FastQCFastQC Report
Sat 14 Jan 2017
SRR2935284.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935284.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562941
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC45570.8094986863632245TruSeq Adapter, Index 27 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGCT44990.7991956528304032Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC37060.6583283150454488TruSeq Adapter, Index 27 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC28050.49827601826834433TruSeq Adapter, Index 27 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG15290.2716092805462739No Hit
TTCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCT9570.17000005329155277No Hit
TGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG8890.15792063466686562No Hit
GCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG8610.1529467564096415TruSeq Adapter, Index 27 (95% over 21bp)
TGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGCTT7570.13447235145423764TruSeq Adapter, Index 27 (95% over 24bp)
ATTTTTGGGCCGATCCTTTTGGGAACATCTCCTGGAAAAACCTTTGCTGGA6910.12274820984792367No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTC6540.11617558500802037No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCC6220.11049115271404995No Hit
TTCTGGGGGGAAAAACCTTTGCTGGATATTTGTTAAATATCAATAGCCCTA6050.10747129805787818No Hit
TTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG6050.10747129805787818No Hit
CGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG6020.10693838253031844No Hit
CTACACAAAAATAAATAAAGGTAAAAACTCTCATACGCGCATGTCATCGGG5900.10480672042007956No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTCG253.8882597E-545.00000417
GGTACTA253.8882597E-545.0000048
CACGGGA253.8882597E-545.0000044
CAACGAA253.8882597E-545.00000412
GCTATCG253.8882597E-545.00000425
TCTTACG253.8882597E-545.0000041
GGTCCAC253.8882597E-545.0000048
ATAGCAG253.8882597E-545.00000415
GCACGGG351.2104465E-745.0000043
TTGTACG700.045.0000041
CAATTAG253.8882597E-545.00000427
CGTTATT351.2104465E-745.0000041
CATTAGG253.8882597E-545.00000417
GGCCAGA253.8882597E-545.0000048
TGCGCAA253.8882597E-545.0000041
GGGCGTA253.8882597E-545.0000047
TACGAAA253.8882597E-545.00000420
TCGCTAT253.8882597E-545.00000423
GCCCTAC1000.045.00000426
TTGCGAT253.8882597E-545.00000417
Produced by FastQC (version 0.11.5)