FastQCFastQC Report
Sat 14 Jan 2017
SRR2935273.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935273.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1357336
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC165681.2206262856065115TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGCT118430.8725179321848091TruSeq Adapter, Index 21 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGC98420.7250968072754277No Hit
TCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC93510.6889230080098074TruSeq Adapter, Index 15 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC82840.6103131428032558TruSeq Adapter, Index 15 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTC70380.5185156807157549No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCG59630.4393164257044681No Hit
TCCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTG36040.26552010703318857TruSeq Adapter, Index 21 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACAGGTAT35620.2624258105583289No Hit
CGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTG28350.20886501205302152TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCC22260.16399771316755762No Hit
TTCCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCT22090.1627452598324954No Hit
CGTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTC20720.15265195942640583No Hit
TGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTG19800.14587397667195154TruSeq Adapter, Index 21 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTG18040.13290740096777806No Hit
CCTGACTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC17990.1325390323398186TruSeq Adapter, Index 15 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCT17660.13010779939528608No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTC17110.12605574448773185No Hit
TTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTG16440.12111960487307491TruSeq Adapter, Index 21 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCT15530.11441529584421248No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACAGGTATCGTA14350.1057217962243689No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCTA253.8911792E-545.00000412
ACTAGTG253.8911792E-545.00000445
GCGATCG351.2121382E-745.0000049
ACGTAAC502.1827873E-1145.00000415
CGTCCAA253.8911792E-545.00000411
GCGAGAC207.0337387E-445.021
AGCGTCA207.0337387E-445.011
CTGTACG453.8562575E-1045.01
AGCCCGC207.0337387E-445.032
CAGTTCG207.0337387E-445.01
CATTGCG207.0337387E-445.01
ACCGAGC207.0337387E-445.012
CGACATA207.0337387E-445.010
CCATGCG207.0337387E-445.021
CGTTACC207.0337387E-445.017
CGCTAAA207.0337387E-445.019
GTCACGG207.0337387E-445.02
TTGCGGA551.8189894E-1245.03
AGCGATA207.0337387E-445.024
ACGTCGG207.0337387E-445.024
Produced by FastQC (version 0.11.5)