SRR2935282.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935282.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1212194
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGC	15908	1.3123311945117697	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCG	14951	1.2333834353247088	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTC	11319	0.933761427626271	No Hit
CCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	10268	0.8470591340989974	TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	7792	0.6428013997759435	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGCT	7379	0.6087309457067103	TruSeq Adapter, Index 21 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	6296	0.5193888107019173	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCGTGTTT	5119	0.4222921413569115	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTC	2825	0.2330485054372485	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCT	2807	0.23156359460614392	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTA	2620	0.21613702097189064	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCT	2499	0.20615512038502087	No Hit
TCCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG	2459	0.2028553185381218	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCG	2071	0.17084724062320059	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGC	1961	0.16177278554422805	No Hit
TGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGCTT	1700	0.1402415784932115	TruSeq Adapter, Index 14 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG	1653	0.13636431132310506	No Hit
CGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG	1553	0.12811480670585731	No Hit
GAATATGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTC	1356	0.11186328260987927	No Hit
TTCCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCT	1344	0.11087334205580955	No Hit
TGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG	1318	0.10872847085532514	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATCCG	45	3.8562575E-10	45.000004	1
CGGAATA	20	7.03344E-4	45.0	6
AACGTCA	20	7.03344E-4	45.0	23
CTGTCGT	20	7.03344E-4	45.0	22
TCGCAAA	20	7.03344E-4	45.0	14
CACGGAA	20	7.03344E-4	45.0	17
ACGTTCA	25	3.890932E-5	45.0	11
TTAGCCG	40	6.8157533E-9	45.0	1
GGCGTAT	25	3.890932E-5	45.0	8
CCGGACC	20	7.03344E-4	45.0	38
ATCACCG	20	7.03344E-4	45.0	17
ACGGGAT	185	0.0	45.0	5
ATATCGG	25	3.890932E-5	45.0	21
ACGATAG	20	7.03344E-4	45.0	23
CCGTACA	20	7.03344E-4	45.0	11
TCGGAAC	20	7.03344E-4	45.0	24
CGTAGTG	25	3.890932E-5	45.0	23
CGCTATA	160	0.0	45.0	21
GTCGTTT	20	7.03344E-4	45.0	9
AGTTCGG	20	7.03344E-4	45.0	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content