##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935284.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	562941
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24781637862582	33.0	31.0	34.0	30.0	34.0
2	32.398327711074515	34.0	31.0	34.0	30.0	34.0
3	32.45748488740383	34.0	31.0	34.0	30.0	34.0
4	35.933136865142174	37.0	35.0	37.0	35.0	37.0
5	31.7276108863984	37.0	35.0	37.0	0.0	37.0
6	33.45010223096204	37.0	35.0	37.0	17.0	37.0
7	35.216294425170666	37.0	35.0	37.0	32.0	37.0
8	35.75116930548672	37.0	35.0	37.0	35.0	37.0
9	37.7588610529345	39.0	38.0	39.0	35.0	39.0
10	37.498096603374066	39.0	37.0	39.0	35.0	39.0
11	37.395787480393146	39.0	37.0	39.0	35.0	39.0
12	36.983140329093104	39.0	35.0	39.0	34.0	39.0
13	36.75017630622037	39.0	35.0	39.0	33.0	39.0
14	37.80257789004531	40.0	36.0	41.0	33.0	41.0
15	37.98172810294507	40.0	36.0	41.0	33.0	41.0
16	38.10861884282723	40.0	36.0	41.0	34.0	41.0
17	38.1295659758305	40.0	36.0	41.0	34.0	41.0
18	38.134502550000796	40.0	36.0	41.0	34.0	41.0
19	38.158901199237576	40.0	36.0	41.0	34.0	41.0
20	38.06440284150559	40.0	36.0	41.0	34.0	41.0
21	37.99931253896945	40.0	36.0	41.0	34.0	41.0
22	37.952666442842144	40.0	36.0	41.0	34.0	41.0
23	37.929289215033194	40.0	35.0	41.0	34.0	41.0
24	37.887942075634925	39.0	35.0	41.0	34.0	41.0
25	37.81113118426265	39.0	35.0	41.0	34.0	41.0
26	37.77504747389158	39.0	35.0	41.0	34.0	41.0
27	37.72268141776847	39.0	35.0	41.0	34.0	41.0
28	37.742672855592325	39.0	35.0	41.0	34.0	41.0
29	37.6694982245031	39.0	35.0	41.0	34.0	41.0
30	37.63294910123796	39.0	36.0	41.0	34.0	41.0
31	37.427899193698806	39.0	35.0	41.0	33.0	41.0
32	37.431089581323796	39.0	35.0	41.0	33.0	41.0
33	37.45828603708026	39.0	35.0	41.0	33.0	41.0
34	37.443275583053996	39.0	35.0	41.0	33.0	41.0
35	37.35173135372979	39.0	35.0	41.0	33.0	41.0
36	37.37513700370021	39.0	35.0	41.0	33.0	41.0
37	37.292289955785776	39.0	35.0	41.0	33.0	41.0
38	37.24106433889164	39.0	35.0	41.0	33.0	41.0
39	37.254417425627196	39.0	35.0	41.0	33.0	41.0
40	37.221644897067364	39.0	35.0	41.0	33.0	41.0
41	37.21391229276247	39.0	35.0	41.0	33.0	41.0
42	37.216877079480795	39.0	35.0	41.0	33.0	41.0
43	37.14198468400774	39.0	35.0	41.0	33.0	41.0
44	37.055034186531095	39.0	35.0	41.0	33.0	41.0
45	37.00765444336085	39.0	35.0	41.0	33.0	41.0
46	36.92853602775424	39.0	35.0	40.0	32.0	41.0
47	36.87374698236582	39.0	35.0	40.0	32.0	41.0
48	36.89291773027724	39.0	35.0	40.0	33.0	41.0
49	36.9284134571829	39.0	35.0	40.0	33.0	41.0
50	36.84416661781608	38.0	35.0	40.0	32.0	41.0
51	36.164940908549916	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	6.0
11	5.0
12	6.0
13	5.0
14	8.0
15	11.0
16	26.0
17	40.0
18	58.0
19	90.0
20	176.0
21	275.0
22	456.0
23	771.0
24	1143.0
25	1772.0
26	2408.0
27	3054.0
28	4075.0
29	5455.0
30	7195.0
31	10039.0
32	14214.0
33	22926.0
34	41142.0
35	64110.0
36	46448.0
37	69901.0
38	113163.0
39	153802.0
40	157.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.31832465569216	5.00212278018478	55.787196171534845	16.892356392588212
2	19.861761712151008	5.233763396164074	52.62718473161486	22.27729016007006
3	19.87668334692268	4.795351555491606	53.031845255541874	22.296119842043836
4	19.570256918575836	4.896072590200394	50.42428957919214	25.109380912031632
5	15.870757326256216	17.198072266898308	44.76721361563645	22.163956791209024
6	20.497707575038948	6.542071016323203	53.57328743154256	19.386933977095293
7	80.41908477087297	2.56563298818171	10.756544646774707	6.258737594170614
8	80.23061031262601	3.3216624832797756	9.054767728767313	7.392959475326899
9	74.29499716666578	6.051611092459068	12.392595316383066	7.260796424492087
10	39.95356529369863	25.751899399759477	18.922764552590767	15.371770753951125
11	32.82617538960566	23.53834593678556	24.78678937934881	18.84868929425997
12	29.194533707795312	20.32433239007285	31.256028606905517	19.22510529522632
13	21.622692253717528	29.679486837874663	30.85563140719898	17.84218950120883
14	16.18677623409913	31.988076903263398	31.38765874221277	20.4374881204247
15	13.142585102168788	24.774709960724124	41.60631398317053	20.47639095393656
16	15.458103069415799	22.926025995619433	37.10122375168979	24.514647183274977
17	15.72988998847126	23.15606786501605	29.782872450221248	31.33116969629144
18	17.94610802908298	23.310968645026744	34.323668022048494	24.419255303841787
19	21.372577232782834	23.835357524145515	30.670354442117382	24.121710800954276
20	24.990007833858254	24.66759393968462	30.430364816206318	19.912033410250807
21	22.549432356143896	27.714272010743578	28.987407206083766	20.748888427028763
22	21.13134413730746	24.31231692131147	27.493644982333848	27.06269395904722
23	19.58624438440263	26.608294652547958	27.173718027288828	26.631742935760588
24	21.28873185644677	23.106861997971368	31.501169749582992	24.10323639599887
25	17.53185502565988	26.232766844127536	29.872935174378846	26.362442955833735
26	16.59516716671907	27.983571990670423	29.351743788425427	26.069517054185077
27	18.714927496842478	27.77022814113735	29.7027574825781	23.812086879442074
28	17.47181320955482	24.413748510057005	33.63922684615262	24.475211434235558
29	17.02096667323929	24.008022155074865	33.54259149715512	25.428419674530723
30	18.900559738942448	26.478796179350944	31.265621086401595	23.355022995305013
31	23.586663611284308	26.3629758713613	27.712850902670084	22.337509614684308
32	23.731083719252993	27.400384765010898	26.14021718084133	22.728314334894776
33	22.052577445949044	24.163278212103933	27.442307453178934	26.341836888768093
34	19.55853277696952	23.96574418988846	30.878546774884047	25.59717625825797
35	18.628950458396172	22.49827246549816	31.14589273120984	27.726884344895826
36	23.32091640154119	21.619494760552172	29.595641461538598	25.46394737636804
37	21.00699718087686	25.514929628504586	30.780490317813058	22.697582872805498
38	20.566808955112524	27.938096532318664	27.179047182564425	24.316047330004388
39	20.011333336886103	25.892944376053617	31.158149788343714	22.937572498716563
40	22.710728122485303	21.921835503187722	31.860710092176625	23.50672628215035
41	19.36632791002965	21.559275305937923	29.39064662193729	29.68375016209514
42	22.173904547723474	20.93381011509199	27.265024221010727	29.62726111617381
43	22.136067545266734	21.98880522115106	27.373738988632912	28.501388244949293
44	20.39432196269236	22.83081175469543	29.38638329771681	27.388482984895397
45	19.512169126071825	25.175462437449042	28.418253422649975	26.894115013829158
46	23.347739816428366	22.414782366180468	31.264910532364848	22.97256728502632
47	18.359650478469327	22.350654864364117	33.956844500578214	25.332850156588343
48	19.200768819467758	20.788857091595744	32.78656200205705	27.22381208687944
49	21.033642957254845	18.50034017774509	34.33308286303538	26.13293400196468
50	21.322305534683032	21.045900014388717	30.86184875502051	26.769945695907744
51	18.76662030301577	20.803245810839858	27.82813829513217	32.6019955910122
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	109.0
1	245.0
2	381.0
3	583.5
4	786.0
5	644.0
6	502.0
7	589.5
8	677.0
9	906.5
10	1136.0
11	1245.0
12	1354.0
13	1479.5
14	1605.0
15	1520.5
16	1436.0
17	1298.0
18	1160.0
19	1414.5
20	1669.0
21	1671.0
22	1673.0
23	1667.0
24	1661.0
25	1842.0
26	2865.5
27	3708.0
28	3843.0
29	3978.0
30	5733.5
31	7489.0
32	8273.0
33	9057.0
34	9806.0
35	10555.0
36	12839.5
37	15124.0
38	16908.0
39	18692.0
40	24235.5
41	29779.0
42	35221.5
43	40664.0
44	47148.0
45	53632.0
46	61000.0
47	68368.0
48	74601.0
49	80834.0
50	74504.0
51	68174.0
52	53465.5
53	38757.0
54	32076.0
55	25395.0
56	21864.0
57	18333.0
58	16105.0
59	13877.0
60	13611.0
61	13345.0
62	11612.0
63	9879.0
64	8368.5
65	6858.0
66	5427.5
67	3997.0
68	2991.5
69	1986.0
70	1860.5
71	1735.0
72	1417.5
73	1100.0
74	902.5
75	536.5
76	368.0
77	304.0
78	240.0
79	155.0
80	70.0
81	54.5
82	39.0
83	24.0
84	9.0
85	9.0
86	9.0
87	5.0
88	1.0
89	6.0
90	11.0
91	6.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	562941.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.65180136869179
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.77183105890705	16.615141446095453
2	13.085035511734237	6.713094636985699
3	6.088377876569289	4.6853357984187864
4	3.6387587995965025	3.7336287182331542
5	2.2831372360909334	2.9283291438834302
6	1.5534276147007453	2.3908929967766523
7	1.17695064447735	2.113363290702074
8	0.9116887252953216	1.8709166473081125
9	0.663018605280797	1.5306859409769067
>10	4.47524585195141	24.16668370267309
>50	1.156630326417532	20.3908020356573
>100	0.1826989644731597	8.144631149811259
>500	0.009725420162069337	1.6700538908684004
>1k	0.0034733643435961923	3.046440601609707
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	4557	0.8094986863632245	TruSeq Adapter, Index 27 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGCT	4499	0.7991956528304032	Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	3706	0.6583283150454488	TruSeq Adapter, Index 27 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	2805	0.49827601826834433	TruSeq Adapter, Index 27 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	1529	0.2716092805462739	No Hit
TTCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCT	957	0.17000005329155277	No Hit
TGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	889	0.15792063466686562	No Hit
GCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	861	0.1529467564096415	TruSeq Adapter, Index 27 (95% over 21bp)
TGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGCTT	757	0.13447235145423764	TruSeq Adapter, Index 27 (95% over 24bp)
ATTTTTGGGCCGATCCTTTTGGGAACATCTCCTGGAAAAACCTTTGCTGGA	691	0.12274820984792367	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTC	654	0.11617558500802037	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCC	622	0.11049115271404995	No Hit
TTCTGGGGGGAAAAACCTTTGCTGGATATTTGTTAAATATCAATAGCCCTA	605	0.10747129805787818	No Hit
TTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	605	0.10747129805787818	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	602	0.10693838253031844	No Hit
CTACACAAAAATAAATAAAGGTAAAAACTCTCATACGCGCATGTCATCGGG	590	0.10480672042007956	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8182029733133668	0.0
2	0.0	0.0	0.0	2.911139888549599	0.0
3	0.0	0.0	0.0	4.0707640765195645	0.0
4	0.0	0.0	0.0	5.3373266470198475	0.0
5	0.0	0.0	0.0	7.452113098886029	0.0
6	0.0	0.0	0.0	8.741413398562194	0.0
7	0.0	0.0	0.0	9.903346887151585	0.0
8	0.0	0.0	0.0	11.83321165095454	0.0
9	0.0	0.0	0.0	12.714476295029142	0.0
10	0.0	0.0	0.0	14.443076627923707	0.0
11	0.0	0.0	0.0	17.426515389712243	0.0
12	1.776385091865755E-4	0.0	0.0	20.127686560403312	0.0
13	1.776385091865755E-4	0.0	0.0	20.95157396601065	0.0
14	1.776385091865755E-4	0.0	0.0	21.214656598115965	0.0
15	1.776385091865755E-4	0.0	0.0	21.663904387848817	0.0
16	1.776385091865755E-4	0.0	0.0	22.759578712511612	0.0
17	1.776385091865755E-4	0.0	0.0	24.462421461574127	0.0
18	1.776385091865755E-4	0.0	0.0	26.453926788064823	0.0
19	1.776385091865755E-4	0.0	0.0	27.539653356213172	0.0
20	1.776385091865755E-4	0.0	0.0	28.702119760330124	0.0
21	1.776385091865755E-4	0.0	0.0	30.238692864794	0.0
22	1.776385091865755E-4	0.0	0.0	31.577376670024034	0.0
23	1.776385091865755E-4	0.0	0.0	32.868098077773695	0.0
24	1.776385091865755E-4	0.0	0.0	33.831786990110864	0.0
25	1.776385091865755E-4	0.0	0.0	34.68587294227992	0.0
26	1.776385091865755E-4	0.0	0.0	35.39465059393436	0.0
27	1.776385091865755E-4	0.0	0.0	36.09685562074889	0.0
28	1.776385091865755E-4	0.0	0.0	36.81114006618811	0.0
29	1.776385091865755E-4	0.0	0.0	37.59417061468253	0.0
30	1.776385091865755E-4	0.0	0.0	38.55057634814305	0.0
31	1.776385091865755E-4	0.0	0.0	39.34550867675298	0.0
32	1.776385091865755E-4	0.0	0.0	40.071694902307705	0.0
33	1.776385091865755E-4	0.0	0.0	40.79006503345821	0.0
34	1.776385091865755E-4	0.0	0.0	41.55248951488699	0.0
35	1.776385091865755E-4	0.0	0.0	42.37388998136572	0.0
36	3.55277018373151E-4	0.0	0.0	43.109135770888955	0.0
37	3.55277018373151E-4	0.0	0.0	43.83976295917334	0.0
38	3.55277018373151E-4	0.0	0.0	44.4966701661453	0.0
39	3.55277018373151E-4	0.0	0.0	45.22694207741131	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACTCG	25	3.8882597E-5	45.000004	17
GGTACTA	25	3.8882597E-5	45.000004	8
CACGGGA	25	3.8882597E-5	45.000004	4
CAACGAA	25	3.8882597E-5	45.000004	12
GCTATCG	25	3.8882597E-5	45.000004	25
TCTTACG	25	3.8882597E-5	45.000004	1
GGTCCAC	25	3.8882597E-5	45.000004	8
ATAGCAG	25	3.8882597E-5	45.000004	15
GCACGGG	35	1.2104465E-7	45.000004	3
TTGTACG	70	0.0	45.000004	1
CAATTAG	25	3.8882597E-5	45.000004	27
CGTTATT	35	1.2104465E-7	45.000004	1
CATTAGG	25	3.8882597E-5	45.000004	17
GGCCAGA	25	3.8882597E-5	45.000004	8
TGCGCAA	25	3.8882597E-5	45.000004	1
GGGCGTA	25	3.8882597E-5	45.000004	7
TACGAAA	25	3.8882597E-5	45.000004	20
TCGCTAT	25	3.8882597E-5	45.000004	23
GCCCTAC	100	0.0	45.000004	26
TTGCGAT	25	3.8882597E-5	45.000004	17
>>END_MODULE