##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935285.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	980452
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28754288838209	33.0	31.0	34.0	30.0	34.0
2	32.442701937473736	34.0	31.0	34.0	30.0	34.0
3	32.50170125615532	34.0	31.0	34.0	30.0	34.0
4	35.957662384288064	37.0	35.0	37.0	35.0	37.0
5	31.901016061979576	37.0	35.0	37.0	0.0	37.0
6	33.52453664228336	37.0	35.0	37.0	17.0	37.0
7	35.254581560341556	37.0	35.0	37.0	32.0	37.0
8	35.77053746639305	37.0	35.0	37.0	35.0	37.0
9	37.818846817590256	39.0	38.0	39.0	35.0	39.0
10	37.53097652919266	39.0	37.0	39.0	35.0	39.0
11	37.41165911232778	39.0	37.0	39.0	35.0	39.0
12	37.114917405441574	39.0	37.0	39.0	34.0	39.0
13	36.95858134819451	39.0	37.0	39.0	33.0	39.0
14	38.061717452766686	40.0	37.0	41.0	33.0	41.0
15	38.19257954494458	40.0	37.0	41.0	33.0	41.0
16	38.300340047243516	40.0	37.0	41.0	34.0	41.0
17	38.30124983171027	40.0	37.0	41.0	34.0	41.0
18	38.29953429642654	40.0	37.0	41.0	34.0	41.0
19	38.32795486163525	40.0	37.0	41.0	34.0	41.0
20	38.2381687221812	40.0	37.0	41.0	34.0	41.0
21	38.17257244617789	40.0	37.0	41.0	34.0	41.0
22	38.16036277145643	40.0	36.0	41.0	34.0	41.0
23	38.11230738475724	40.0	36.0	41.0	34.0	41.0
24	38.08336257154863	40.0	36.0	41.0	34.0	41.0
25	37.996803515113434	40.0	36.0	41.0	34.0	41.0
26	37.94806986981515	40.0	36.0	41.0	34.0	41.0
27	37.84311623618494	40.0	36.0	41.0	34.0	41.0
28	37.84847192927344	40.0	36.0	41.0	34.0	41.0
29	37.82315401467894	40.0	36.0	41.0	34.0	41.0
30	37.78101018713818	40.0	36.0	41.0	34.0	41.0
31	37.58715877982808	40.0	35.0	41.0	33.0	41.0
32	37.61959483993097	40.0	35.0	41.0	33.0	41.0
33	37.61332324274926	40.0	35.0	41.0	33.0	41.0
34	37.60844182071126	40.0	35.0	41.0	33.0	41.0
35	37.531932210857846	40.0	35.0	41.0	33.0	41.0
36	37.48845634462472	39.0	35.0	41.0	33.0	41.0
37	37.43285137875184	39.0	35.0	41.0	33.0	41.0
38	37.44166262091362	39.0	35.0	41.0	33.0	41.0
39	37.47779187558391	39.0	35.0	41.0	33.0	41.0
40	37.41856103103466	39.0	35.0	41.0	33.0	41.0
41	37.38640341393561	39.0	35.0	41.0	33.0	41.0
42	37.36416979107595	39.0	35.0	41.0	33.0	41.0
43	37.29351258399187	39.0	35.0	41.0	33.0	41.0
44	37.21951712067495	39.0	35.0	41.0	33.0	41.0
45	37.18011896553834	39.0	35.0	41.0	33.0	41.0
46	37.06940880328665	39.0	35.0	41.0	32.0	41.0
47	37.018172230767036	39.0	35.0	41.0	32.0	41.0
48	37.004877342287024	39.0	35.0	41.0	32.0	41.0
49	37.01017591886191	39.0	35.0	41.0	32.0	41.0
50	36.94552512514636	39.0	35.0	41.0	32.0	41.0
51	36.30108256192042	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	10.0
11	10.0
12	11.0
13	10.0
14	13.0
15	21.0
16	33.0
17	67.0
18	112.0
19	173.0
20	315.0
21	646.0
22	1021.0
23	1504.0
24	2175.0
25	3262.0
26	4400.0
27	5440.0
28	7234.0
29	9193.0
30	12321.0
31	16760.0
32	23296.0
33	36650.0
34	63836.0
35	95567.0
36	77788.0
37	121605.0
38	198835.0
39	297746.0
40	391.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.209193310840305	4.7209858310248745	54.44019697037693	17.62962388775789
2	19.668887411112426	5.405874025449487	52.58625613492553	22.338982428512562
3	19.969361070200275	5.173124232496849	51.43321651646383	23.424298180839042
4	19.19461636061735	4.790953560194685	51.13804653363959	24.876383545548382
5	16.52615324360601	16.29697323275387	44.01520931162362	23.161664212016497
6	20.768176310518005	7.027473043045453	53.49471468261577	18.709635963820766
7	81.54024878321428	2.3943038516928925	10.441918625287112	5.623528739805722
8	82.63250011219316	3.1531375324850166	7.8762652327701925	6.338097122551639
9	77.61532436060102	4.719251936861775	10.472822738900017	7.19260096363718
10	40.061828625980674	25.90305287765235	17.6909221461122	16.34419635025478
11	30.57895746043662	24.487889259239616	24.81722715645437	20.1159261238694
12	27.935686805677378	20.29451722266873	31.375732825268344	20.394063146385545
13	23.430213819748445	26.761738463484193	30.152011521216743	19.656036195550623
14	17.954678046452045	29.488848000718036	30.368136328958478	22.18833762387144
15	15.588116501368757	24.46891841721981	38.377809418513095	21.565155662898334
16	17.896847576423937	23.706616948101487	34.090909090909086	24.30562638456549
17	17.533341764818676	23.211947142746407	29.794217360972286	29.460493731462634
18	19.101394050907132	22.99908613578227	32.42484078771832	25.474679025592277
19	21.65929591657725	25.776070628648828	29.49313173923864	23.071501715535284
20	25.43306556567787	24.609363844430938	29.60073517112515	20.35683541876604
21	22.457805175572084	27.834203000248863	29.068633650601967	20.639358173577087
22	21.04488542019395	25.141363371179825	27.251716555221467	26.562034653404755
23	19.75476616907304	26.95471068445982	26.962972180178124	26.327550966289014
24	21.847474430160783	23.34994471937433	30.633320142138526	24.169260708326366
25	19.82391794804845	25.576570806117992	28.145997968284014	26.45351327754954
26	20.467090688784356	27.507720928714512	27.470391207320706	24.554797175180425
27	19.94070081962197	26.742767621464385	30.159967035612144	23.156564523301498
28	17.633499651181292	23.61431258235997	35.264755439327985	23.487432327130755
29	19.715702553516135	22.32674317559656	33.36930313773647	24.58825113315083
30	22.61558954441421	24.034730920024643	30.96459592106498	22.38508361449617
31	22.92626258093206	25.805036860550036	27.55994174115612	23.70875881736179
32	22.951149061861265	25.46305173532208	28.615271323838392	22.970527878978267
33	22.989906696095268	23.54730267264486	27.93048512318808	25.53230550807179
34	19.98945384373738	22.917797097665158	29.774532562532386	27.31821649606508
35	20.156519646040806	22.420781435501176	30.435962188867993	26.986736729590028
36	23.971494779958633	22.415783740560478	30.017787714237926	23.59493376524297
37	19.08578900343923	24.601000354938336	33.96719064268317	22.346019998939266
38	19.58596647260651	25.027232337738102	31.51597426493087	23.870826924724515
39	21.605545197521142	22.613855650251107	31.02099847825289	24.75960067397486
40	23.97537054338203	21.70968084108146	29.092500193788172	25.22244842174834
41	18.275958435497095	21.798823399819675	29.961181169501415	29.964036995181814
42	20.771644098844206	20.796020610901913	30.031964848865627	28.400370441388255
43	21.681326571826055	21.20297577035898	30.407301938289688	26.708395719525278
44	20.37142052849094	22.656693035457113	30.329582682273077	26.64230375377887
45	21.600649496354745	22.395282991926173	28.42403299702586	27.580034514693224
46	22.97185379804417	22.152333821543532	30.484613219209102	24.3911991612032
47	18.827336779362987	22.294819124240657	33.518214048214496	25.35963004818186
48	19.549146720084206	20.86201058287402	33.82470533998605	25.764137357055727
49	20.849465348635118	18.804796155242684	34.56558811650137	25.780150379620824
50	20.584893498100875	20.598866645179978	30.97040956620008	27.845830290519068
51	18.9075038859628	21.11679103107546	28.6374039728615	31.338301110100243
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	353.0
1	528.0
2	703.0
3	1112.0
4	1521.0
5	1200.0
6	879.0
7	930.5
8	982.0
9	1071.5
10	1161.0
11	1293.5
12	1426.0
13	1576.0
14	1726.0
15	1586.0
16	1446.0
17	1439.5
18	1433.0
19	1939.0
20	2445.0
21	2280.0
22	2115.0
23	2588.0
24	3061.0
25	3685.0
26	5594.5
27	6880.0
28	7431.5
29	7983.0
30	10340.0
31	12697.0
32	15547.0
33	18397.0
34	21027.5
35	23658.0
36	25780.0
37	27902.0
38	31920.5
39	35939.0
40	41331.0
41	46723.0
42	54072.5
43	61422.0
44	68850.0
45	76278.0
46	92929.5
47	109581.0
48	119976.0
49	130371.0
50	122253.5
51	114136.0
52	95439.0
53	76742.0
54	63982.5
55	51223.0
56	45304.0
57	39385.0
58	35357.0
59	31329.0
60	28091.5
61	24854.0
62	22479.0
63	20104.0
64	17479.5
65	14855.0
66	12527.0
67	10199.0
68	8462.0
69	6725.0
70	5435.5
71	4146.0
72	3202.5
73	2259.0
74	1922.0
75	1137.0
76	689.0
77	481.0
78	273.0
79	287.0
80	301.0
81	247.5
82	194.0
83	125.0
84	56.0
85	30.0
86	4.0
87	2.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	980452.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.871831752032175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.76193625799539	15.459860462866981
2	12.927682453163527	6.1721492093123675
3	6.191040941838203	4.433744632005133
4	3.4310127332261278	3.276182348266168
5	2.218809113851929	2.648351892787443
6	1.5467673802691462	2.2154502396789977
7	1.1378035222218987	1.9013017974545539
8	0.8932019356656231	1.705789306303938
9	0.6872344379613261	1.4764990389493655
>10	4.510789990087449	22.136117459905243
>50	1.3933547487956635	23.753082985598496
>100	0.28740102514275545	10.207512702487241
>500	0.006914911883133964	1.1763415825282195
>1k	0.0051861839123504725	2.1950397745895804
>5k	8.643639853917455E-4	1.242576567266258
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	6640	0.6772386613521111	Illumina PCR Primer Index 5 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGCT	5404	0.5511743563172904	Illumina PCR Primer Index 5 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	4503	0.4592779656729753	Illumina PCR Primer Index 5 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	3446	0.3514705462378576	Illumina PCR Primer Index 5 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTG	1712	0.17461334160162864	Illumina PCR Primer Index 5 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGC	1683	0.17165552214692814	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCG	1599	0.16308804510572675	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTG	1481	0.1510527797383248	Illumina PCR Primer Index 5 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTC	1436	0.14646305989482403	No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1119	0.11413103344171872	No Hit
TGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTG	1097	0.11188717040711835	Illumina PCR Primer Index 5 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTG	1081	0.11025527001831809	Illumina PCR Primer Index 5 (95% over 22bp)
TTCCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCT	1066	0.10872536340381783	Illumina PCR Primer Index 5 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	1053	0.10739944433791761	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.0797509720006693E-4	0.0	0.0	0.5697372232398934	0.0
2	4.0797509720006693E-4	0.0	0.0	2.1568623451224536	0.0
3	4.0797509720006693E-4	0.0	0.0	3.0536936025425008	0.0
4	5.099688715000837E-4	0.0	0.0	4.044257138544263	0.0
5	5.099688715000837E-4	0.0	0.0	5.714915161578538	0.0
6	5.099688715000837E-4	0.0	0.0	6.7104763925210005	0.0
7	5.099688715000837E-4	0.0	0.0	7.635355937873552	0.0
8	5.099688715000837E-4	0.0	0.0	9.1457817414825	0.0
9	6.119626458001003E-4	0.0	0.0	9.850456728121316	0.0
10	6.119626458001003E-4	0.0	0.0	11.292138727852052	0.0
11	6.119626458001003E-4	0.0	0.0	13.826480031658868	0.0
12	6.119626458001003E-4	0.0	0.0	16.233329117590664	0.0
13	6.119626458001003E-4	0.0	0.0	16.932292452868676	0.0
14	6.119626458001003E-4	0.0	0.0	17.180749287063517	0.0
15	6.119626458001003E-4	0.0	0.0	17.589642328232284	0.0
16	6.119626458001003E-4	0.0	0.0	18.493715143627632	0.0
17	6.119626458001003E-4	0.0	0.0	19.768739316152143	0.0
18	6.119626458001003E-4	0.0	0.0	21.290588422482692	0.0
19	6.119626458001003E-4	0.0	0.0	22.234540803629347	0.0
20	6.119626458001003E-4	0.0	0.0	23.1722715645437	0.0
21	6.119626458001003E-4	0.0	0.0	24.422511249913306	0.0
22	6.119626458001003E-4	0.0	0.0	25.634503269920405	0.0
23	7.139564201001171E-4	0.0	0.0	26.95205884632802	0.0
24	7.139564201001171E-4	0.0	0.0	27.895705246151774	0.0
25	8.159501944001339E-4	0.0	0.0	28.684015127716606	0.0
26	8.159501944001339E-4	0.0	0.0	29.45366014858453	0.0
27	8.159501944001339E-4	0.0	0.0	30.095506970254537	0.0
28	8.159501944001339E-4	0.0	0.0	30.772133668960848	0.0
29	8.159501944001339E-4	0.0	0.0	31.545858440800775	0.0
30	8.159501944001339E-4	0.0	0.0	32.40128022585501	0.0
31	8.159501944001339E-4	0.0	0.0	33.17633091676084	0.0
32	0.0010199377430001673	0.0	0.0	33.84418615087735	0.0
33	0.0010199377430001673	0.0	0.0	34.555082757748465	0.0
34	0.0010199377430001673	0.0	0.0	35.23548322610388	0.0
35	0.0010199377430001673	0.0	0.0	36.02134525708551	0.0
36	0.0010199377430001673	0.0	0.0	36.72000261104062	0.0
37	0.001121931517300184	0.0	0.0	37.41121441947183	0.0
38	0.001121931517300184	0.0	0.0	38.101712271482945	0.0
39	0.001121931517300184	0.0	0.0	38.84300302309547	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGTA	20	7.0327776E-4	45.000004	37
TATATCG	20	7.0327776E-4	45.000004	43
CACGATA	20	7.0327776E-4	45.000004	18
CTAAGCG	20	7.0327776E-4	45.000004	43
CACGAAC	20	7.0327776E-4	45.000004	42
TCGATCG	20	7.0327776E-4	45.000004	2
ATCGTAA	20	7.0327776E-4	45.000004	21
CGTTAGT	20	7.0327776E-4	45.000004	12
CGCCTAC	20	7.0327776E-4	45.000004	28
AGACGAC	20	7.0327776E-4	45.000004	28
ATATCGT	20	7.0327776E-4	45.000004	44
ACTAGCG	20	7.0327776E-4	45.000004	35
AACATCG	20	7.0327776E-4	45.000004	29
CGCTAAC	20	7.0327776E-4	45.000004	25
AACGCCC	20	7.0327776E-4	45.000004	10
TCGTATC	20	7.0327776E-4	45.000004	29
CGTGTTA	20	7.0327776E-4	45.000004	32
CATCGCA	20	7.0327776E-4	45.000004	22
ACCGTCG	20	7.0327776E-4	45.000004	35
TTCGCTA	20	7.0327776E-4	45.000004	23
>>END_MODULE