##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935283.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	584073
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.238358903767164	33.0	31.0	34.0	30.0	34.0
2	32.381632090509235	34.0	31.0	34.0	30.0	34.0
3	32.44502998768989	34.0	31.0	34.0	30.0	34.0
4	35.92170499235541	37.0	35.0	37.0	35.0	37.0
5	31.776004026893897	37.0	35.0	37.0	0.0	37.0
6	33.49405810575048	37.0	35.0	37.0	17.0	37.0
7	35.22409185153226	37.0	35.0	37.0	32.0	37.0
8	35.763459362100285	37.0	35.0	37.0	35.0	37.0
9	37.78313498483922	39.0	38.0	39.0	35.0	39.0
10	37.521080412893596	39.0	37.0	39.0	35.0	39.0
11	37.38584560491582	39.0	37.0	39.0	35.0	39.0
12	37.02957335812476	39.0	35.0	39.0	34.0	39.0
13	36.84131778048292	39.0	35.0	39.0	33.0	39.0
14	37.889405947544226	40.0	36.0	41.0	33.0	41.0
15	38.07252689304248	40.0	36.0	41.0	33.0	41.0
16	38.19986371566568	40.0	36.0	41.0	34.0	41.0
17	38.24035351745415	40.0	36.0	41.0	34.0	41.0
18	38.25567865660628	40.0	36.0	41.0	34.0	41.0
19	38.28589234564858	40.0	37.0	41.0	34.0	41.0
20	38.17558764058602	40.0	36.0	41.0	34.0	41.0
21	38.10194444872473	40.0	36.0	41.0	34.0	41.0
22	38.06934920806132	40.0	36.0	41.0	34.0	41.0
23	38.04787415271721	40.0	36.0	41.0	34.0	41.0
24	38.01685063339685	40.0	36.0	41.0	34.0	41.0
25	37.92179059809305	40.0	36.0	41.0	34.0	41.0
26	37.8609557366973	40.0	36.0	41.0	34.0	41.0
27	37.78867710029397	40.0	35.0	41.0	34.0	41.0
28	37.7834911047078	40.0	36.0	41.0	34.0	41.0
29	37.76943293047273	40.0	36.0	41.0	34.0	41.0
30	37.65929601265595	39.0	36.0	41.0	33.0	41.0
31	37.37695288088989	39.0	35.0	41.0	33.0	41.0
32	37.4259655899177	39.0	35.0	41.0	33.0	41.0
33	37.440263460218155	39.0	35.0	41.0	33.0	41.0
34	37.4031088579681	39.0	35.0	41.0	33.0	41.0
35	37.36530878845624	39.0	35.0	41.0	33.0	41.0
36	37.35688860810207	39.0	35.0	41.0	33.0	41.0
37	37.264609047156775	39.0	35.0	41.0	33.0	41.0
38	37.2802098367841	39.0	35.0	41.0	33.0	41.0
39	37.28935595379345	39.0	35.0	41.0	33.0	41.0
40	37.13689727140272	39.0	35.0	41.0	33.0	41.0
41	37.12198988825027	39.0	35.0	41.0	33.0	41.0
42	37.19403738916197	39.0	35.0	41.0	33.0	41.0
43	37.160755590482694	39.0	35.0	41.0	33.0	41.0
44	37.08999388775033	39.0	35.0	41.0	33.0	41.0
45	37.0825804308708	39.0	35.0	41.0	33.0	41.0
46	36.93308884334664	39.0	35.0	40.0	32.0	41.0
47	36.88928781162629	39.0	35.0	40.0	32.0	41.0
48	36.92330753176401	39.0	35.0	40.0	33.0	41.0
49	36.96117779798073	39.0	35.0	40.0	33.0	41.0
50	36.85647342027452	39.0	35.0	40.0	32.0	41.0
51	36.24117190830598	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	3.0
11	4.0
12	4.0
13	6.0
14	7.0
15	7.0
16	16.0
17	28.0
18	54.0
19	111.0
20	179.0
21	299.0
22	472.0
23	770.0
24	1168.0
25	1792.0
26	2508.0
27	3261.0
28	4151.0
29	5458.0
30	7351.0
31	10341.0
32	14575.0
33	23090.0
34	40454.0
35	61629.0
36	48925.0
37	77496.0
38	125134.0
39	154650.0
40	126.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.366988373028715	3.985289510044121	54.55533811698196	17.092383999945213
2	18.872469708409735	4.787757694671728	52.7630963937727	23.57667620314584
3	20.456347066205765	4.587440268596563	52.41399619568102	22.542216469516653
4	18.148073956508863	5.077105087891411	51.228185517906155	25.546635437693578
5	15.657118202690418	16.53954214627281	44.0648686037533	23.738471047283475
6	20.820856297072453	5.912617087247655	53.829230250328294	19.437296365351592
7	80.65909569522988	2.3664850112913967	10.85052724573812	6.123892047740608
8	80.48240545274307	2.621590109455496	9.084138455295827	7.811865982505611
9	75.10550907164001	5.358063118822476	12.384924487178829	7.151503322358678
10	40.661698109654104	25.601080686831956	18.341714135048186	15.395507068465758
11	30.564672566614103	24.84466838905411	24.872233436573854	19.718425607757933
12	28.618169304179442	20.102624158281586	30.954692307297204	20.324514230241768
13	22.80502608406826	28.019613986607837	30.411267084765086	18.764092844558814
14	16.870665139460307	30.82097614510515	30.38900959297896	21.919349122455582
15	13.442840192921091	25.225271498596925	40.511374434360086	20.8205138741219
16	15.409546409438546	23.314551434495346	37.00992855345137	24.26597360261474
17	15.56397916013923	23.132382424799637	30.26265552422386	31.040982890837277
18	17.555682252047262	22.895939377440833	33.44239504308536	26.105983327426536
19	22.64956606451591	23.87355690127775	30.374456617580336	23.102420416626003
20	24.112910543716282	25.313787831315604	29.78531793114902	20.787983693819097
21	22.860156179107747	26.612084448348067	28.783388377822632	21.74437099472155
22	18.739609603594072	25.657922896624225	27.97355809975808	27.628909400023627
23	18.78737760519661	27.065966069309827	26.792370131815712	27.354286193677847
24	22.335393007380926	23.121424890381853	30.29758266518055	24.24559943705667
25	17.53496566353863	25.580706521273882	29.532096159212973	27.352231655974514
26	16.989999537729016	27.713145445860366	29.95704304085277	25.33981197555785
27	18.522171029991114	28.654979771364196	28.07696983082594	24.74587936781875
28	15.176527591585298	27.328946895336713	32.6551646797575	24.83936083332049
29	16.16972535967251	23.909340099610837	30.73999311729869	29.180941423417963
30	19.75506486346741	25.944017271813625	27.590558029561375	26.710359835157593
31	19.708666553667094	31.357552908626147	22.868031906970533	26.065748630736223
32	20.053315253401543	29.801240598349864	23.527024875315245	26.618419272933348
33	20.028489589486245	27.298984887163076	22.563275480975836	30.109250042374843
34	16.827006213264436	27.287684929794736	27.071273625043446	28.814035231897385
35	15.61328806501927	25.2696152706939	28.323685566701425	30.793411097585405
36	20.288388608958126	24.479474312286307	27.147976365967953	28.084160712787615
37	17.03656905900461	29.888044816315766	27.823748058889898	25.251638065789724
38	16.684044631407374	30.50046826338488	23.51640976384801	29.299077341359727
39	18.387941233373226	26.537436245126894	26.238843432242202	28.83577908925768
40	21.139994486990496	25.391860264042336	27.337678680575888	26.13046656839128
41	16.257043212064247	27.504781080447138	25.44767520498294	30.79050050250568
42	19.02176611485208	25.29101670510364	25.609298837645294	30.07791834239898
43	20.966386051058684	22.39514581225292	27.094387174205963	29.54408096248243
44	18.777104916679935	24.3779459074465	27.215604898702733	29.629344277170834
45	18.486216620182752	24.22488284854804	25.188632242887447	32.10026828838176
46	19.603029073420615	25.211745792049967	28.48924706329517	26.695978071234244
47	14.874681760670327	26.02294576191675	30.988249756451676	28.114122720961248
48	17.172168547424725	23.898040142242493	29.47987665925321	29.449914651079574
49	19.210783583558904	20.86656976097166	31.36234682993393	28.560299825535505
50	18.381435197312666	23.453917575371573	28.05265780133648	30.111989425979285
51	16.86724090995475	23.90642950453111	24.7517005579782	34.47462902753594
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	229.0
1	393.0
2	557.0
3	724.0
4	891.0
5	686.0
6	481.0
7	548.0
8	615.0
9	820.0
10	1025.0
11	1206.0
12	1387.0
13	1321.5
14	1256.0
15	1245.5
16	1235.0
17	1176.5
18	1118.0
19	1087.0
20	1056.0
21	1311.5
22	1567.0
23	1679.0
24	1791.0
25	1913.0
26	2869.5
27	3704.0
28	4310.5
29	4917.0
30	5655.5
31	6394.0
32	8349.0
33	10304.0
34	11089.5
35	11875.0
36	13267.5
37	14660.0
38	16351.0
39	18042.0
40	22813.0
41	27584.0
42	33863.5
43	40143.0
44	45180.5
45	50218.0
46	59655.0
47	69092.0
48	76180.5
49	83269.0
50	77549.5
51	71830.0
52	56791.5
53	41753.0
54	35207.5
55	28662.0
56	24502.5
57	20343.0
58	18785.5
59	17228.0
60	16649.5
61	16071.0
62	14089.5
63	12108.0
64	9333.5
65	6559.0
66	5761.0
67	4963.0
68	4156.0
69	3349.0
70	2734.0
71	2119.0
72	1908.5
73	1698.0
74	1260.0
75	587.5
76	353.0
77	385.0
78	417.0
79	275.0
80	133.0
81	91.5
82	50.0
83	87.0
84	124.0
85	66.5
86	9.0
87	22.5
88	36.0
89	18.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	584073.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.468881561717673
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.89731026300592	17.177592190255485
2	13.17472391325692	6.974404137366539
3	6.176398284763525	4.904470640322057
4	3.517282894417009	3.7239417740551737
5	2.318947373995689	3.068997169507405
6	1.5789136242486825	2.5075246629852463
7	1.147345035869455	2.1258257905397318
8	0.8315328970333056	1.760779661299723
9	0.6961170270113497	1.6582895226952592
>10	4.44718507309598	25.616198363352716
>50	1.069906552659133	18.879829190036077
>100	0.13139203278270054	6.585891306177445
>500	0.008414268109236981	1.4968246278048056
>1k	0.004530759751127604	3.519430963602395
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT	4799	0.8216438698587334	RNA PCR Primer, Index 16 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	4636	0.7937363993884327	RNA PCR Primer, Index 16 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	4048	0.6930640519250162	RNA PCR Primer, Index 14 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	3525	0.6035204503546646	RNA PCR Primer, Index 14 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG	1516	0.25955659652132523	RNA PCR Primer, Index 16 (95% over 22bp)
TTCCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT	1010	0.1729235900306982	RNA PCR Primer, Index 14 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG	1009	0.1727523785554203	RNA PCR Primer, Index 14 (95% over 22bp)
TGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCTT	826	0.14142067857956112	RNA PCR Primer, Index 14 (96% over 25bp)
TGCTTTGGGATCGTTCTCTTAAAGTGCCAGTGTTTTAAATAGCGTTCTTGT	799	0.1367979687470573	No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	777	0.13303131629094309	No Hit
TGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG	762	0.1304631441617743	RNA PCR Primer, Index 14 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCC	749	0.12823739498316136	No Hit
TGTTTTGGGATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAA	704	0.120532878595655	No Hit
TTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG	685	0.11727986056537454	RNA PCR Primer, Index 14 (95% over 22bp)
TTTCCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC	648	0.11094503598009153	No Hit
CGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG	607	0.10392536549369685	RNA PCR Primer, Index 16 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC	598	0.10238446221619557	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8622209894996002	0.0
2	0.0	0.0	0.0	3.1139943123547913	0.0
3	0.0	0.0	0.0	4.286792918008537	0.0
4	0.0	0.0	0.0	5.534924572784567	0.0
5	0.0	0.0	0.0	7.638771180999635	0.0
6	0.0	0.0	0.0	8.942204142290432	0.0
7	0.0	0.0	0.0	10.09685433156472	0.0
8	0.0	0.0	0.0	12.017162238281859	0.0
9	0.0	0.0	0.0	12.872363557295063	0.0
10	0.0	0.0	0.0	14.529348215034764	0.0
11	0.0	0.0	0.0	17.535821720915024	0.0
12	0.0	0.0	0.0	20.062389461591273	0.0
13	0.0	0.0	0.0	20.869137933100827	0.0
14	0.0	0.0	0.0	21.163964093529405	0.0
15	0.0	0.0	0.0	21.581206458781693	0.0
16	0.0	0.0	0.0	22.67165234482676	0.0
17	0.0	0.0	0.0	24.26203573868335	0.0
18	0.0	0.0	0.0	26.173954283111872	0.0
19	0.0	0.0	0.0	27.238376024914693	0.0
20	0.0	0.0	0.0	28.33635521587199	0.0
21	0.0	0.0	0.0	29.803808770479034	0.0
22	0.0	0.0	0.0	31.190621720230176	0.0
23	0.0	0.0	0.0	32.55414990934352	0.0
24	3.4242295055583803E-4	0.0	0.0	33.506256923364035	0.0
25	3.4242295055583803E-4	0.0	0.0	34.348274958780834	0.0
26	3.4242295055583803E-4	0.0	0.0	35.11273419589675	0.0
27	3.4242295055583803E-4	0.0	0.0	35.846546578937904	0.0
28	3.4242295055583803E-4	0.0	0.0	36.64867234061496	0.0
29	6.848459011116761E-4	0.0	0.0	37.44822993016284	0.0
30	6.848459011116761E-4	0.0	0.0	38.381674893378054	0.0
31	6.848459011116761E-4	0.0	0.0	39.276083640229906	0.0
32	6.848459011116761E-4	0.0	0.0	40.046535278980535	0.0
33	6.848459011116761E-4	0.0	0.0	40.864583707858436	0.0
34	6.848459011116761E-4	0.0	0.0	41.59034915156153	0.0
35	6.848459011116761E-4	0.0	0.0	42.3892218952083	0.0
36	6.848459011116761E-4	0.0	0.0	43.0855389651636	0.0
37	6.848459011116761E-4	0.0	0.0	43.815755907223924	0.0
38	6.848459011116761E-4	0.0	0.0	44.48930185096726	0.0
39	6.848459011116761E-4	0.0	0.0	45.20291127992562	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCATA	30	2.1636606E-6	45.000004	19
CCGGGTT	30	2.1636606E-6	45.000004	5
GAGCTTA	30	2.1636606E-6	45.000004	9
TCGGGTA	60	0.0	45.000004	5
TACGCGC	30	2.1636606E-6	45.000004	34
GTAGATT	30	2.1636606E-6	45.000004	9
TGCGAAT	30	2.1636606E-6	45.000004	27
ACGGACG	30	2.1636606E-6	45.000004	11
GTCAAGG	30	2.1636606E-6	45.000004	26
ATGCGTC	30	2.1636606E-6	45.000004	18
ATGCGAA	30	2.1636606E-6	45.000004	26
AAGGACG	30	2.1636606E-6	45.000004	39
TAATGCG	30	2.1636606E-6	45.000004	24
CGGACGG	30	2.1636606E-6	45.000004	12
TATTTCG	20	7.0304336E-4	45.0	1
AGCGTGT	20	7.0304336E-4	45.0	16
GCCCATA	20	7.0304336E-4	45.0	23
GTCGCAA	20	7.0304336E-4	45.0	40
ACACGTA	35	1.2105556E-7	45.0	12
TCGCAAT	20	7.0304336E-4	45.0	41
>>END_MODULE