SRR2935278.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935278.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	727352
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	6551	0.900664327588293	TruSeq Adapter, Index 21 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	6377	0.8767419351290708	RNA PCR Primer, Index 21 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGCT	5995	0.8242226597300895	RNA PCR Primer, Index 21 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	4863	0.6685896237310134	TruSeq Adapter, Index 21 (96% over 25bp)
TCCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTG	2378	0.3269393636093666	RNA PCR Primer, Index 21 (96% over 25bp)
TTCCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCT	1759	0.2418361398607552	TruSeq Adapter, Index 21 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTG	1330	0.18285506879750107	TruSeq Adapter, Index 21 (95% over 24bp)
TGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTG	1261	0.17336860282229236	RNA PCR Primer, Index 14 (95% over 24bp)
TTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTG	1204	0.1655319570166852	RNA PCR Primer, Index 14 (95% over 24bp)
TGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGCTT	1190	0.16360716681881676	TruSeq Adapter, Index 21 (96% over 27bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCC	1157	0.1590701613524126	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTG	929	0.12772357812998383	RNA PCR Primer, Index 21 (96% over 25bp)
CGTTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTC	917	0.1260737579603823	RNA PCR Primer, Index 14 (95% over 22bp)
TTTCCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTC	897	0.12332405767771312	RNA PCR Primer, Index 14 (95% over 22bp)
TCTGACTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	847	0.11644980697104017	RNA PCR Primer, Index 21 (96% over 26bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGC	772	0.1061384309110307	No Hit
GTTCCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTC	758	0.10421364071316226	RNA PCR Primer, Index 21 (95% over 23bp)
TGCCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCT	756	0.10393867068489535	RNA PCR Primer, Index 21 (95% over 24bp)
GCTGACTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	743	0.10215136550116037	RNA PCR Primer, Index 21 (96% over 26bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCG	742	0.1020138804870269	No Hit
TTGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCT	731	0.10050154533155886	RNA PCR Primer, Index 21 (95% over 24bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATGGG	55	1.8189894E-12	45.000004	3
AGCTACG	55	1.8189894E-12	45.000004	9
AGCACCG	20	7.031577E-4	45.0	20
TCCGGAC	20	7.031577E-4	45.0	19
ACAACGG	35	1.2111013E-7	45.0	26
AACCGGC	20	7.031577E-4	45.0	31
AGGTAAT	20	7.031577E-4	45.0	30
CGGCGGA	20	7.031577E-4	45.0	20
TTGGCCG	20	7.031577E-4	45.0	1
CCCTCCG	20	7.031577E-4	45.0	16
GTACAGT	25	3.8893886E-5	45.0	29
GATCGGC	20	7.031577E-4	45.0	10
CGAGTCG	20	7.031577E-4	45.0	36
GGTCGAT	40	6.8084773E-9	45.0	15
TACCACG	25	3.8893886E-5	45.0	11
GCGGTGC	25	3.8893886E-5	45.0	9
CCCTATG	20	7.031577E-4	45.0	1
TAGACCA	25	3.8893886E-5	45.0	33
ACACCGA	20	7.031577E-4	45.0	43
GGCGTAA	20	7.031577E-4	45.0	8

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content