##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935281.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	969944
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.342067170888214	33.0	31.0	34.0	30.0	34.0
2	32.4838990704618	34.0	31.0	34.0	31.0	34.0
3	32.54643979446236	34.0	31.0	34.0	31.0	34.0
4	35.98103395659955	37.0	35.0	37.0	35.0	37.0
5	31.946805176381318	37.0	35.0	37.0	0.0	37.0
6	33.583584206923284	37.0	35.0	37.0	17.0	37.0
7	35.30493925422498	37.0	35.0	37.0	32.0	37.0
8	35.82158042113772	37.0	35.0	37.0	35.0	37.0
9	37.852904909974185	39.0	38.0	39.0	35.0	39.0
10	37.55924465742352	39.0	37.0	39.0	35.0	39.0
11	37.453433394092855	39.0	37.0	39.0	35.0	39.0
12	37.14914572387684	39.0	37.0	39.0	34.0	39.0
13	37.019062956211904	39.0	37.0	39.0	33.0	39.0
14	38.135445963890696	40.0	37.0	41.0	33.0	41.0
15	38.25495080128337	40.0	37.0	41.0	34.0	41.0
16	38.33392133978869	40.0	37.0	41.0	34.0	41.0
17	38.338763887399686	40.0	37.0	41.0	34.0	41.0
18	38.32706836683355	40.0	37.0	41.0	34.0	41.0
19	38.35062127298071	40.0	37.0	41.0	34.0	41.0
20	38.28801456578937	40.0	37.0	41.0	34.0	41.0
21	38.224792359146505	40.0	37.0	41.0	34.0	41.0
22	38.19308331202626	40.0	37.0	41.0	34.0	41.0
23	38.16389812195343	40.0	36.0	41.0	34.0	41.0
24	38.13143851603804	40.0	36.0	41.0	34.0	41.0
25	38.05229477165692	40.0	36.0	41.0	34.0	41.0
26	37.99724520178484	40.0	36.0	41.0	34.0	41.0
27	37.967089852610044	40.0	36.0	41.0	34.0	41.0
28	37.95118687264419	40.0	36.0	41.0	34.0	41.0
29	37.90258303572165	40.0	36.0	41.0	34.0	41.0
30	37.84488589031944	40.0	36.0	41.0	34.0	41.0
31	37.642676278218126	40.0	36.0	41.0	33.0	41.0
32	37.69651237597222	40.0	35.0	41.0	33.0	41.0
33	37.714859826959085	40.0	35.0	41.0	33.0	41.0
34	37.667347805646514	40.0	35.0	41.0	33.0	41.0
35	37.580897453873625	40.0	35.0	41.0	33.0	41.0
36	37.589347426243165	40.0	35.0	41.0	33.0	41.0
37	37.555206279950184	40.0	35.0	41.0	33.0	41.0
38	37.55747754509539	40.0	35.0	41.0	33.0	41.0
39	37.54611297147052	39.0	35.0	41.0	33.0	41.0
40	37.43113107560849	39.0	35.0	41.0	33.0	41.0
41	37.39088132923138	39.0	35.0	41.0	33.0	41.0
42	37.398291035358746	39.0	35.0	41.0	33.0	41.0
43	37.37952912745478	39.0	35.0	41.0	33.0	41.0
44	37.30893020627995	39.0	35.0	41.0	33.0	41.0
45	37.2920178896926	39.0	35.0	41.0	33.0	41.0
46	37.197235098108756	39.0	35.0	41.0	33.0	41.0
47	37.15696163902246	39.0	35.0	41.0	33.0	41.0
48	37.14429905231642	39.0	35.0	41.0	33.0	41.0
49	37.149973606723684	39.0	35.0	41.0	33.0	41.0
50	37.06163036216524	39.0	35.0	41.0	33.0	41.0
51	36.423007926230795	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	10.0
10	12.0
11	9.0
12	11.0
13	13.0
14	20.0
15	19.0
16	57.0
17	62.0
18	126.0
19	196.0
20	335.0
21	498.0
22	834.0
23	1272.0
24	1923.0
25	2764.0
26	3871.0
27	5117.0
28	6587.0
29	8491.0
30	11460.0
31	15696.0
32	22299.0
33	35330.0
34	63520.0
35	94301.0
36	77110.0
37	118142.0
38	196586.0
39	302944.0
40	326.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.474802669020065	4.100133615961334	55.89178344316785	17.533280271850746
2	19.136568709121352	4.163230042146763	54.39448050609107	22.305720742640812
3	20.82419191211039	4.082606830909826	52.596747853484324	22.496453403495458
4	19.039346601453282	5.01781546151118	50.6061174665754	25.33672047046015
5	16.322798017205116	16.082371765792665	44.55133492242851	23.043495294573706
6	21.29463144263999	6.409339095865328	53.42772366239701	18.86830579909768
7	82.82622501917636	2.141566935823099	9.664166178665985	5.3680418663345515
8	84.00206609866136	2.6022120864709715	7.373621569905067	6.022100244962596
9	78.95301172026427	4.998535997954521	10.075942528640828	5.972509753140388
10	39.48279488300355	27.48385473800549	17.548229588512328	15.485120790478627
11	30.681668220020953	25.655914155868793	23.715389754460052	19.947027869650206
12	28.574020768209294	20.20951725048044	30.865287068119397	20.351174913190864
13	24.562242768654684	25.34352498700956	30.36340242323268	19.730829821103075
14	18.518285591745503	28.498037000074234	31.172108905256383	21.81156850292388
15	17.463482427851503	24.412955799510076	36.57159588594806	21.551965886690365
16	19.13945547371807	23.35691545078891	33.99103453395247	23.512594541540544
17	19.33616786123735	22.679247461709128	29.020850688287158	28.96373398876636
18	20.18528904761512	23.14411966051648	32.20515823593939	24.465433055929005
19	22.02745725526422	24.89061224153147	30.53413392938149	22.54779657382282
20	24.973091230009157	24.698745494585253	29.05961581287167	21.268547462533917
21	23.865295316018244	26.69401532459606	28.533915360062025	20.906773999323672
22	21.7218726029544	24.916902419108734	27.47158598846944	25.889638989467436
23	20.76790000247437	25.563022195095797	27.316628588866987	26.352449213562846
24	21.99044480918486	23.634766543223115	30.137203797332628	24.237584850259395
25	20.413858944433905	24.63977301782371	28.69464628885791	26.25172174888447
26	18.79953894245441	27.119606905140913	29.4148940557393	24.665960096665373
27	21.35401631434392	25.53415454912861	29.513765743176922	23.598063393350543
28	18.932227015167886	24.340889783327697	32.01370388393557	24.713179317568848
29	19.69608554720685	22.377889857558785	33.455127306318715	24.47089728891565
30	23.157316298672914	23.981899985978572	30.342782676113263	22.518001039235255
31	22.929983586681292	25.2493958414094	28.010483079435517	23.810137492473793
32	22.558725039796112	24.789987875588693	29.989257111750785	22.66202997286441
33	24.05716206296446	23.991281970917907	27.21497323556824	24.73658273054939
34	20.476233679470155	22.5983149542654	30.81260361422927	26.11284775203517
35	19.262142969078628	22.32541260113986	32.675391568997796	25.737052860783717
36	24.230264840032	22.74203459168777	29.014252369208943	24.013448199071284
37	21.414638370875018	25.12516186501489	30.556403256270464	22.903796507839626
38	19.977441996651354	25.05660120584281	29.385717113565317	25.580239683940516
39	22.752138267776285	23.29423141954587	30.543515914320828	23.410114398357017
40	23.427228788466138	21.602587365868544	32.11092599160364	22.859257854061678
41	19.22286235081613	22.163135191310012	30.83734731077258	27.776655147101277
42	20.787694959709015	21.83734318682316	29.671506808640498	27.70345504482733
43	22.26633702564272	21.71754245605932	28.306995043012794	27.709125475285173
44	20.747692650297335	21.977145072292835	29.87038426960732	27.40477800780251
45	21.426494725468686	22.24097473668583	28.729493661489734	27.603036876355745
46	22.851422350156298	22.25870771920853	31.530067715249537	23.35980221538563
47	19.411429938223236	22.21963329841723	33.346873633941755	25.02206312941778
48	19.53638560576693	20.755631252938315	33.06087774139538	26.647105399899374
49	21.00234652722219	19.374417492143873	33.73112262151217	25.892113359121765
50	21.451135323276397	19.770419735572364	31.919471639599813	26.85897330155143
51	19.178426795773778	20.382516928812386	29.737696196893843	30.701360078519997
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	326.0
1	545.5
2	765.0
3	1203.5
4	1642.0
5	1239.0
6	836.0
7	909.5
8	983.0
9	1218.5
10	1454.0
11	1538.0
12	1622.0
13	1637.5
14	1653.0
15	1745.5
16	1838.0
17	1898.0
18	1958.0
19	2329.0
20	2700.0
21	2822.5
22	2945.0
23	2936.0
24	2927.0
25	3903.0
26	5967.5
27	7056.0
28	8724.5
29	10393.0
30	11351.5
31	12310.0
32	14999.5
33	17689.0
34	20208.0
35	22727.0
36	25409.0
37	28091.0
38	30832.5
39	33574.0
40	37963.0
41	42352.0
42	52057.5
43	61763.0
44	66447.0
45	71131.0
46	83966.5
47	96802.0
48	108510.0
49	120218.0
50	116364.5
51	112511.0
52	92636.0
53	72761.0
54	63769.5
55	54778.0
56	50000.5
57	45223.0
58	40605.5
59	35988.0
60	33782.5
61	31577.0
62	26945.5
63	22314.0
64	19682.5
65	17051.0
66	13331.5
67	9612.0
68	7921.0
69	6230.0
70	5396.5
71	4563.0
72	3771.5
73	2980.0
74	2579.5
75	1440.5
76	702.0
77	594.5
78	487.0
79	363.5
80	240.0
81	163.5
82	87.0
83	54.5
84	22.0
85	13.0
86	4.0
87	2.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	969944.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.489280741869994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.75519942356398	15.858082581794363
2	13.130909110448233	6.431330392034903
3	6.121784048417219	4.497542646083722
4	3.456791647726651	3.3861736450931725
5	2.2175339644867753	2.7152905905474305
6	1.5230134941871691	2.237850301968358
7	1.161548842888317	1.9911846996220364
8	0.8269372815798015	1.6200879395621246
9	0.6511068466369528	1.4350624524221427
>10	4.53985977549047	24.062372321212997
>50	1.3971331316054303	23.89014845466308
>100	0.20754976079699264	7.988553476898871
>500	0.007230217076944413	1.2013191280531894
>1k	0.0025518413212744988	1.4020250375172518
>5k	8.506137737581663E-4	1.282976332526373
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	7304	0.7530331648012669	TruSeq Adapter, Index 23 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGCT	5014	0.5169370602838926	Illumina PCR Primer Index 5 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	4404	0.45404683156965764	TruSeq Adapter, Index 23 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	3665	0.3778568659633958	TruSeq Adapter, Index 23 (95% over 24bp)
TCCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTG	1900	0.1958875976345026	Illumina PCR Primer Index 5 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	1215	0.12526496375048457	No Hit
CGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTG	1188	0.12248129788936268	Illumina PCR Primer Index 5 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTG	1089	0.11227452306524913	TruSeq Adapter, Index 23 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5318863769454731	0.0
2	0.0	0.0	0.0	2.2091997063748012	0.0
3	0.0	0.0	0.0	3.081105713319532	0.0
4	0.0	0.0	0.0	3.8889874054584594	0.0
5	0.0	0.0	0.0	5.348556205306698	0.0
6	0.0	0.0	0.0	6.2788160965993915	0.0
7	0.0	0.0	0.0	7.096698366091238	0.0
8	0.0	0.0	0.0	8.429868116097424	0.0
9	0.0	0.0	0.0	9.014644144404214	0.0
10	0.0	0.0	0.0	10.246467837318443	0.0
11	0.0	0.0	0.0	12.492679989772606	0.0
12	0.0	0.0	0.0	14.540220878731143	0.0
13	0.0	0.0	0.0	15.156545120130646	0.0
14	0.0	0.0	0.0	15.381094166261144	0.0
15	0.0	0.0	0.0	15.730186484992949	0.0
16	0.0	0.0	0.0	16.590751631022	0.0
17	0.0	0.0	0.0	17.840823800136913	0.0
18	0.0	0.0	0.0	19.294206675849328	0.0
19	0.0	0.0	0.0	20.181886789340414	0.0
20	1.0309873559710663E-4	0.0	0.0	21.091320736042494	0.0
21	1.0309873559710663E-4	0.0	0.0	22.3809828196267	0.0
22	1.0309873559710663E-4	0.0	0.0	23.590949580594344	0.0
23	1.0309873559710663E-4	0.0	0.0	24.7719455968592	0.0
24	2.0619747119421326E-4	0.0	0.0	25.709319300908092	0.0
25	2.0619747119421326E-4	0.0	0.0	26.47946685581848	0.0
26	2.0619747119421326E-4	0.0	0.0	27.243737782799833	0.0
27	2.0619747119421326E-4	0.0	0.0	27.95295398497233	0.0
28	2.0619747119421326E-4	0.0	0.0	28.707018137129566	0.0
29	2.0619747119421326E-4	0.0	0.0	29.488403454220038	0.0
30	2.0619747119421326E-4	0.0	0.0	30.343607465998037	0.0
31	2.0619747119421326E-4	0.0	0.0	31.16880974571728	0.0
32	2.0619747119421326E-4	0.0	0.0	31.88895441386307	0.0
33	2.0619747119421326E-4	0.0	0.0	32.60610921867654	0.0
34	2.0619747119421326E-4	0.0	0.0	33.285942281203866	0.0
35	3.0929620679131994E-4	0.0	0.0	34.09454566449197	0.0
36	3.0929620679131994E-4	0.0	0.0	34.804277360342454	0.0
37	3.0929620679131994E-4	0.0	0.0	35.52153526389152	0.0
38	3.0929620679131994E-4	0.0	0.0	36.26642362858062	0.0
39	3.0929620679131994E-4	0.0	0.0	37.00430127924911	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGA	70	0.0	45.000004	24
TAGCGAT	25	3.8903527E-5	45.000004	15
TCGATAC	30	2.1651504E-6	45.000004	25
CCGTACA	30	2.1651504E-6	45.000004	21
TGCGATG	25	3.8903527E-5	45.000004	1
AGGTTCG	25	3.8903527E-5	45.000004	33
TACGAGT	25	3.8903527E-5	45.000004	18
GTCGTAA	25	3.8903527E-5	45.000004	29
CGTACAG	30	2.1651504E-6	45.000004	22
TGCTCGA	25	3.8903527E-5	45.000004	16
CTCGATA	30	2.1651504E-6	45.000004	24
CGTCGCA	25	3.8903527E-5	45.000004	26
CCGAACG	25	3.8903527E-5	45.000004	37
CGCACGA	25	3.8903527E-5	45.000004	39
TCGAGAC	35	1.2116652E-7	45.000004	34
CGACTCG	25	3.8903527E-5	45.000004	21
TGTACGA	30	2.1651504E-6	45.000004	21
ATCGATC	25	3.8903527E-5	45.000004	14
AGTCGTA	50	2.1827873E-11	45.000004	28
TACGTAA	30	2.1651504E-6	45.000004	18
>>END_MODULE