##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935278.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	727352
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14085064727945	33.0	31.0	34.0	30.0	34.0
2	32.291204258735796	34.0	31.0	34.0	30.0	34.0
3	32.35717644276774	34.0	31.0	34.0	30.0	34.0
4	35.86603460222836	37.0	35.0	37.0	35.0	37.0
5	31.57764328688173	37.0	35.0	37.0	0.0	37.0
6	33.36640993631694	37.0	35.0	37.0	17.0	37.0
7	35.16782520705243	37.0	35.0	37.0	32.0	37.0
8	35.71523278962593	37.0	35.0	37.0	35.0	37.0
9	37.73603977166489	39.0	37.0	39.0	35.0	39.0
10	37.4280155963	39.0	37.0	39.0	35.0	39.0
11	37.33570540811051	39.0	37.0	39.0	34.0	39.0
12	37.078410453260595	39.0	37.0	39.0	34.0	39.0
13	36.90356388653637	39.0	37.0	39.0	33.0	39.0
14	37.943419417283515	40.0	37.0	41.0	33.0	41.0
15	38.07988566746225	40.0	37.0	41.0	33.0	41.0
16	38.18222676228291	40.0	37.0	41.0	34.0	41.0
17	38.216569143963305	40.0	37.0	41.0	34.0	41.0
18	38.217659400125385	40.0	37.0	41.0	34.0	41.0
19	38.263923107381295	40.0	37.0	41.0	34.0	41.0
20	38.16435646014585	40.0	37.0	41.0	34.0	41.0
21	38.10714757091477	40.0	37.0	41.0	34.0	41.0
22	38.07004449015057	40.0	36.0	41.0	34.0	41.0
23	38.02750525192754	40.0	36.0	41.0	34.0	41.0
24	38.001634696818044	40.0	36.0	41.0	34.0	41.0
25	37.89995215521508	40.0	36.0	41.0	34.0	41.0
26	37.88252867937395	40.0	36.0	41.0	34.0	41.0
27	37.80878721719333	40.0	36.0	41.0	34.0	41.0
28	37.81939418603372	40.0	36.0	41.0	34.0	41.0
29	37.82561950747368	40.0	36.0	41.0	34.0	41.0
30	37.729279633519944	40.0	36.0	41.0	33.0	41.0
31	37.43307092026969	39.0	36.0	41.0	33.0	41.0
32	37.52980812591427	39.0	35.0	41.0	33.0	41.0
33	37.53468609421573	39.0	35.0	41.0	33.0	41.0
34	37.48838664085615	39.0	35.0	41.0	33.0	41.0
35	37.5154134449345	39.0	35.0	41.0	33.0	41.0
36	37.51837762183922	39.0	35.0	41.0	33.0	41.0
37	37.43214702097471	39.0	35.0	41.0	33.0	41.0
38	37.457250409705345	39.0	35.0	41.0	33.0	41.0
39	37.44661731871226	39.0	35.0	41.0	33.0	41.0
40	37.30792518615471	39.0	35.0	41.0	33.0	41.0
41	37.26870896072328	39.0	35.0	41.0	33.0	41.0
42	37.36502683707476	39.0	35.0	41.0	33.0	41.0
43	37.352963901934686	39.0	35.0	41.0	33.0	41.0
44	37.30957500632431	39.0	35.0	41.0	33.0	41.0
45	37.30317232921612	39.0	35.0	41.0	33.0	41.0
46	37.15988682233637	39.0	35.0	41.0	33.0	41.0
47	37.08986982918862	39.0	35.0	41.0	32.0	41.0
48	37.147855508749544	39.0	35.0	41.0	33.0	41.0
49	37.18505070447321	39.0	35.0	41.0	33.0	41.0
50	37.09599891111869	39.0	35.0	41.0	32.0	41.0
51	36.439473597377884	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	4.0
12	6.0
13	3.0
14	10.0
15	9.0
16	14.0
17	45.0
18	67.0
19	110.0
20	180.0
21	377.0
22	587.0
23	907.0
24	1370.0
25	2171.0
26	2926.0
27	3981.0
28	5313.0
29	7296.0
30	9525.0
31	13309.0
32	18739.0
33	28735.0
34	47339.0
35	67063.0
36	62043.0
37	95804.0
38	157298.0
39	201939.0
40	177.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.748407923536334	4.433754220789933	56.85239058942575	15.965447266247978
2	18.62550731970215	4.792315137649996	54.103515216841366	22.478662325806486
3	19.663656661423907	4.747495023042489	52.350718771653895	23.23812954387972
4	19.27388664635555	4.174045029091829	51.90512984084735	24.646938483705277
5	15.425131160703485	16.495864450774867	45.226107854243885	22.852896534277765
6	20.21744629835348	5.794993345725316	54.14819784643474	19.839362509486467
7	78.66493802175563	1.919015827274827	12.672268722709223	6.743777428260319
8	78.72213178763515	2.5124011482748383	10.102811293569001	8.662655770521013
9	73.41369790692815	4.960871764977617	13.337558706101035	8.287871621993203
10	36.585862141026624	25.644117291215256	20.581781585807146	17.188238981950967
11	27.389077090597127	25.42565360375719	27.203059866474554	19.982209439171132
12	24.59345681320736	20.65464864329788	33.579339851956135	21.172554691538622
13	21.41659059162551	27.648236342238697	32.79787503162155	18.137298034514238
14	16.64544814615207	30.0844707926836	31.27605341017829	21.99402765098604
15	14.105824965078806	26.21646740505285	38.57911987593352	21.098587753934822
16	14.953007622169185	26.255100694024353	34.02492878276268	24.766962901043787
17	15.60619892431725	25.591872985844542	29.2150155633036	29.586912526534608
18	17.438874162716264	24.275316490502536	34.049263630264306	24.2365457165169
19	19.681942168303653	25.103113760600092	31.391128367007997	23.823815704088254
20	21.71644540745059	26.766407461586688	30.736149759676195	20.78099737128653
21	19.804303830882432	29.003151156523938	30.53377181887174	20.658773193721885
22	17.48768134273364	26.610911910601743	28.425164157106874	27.476242589557735
23	16.97362487488864	27.197972920951617	28.369070271340423	27.459331932819325
24	20.08353589458749	24.48539359209846	29.860232734631925	25.570837778682126
25	16.12053036219052	27.208559267039895	28.331399377467857	28.33951099330173
26	16.28661225926374	27.16951352302599	29.221202388939606	27.322671828770662
27	17.834831003420625	26.58066520749238	31.620453370582606	23.96405041850438
28	13.812431944918005	23.873310309176297	35.76631397177707	26.54794377412862
29	15.783417107535277	21.81625952771148	32.4142918421892	29.986031522564037
30	20.00998141202609	24.577508551567878	31.325548015266335	24.086962021139698
31	19.542394878958195	27.83961548191247	28.023020490766505	24.59496914836283
32	19.325718496683862	26.0506604780079	27.127168138672886	27.49645288663536
33	20.237656595431098	24.376230490876495	27.60314125760292	27.782971656089483
34	16.78375807037033	23.40187419571267	31.301488137792983	28.512879596124023
35	15.469401335254457	21.676987208394287	29.962246615118953	32.89136484123231
36	19.730886833335166	22.65285583871358	30.926841474279303	26.68941585367195
37	16.18611071393218	24.652162914242346	35.87217743265984	23.28954893916563
38	17.029718760655086	25.78091488027805	30.80118017136132	26.388186187705543
39	20.72806564084515	23.308384386101917	29.09430372089442	26.86924625215851
40	22.463813944280073	23.591328545188574	29.752306998537158	24.19255051199419
41	16.475654153697246	24.315737084657773	28.90333703626305	30.30527172538193
42	17.798259989661126	20.123406548686194	31.307674963428987	30.770658498223696
43	20.00970644199782	19.91539172230227	31.235357845994788	28.839543989705124
44	17.86961471199639	21.249821269481625	30.215631496166917	30.664932522355066
45	19.31444472552492	21.354997305293725	26.981296538677285	32.34926143050407
46	21.631204698687842	21.989490645519638	31.70968114475522	24.669623511037297
47	15.473663370692595	22.947486224001583	35.57878441249904	26.000065992806785
48	16.359616801768606	20.135367744915804	34.58243051507386	28.92258493824173
49	19.697203004872467	17.67837305733675	35.41366491052475	27.21075902726603
50	19.835787899118994	18.859506813757303	31.729616471804572	29.575088815319127
51	17.075776240389796	19.51476039111737	28.736430229105025	34.673033139387805
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	270.0
1	322.5
2	375.0
3	693.0
4	1011.0
5	735.5
6	460.0
7	601.5
8	743.0
9	1070.0
10	1397.0
11	1679.0
12	1961.0
13	1881.0
14	1801.0
15	1782.0
16	1763.0
17	1639.0
18	1515.0
19	1619.0
20	1723.0
21	1904.0
22	2085.0
23	2322.5
24	2560.0
25	2905.5
26	4045.5
27	4840.0
28	5736.5
29	6633.0
30	7852.5
31	9072.0
32	10950.5
33	12829.0
34	14169.5
35	15510.0
36	17275.5
37	19041.0
38	21945.0
39	24849.0
40	30102.0
41	35355.0
42	41947.5
43	48540.0
44	57103.5
45	65667.0
46	81138.0
47	96609.0
48	107499.0
49	118389.0
50	106715.0
51	95041.0
52	74467.0
53	53893.0
54	42819.0
55	31745.0
56	26275.0
57	20805.0
58	17637.5
59	14470.0
60	12939.0
61	11408.0
62	9955.0
63	8502.0
64	6865.0
65	5228.0
66	4076.5
67	2925.0
68	2410.5
69	1896.0
70	1621.0
71	1346.0
72	1093.5
73	841.0
74	680.5
75	364.5
76	209.0
77	189.0
78	169.0
79	117.5
80	66.0
81	45.0
82	24.0
83	17.5
84	11.0
85	7.0
86	3.0
87	2.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	727352.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.94223169023508
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.81196618506678	17.830487437525825
2	13.716868689253156	7.665598459592858
3	6.3844003308326	5.35183179742014
4	3.695163858461971	4.130044986661097
5	2.447601546405321	3.419572474751758
6	1.6396332225566774	2.7489006835011276
7	1.2279938081838966	2.401902125171398
8	0.8672102988645491	1.9385432876024997
9	0.6853152255342675	1.723431313145179
>10	4.786896811579744	28.813487562684788
>50	0.5984957217642161	10.857939182967721
>100	0.12176122968517349	6.293395775512643
>500	0.011292372107899153	2.15153084421164
>1k	0.0039277816027475316	2.0773117415706595
>5k	0.0014729181010303244	2.596022327680729
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	6551	0.900664327588293	TruSeq Adapter, Index 21 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	6377	0.8767419351290708	RNA PCR Primer, Index 21 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGCT	5995	0.8242226597300895	RNA PCR Primer, Index 21 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	4863	0.6685896237310134	TruSeq Adapter, Index 21 (96% over 25bp)
TCCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTG	2378	0.3269393636093666	RNA PCR Primer, Index 21 (96% over 25bp)
TTCCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCT	1759	0.2418361398607552	TruSeq Adapter, Index 21 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTG	1330	0.18285506879750107	TruSeq Adapter, Index 21 (95% over 24bp)
TGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTG	1261	0.17336860282229236	RNA PCR Primer, Index 14 (95% over 24bp)
TTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTG	1204	0.1655319570166852	RNA PCR Primer, Index 14 (95% over 24bp)
TGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGCTT	1190	0.16360716681881676	TruSeq Adapter, Index 21 (96% over 27bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCC	1157	0.1590701613524126	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTG	929	0.12772357812998383	RNA PCR Primer, Index 21 (96% over 25bp)
CGTTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTC	917	0.1260737579603823	RNA PCR Primer, Index 14 (95% over 22bp)
TTTCCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTC	897	0.12332405767771312	RNA PCR Primer, Index 14 (95% over 22bp)
TCTGACTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	847	0.11644980697104017	RNA PCR Primer, Index 21 (96% over 26bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGC	772	0.1061384309110307	No Hit
GTTCCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTC	758	0.10421364071316226	RNA PCR Primer, Index 21 (95% over 23bp)
TGCCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCT	756	0.10393867068489535	RNA PCR Primer, Index 21 (95% over 24bp)
GCTGACTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	743	0.10215136550116037	RNA PCR Primer, Index 21 (96% over 26bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCG	742	0.1020138804870269	No Hit
TTGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCT	731	0.10050154533155886	RNA PCR Primer, Index 21 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3748501413345945E-4	0.0	0.0	0.8585939132634542	0.0
2	1.3748501413345945E-4	0.0	0.0	3.482495408000528	0.0
3	1.3748501413345945E-4	0.0	0.0	4.888417162529285	0.0
4	1.3748501413345945E-4	0.0	0.0	6.472794465403271	0.0
5	1.3748501413345945E-4	0.0	0.0	9.134779309055313	0.0
6	1.3748501413345945E-4	0.0	0.0	10.766588941805344	0.0
7	1.3748501413345945E-4	0.0	0.0	12.247165059008568	0.0
8	1.3748501413345945E-4	0.0	0.0	14.576298683443504	0.0
9	1.3748501413345945E-4	0.0	0.0	15.619122515645795	0.0
10	1.3748501413345945E-4	0.0	0.0	17.622141686556166	0.0
11	1.3748501413345945E-4	0.0	0.0	21.198264389181578	0.0
12	1.3748501413345945E-4	0.0	0.0	24.398778033194382	0.0
13	1.3748501413345945E-4	0.0	0.0	25.374234208471275	0.0
14	1.3748501413345945E-4	0.0	0.0	25.69746147669904	0.0
15	1.3748501413345945E-4	0.0	0.0	26.189932797325092	0.0
16	1.3748501413345945E-4	0.0	0.0	27.51322605835964	0.0
17	1.3748501413345945E-4	0.0	0.0	29.367073988935207	0.0
18	2.749700282669189E-4	0.0	0.0	31.63846390743409	0.0
19	2.749700282669189E-4	0.0	0.0	32.98554207591373	0.0
20	2.749700282669189E-4	0.0	0.0	34.3019610862416	0.0
21	2.749700282669189E-4	0.0	0.0	36.144122790615825	0.0
22	2.749700282669189E-4	0.0	0.0	37.80824140168722	0.0
23	2.749700282669189E-4	0.0	0.0	39.39578085988627	0.0
24	2.749700282669189E-4	0.0	0.0	40.60413664910525	0.0
25	4.1245504240037834E-4	0.0	0.0	41.6028277917707	0.0
26	4.1245504240037834E-4	0.0	0.0	42.506791759698196	0.0
27	4.1245504240037834E-4	0.0	0.0	43.359198847325644	0.0
28	4.1245504240037834E-4	0.0	0.0	44.276911316666485	0.0
29	4.1245504240037834E-4	0.0	0.0	45.25016773171724	0.0
30	4.1245504240037834E-4	0.0	0.0	46.36572113639613	0.0
31	4.1245504240037834E-4	0.0	0.0	47.3837976660544	0.0
32	4.1245504240037834E-4	0.0	0.0	48.21118248110956	0.0
33	4.1245504240037834E-4	0.0	0.0	49.078162980235156	0.0
34	4.1245504240037834E-4	0.0	0.0	49.91173462092632	0.0
35	4.1245504240037834E-4	0.0	0.0	50.79988781222847	0.0
36	4.1245504240037834E-4	0.0	0.0	51.638271428414306	0.0
37	4.1245504240037834E-4	0.0	0.0	52.40103828682673	0.0
38	4.1245504240037834E-4	0.0	0.0	53.18896490282559	0.0
39	4.1245504240037834E-4	0.0	0.0	54.00150133635434	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATGGG	55	1.8189894E-12	45.000004	3
AGCTACG	55	1.8189894E-12	45.000004	9
AGCACCG	20	7.031577E-4	45.0	20
TCCGGAC	20	7.031577E-4	45.0	19
ACAACGG	35	1.2111013E-7	45.0	26
AACCGGC	20	7.031577E-4	45.0	31
AGGTAAT	20	7.031577E-4	45.0	30
CGGCGGA	20	7.031577E-4	45.0	20
TTGGCCG	20	7.031577E-4	45.0	1
CCCTCCG	20	7.031577E-4	45.0	16
GTACAGT	25	3.8893886E-5	45.0	29
GATCGGC	20	7.031577E-4	45.0	10
CGAGTCG	20	7.031577E-4	45.0	36
GGTCGAT	40	6.8084773E-9	45.0	15
TACCACG	25	3.8893886E-5	45.0	11
GCGGTGC	25	3.8893886E-5	45.0	9
CCCTATG	20	7.031577E-4	45.0	1
TAGACCA	25	3.8893886E-5	45.0	33
ACACCGA	20	7.031577E-4	45.0	43
GGCGTAA	20	7.031577E-4	45.0	8
>>END_MODULE