##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935282.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1212194
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.217880966247975	33.0	31.0	34.0	30.0	34.0
2	32.35475344705551	34.0	31.0	34.0	30.0	34.0
3	32.4308188293293	34.0	31.0	34.0	30.0	34.0
4	35.93698698393162	37.0	35.0	37.0	35.0	37.0
5	31.757424966630754	37.0	35.0	37.0	0.0	37.0
6	33.254724078819066	37.0	35.0	37.0	17.0	37.0
7	35.1376900067151	37.0	35.0	37.0	32.0	37.0
8	35.60179888697684	37.0	35.0	37.0	33.0	37.0
9	37.68564685190654	39.0	37.0	39.0	35.0	39.0
10	37.48474666596271	39.0	37.0	39.0	35.0	39.0
11	37.41128235249473	39.0	37.0	39.0	35.0	39.0
12	37.163994377137655	39.0	37.0	39.0	34.0	39.0
13	37.0219065595111	39.0	37.0	39.0	33.0	39.0
14	38.18474105629957	40.0	37.0	41.0	33.0	41.0
15	38.22191002430304	40.0	37.0	41.0	34.0	41.0
16	38.37098187253856	40.0	37.0	41.0	34.0	41.0
17	38.2999008409545	40.0	37.0	41.0	34.0	41.0
18	38.30427720315395	40.0	37.0	41.0	34.0	41.0
19	38.30829471190255	40.0	37.0	41.0	34.0	41.0
20	38.16587856399223	40.0	37.0	41.0	34.0	41.0
21	38.142458220383865	40.0	37.0	41.0	34.0	41.0
22	38.12407419934433	40.0	37.0	41.0	34.0	41.0
23	38.04278853054874	40.0	37.0	41.0	34.0	41.0
24	38.028007893126016	40.0	36.0	41.0	34.0	41.0
25	37.978187484841534	40.0	36.0	41.0	34.0	41.0
26	37.96579837880735	40.0	36.0	41.0	34.0	41.0
27	37.853382379388115	40.0	36.0	41.0	33.0	41.0
28	37.829142859971256	40.0	36.0	41.0	33.0	41.0
29	37.79642449970879	40.0	36.0	41.0	33.0	41.0
30	37.67976248026306	40.0	36.0	41.0	33.0	41.0
31	37.54384446713975	39.0	36.0	41.0	33.0	41.0
32	37.44252487638117	39.0	35.0	41.0	33.0	41.0
33	37.40259809898416	39.0	35.0	41.0	33.0	41.0
34	37.404533432767366	39.0	35.0	41.0	33.0	41.0
35	37.307111732940434	39.0	35.0	41.0	32.0	41.0
36	37.292929184602464	39.0	35.0	41.0	32.0	41.0
37	37.265732217780325	39.0	35.0	41.0	33.0	41.0
38	37.196099799207055	39.0	35.0	41.0	32.0	41.0
39	37.20666411481991	39.0	35.0	41.0	32.0	41.0
40	37.03623594903126	39.0	35.0	41.0	31.0	41.0
41	37.01071528154734	39.0	35.0	41.0	32.0	41.0
42	37.03790894856764	39.0	35.0	41.0	32.0	41.0
43	37.046978453943844	39.0	35.0	41.0	32.0	41.0
44	37.04233810759664	39.0	35.0	41.0	32.0	41.0
45	37.02130104587219	39.0	35.0	41.0	32.0	41.0
46	36.90546480183865	39.0	35.0	41.0	32.0	41.0
47	36.76891900141396	39.0	35.0	41.0	32.0	41.0
48	36.78474732592308	39.0	35.0	41.0	32.0	41.0
49	36.79716448027296	39.0	35.0	41.0	32.0	41.0
50	36.73581208948403	39.0	35.0	40.0	32.0	41.0
51	36.067404227376144	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	8.0
11	7.0
12	9.0
13	15.0
14	18.0
15	24.0
16	39.0
17	57.0
18	149.0
19	291.0
20	652.0
21	1161.0
22	2067.0
23	3026.0
24	3958.0
25	4933.0
26	6075.0
27	7293.0
28	9220.0
29	12048.0
30	16253.0
31	22431.0
32	30816.0
33	46198.0
34	75602.0
35	107679.0
36	104148.0
37	160915.0
38	256013.0
39	340745.0
40	342.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.725192502190247	4.459682196084125	50.44572073446989	15.369404567255737
2	18.228517877501456	11.475555892868632	49.08026273022305	21.215663499406862
3	19.796418725055563	11.38654373804853	47.7100200132982	21.107017523597708
4	18.128946356771277	4.048279400821981	53.56048619280413	24.26228804960262
5	20.461906262528935	16.28922433207886	40.537075748601296	22.71179365679091
6	19.72745286645537	11.994119753108826	49.28089068251452	18.99753669792129
7	75.68705999204748	2.457032455201065	16.05708327214951	5.798824280601949
8	75.1204840149349	8.284894992055728	9.453767301273558	7.140853691735812
9	70.38477339435767	5.0081917580849264	11.69392028008718	12.913114567470224
10	39.16501814066065	25.78935384930135	18.68092071071132	16.364707299326675
11	31.88227296950818	23.274987337010415	25.257095811396525	19.58564388208488
12	27.022984769764573	20.13308100848544	30.12809830769662	22.71583591405336
13	23.42479834085963	24.27862206874477	33.158388838750234	19.138190751645364
14	19.26251078622729	28.898509644495846	28.43661988097615	23.402359688300717
15	16.048338797255223	24.193404686048602	37.66014350838232	22.09811300831385
16	17.496869312997752	23.514470456049114	31.65565907767239	27.333001153280744
17	17.348955695210503	25.22484024834309	30.72008275903032	26.70612129741609
18	18.883116068880064	23.253456129959396	32.07819870416782	25.785229096992722
19	21.337261197465093	25.711643515806877	28.264700204752703	24.686395081975327
20	25.250496207702728	24.380585945813955	31.297960557468524	19.070957289014796
21	23.307325395110023	28.867079031904137	27.76304782897787	20.062547744007972
22	20.47411552936246	24.05176069177046	28.556980153341794	26.917143625525284
23	20.095710752569307	27.568442015056995	27.306272758320866	25.029574474052836
24	22.110652255332067	23.111564650542736	28.92895031653349	25.848832777591706
25	19.2498890441629	29.18823224665359	26.09046076783089	25.47141794135262
26	18.881548663002786	25.83307622377276	30.014337639024774	25.271037474199677
27	23.33982844330198	26.15356947815284	26.91458627909394	23.592015799451243
28	18.03226216265713	25.333981194429274	32.06598943733429	24.567767205579305
29	22.863502046702095	21.131766037449452	31.056662547414028	24.948069368434425
30	23.373321432048005	25.472077901722002	29.601614923023874	21.552985743206122
31	24.69348965594616	23.83768604695288	25.659259161487352	25.809565135613603
32	25.96234596112503	25.641770211698788	27.60284244931092	20.793041377865258
33	22.99054441780771	22.267722823244466	29.531741618915785	25.209991140032038
34	23.026347267846567	22.102980216038027	30.727837293370534	24.142835222744875
35	22.484272319447218	20.732737499113178	31.91419855237693	24.868791629062674
36	22.592505820025508	22.99978386297903	31.286163765865858	23.121546551129605
37	24.855839906813596	23.446494538002995	30.522754608585757	21.17491094659766
38	21.07368952494403	24.18004048856866	29.104747259927045	25.641522726560268
39	25.892390161970773	22.104547621915305	30.34266792279124	21.660394293322685
40	23.314997434404063	20.416039016857038	34.17415034227195	22.094813206466952
41	21.207249004697267	22.611397185599007	30.87558592106544	25.305767888638286
42	21.44136994573476	20.362994702168137	33.136445156468355	25.05919019562875
43	23.30460305858633	20.396405195867988	30.652436821168887	25.64655492437679
44	22.456471488887093	20.812262723623444	30.460883323956395	26.270382463533064
45	21.7433843097722	20.50232883515345	28.286643887034586	29.46764296803977
46	25.14308765758616	22.497224041696295	29.4160010691358	22.943687231581745
47	18.558085586960505	21.626076354114936	37.08193572975943	22.733902329165133
48	21.89220537306735	20.837176227567532	31.383507920349384	25.887110479015735
49	20.945162243007307	18.21465870974448	36.48698145676352	24.35319759048469
50	23.142170312672725	18.975098045362376	31.74706358883149	26.135668053133408
51	20.40052994817661	19.310687893192014	28.93513744499643	31.35364471363495
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	405.0
1	738.0
2	1071.0
3	1621.5
4	2172.0
5	1639.0
6	1106.0
7	1218.0
8	1330.0
9	1512.0
10	1694.0
11	2013.0
12	2332.0
13	2179.5
14	2027.0
15	2037.5
16	2048.0
17	2109.0
18	2170.0
19	2462.0
20	2754.0
21	2721.0
22	2688.0
23	3395.5
24	4103.0
25	5142.5
26	7382.5
27	8583.0
28	10787.0
29	12991.0
30	15393.5
31	17796.0
32	19988.0
33	22180.0
34	24157.0
35	26134.0
36	27457.5
37	28781.0
38	32819.0
39	36857.0
40	43118.0
41	49379.0
42	58819.5
43	68260.0
44	79777.0
45	91294.0
46	129533.5
47	167773.0
48	160953.5
49	154134.0
50	146078.0
51	138022.0
52	113427.5
53	88833.0
54	76643.5
55	64454.0
56	57471.5
57	50489.0
58	45720.0
59	40951.0
60	37512.0
61	34073.0
62	31100.0
63	28127.0
64	23176.0
65	18225.0
66	15148.5
67	12072.0
68	9935.0
69	7798.0
70	6368.0
71	4938.0
72	4091.5
73	3245.0
74	2851.5
75	1820.0
76	1182.0
77	881.0
78	580.0
79	432.5
80	285.0
81	240.0
82	195.0
83	102.0
84	9.0
85	11.0
86	13.0
87	6.5
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1212194.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.536051148403935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.15059211604606	16.230743116565122
2	12.63988016879862	6.20265492662679
3	5.902251908800473	4.344538641752795
4	3.287349931535858	3.226343442514639
5	2.1751357603677257	2.66846211355525
6	1.5137071361672116	2.2284237430021645
7	1.0502193317835262	1.8037764669178242
8	0.8266340288968973	1.622586785122039
9	0.6417258841256722	1.4170877204546006
>10	4.501385252674577	24.724262114963622
>50	1.1550491703855574	18.89171527245301
>100	0.13857878425046086	6.020540035810326
>500	0.009081619356140356	1.576377777185036
>1k	0.005718056631643928	2.5200917126916007
>5k	0.0013454250897985713	2.1941039518476186
>10k+	0.0013454250897985713	4.328292178537584
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGC	15908	1.3123311945117697	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCG	14951	1.2333834353247088	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTC	11319	0.933761427626271	No Hit
CCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	10268	0.8470591340989974	TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	7792	0.6428013997759435	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGCT	7379	0.6087309457067103	TruSeq Adapter, Index 21 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	6296	0.5193888107019173	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCGTGTTT	5119	0.4222921413569115	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTC	2825	0.2330485054372485	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCT	2807	0.23156359460614392	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTA	2620	0.21613702097189064	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCT	2499	0.20615512038502087	No Hit
TCCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG	2459	0.2028553185381218	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCG	2071	0.17084724062320059	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGC	1961	0.16177278554422805	No Hit
TGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGCTT	1700	0.1402415784932115	TruSeq Adapter, Index 14 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG	1653	0.13636431132310506	No Hit
CGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG	1553	0.12811480670585731	No Hit
GAATATGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTC	1356	0.11186328260987927	No Hit
TTCCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCT	1344	0.11087334205580955	No Hit
TGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG	1318	0.10872847085532514	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.249504617247734E-5	0.0	0.0	0.626137400449103	0.0
2	8.249504617247734E-5	0.0	0.0	2.750302344344222	0.0
3	8.249504617247734E-5	0.0	0.0	3.678784089015455	0.0
4	8.249504617247734E-5	0.0	0.0	4.791559766836001	0.0
5	8.249504617247734E-5	0.0	0.0	7.312278397682219	0.0
6	8.249504617247734E-5	0.0	0.0	8.34330148474584	0.0
7	8.249504617247734E-5	0.0	0.0	9.49377739866721	0.0
8	8.249504617247734E-5	0.0	0.0	11.056398563266276	0.0
9	8.249504617247734E-5	0.0	0.0	11.651352836262182	0.0
10	8.249504617247734E-5	0.0	0.0	14.31462290689444	0.0
11	8.249504617247734E-5	0.0	0.0	16.47508567110545	0.0
12	8.249504617247734E-5	0.0	0.0	19.89665020615512	0.0
13	8.249504617247734E-5	0.0	0.0	20.634320909029412	0.0
14	8.249504617247734E-5	0.0	0.0	20.941779946114234	0.0
15	8.249504617247734E-5	0.0	0.0	21.58466384093635	0.0
16	8.249504617247734E-5	0.0	0.0	22.524117426748525	0.0
17	8.249504617247734E-5	0.0	0.0	23.75774834721175	0.0
18	8.249504617247734E-5	0.0	0.0	25.067604690338346	0.0
19	1.6499009234495469E-4	0.0	0.0	26.468288079300837	0.0
20	1.6499009234495469E-4	0.0	0.0	27.377300993075366	0.0
21	1.6499009234495469E-4	0.0	0.0	28.560774925465726	0.0
22	1.6499009234495469E-4	0.0	0.0	29.72478002695938	0.0
23	1.6499009234495469E-4	0.0	0.0	30.894642276731282	0.0
24	1.6499009234495469E-4	0.0	0.0	31.745001212677177	0.0
25	1.6499009234495469E-4	0.0	0.0	32.57861365425006	0.0
26	1.6499009234495469E-4	0.0	0.0	33.254743052679686	0.0
27	3.2998018468990937E-4	0.0	0.0	33.95281613339119	0.0
28	3.2998018468990937E-4	0.0	0.0	34.64659947170173	0.0
29	4.124752308623867E-4	0.0	0.0	35.42782755895509	0.0
30	4.124752308623867E-4	0.0	0.0	36.28297120757898	0.0
31	4.124752308623867E-4	0.0	0.0	37.03417109802557	0.0
32	4.949702770348641E-4	0.0	0.0	37.758725088558435	0.0
33	4.949702770348641E-4	0.0	0.0	38.45547824853118	0.0
34	4.949702770348641E-4	0.0	0.0	39.13226760733018	0.0
35	4.949702770348641E-4	0.0	0.0	39.866143538080536	0.0
36	4.949702770348641E-4	0.0	0.0	40.581870558672954	0.0
37	4.949702770348641E-4	0.0	0.0	41.29050300529453	0.0
38	4.949702770348641E-4	0.0	0.0	41.94625612731956	0.0
39	4.949702770348641E-4	0.0	0.0	42.60984627873096	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATCCG	45	3.8562575E-10	45.000004	1
CGGAATA	20	7.03344E-4	45.0	6
AACGTCA	20	7.03344E-4	45.0	23
CTGTCGT	20	7.03344E-4	45.0	22
TCGCAAA	20	7.03344E-4	45.0	14
CACGGAA	20	7.03344E-4	45.0	17
ACGTTCA	25	3.890932E-5	45.0	11
TTAGCCG	40	6.8157533E-9	45.0	1
GGCGTAT	25	3.890932E-5	45.0	8
CCGGACC	20	7.03344E-4	45.0	38
ATCACCG	20	7.03344E-4	45.0	17
ACGGGAT	185	0.0	45.0	5
ATATCGG	25	3.890932E-5	45.0	21
ACGATAG	20	7.03344E-4	45.0	23
CCGTACA	20	7.03344E-4	45.0	11
TCGGAAC	20	7.03344E-4	45.0	24
CGTAGTG	25	3.890932E-5	45.0	23
CGCTATA	160	0.0	45.0	21
GTCGTTT	20	7.03344E-4	45.0	9
AGTTCGG	20	7.03344E-4	45.0	2
>>END_MODULE