SRR2935285.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935285.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	980452
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	6640	0.6772386613521111	Illumina PCR Primer Index 5 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGCT	5404	0.5511743563172904	Illumina PCR Primer Index 5 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	4503	0.4592779656729753	Illumina PCR Primer Index 5 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	3446	0.3514705462378576	Illumina PCR Primer Index 5 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTG	1712	0.17461334160162864	Illumina PCR Primer Index 5 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGC	1683	0.17165552214692814	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCG	1599	0.16308804510572675	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTG	1481	0.1510527797383248	Illumina PCR Primer Index 5 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTC	1436	0.14646305989482403	No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1119	0.11413103344171872	No Hit
TGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTG	1097	0.11188717040711835	Illumina PCR Primer Index 5 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTG	1081	0.11025527001831809	Illumina PCR Primer Index 5 (95% over 22bp)
TTCCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCT	1066	0.10872536340381783	Illumina PCR Primer Index 5 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	1053	0.10739944433791761	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGTA	20	7.0327776E-4	45.000004	37
TATATCG	20	7.0327776E-4	45.000004	43
CACGATA	20	7.0327776E-4	45.000004	18
CTAAGCG	20	7.0327776E-4	45.000004	43
CACGAAC	20	7.0327776E-4	45.000004	42
TCGATCG	20	7.0327776E-4	45.000004	2
ATCGTAA	20	7.0327776E-4	45.000004	21
CGTTAGT	20	7.0327776E-4	45.000004	12
CGCCTAC	20	7.0327776E-4	45.000004	28
AGACGAC	20	7.0327776E-4	45.000004	28
ATATCGT	20	7.0327776E-4	45.000004	44
ACTAGCG	20	7.0327776E-4	45.000004	35
AACATCG	20	7.0327776E-4	45.000004	29
CGCTAAC	20	7.0327776E-4	45.000004	25
AACGCCC	20	7.0327776E-4	45.000004	10
TCGTATC	20	7.0327776E-4	45.000004	29
CGTGTTA	20	7.0327776E-4	45.000004	32
CATCGCA	20	7.0327776E-4	45.000004	22
ACCGTCG	20	7.0327776E-4	45.000004	35
TTCGCTA	20	7.0327776E-4	45.000004	23