SRR2935281.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935281.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	969944
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	7304	0.7530331648012669	TruSeq Adapter, Index 23 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGCT	5014	0.5169370602838926	Illumina PCR Primer Index 5 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	4404	0.45404683156965764	TruSeq Adapter, Index 23 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	3665	0.3778568659633958	TruSeq Adapter, Index 23 (95% over 24bp)
TCCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTG	1900	0.1958875976345026	Illumina PCR Primer Index 5 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	1215	0.12526496375048457	No Hit
CGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTG	1188	0.12248129788936268	Illumina PCR Primer Index 5 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTG	1089	0.11227452306524913	TruSeq Adapter, Index 23 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGA	70	0.0	45.000004	24
TAGCGAT	25	3.8903527E-5	45.000004	15
TCGATAC	30	2.1651504E-6	45.000004	25
CCGTACA	30	2.1651504E-6	45.000004	21
TGCGATG	25	3.8903527E-5	45.000004	1
AGGTTCG	25	3.8903527E-5	45.000004	33
TACGAGT	25	3.8903527E-5	45.000004	18
GTCGTAA	25	3.8903527E-5	45.000004	29
CGTACAG	30	2.1651504E-6	45.000004	22
TGCTCGA	25	3.8903527E-5	45.000004	16
CTCGATA	30	2.1651504E-6	45.000004	24
CGTCGCA	25	3.8903527E-5	45.000004	26
CCGAACG	25	3.8903527E-5	45.000004	37
CGCACGA	25	3.8903527E-5	45.000004	39
TCGAGAC	35	1.2116652E-7	45.000004	34
CGACTCG	25	3.8903527E-5	45.000004	21
TGTACGA	30	2.1651504E-6	45.000004	21
ATCGATC	25	3.8903527E-5	45.000004	14
AGTCGTA	50	2.1827873E-11	45.000004	28
TACGTAA	30	2.1651504E-6	45.000004	18