##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935275.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1297000
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32767694680031	33.0	31.0	34.0	30.0	34.0
2	32.47580107941403	34.0	31.0	34.0	30.0	34.0
3	32.53764687740941	34.0	31.0	34.0	30.0	34.0
4	35.9814633770239	37.0	35.0	37.0	35.0	37.0
5	31.739302235929067	37.0	35.0	37.0	0.0	37.0
6	33.398531225905934	37.0	35.0	37.0	17.0	37.0
7	35.22678720123361	37.0	35.0	37.0	32.0	37.0
8	35.73999460292984	37.0	35.0	37.0	35.0	37.0
9	37.803257517347724	39.0	38.0	39.0	35.0	39.0
10	37.546866615266	39.0	37.0	39.0	35.0	39.0
11	37.44005859676176	39.0	37.0	39.0	35.0	39.0
12	37.16833616037008	39.0	37.0	39.0	34.0	39.0
13	37.058104086353126	39.0	37.0	39.0	33.0	39.0
14	38.235141865844255	40.0	37.0	41.0	33.0	41.0
15	38.33063454124903	40.0	37.0	41.0	34.0	41.0
16	38.44212027756361	40.0	37.0	41.0	34.0	41.0
17	38.435915959907476	40.0	37.0	41.0	34.0	41.0
18	38.40839784117193	40.0	37.0	41.0	34.0	41.0
19	38.415555127216656	40.0	37.0	41.0	34.0	41.0
20	38.311707016191214	40.0	37.0	41.0	34.0	41.0
21	38.27857054741712	40.0	37.0	41.0	34.0	41.0
22	38.226372397841175	40.0	37.0	41.0	34.0	41.0
23	38.15337856592136	40.0	37.0	41.0	34.0	41.0
24	38.14094834232845	40.0	37.0	41.0	34.0	41.0
25	38.045124903623744	40.0	36.0	41.0	34.0	41.0
26	37.99973168851195	40.0	36.0	41.0	34.0	41.0
27	37.918879722436394	40.0	36.0	41.0	34.0	41.0
28	37.88412104857363	40.0	36.0	41.0	34.0	41.0
29	37.86143793369314	40.0	36.0	41.0	34.0	41.0
30	37.8118966846569	40.0	36.0	41.0	33.0	41.0
31	37.63216345412491	40.0	36.0	41.0	33.0	41.0
32	37.62830840400925	40.0	36.0	41.0	33.0	41.0
33	37.56519352351581	40.0	35.0	41.0	33.0	41.0
34	37.544257517347724	40.0	35.0	41.0	33.0	41.0
35	37.46913030069391	40.0	35.0	41.0	33.0	41.0
36	37.47686815728604	40.0	35.0	41.0	33.0	41.0
37	37.48384579799537	40.0	35.0	41.0	33.0	41.0
38	37.44602698535081	40.0	35.0	41.0	33.0	41.0
39	37.418098689282964	39.0	35.0	41.0	33.0	41.0
40	37.30833616037008	39.0	35.0	41.0	33.0	41.0
41	37.23054818812645	39.0	35.0	41.0	33.0	41.0
42	37.22619198149576	39.0	35.0	41.0	33.0	41.0
43	37.192336931380105	39.0	35.0	41.0	33.0	41.0
44	37.16801233616037	39.0	35.0	41.0	33.0	41.0
45	37.13667771781033	39.0	35.0	41.0	33.0	41.0
46	37.03989976869699	39.0	35.0	41.0	33.0	41.0
47	36.97919814957594	39.0	35.0	41.0	32.0	41.0
48	36.97006630686199	39.0	35.0	41.0	33.0	41.0
49	36.95384579799537	39.0	35.0	41.0	33.0	41.0
50	36.88375019275251	39.0	35.0	41.0	32.0	41.0
51	36.250084811102546	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	13.0
11	13.0
12	12.0
13	25.0
14	11.0
15	27.0
16	37.0
17	69.0
18	167.0
19	303.0
20	529.0
21	922.0
22	1600.0
23	2558.0
24	3629.0
25	4713.0
26	6329.0
27	7621.0
28	9574.0
29	12353.0
30	16351.0
31	21978.0
32	30631.0
33	46446.0
34	79440.0
35	117812.0
36	104948.0
37	165363.0
38	266623.0
39	396446.0
40	450.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.698920585967617	4.304703161141095	52.57733230531997	17.419043947571318
2	19.40362374710871	7.028296067848883	52.34009252120278	21.227987663839627
3	20.548882035466463	7.0863531225905945	49.730377794911334	22.634387047031613
4	19.005088666152663	4.539861218195837	51.58519660755589	24.869853508095606
5	17.47447956823439	16.943716268311487	42.79776407093292	22.784040092521202
6	21.2798766383963	8.594448727833463	51.870778720123354	18.254895913646877
7	82.01873554356207	2.566615265998458	10.544872783346184	4.869776407093292
8	83.01858134155744	4.832228218966846	6.759059367771782	5.390131071703933
9	78.43955281418658	5.220740169622205	8.530840400925213	7.808866615265998
10	41.65566692367001	27.620200462606014	15.75805705474171	14.966075558982267
11	31.929915188897457	24.08542791056284	24.12112567463377	19.863531225905938
12	28.187047031611414	21.52220508866615	29.542713955281418	20.748033924441017
13	24.557363145720895	24.490593677717808	30.692058596761758	20.259984579799536
14	19.986892829606784	28.593986121819583	28.978797224363916	22.440323824209717
15	17.50794140323824	24.649653045489593	36.19059367771781	21.651811873554355
16	19.321202775636085	23.748882035466462	32.73685427910563	24.19306090979183
17	20.42706245181187	23.394217424826522	29.461680801850427	26.717039321511184
18	20.74656900539707	24.08982266769468	30.468234387047033	24.695373939861216
19	23.098072474942175	25.368234387047035	29.05165767154973	22.482035466461063
20	26.599614494988433	25.059290670778722	28.863299922899	19.47779491133385
21	25.126291441788744	26.492289899768696	27.378797224363915	21.002621434078645
22	22.88010794140324	24.07509637625289	27.610485736314573	25.4343099460293
23	21.377101002313033	26.254433307633	27.145643793369313	25.222821896684657
24	22.073554356206632	23.478180416345413	29.99814957594449	24.45011565150347
25	20.663222821896685	26.212952968388585	27.89691595990748	25.226908249807245
26	20.444333076329993	26.97270624518119	27.654973014649194	24.92798766383963
27	21.2727833461835	27.0555898226677	27.250192752505782	24.421434078643024
28	18.446954510408638	25.914109483423285	30.947031611410946	24.691904394757135
29	19.96515034695451	23.31202775636083	29.13893600616808	27.583885890516573
30	20.62359290670779	26.313415574402466	27.90107941403238	25.161912104857365
31	22.68141865844256	25.224286815728604	27.32636854279106	24.76792598303778
32	24.317347725520435	26.296299151888974	24.84579799537394	24.540555127216653
33	21.983346183500384	26.97224363916731	25.785119506553585	25.25929067077872
34	20.79244410177332	25.048727833461836	28.374016962220512	25.78481110254433
35	21.075404780262144	23.847802621434077	27.040400925212026	28.03639167309175
36	21.526676946800308	27.121202775636082	26.102004626060136	25.250115651503467
37	20.155435620663066	26.323361603700846	29.01557440246723	24.50562837316885
38	20.5017733230532	26.967077872012336	27.155050115651502	25.376098689282962
39	20.74965304548959	26.585890516576715	27.6159599074788	25.048496530454894
40	22.019737856592137	25.01703932151118	29.245104086353123	23.718118735543563
41	20.059984579799536	25.306553585196607	28.624826522744794	26.00863531225906
42	22.45728604471858	24.439244410177334	27.046183500385506	26.05728604471858
43	21.485042405551273	25.642713955281422	26.419198149575944	26.453045489591364
44	20.162220508866614	23.763916730917504	29.031380107941402	27.04248265227448
45	20.524595219737858	23.186430223592907	27.0686198920586	29.220354664610642
46	21.893369313801077	24.87756360832691	27.96206630686199	25.26700077101002
47	18.85412490362375	24.957286044718582	31.195605242868158	24.992983808789514
48	20.135543562066307	23.486353122590593	29.52513492675405	26.852968388589048
49	20.672552043176562	22.77124132613724	31.401619121048576	25.154587509637626
50	20.68619892058597	23.357594448727834	28.762683114880495	27.19352351580571
51	19.141711642251348	23.70794140323824	27.108558211256746	30.04178874325366
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	275.0
1	712.0
2	1149.0
3	1762.5
4	2376.0
5	1786.5
6	1197.0
7	1222.0
8	1247.0
9	1339.0
10	1431.0
11	1550.0
12	1669.0
13	1542.0
14	1415.0
15	1752.0
16	2089.0
17	2068.5
18	2048.0
19	2405.0
20	2762.0
21	3200.5
22	3639.0
23	4345.0
24	5051.0
25	5551.0
26	7896.5
27	9742.0
28	12674.0
29	15606.0
30	18467.5
31	21329.0
32	24077.5
33	26826.0
34	30188.5
35	33551.0
36	35372.0
37	37193.0
38	41801.5
39	46410.0
40	54162.5
41	61915.0
42	68456.0
43	74997.0
44	86044.5
45	97092.0
46	116346.0
47	135600.0
48	137860.5
49	140121.0
50	132046.0
51	123971.0
52	106807.5
53	89644.0
54	80323.5
55	71003.0
56	66622.0
57	62241.0
58	58659.0
59	55077.0
60	51291.0
61	47505.0
62	42699.0
63	37893.0
64	32256.0
65	26619.0
66	22344.5
67	18070.0
68	14379.5
69	10689.0
70	9125.5
71	7562.0
72	6663.5
73	5765.0
74	4953.5
75	3133.5
76	2125.0
77	1518.5
78	912.0
79	752.5
80	593.0
81	363.5
82	134.0
83	163.0
84	192.0
85	132.5
86	73.0
87	39.0
88	5.0
89	3.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1297000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.707637399752585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.64639706672244	16.03736253057543
2	12.312649157606916	5.838076433178277
3	5.389343219028877	3.8330578457865596
4	2.966874131807485	2.813503045103905
5	1.8838609962323893	2.2330946705107078
6	1.312022617140422	1.8662973880463722
7	0.9771023572716111	1.6215351872047201
8	0.7243609049759405	1.3738308545380988
9	0.6039609472491307	1.2886638426894141
>10	4.287865175906122	21.82155049379737
>50	1.573004935600499	26.677041698552628
>100	0.31212181533745925	10.33093018387591
>500	0.005870629755398457	0.9864681332469445
>1k	0.003913753170265638	2.382740397722044
>5k	6.52292195044273E-4	0.8958472951715862
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGC	6217	0.4793369313801079	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCG	5369	0.4139552814186585	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTC	4788	0.36915959907478796	No Hit
CCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCTGC	4615	0.35582112567463375	No Hit
GCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCTGC	4355	0.33577486507324594	No Hit
TCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCTGC	3945	0.3041634541249036	No Hit
CTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCTGCT	3748	0.2889745566692367	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCTCATGAT	1774	0.13677717810331533	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	1640	0.1264456437933693	No Hit
TCCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCTG	1517	0.11696222050886662	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5420200462606013E-4	0.0	0.0	0.29876638396299154	0.0
2	1.5420200462606013E-4	0.0	0.0	1.3582883577486506	0.0
3	1.5420200462606013E-4	0.0	0.0	1.8841942945258288	0.0
4	1.5420200462606013E-4	0.0	0.0	2.5655358519660756	0.0
5	1.5420200462606013E-4	0.0	0.0	3.9565921356977642	0.0
6	1.5420200462606013E-4	0.0	0.0	4.670316114109483	0.0
7	1.5420200462606013E-4	0.0	0.0	5.423207401696222	0.0
8	1.5420200462606013E-4	0.0	0.0	6.548882035466461	0.0
9	1.5420200462606013E-4	0.0	0.0	7.045335389360062	0.0
10	1.5420200462606013E-4	0.0	0.0	8.44402467232074	0.0
11	1.5420200462606013E-4	0.0	0.0	10.195836545875096	0.0
12	1.5420200462606013E-4	0.0	0.0	12.373939861218195	0.0
13	1.5420200462606013E-4	0.0	0.0	12.88496530454896	0.0
14	1.5420200462606013E-4	0.0	0.0	13.066229760986893	0.0
15	1.5420200462606013E-4	0.0	0.0	13.40763299922899	0.0
16	1.5420200462606013E-4	0.0	0.0	14.056283731688511	0.0
17	1.5420200462606013E-4	0.0	0.0	14.99414032382421	0.0
18	1.5420200462606013E-4	0.0	0.0	16.006707787201233	0.0
19	1.5420200462606013E-4	0.0	0.0	16.830609097918273	0.0
20	1.5420200462606013E-4	0.0	0.0	17.524286815728605	0.0
21	1.5420200462606013E-4	0.0	0.0	18.49992289899769	0.0
22	1.5420200462606013E-4	0.0	0.0	19.512336160370086	0.0
23	1.5420200462606013E-4	0.0	0.0	20.46538164996145	0.0
24	1.5420200462606013E-4	0.0	0.0	21.25520431765613	0.0
25	1.5420200462606013E-4	0.0	0.0	21.924672320740168	0.0
26	1.5420200462606013E-4	0.0	0.0	22.48550501156515	0.0
27	1.5420200462606013E-4	0.0	0.0	23.026676946800308	0.0
28	1.5420200462606013E-4	0.0	0.0	23.58218966846569	0.0
29	1.5420200462606013E-4	0.0	0.0	24.231457208943716	0.0
30	1.5420200462606013E-4	0.0	0.0	24.93816499614495	0.0
31	1.5420200462606013E-4	0.0	0.0	25.6035466461064	0.0
32	2.313030069390902E-4	0.0	0.0	26.194294525828838	0.0
33	2.313030069390902E-4	0.0	0.0	26.785813415574403	0.0
34	2.313030069390902E-4	0.0	0.0	27.365304548959138	0.0
35	2.313030069390902E-4	0.0	0.0	28.025828835774867	0.0
36	2.313030069390902E-4	0.0	0.0	28.64710871241326	0.0
37	3.0840400925212025E-4	0.0	0.0	29.243793369313803	0.0
38	3.0840400925212025E-4	0.0	0.0	29.85898226676947	0.0
39	3.0840400925212025E-4	0.0	0.0	30.501079414032382	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	30	2.165718E-6	45.000004	1
GTCGCTA	20	7.0336217E-4	45.000004	37
GTAATCG	20	7.0336217E-4	45.000004	30
CCCGTAT	20	7.0336217E-4	45.000004	22
CCCCGTA	20	7.0336217E-4	45.000004	21
CGCTATA	20	7.0336217E-4	45.000004	10
ACTCGTT	20	7.0336217E-4	45.000004	30
CGTCGTA	20	7.0336217E-4	45.000004	26
AATTACG	20	7.0336217E-4	45.000004	33
CGTAAGT	20	7.0336217E-4	45.000004	44
CTCGACA	20	7.0336217E-4	45.000004	31
CGTCCGA	20	7.0336217E-4	45.000004	35
GCTCGAT	20	7.0336217E-4	45.000004	28
CAATCGA	20	7.0336217E-4	45.000004	26
CGATCGA	60	0.0	45.000004	41
CGTCAGT	30	2.165718E-6	45.000004	19
TATCGAG	20	7.0336217E-4	45.000004	32
GTGCGAC	20	7.0336217E-4	45.000004	11
CGCCAAT	30	2.165718E-6	45.000004	20
GCGAGTA	20	7.0336217E-4	45.000004	39
>>END_MODULE