0
81	69.0
82	55.0
83	30.0
84	5.0
85	2.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1280415.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.395331598748164
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.95876341312494	18.72920958959061
2	13.266397089734014	7.534074889673299
3	6.230689595971217	5.307674914994188
4	3.4125498768023745	3.8760194139628252
5	2.200808102749876	3.1246337931397274
6	1.4443048132404095	2.460690846097768
7	1.0661939576244441	2.1192451682729416
8	0.7678176506652957	1.7441949438410282
9	0.5970294298466801	1.5257563771237233
>10	4.151812289226713	24.700036276380224
>50	0.7367161302205303	14.102446477179578
>100	0.15069336517335225	7.828361821372256
>500	0.010449539891076004	2.0144039053802327
>1k	0.004674794161797159	2.193684135117151
>5k	8.249636756112634E-4	1.9448313075162982
>10k+	2.7498789187042113E-4	0.7947361403581676
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	10178	0.7948985289925532	TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	9656	0.7541304967530058	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGCT	8425	0.6579897923720044	TruSeq Adapter, Index 16 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	6826	0.5331084062589082	TruSeq Adapter, Index 13 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTG	3750	0.2928737948243343	TruSeq Adapter, Index 16 (95% over 22bp)
TTCCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCT	2533	0.19782648594401034	No Hit
TGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTG	2060	0.1608853379568343	TruSeq Adapter, Index 16 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTG	1905	0.14877988777076182	No Hit
TTCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTG	1748	0.13651823822744968	TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCC	1723	0.13456574626195414	No Hit
TGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGCTT	1712	0.1337066497971361	TruSeq Adapter, Index 13 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTC	1631	0.12738057582893048	No Hit
TTTCCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTC	1558	0.12167929928968342	No Hit
CGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTG	1324	0.10340397449264496	TruSeq Adapter, Index 16 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5619935723964497E-4	0.0	0.0	0.67751471202696	0.0
2	1.5619935723964497E-4	0.0	0.0	2.8836744336797056	0.0
3	1.5619935723964497E-4	0.0	0.0	4.071726744844445	0.0
4	1.5619935723964497E-4	0.0	0.0	5.427849564399042	0.0
5	1.5619935723964497E-4	0.0	0.0	7.658142086745313	0.0
6	1.5619935723964497E-4	0.0	0.0	9.047457269713336	0.0
7	1.5619935723964497E-4	0.0	0.0	10.348676015198198	0.0
8	1.5619935723964497E-4	0.0	0.0	12.47728275598146	0.0
9	1.5619935723964497E-4	0.0	0.0	13.369181085819832	0.0
10	1.5619935723964497E-4	0.0	0.0	15.106117938324683	0.0
11	1.5619935723964497E-4	0.0	0.0	18.76821186880816	0.0
12	1.5619935723964497E-4	0.0	0.0	21.93046785612477	0.0
13	1.5619935723964497E-4	0.0	0.0	22.814478118422542	0.0
14	1.5619935723964497E-4	0.0	0.0	23.066974379400428	0.0
15	1.5619935723964497E-4	0.0	0.0	23.433886669556355	0.0
16	1.5619935723964497E-4	0.0	0.0	24.40365037897869	0.0
17	1.5619935723964497E-4	0.0	0.0	25.97142332759301	0.0
18	1.5619935723964497E-4	0.0	0.0	27.715154852137783	0.0
19	1.5619935723964497E-4	0.0	0.0	28.88235454911103	0.0
20	1.5619935723964497E-4	0.0	0.0	30.04463396633123	0.0
21	1.5619935723964497E-4	0.0	0.0	31.662312609583612	0.0
22	1.5619935723964497E-4	0.0	0.0	33.38183323375624	0.0
23	1.5619935723964497E-4	0.0	0.0	34.954448362444985	0.0
24	1.5619935723964497E-4	0.0	0.0	36.20052873482425	0.0
25	1.5619935723964497E-4	0.0	0.0	37.217074151739865	0.0
26	1.5619935723964497E-4	0.0	0.0	38.161611664968	0.0
27	1.5619935723964497E-4	0.0	0.0	39.07077002378135	0.0
28	1.5619935723964497E-4	0.0	0.0	39.978834987094025	0.0
29	1.5619935723964497E-4	0.0	0.0	40.96140704381001	0.0
30	1.5619935723964497E-4	0.0	0.0	42.04558678241039	0.0
31	3.9049839309911237E-4	0.0	0.0	42.98918709949508	0.0
32	3.9049839309911237E-4	0.0	0.0	43.87757094379557	0.0
33	3.9049839309911237E-4	0.0	0.0	44.71230030888423	0.0
34	3.9049839309911237E-4	0.0	0.0	45.59638867086062	0.0
35	3.9049839309911237E-4	0.0	0.0	46.591847174548874	0.0
36	3.9049839309911237E-4	0.0	0.0	47.47484214102459	0.0
37	3.9049839309911237E-4	0.0	0.0	48.299262348535436	0.0
38	3.9049839309911237E-4	0.0	0.0	49.153672832636296	0.0
39	4.6859807171893486E-4	0.0	0.0	50.015737085241895	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAACGG	30	2.165696E-6	45.000004	2
CTATGCG	35	1.2120654E-7	45.0	1
CAGCGGT	20	7.033588E-4	45.0	35
AGTCCGT	20	7.033588E-4	45.0	42
TCCGCCG	25	3.8910555E-5	45.0	15
CCGATAT	25	3.8910555E-5	45.0	25
CCCTACG	20	7.033588E-4	45.0	38
GTTTACG	25	3.8910555E-5	45.0	1
ACACCGC	20	7.033588E-4	45.0	33
CTTCACG	35	1.2120654E-7	45.0	1
TTCGTAA	25	3.8910555E-5	45.0	22
CGTCTAC	20	7.033588E-4	45.0	43
TTCCGCG	110	0.0	45.0	1
TACGGGT	55	1.8189894E-12	45.0	4
CACTGCG	20	7.033588E-4	45.0	1
ACTTACG	20	7.033588E-4	45.0	1
GCGTTAC	20	7.033588E-4	45.0	43
ACGGGTC	25	3.8910555E-5	45.0	5
TTATCCG	25	3.8910555E-5	45.0	1
CCGTCCA	20	7.033588E-4	45.0	19
>>END_MODULE