##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935280.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1259475
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2423128684571	33.0	31.0	34.0	30.0	34.0
2	32.388130768772704	34.0	31.0	34.0	30.0	34.0
3	32.446154151531395	34.0	31.0	34.0	30.0	34.0
4	35.916431052621135	37.0	35.0	37.0	35.0	37.0
5	31.793473471089143	37.0	35.0	37.0	0.0	37.0
6	33.48817086484448	37.0	35.0	37.0	17.0	37.0
7	35.20394211873995	37.0	35.0	37.0	32.0	37.0
8	35.74104210087536	37.0	35.0	37.0	35.0	37.0
9	37.762030211000614	39.0	38.0	39.0	35.0	39.0
10	37.49220826137875	39.0	37.0	39.0	35.0	39.0
11	37.370142321205265	39.0	37.0	39.0	35.0	39.0
12	37.10260306873896	39.0	37.0	39.0	34.0	39.0
13	36.98111355922111	39.0	37.0	39.0	33.0	39.0
14	38.105782171142735	40.0	37.0	41.0	33.0	41.0
15	38.22575120585959	40.0	37.0	41.0	34.0	41.0
16	38.30574326604339	40.0	37.0	41.0	34.0	41.0
17	38.32536096389369	40.0	37.0	41.0	34.0	41.0
18	38.32016038428711	40.0	37.0	41.0	34.0	41.0
19	38.321054407590466	40.0	37.0	41.0	34.0	41.0
20	38.24513467913218	40.0	37.0	41.0	34.0	41.0
21	38.18247523769825	40.0	37.0	41.0	34.0	41.0
22	38.14012425812342	40.0	37.0	41.0	34.0	41.0
23	38.11047142658647	40.0	37.0	41.0	34.0	41.0
24	38.10328192302348	40.0	36.0	41.0	34.0	41.0
25	38.00193255126144	40.0	36.0	41.0	34.0	41.0
26	37.940229063697174	40.0	36.0	41.0	34.0	41.0
27	37.83799202048473	40.0	36.0	41.0	33.0	41.0
28	37.776724428829475	40.0	36.0	41.0	33.0	41.0
29	37.731074455626356	40.0	36.0	41.0	33.0	41.0
30	37.69522856745866	40.0	36.0	41.0	33.0	41.0
31	37.45860537128566	39.0	35.0	41.0	33.0	41.0
32	37.50204886956867	39.0	35.0	41.0	33.0	41.0
33	37.48424303777367	39.0	35.0	41.0	33.0	41.0
34	37.47242144544354	39.0	35.0	41.0	33.0	41.0
35	37.407681772166974	39.0	35.0	41.0	33.0	41.0
36	37.35046745667838	39.0	35.0	41.0	33.0	41.0
37	37.291036344508626	39.0	35.0	41.0	33.0	41.0
38	37.292317036860595	39.0	35.0	41.0	33.0	41.0
39	37.309656801445044	39.0	35.0	41.0	33.0	41.0
40	37.23977530320173	39.0	35.0	41.0	33.0	41.0
41	37.19583239048016	39.0	35.0	41.0	33.0	41.0
42	37.152508783421666	39.0	35.0	41.0	33.0	41.0
43	37.079179023005615	39.0	35.0	41.0	32.0	41.0
44	37.011454772822006	39.0	35.0	41.0	32.0	41.0
45	37.00436769288791	39.0	35.0	41.0	32.0	41.0
46	36.885172790249904	39.0	35.0	41.0	32.0	41.0
47	36.7971567518212	39.0	35.0	40.0	32.0	41.0
48	36.770963298199646	39.0	35.0	40.0	32.0	41.0
49	36.750258639512495	39.0	35.0	40.0	32.0	41.0
50	36.661327140276704	38.0	35.0	40.0	32.0	41.0
51	36.01390182417277	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	11.0
11	10.0
12	13.0
13	15.0
14	25.0
15	37.0
16	52.0
17	80.0
18	155.0
19	254.0
20	487.0
21	860.0
22	1300.0
23	1846.0
24	2912.0
25	4294.0
26	5758.0
27	7358.0
28	9700.0
29	12395.0
30	16892.0
31	22680.0
32	31606.0
33	48748.0
34	82246.0
35	118192.0
36	108446.0
37	169446.0
38	268479.0
39	344857.0
40	314.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.40086147005697	4.595327418170269	54.842295400861474	17.161515710911292
2	19.57053534210683	4.977867762361302	52.506242680481954	22.94535421504992
3	21.1576252009766	4.660513309116894	50.373608050973615	23.808253438932887
4	19.771095099148457	4.900295758153199	49.8618074991564	25.466801643541952
5	16.048432878778858	16.930784652335298	43.07556720061931	23.94521526826654
6	21.48339585938586	6.087457075368706	52.51553226542806	19.913614799817385
7	81.27854860159988	2.7783798805057662	10.237281406935429	5.705790110958931
8	82.01401377558109	3.0334861747950534	8.365628535699399	6.586871513924453
9	77.19065483634054	5.352309494035213	11.141547073185256	6.315488596438993
10	40.014926854443324	26.289525397487047	17.307052541733658	16.388495206335975
11	29.412175708132356	25.221699517656166	24.82383532821215	20.542289445999327
12	26.80243752357133	20.696639472796203	31.548224458603784	20.952698545028685
13	22.845630123662637	24.886162885329206	32.457254014569564	19.810952976438596
14	18.492070108576986	26.79068659560531	32.29615514400842	22.421088151809286
15	16.294487782607835	24.36793108239544	37.33515949105779	22.002421643938945
16	18.316758967029916	24.411282478810612	33.64536810972826	23.626590444431212
17	18.62561781694754	22.945195418726055	29.81591536155938	28.613271402767026
18	20.209055360368406	22.621171519879315	32.673296413188034	24.496476706564245
19	22.8835824450664	23.929891423013558	29.721510947021578	23.46501518489847
20	24.655273030429345	25.447984279163936	28.989380495841523	20.907362194565195
21	23.546160106393536	26.213779551003398	29.1846999741956	21.05536036840747
22	21.722701919450564	24.490124853609636	28.1963516544592	25.5908215724806
23	21.169614323428412	24.886242283491136	27.84811131622303	26.096032076857423
24	21.951051033168582	23.55719645090216	29.255761329125228	25.235991186804025
25	20.285912781119116	24.9878719307648	27.799519641120305	26.926695646995775
26	20.547609122848804	27.52170547251831	27.26644038190516	24.664245022727723
27	20.67472558010282	28.878620059945614	27.38656980090911	23.06008455904246
28	18.140971436511244	27.536711725123563	30.664443518132558	23.657873320232635
29	19.207447547589275	26.54510808074793	28.549117687925524	25.69832668373727
30	21.257111097878084	27.35306377657357	26.871593322614583	24.518231802933762
31	21.580658607753232	29.0151848984696	23.657793922070706	25.746362571706467
32	22.050775124555866	29.498719704638837	23.011731078425534	25.438774092379763
33	20.99184183886143	28.288294725977092	23.81484348637329	26.905019948788183
34	19.544095754183292	27.094463963159253	25.588360229460687	27.773080053196768
35	20.599456122590762	26.02870243553862	24.900772147124794	28.471069294745828
36	22.332003414120962	28.85019551797376	24.899581174695808	23.91821989320947
37	18.919629210583775	31.77871732269398	25.6583894082852	23.643264058437047
38	18.760594692232875	31.43103277159134	24.1526826653963	25.65568987077949
39	20.37674427836996	26.899065086643244	25.802020683221183	26.922169951765618
40	21.76978502947657	23.129002163599914	26.065622580837257	29.035590226086267
41	18.53010182814268	25.050278886043785	24.631135989201848	31.788483296611687
42	20.57849500784057	26.581393040751106	24.958018221878163	27.882093729530162
43	21.173822426010837	27.57863395462395	25.942317235356	25.30522638400921
44	20.409773913733897	26.029655213481806	26.4160066694456	27.144564203338696
45	21.07306615851843	25.278866194247602	24.376664880208025	29.271402767025943
46	21.280454157486254	25.28585323249767	26.930824351416266	26.502868258599815
47	18.15073741042895	26.340022628476152	28.88949760813037	26.619742352964533
48	18.10413068937454	25.899442227912424	28.564520931340436	27.431906151372598
49	19.78967426904067	23.813334921296573	29.787570217749458	26.609420591913295
50	20.27948153000258	23.272554040373965	27.200539907501142	29.247424522122312
51	17.96462811885905	23.55934020127434	25.45425673395661	33.02177494591
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	445.0
1	723.0
2	1001.0
3	1653.0
4	2305.0
5	1758.0
6	1211.0
7	1319.5
8	1428.0
9	1670.5
10	1913.0
11	2149.0
12	2385.0
13	2294.5
14	2204.0
15	2188.0
16	2172.0
17	2397.0
18	2622.0
19	2446.5
20	2271.0
21	2692.0
22	3113.0
23	4486.5
24	5860.0
25	5701.0
26	7029.5
27	8517.0
28	9573.5
29	10630.0
30	12547.5
31	14465.0
32	18525.5
33	22586.0
34	24046.5
35	25507.0
36	28807.5
37	32108.0
38	35777.5
39	39447.0
40	47239.5
41	55032.0
42	66511.5
43	77991.0
44	86677.0
45	95363.0
46	111973.5
47	128584.0
48	146972.0
49	165360.0
50	155915.5
51	146471.0
52	123914.5
53	101358.0
54	86712.0
55	72066.0
56	64012.0
57	55958.0
58	50510.0
59	45062.0
60	41826.5
61	38591.0
62	33744.0
63	28897.0
64	26226.0
65	23555.0
66	18942.0
67	14329.0
68	11041.0
69	7753.0
70	7025.0
71	6297.0
72	5385.0
73	4473.0
74	3199.0
75	1532.0
76	1139.0
77	1052.0
78	965.0
79	570.0
80	175.0
81	256.5
82	338.0
83	197.5
84	57.0
85	29.0
86	1.0
87	1.0
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1259475.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.272521736078858
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.95694031454062	16.416256860084047
2	12.92688873588049	6.533901531148254
3	6.022299089039531	4.565960538867583
4	3.4621181222957347	3.4998582199436594
5	2.255973158208784	2.850706533842098
6	1.6145095580350943	2.4481636739129393
7	1.1944059708204784	2.112995560148404
8	0.9227506967871439	1.8656189633228004
9	0.703105346526838	1.5992320637567492
>10	4.495718667869192	23.518689807324613
>50	1.2249402591368441	21.647844768235124
>100	0.20749893816625098	8.260363536465665
>500	0.008776390131820128	1.4854364008828462
>1k	0.002820982542370755	1.0431008173435004
>5k	0.0012537700188314468	2.151870724721764
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC	7806	0.6197820520454952	No Hit
CTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGCT	7531	0.5979475575140436	No Hit
TCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC	6461	0.5129915242462136	No Hit
GCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC	5367	0.42612993509200264	No Hit
TCCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTG	2513	0.19952758093650133	No Hit
TTCCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCT	1545	0.1226701601857917	No Hit
GCCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTG	1488	0.11814446495563627	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	1398	0.11099863038170667	No Hit
CGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTG	1389	0.1102840469243137	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCC	1318	0.10464677742710257	No Hit
TGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGCTT	1272	0.1009944619782052	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.381944857976538E-4	0.0	0.0	0.6207348299886858	0.0
2	2.381944857976538E-4	0.0	0.0	2.2970682228706405	0.0
3	2.381944857976538E-4	0.0	0.0	3.1702892078048395	0.0
4	2.381944857976538E-4	0.0	0.0	4.156493777169058	0.0
5	2.381944857976538E-4	0.0	0.0	5.763671371007761	0.0
6	2.381944857976538E-4	0.0	0.0	6.860318783620159	0.0
7	2.381944857976538E-4	0.0	0.0	7.833740248913237	0.0
8	2.381944857976538E-4	0.0	0.0	9.33944699180214	0.0
9	2.381944857976538E-4	0.0	0.0	10.024494332956191	0.0
10	2.381944857976538E-4	0.0	0.0	11.354334147164494	0.0
11	2.381944857976538E-4	0.0	0.0	13.985033446475715	0.0
12	2.381944857976538E-4	0.0	0.0	16.350622283094147	0.0
13	2.381944857976538E-4	0.0	0.0	17.09609162547887	0.0
14	2.381944857976538E-4	0.0	0.0	17.363266440381906	0.0
15	2.381944857976538E-4	0.0	0.0	17.74183687647631	0.0
16	3.1759264773020503E-4	0.0	0.0	18.726691677087675	0.0
17	3.1759264773020503E-4	0.0	0.0	20.12290835467159	0.0
18	3.1759264773020503E-4	0.0	0.0	21.772484566982275	0.0
19	3.1759264773020503E-4	0.0	0.0	22.78878103971893	0.0
20	3.1759264773020503E-4	0.0	0.0	23.860973818456102	0.0
21	3.1759264773020503E-4	0.0	0.0	25.31014907004903	0.0
22	3.9699080966275633E-4	0.0	0.0	26.690486115246433	0.0
23	3.9699080966275633E-4	0.0	0.0	28.012941900395006	0.0
24	3.9699080966275633E-4	0.0	0.0	29.00430735028484	0.0
25	3.9699080966275633E-4	0.0	0.0	29.86720657416781	0.0
26	3.9699080966275633E-4	0.0	0.0	30.676512038746303	0.0
27	3.9699080966275633E-4	0.0	0.0	31.42611008555152	0.0
28	4.763889715953076E-4	0.0	0.0	32.2063558228627	0.0
29	4.763889715953076E-4	0.0	0.0	33.02796800254074	0.0
30	4.763889715953076E-4	0.0	0.0	33.98074594573136	0.0
31	4.763889715953076E-4	0.0	0.0	34.83173544532444	0.0
32	4.763889715953076E-4	0.0	0.0	35.638500168721094	0.0
33	4.763889715953076E-4	0.0	0.0	36.39063895670815	0.0
34	4.763889715953076E-4	0.0	0.0	37.17969788999385	0.0
35	4.763889715953076E-4	0.0	0.0	38.033942714226164	0.0
36	4.763889715953076E-4	0.0	0.0	38.86611484944124	0.0
37	4.763889715953076E-4	0.0	0.0	39.65644415331785	0.0
38	4.763889715953076E-4	0.0	0.0	40.44439151233649	0.0
39	5.557871335278589E-4	0.0	0.0	41.23059211179261	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTACG	20	7.0335437E-4	45.0	1
AAATCGG	20	7.0335437E-4	45.0	10
CGAAAAT	25	3.89102E-5	45.0	22
ACGTTGA	20	7.0335437E-4	45.0	28
GCGGTAT	25	3.89102E-5	45.0	30
GCACGAC	95	0.0	45.0	16
TCGATTA	20	7.0335437E-4	45.0	45
TACGGCA	25	3.89102E-5	45.0	42
CGTTAGC	20	7.0335437E-4	45.0	25
CGTTAAC	20	7.0335437E-4	45.0	28
TATTCGA	20	7.0335437E-4	45.0	17
AACGCAC	20	7.0335437E-4	45.0	42
TCCGTTA	20	7.0335437E-4	45.0	23
CGCGTTG	25	3.89102E-5	45.0	14
TGTACGG	95	0.0	45.0	2
CCGTTAG	20	7.0335437E-4	45.0	24
AATCGAA	20	7.0335437E-4	45.0	34
GACTACG	20	7.0335437E-4	45.0	42
CCCGATA	20	7.0335437E-4	45.0	23
TAATCGG	20	7.0335437E-4	45.0	44
>>END_MODULE