FastQCFastQC Report
Sat 14 Jan 2017
SRR2935283.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935283.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584073
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT47990.8216438698587334RNA PCR Primer, Index 16 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC46360.7937363993884327RNA PCR Primer, Index 16 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC40480.6930640519250162RNA PCR Primer, Index 14 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC35250.6035204503546646RNA PCR Primer, Index 14 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG15160.25955659652132523RNA PCR Primer, Index 16 (95% over 22bp)
TTCCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT10100.1729235900306982RNA PCR Primer, Index 14 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG10090.1727523785554203RNA PCR Primer, Index 14 (95% over 22bp)
TGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCTT8260.14142067857956112RNA PCR Primer, Index 14 (96% over 25bp)
TGCTTTGGGATCGTTCTCTTAAAGTGCCAGTGTTTTAAATAGCGTTCTTGT7990.1367979687470573No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA7770.13303131629094309No Hit
TGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG7620.1304631441617743RNA PCR Primer, Index 14 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCC7490.12823739498316136No Hit
TGTTTTGGGATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAA7040.120532878595655No Hit
TTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG6850.11727986056537454RNA PCR Primer, Index 14 (95% over 22bp)
TTTCCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC6480.11094503598009153No Hit
CGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG6070.10392536549369685RNA PCR Primer, Index 16 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC5980.10238446221619557No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA302.1636606E-645.00000419
CCGGGTT302.1636606E-645.0000045
GAGCTTA302.1636606E-645.0000049
TCGGGTA600.045.0000045
TACGCGC302.1636606E-645.00000434
GTAGATT302.1636606E-645.0000049
TGCGAAT302.1636606E-645.00000427
ACGGACG302.1636606E-645.00000411
GTCAAGG302.1636606E-645.00000426
ATGCGTC302.1636606E-645.00000418
ATGCGAA302.1636606E-645.00000426
AAGGACG302.1636606E-645.00000439
TAATGCG302.1636606E-645.00000424
CGGACGG302.1636606E-645.00000412
TATTTCG207.0304336E-445.01
AGCGTGT207.0304336E-445.016
GCCCATA207.0304336E-445.023
GTCGCAA207.0304336E-445.040
ACACGTA351.2105556E-745.012
TCGCAAT207.0304336E-445.041
Produced by FastQC (version 0.11.5)