##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935279.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2121121
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.339722722088936	33.0	31.0	34.0	30.0	34.0
2	32.48432173364933	34.0	31.0	34.0	31.0	34.0
3	32.53776092924449	34.0	31.0	34.0	31.0	34.0
4	35.992743459708336	37.0	35.0	37.0	35.0	37.0
5	31.915056236772912	37.0	35.0	37.0	0.0	37.0
6	33.555046128910135	37.0	35.0	37.0	17.0	37.0
7	35.30561151391175	37.0	35.0	37.0	32.0	37.0
8	35.8366255390428	37.0	35.0	37.0	35.0	37.0
9	37.884880211925676	39.0	38.0	39.0	35.0	39.0
10	37.54817381940964	39.0	37.0	39.0	35.0	39.0
11	37.413446474764996	39.0	37.0	39.0	35.0	39.0
12	37.232695824519205	39.0	37.0	39.0	34.0	39.0
13	37.19512323907971	39.0	37.0	39.0	34.0	39.0
14	38.37523271892551	40.0	38.0	41.0	34.0	41.0
15	38.45945657979908	40.0	38.0	41.0	34.0	41.0
16	38.514882460736565	40.0	38.0	41.0	34.0	41.0
17	38.515208231873615	40.0	38.0	41.0	34.0	41.0
18	38.485648862087544	40.0	38.0	41.0	34.0	41.0
19	38.52368063868115	40.0	38.0	41.0	34.0	41.0
20	38.46641516443428	40.0	38.0	41.0	34.0	41.0
21	38.40664158244626	40.0	37.0	41.0	34.0	41.0
22	38.38920787640121	40.0	37.0	41.0	34.0	41.0
23	38.332533127530205	40.0	37.0	41.0	34.0	41.0
24	38.323828296452675	40.0	37.0	41.0	34.0	41.0
25	38.23837112545677	40.0	37.0	41.0	34.0	41.0
26	38.20750537098072	40.0	37.0	41.0	34.0	41.0
27	38.170442893168286	40.0	37.0	41.0	34.0	41.0
28	38.152286456076766	40.0	37.0	41.0	34.0	41.0
29	38.11340277145906	40.0	37.0	41.0	34.0	41.0
30	38.05355375765927	40.0	37.0	41.0	34.0	41.0
31	37.84171482909273	40.0	36.0	41.0	33.0	41.0
32	37.879994116318684	40.0	36.0	41.0	34.0	41.0
33	37.88131417302455	40.0	36.0	41.0	34.0	41.0
34	37.83018931970406	40.0	36.0	41.0	33.0	41.0
35	37.78135995070531	40.0	36.0	41.0	33.0	41.0
36	37.79235602306516	40.0	36.0	41.0	33.0	41.0
37	37.76249445458321	40.0	36.0	41.0	33.0	41.0
38	37.76427275954554	40.0	36.0	41.0	33.0	41.0
39	37.72456686817961	40.0	36.0	41.0	33.0	41.0
40	37.654081026023505	40.0	36.0	41.0	33.0	41.0
41	37.59472797638607	40.0	36.0	41.0	33.0	41.0
42	37.60100248877834	40.0	36.0	41.0	33.0	41.0
43	37.59028598557084	40.0	36.0	41.0	33.0	41.0
44	37.567276454289974	40.0	36.0	41.0	33.0	41.0
45	37.55309433078075	40.0	35.0	41.0	33.0	41.0
46	37.45705030500382	39.0	35.0	41.0	33.0	41.0
47	37.40589197881686	39.0	35.0	41.0	33.0	41.0
48	37.38017774563544	39.0	35.0	41.0	33.0	41.0
49	37.375044610844924	39.0	35.0	41.0	33.0	41.0
50	37.304198110338824	39.0	35.0	41.0	33.0	41.0
51	36.67123044842798	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	12.0
10	11.0
11	21.0
12	22.0
13	18.0
14	27.0
15	60.0
16	77.0
17	126.0
18	183.0
19	347.0
20	591.0
21	1012.0
22	1649.0
23	2734.0
24	4116.0
25	6026.0
26	8583.0
27	10762.0
28	14048.0
29	18053.0
30	24489.0
31	33139.0
32	45699.0
33	70011.0
34	119243.0
35	165551.0
36	166747.0
37	269434.0
38	456560.0
39	701116.0
40	652.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.336999633684265	4.527841645997564	56.59596977258723	15.539188947730938
2	18.80981801603963	4.426763018234226	54.413444588969696	22.34997437675644
3	20.270932209902217	4.423462876469565	52.7810530375212	22.524551876107022
4	19.009382303036933	4.580408189820383	51.14800145771976	25.262208049422924
5	16.221611119780533	16.263711499721136	45.49410429673743	22.020573083760898
6	21.119870106420144	6.678025440321416	53.65563775003878	18.546466703219664
7	83.64930619233886	2.230660108499232	9.145635727523324	4.974397971638582
8	85.75460805866332	2.4720890510253777	6.839260937966292	4.93404195234501
9	80.79661650608334	4.955728598227069	8.84263556864507	5.405019327044521
10	39.053500483942216	28.80811608578671	17.39089849188236	14.74748493838871
11	27.57263729886225	25.539797116713288	25.915211814884675	20.972353769539787
12	24.129175091850016	22.477029834695898	31.489056965632795	21.904738107821288
13	23.055167526982196	24.588460535726156	33.0263572893767	19.330014647914947
14	20.373425184136124	26.836564250695737	31.477223600162368	21.312786965005767
15	18.873086448156425	25.5656796571247	34.67958687882493	20.881647015893954
16	20.640925246603093	25.647617462653006	31.15734557340199	22.554111717341915
17	20.84657122342384	25.20360696065901	29.20304876525196	24.74677305066519
18	21.21430130577181	24.731498108783047	31.367988907752082	22.68621167769307
19	22.652550231693525	26.59852973969896	29.738426049244715	21.0104939793628
20	23.940831286852564	27.392072399452932	29.63598021989316	19.03111609380134
21	23.78888333103109	26.820110686754788	29.394315552955252	19.99669042925887
22	21.806865332057907	25.406707113832734	29.099188589429836	23.687238964679523
23	20.84199816983567	26.632568344757324	28.54910210214316	23.976331383263847
24	21.793287605940446	24.853414774546103	29.60604321959945	23.74725439991401
25	20.85354866601198	26.44483742323045	27.909204613975348	24.79240929678222
26	19.300077647621233	27.396504018394047	28.476310403791206	24.827107930193513
27	19.88849292426033	26.20779295476307	30.82945291664172	23.074261204334878
28	17.920712679757543	25.0754200255431	32.97831665425971	24.025550640439654
29	20.593497494956676	23.432656599977086	30.937650421640257	25.03619548342598
30	22.68970039898714	24.443160008316358	30.682125159290774	22.18501443340573
31	22.15290876852381	26.639262918051347	28.7310342031407	22.47679411028414
32	22.182044305817534	26.671651452227387	27.066678421457336	24.079625820497746
33	22.24026823552263	25.987013470707232	27.852395030740823	23.920323263029314
34	20.071603647316678	24.454191910786797	29.931625777124456	25.54257866477207
35	19.51958421985356	23.89736370532374	29.13723450948814	27.445817565334558
36	20.983338527127874	25.896872455649632	29.41076911689621	23.70901990032629
37	19.87779103596636	26.294350958761896	32.55886863597126	21.268989369300478
38	21.917278646527002	26.434842708171765	28.67149021672974	22.976388428571497
39	23.647071524915365	25.116766087366067	28.624156754848023	22.612005632870545
40	22.734582326986533	23.36208071109569	29.93992327641846	23.96341368549932
41	18.41488533657439	25.382993238009526	28.55895538255479	27.6431660428613
42	19.010938084154557	25.936521301707916	28.026076777326708	27.026463836810816
43	20.496661906605045	24.83092666566405	29.51114057142426	25.16127085630664
44	20.95151573154007	23.61963320338632	29.162268442017215	26.266582623056394
45	22.716997285869123	22.27199674134573	27.76871286456548	27.242293108219663
46	22.313672817345168	22.958143359101154	29.956329695477063	24.77185412807662
47	19.034180511154243	23.80689267608967	32.09123854791876	25.06768826483732
48	19.231906147739803	23.097880790393383	32.32630293132735	25.343910130539467
49	22.072479599230785	21.600606471766582	32.273736387504535	24.053177541498105
50	22.205145298170166	21.49665200617975	30.104553205592705	26.193649490057382
51	20.217564203079412	21.11878577412604	28.679174832553162	29.98447519024139
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	404.0
1	1003.0
2	1602.0
3	2440.5
4	3279.0
5	2531.5
6	1784.0
7	1915.5
8	2047.0
9	2435.0
10	2823.0
11	2949.5
12	3076.0
13	3327.0
14	3578.0
15	3626.0
16	3674.0
17	3528.5
18	3383.0
19	4138.0
20	4893.0
21	5415.0
22	5937.0
23	7318.5
24	8700.0
25	10160.5
26	15406.5
27	19192.0
28	22935.0
29	26678.0
30	30540.0
31	34402.0
32	39281.0
33	44160.0
34	50534.0
35	56908.0
36	63462.5
37	70017.0
38	78255.5
39	86494.0
40	96556.0
41	106618.0
42	122155.5
43	137693.0
44	151508.5
45	165324.0
46	192432.5
47	219541.0
48	240035.0
49	260529.0
50	246240.0
51	231951.0
52	198182.5
53	164414.0
54	141063.0
55	117712.0
56	100898.5
57	84085.0
58	77198.0
59	70311.0
60	63367.5
61	56424.0
62	48171.5
63	39919.0
64	34057.5
65	28196.0
66	22586.0
67	16976.0
68	13667.5
69	10359.0
70	8559.0
71	6759.0
72	5443.5
73	4128.0
74	3393.5
75	1947.0
76	1235.0
77	1158.5
78	1082.0
79	709.5
80	337.0
81	251.5
82	166.0
83	98.5
84	31.0
85	21.0
86	11.0
87	8.5
88	6.0
89	3.5
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2121121.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.885585666890826
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.25878895534186	15.991175973988181
2	13.892308599663886	6.914364715356395
3	6.3615609517298655	4.749335101182633
4	3.5314779052175846	3.5153158376409746
5	2.1974472520485393	2.7342380919663887
6	1.4372377310650104	2.145990160806367
7	0.982251485175884	1.7110732436542642
8	0.73363696657995	1.4605588464178614
9	0.542671795223232	1.2154234904129857
>10	4.574809104437818	26.278390979264117
>50	1.3070676301470208	22.292751827330058
>100	0.1691269451749385	6.783443820335452
>500	0.0075393525121355935	1.260814482907653
>1k	0.0032602605457883642	1.2812499814843819
>5k	8.150651364470911E-4	1.6658734472523418
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGCT	9179	0.4327428751117923	No Hit
CCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	9069	0.4275569380530389	No Hit
TCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	8043	0.3791862887595757	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	6561	0.3093175731134622	No Hit
TCCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTG	3312	0.15614385035082864	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4143464705691E-4	0.0	0.0	0.44575486264102804	0.0
2	1.4143464705691E-4	0.0	0.0	1.6264512962721127	0.0
3	1.4143464705691E-4	0.0	0.0	2.3043947044982347	0.0
4	1.4143464705691E-4	0.0	0.0	3.054186913429267	0.0
5	1.4143464705691E-4	0.0	0.0	4.330587458235527	0.0
6	1.4143464705691E-4	0.0	0.0	5.141950883518668	0.0
7	1.4143464705691E-4	0.0	0.0	5.892261686155575	0.0
8	1.4143464705691E-4	0.0	0.0	7.10610097208033	0.0
9	1.8857952940921332E-4	0.0	0.0	7.606449608485325	0.0
10	1.8857952940921332E-4	0.0	0.0	8.705726830293981	0.0
11	2.3572441176151668E-4	0.0	0.0	10.982211764439652	0.0
12	2.3572441176151668E-4	0.0	0.0	13.113820475116695	0.0
13	2.3572441176151668E-4	0.0	0.0	13.757489553872693	0.0
14	2.3572441176151668E-4	0.0	0.0	13.954366582575911	0.0
15	2.3572441176151668E-4	0.0	0.0	14.246287694101374	0.0
16	2.3572441176151668E-4	0.0	0.0	15.006074618091095	0.0
17	2.8286929411382E-4	0.0	0.0	16.22241258278052	0.0
18	2.8286929411382E-4	0.0	0.0	17.5299287499393	0.0
19	2.8286929411382E-4	0.0	0.0	18.477823754514713	0.0
20	2.8286929411382E-4	0.0	0.0	19.38795570832593	0.0
21	3.3001417646612335E-4	0.0	0.0	20.725880324601945	0.0
22	3.3001417646612335E-4	0.0	0.0	22.138199565229893	0.0
23	3.3001417646612335E-4	0.0	0.0	23.476972789388252	0.0
24	3.3001417646612335E-4	0.0	0.0	24.483091723668757	0.0
25	3.3001417646612335E-4	0.0	0.0	25.391337882185884	0.0
26	3.3001417646612335E-4	0.0	0.0	26.178563127704642	0.0
27	3.7715905881842665E-4	0.0	0.0	26.94810904234129	0.0
28	3.7715905881842665E-4	0.0	0.0	27.781300548153546	0.0
29	3.7715905881842665E-4	0.0	0.0	28.6573938969064	0.0
30	3.7715905881842665E-4	0.0	0.0	29.598028589599558	0.0
31	3.7715905881842665E-4	0.0	0.0	30.463797209117253	0.0
32	4.2430394117073E-4	0.0	0.0	31.29001127234137	4.714488235230333E-5
33	4.7144882352303337E-4	0.0	0.0	32.115565307212556	9.428976470460666E-5
34	4.7144882352303337E-4	0.0	0.0	32.915095367025266	9.428976470460666E-5
35	5.185937058753367E-4	0.0	0.0	33.768323447837254	9.428976470460666E-5
36	5.185937058753367E-4	0.0	0.0	34.56478909029706	9.428976470460666E-5
37	5.185937058753367E-4	0.0	0.0	35.32443457963973	9.428976470460666E-5
38	5.185937058753367E-4	0.0	0.0	36.11495996692315	9.428976470460666E-5
39	5.185937058753367E-4	0.0	0.0	36.921656048853414	9.428976470460666E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGA	25	3.891925E-5	45.0	30
TAGCGAT	25	3.891925E-5	45.0	24
CACGCGT	25	3.891925E-5	45.0	16
CGCGACA	20	7.0346385E-4	45.0	29
CCGTCGT	25	3.891925E-5	45.0	34
CGGATCG	25	3.891925E-5	45.0	12
AACGCAC	25	3.891925E-5	45.0	23
TATCGGC	20	7.0346385E-4	45.0	24
ACGCGTG	25	3.891925E-5	45.0	17
TACGGGA	905	0.0	42.26519	4
GCGATAG	80	0.0	42.1875	9
TGCGACG	70	0.0	41.785713	1
CGAACGA	60	3.6379788E-12	41.249996	22
TCTACGG	475	0.0	41.210526	2
TTACGGG	1305	0.0	41.206894	3
CGTATGG	270	0.0	40.833332	2
TTCGTAC	45	1.929584E-8	40.0	33
TCTTGCG	790	0.0	39.873417	1
TTATGGG	4625	0.0	39.794594	3
GCACGGG	345	0.0	39.782608	3
>>END_MODULE