##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935273.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1357336
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19417299769549	33.0	31.0	34.0	30.0	34.0
2	32.3498065327966	34.0	31.0	34.0	30.0	34.0
3	32.415194911208424	34.0	31.0	34.0	30.0	34.0
4	35.914850854909915	37.0	35.0	37.0	35.0	37.0
5	31.859764273547597	37.0	35.0	37.0	0.0	37.0
6	33.44645393623981	37.0	35.0	37.0	17.0	37.0
7	35.23431118013521	37.0	35.0	37.0	32.0	37.0
8	35.72183453470622	37.0	35.0	37.0	35.0	37.0
9	37.81963935237848	39.0	38.0	39.0	35.0	39.0
10	37.47612455574743	39.0	37.0	39.0	35.0	39.0
11	37.38735582051902	39.0	37.0	39.0	35.0	39.0
12	37.27459892023788	39.0	37.0	39.0	34.0	39.0
13	37.22911423553195	39.0	37.0	39.0	34.0	39.0
14	38.39019152221705	40.0	38.0	41.0	34.0	41.0
15	38.42054509716091	40.0	38.0	41.0	34.0	41.0
16	38.50330058290652	40.0	38.0	41.0	34.0	41.0
17	38.48133181467227	40.0	38.0	41.0	34.0	41.0
18	38.488195995685665	40.0	38.0	41.0	34.0	41.0
19	38.53209816876588	40.0	38.0	41.0	34.0	41.0
20	38.426295331443356	40.0	38.0	41.0	34.0	41.0
21	38.397068227763796	40.0	38.0	41.0	34.0	41.0
22	38.377568266074135	40.0	38.0	41.0	34.0	41.0
23	38.318051683591975	40.0	37.0	41.0	34.0	41.0
24	38.271943719167545	40.0	37.0	41.0	34.0	41.0
25	38.18462414612152	40.0	37.0	41.0	34.0	41.0
26	38.156991341863765	40.0	37.0	41.0	34.0	41.0
27	38.030682896497254	40.0	37.0	41.0	34.0	41.0
28	38.038605768947406	40.0	37.0	41.0	34.0	41.0
29	37.99325369694755	40.0	37.0	41.0	34.0	41.0
30	37.91824500344793	40.0	37.0	41.0	33.0	41.0
31	37.721942098345586	40.0	37.0	41.0	33.0	41.0
32	37.53948690670549	40.0	36.0	41.0	33.0	41.0
33	37.557971644456494	39.0	36.0	41.0	33.0	41.0
34	37.591022414494276	40.0	36.0	41.0	33.0	41.0
35	37.39608910395068	39.0	36.0	41.0	32.0	41.0
36	37.442498393912786	39.0	36.0	41.0	33.0	41.0
37	37.395938072813216	39.0	36.0	41.0	33.0	41.0
38	37.409544873192786	39.0	36.0	41.0	33.0	41.0
39	37.49652996752462	39.0	36.0	41.0	33.0	41.0
40	37.41302521998974	39.0	36.0	41.0	33.0	41.0
41	37.19918723145927	39.0	35.0	41.0	32.0	41.0
42	37.12979763301055	39.0	35.0	41.0	32.0	41.0
43	37.21351750782415	39.0	35.0	41.0	32.0	41.0
44	37.2715517749474	39.0	36.0	41.0	32.0	41.0
45	37.312923992290784	39.0	36.0	41.0	32.0	41.0
46	37.16056304408046	39.0	35.0	41.0	32.0	41.0
47	37.01943660228565	39.0	35.0	41.0	31.0	41.0
48	36.99242044711111	39.0	35.0	41.0	31.0	41.0
49	37.00467238767704	39.0	35.0	41.0	31.0	41.0
50	36.93161162748207	39.0	35.0	41.0	31.0	41.0
51	36.181618994854624	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	2.0
11	6.0
12	4.0
13	5.0
14	27.0
15	21.0
16	33.0
17	52.0
18	94.0
19	197.0
20	434.0
21	793.0
22	1371.0
23	2112.0
24	3093.0
25	4312.0
26	5736.0
27	7126.0
28	9356.0
29	12771.0
30	17358.0
31	23959.0
32	32508.0
33	47098.0
34	73725.0
35	106894.0
36	121848.0
37	196458.0
38	317466.0
39	372160.0
40	310.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.167044858458038	4.229240217602716	56.33527733737262	15.268437586566627
2	17.794341268484736	7.4244696965231896	53.610380922630796	21.17080811236127
3	18.289502378187862	7.170442690682337	53.578332851998326	20.961722079131476
4	18.590017504877203	3.729511336912894	55.82125575391797	21.859215404291938
5	17.013105082308286	15.540662002628677	46.21044457672971	21.23578833833332
6	18.248760807935543	8.067714994666023	54.21428445130756	19.46923974609087
7	77.34989715147907	1.3576594152074357	14.76067826978729	6.5317651635262015
8	79.18953007950869	3.910822375594547	9.179304166396529	7.720343378500239
9	75.15839851002258	3.2850377504169934	12.301154614627476	9.255409124932958
10	34.51481431274202	25.9916483464669	21.022429229019195	18.471108111771883
11	24.41981941096383	27.671114595059738	27.81477835996393	20.094287634012506
12	21.026997000005892	23.131707992715143	33.53841642747264	22.302878579806325
13	20.792493531446894	24.738237252972	35.595313172272746	18.87395604330836
14	17.484322231194042	28.89144618576388	30.957920514890937	22.666311068151142
15	14.05149498723971	27.565982188640103	36.27274307909022	22.10977974502997
16	15.17988176840517	27.790834399146565	31.96504034373213	25.06424348871613
17	16.001343808754793	27.97811300960116	30.012391920644554	26.008151260999483
18	16.635232543747456	25.79619195247161	33.69924617043974	23.86932933334119
19	17.446527609965404	26.558272969994167	31.56830733141978	24.42689208862065
20	20.005510794674276	28.347660417170108	32.29885599438901	19.347972793766612
21	21.18583755238202	29.687785485686668	30.58513146339595	18.541245498535368
22	19.150085166826784	26.96097355407946	28.888572910465793	25.000368368627964
23	18.390067013620797	27.626836685979008	28.22160467268237	25.761491627717824
24	20.262042707185252	25.755745077121656	29.044761208720608	24.93745100697248
25	17.19176386686863	29.517304484667022	26.397958943106204	26.892972705358144
26	19.375011051058838	28.484693546771027	28.076320085815155	24.063975316354977
27	19.595074469401826	29.207653816004292	28.220425893072903	22.976845821520982
28	15.990882139720746	27.503654216789357	29.923836102483097	26.581627541006796
29	19.746621322944353	26.634083233628225	28.998567782774494	24.62072766065293
30	22.919085620656933	29.14178950532514	26.84928418608215	21.08984068793578
31	23.092881939328215	28.807384464863528	23.754766690045795	24.344966905762462
32	21.355213447517784	31.542079485109063	25.026890909841043	22.075816157532106
33	21.49187820849075	29.796896273288265	24.703905296846177	24.007320221374812
34	22.487283914962838	25.571413415690735	27.28366447217196	24.657638197174464
35	20.887827332362804	27.07649395580755	27.20984339912888	24.82583531270076
36	23.616775802012178	29.05905391148544	25.34118302321606	21.98298726328632
37	18.269094756198907	29.766174329716442	29.099279765658615	22.865451148426036
38	18.163078265072173	30.585794526926275	26.641229585010638	24.609897622990918
39	23.097523384040503	28.306034762210686	28.0477346802855	20.548707173463313
40	26.399137722715672	23.1129948664148	29.13810581904554	21.349761591823984
41	21.279034815255766	26.365689851296953	27.905470716167553	24.44980461727973
42	20.163025219989745	26.387791968974522	29.122339641768875	24.32684316926686
43	21.86901400979566	27.199823772448383	27.24130207995662	23.689860137799336
44	20.63534747475938	24.95299616307237	27.007977391007092	27.403678971161156
45	22.61024536297571	23.041605026316255	25.716992697460316	28.631156913247715
46	24.81257404209422	24.922126872049365	28.113598990964654	22.15170009489176
47	18.49740963180819	25.455893013962644	32.31093848538608	23.73575886884309
48	19.063886907884267	25.431801705694095	29.999277997489198	25.50503338893244
49	22.08966681794338	21.94533998950886	32.4780304950285	23.48696269751926
50	23.032911526696413	21.99661690252082	28.33086280773515	26.63960876304762
51	20.081836774387476	21.178985895901974	26.718365975705353	32.0208113540052
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	301.0
1	466.5
2	632.0
3	1299.0
4	1966.0
5	1426.5
6	887.0
7	1130.0
8	1373.0
9	1939.0
10	2505.0
11	3099.5
12	3694.0
13	3965.0
14	4236.0
15	4120.5
16	4005.0
17	3874.5
18	3744.0
19	3976.5
20	4209.0
21	4468.0
22	4727.0
23	5662.5
24	6598.0
25	7427.0
26	9856.0
27	11456.0
28	13987.0
29	16518.0
30	18576.0
31	20634.0
32	24267.5
33	27901.0
34	31304.5
35	34708.0
36	37137.0
37	39566.0
38	45461.5
39	51357.0
40	59654.0
41	67951.0
42	79109.0
43	90267.0
44	105423.0
45	120579.0
46	155523.5
47	190468.0
48	198576.5
49	206685.0
50	191603.5
51	176522.0
52	134954.5
53	93387.0
54	74597.0
55	55807.0
56	46665.0
57	37523.0
58	30836.0
59	24149.0
60	20482.0
61	16815.0
62	13719.0
63	10623.0
64	9171.5
65	7720.0
66	5543.5
67	3367.0
68	2996.0
69	2625.0
70	2027.0
71	1429.0
72	1101.0
73	773.0
74	733.0
75	535.0
76	377.0
77	282.5
78	188.0
79	124.5
80	61.0
81	42.0
82	23.0
83	16.5
84	10.0
85	14.5
86	19.0
87	10.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1357336.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.46278736701251
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.839415976091004	16.893688906095576
2	13.64590187263392	7.222171993732583
3	6.491440555293989	5.153448333610393
4	3.7146522909532265	3.932002148715244
5	2.4565809050265184	3.2503989069789947
6	1.6543845780868955	2.626777638790693
7	1.162833370450617	2.1540268557850815
8	0.8970527138157313	1.8990812178165772
9	0.6563017436383713	1.5630816141451596
>10	4.652237772468577	26.595960573471377
>50	0.6739395970793535	11.507161432369768
>100	0.13371823878420686	6.720297762751739
>500	0.013148027621318031	2.346478054783873
>1k	0.006434141176389675	3.035682417658525
>5k	0.0013987263426934076	2.996521396395293
>10k+	5.59490537077363E-4	2.103220746899221
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	16568	1.2206262856065115	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGCT	11843	0.8725179321848091	TruSeq Adapter, Index 21 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGC	9842	0.7250968072754277	No Hit
TCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	9351	0.6889230080098074	TruSeq Adapter, Index 15 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	8284	0.6103131428032558	TruSeq Adapter, Index 15 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTC	7038	0.5185156807157549	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCG	5963	0.4393164257044681	No Hit
TCCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTG	3604	0.26552010703318857	TruSeq Adapter, Index 21 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACAGGTAT	3562	0.2624258105583289	No Hit
CGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTG	2835	0.20886501205302152	TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCC	2226	0.16399771316755762	No Hit
TTCCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCT	2209	0.1627452598324954	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTC	2072	0.15265195942640583	No Hit
TGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTG	1980	0.14587397667195154	TruSeq Adapter, Index 21 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTG	1804	0.13290740096777806	No Hit
CCTGACTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	1799	0.1325390323398186	TruSeq Adapter, Index 15 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCT	1766	0.13010779939528608	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTC	1711	0.12605574448773185	No Hit
TTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTG	1644	0.12111960487307491	TruSeq Adapter, Index 21 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCT	1553	0.11441529584421248	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACAGGTATCGTA	1435	0.1057217962243689	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9210689173498677	0.0
2	0.0	0.0	0.0	3.640145107769926	0.0
3	7.367372559189471E-5	0.0	0.0	4.892966811460095	0.0
4	7.367372559189471E-5	0.0	0.0	6.306691931842963	0.0
5	7.367372559189471E-5	0.0	0.0	8.998582517519612	0.0
6	7.367372559189471E-5	0.0	0.0	10.384532643354335	0.0
7	7.367372559189471E-5	0.0	0.0	11.761420900941255	0.0
8	7.367372559189471E-5	0.0	0.0	13.901937324288165	0.0
9	7.367372559189471E-5	0.0	0.0	14.673522252412077	0.0
10	7.367372559189471E-5	0.0	0.0	16.916150459429353	0.0
11	7.367372559189471E-5	0.0	0.0	20.370343083805338	0.0
12	7.367372559189471E-5	0.0	0.0	23.75027259278469	0.0
13	7.367372559189471E-5	0.0	0.0	24.62978952890073	0.0
14	7.367372559189471E-5	0.0	0.0	24.919695639104834	0.0
15	7.367372559189471E-5	0.0	0.0	25.424508006860496	0.0
16	7.367372559189471E-5	0.0	0.0	26.524014687593933	0.0
17	7.367372559189471E-5	0.0	0.0	28.10615794467987	0.0
18	0.0013261270606541049	0.0	0.0	29.80094832819582	0.0
19	0.0013261270606541049	0.0	0.0	31.30698662674533	0.0
20	0.0013261270606541049	0.0	0.0	32.52945475549164	0.0
21	0.0013261270606541049	0.0	0.0	34.258356073956634	0.0
22	0.0013261270606541049	0.0	0.0	35.94990481354654	0.0
23	0.0013261270606541049	0.0	0.0	37.52357559218941	0.0
24	0.0013261270606541049	0.0	0.0	38.842556301461094	0.0
25	0.0013261270606541049	0.0	0.0	39.95245097750299	0.0
26	0.0013261270606541049	0.0	0.0	40.93179581179605	0.0
27	0.0013261270606541049	0.0	0.0	41.83555140363182	0.0
28	0.0013261270606541049	0.0	0.0	42.7401174064491	0.0
29	0.0013261270606541049	0.0	0.0	43.66442796772501	0.0
30	0.0013261270606541049	0.0	0.0	44.73866456058043	0.0
31	0.0013261270606541049	0.0	0.0	45.675794350109335	0.0
32	0.0013261270606541049	0.0	0.0	46.57564523448873	0.0
33	0.0013261270606541049	0.0	0.0	47.42878697684287	0.0
34	0.0013261270606541049	0.0	0.0	48.2547431144536	0.0
35	0.0013261270606541049	0.0	0.0	49.15341521922354	0.0
36	0.0013261270606541049	0.0	0.0	49.9645629379903	0.0
37	0.0013261270606541049	0.0	0.0	50.80554851562178	0.0
38	0.0013261270606541049	0.0	0.0	51.626052797538705	0.0
39	0.0013261270606541049	0.0	0.0	52.4096465429341	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCTA	25	3.8911792E-5	45.000004	12
ACTAGTG	25	3.8911792E-5	45.000004	45
GCGATCG	35	1.2121382E-7	45.000004	9
ACGTAAC	50	2.1827873E-11	45.000004	15
CGTCCAA	25	3.8911792E-5	45.000004	11
GCGAGAC	20	7.0337387E-4	45.0	21
AGCGTCA	20	7.0337387E-4	45.0	11
CTGTACG	45	3.8562575E-10	45.0	1
AGCCCGC	20	7.0337387E-4	45.0	32
CAGTTCG	20	7.0337387E-4	45.0	1
CATTGCG	20	7.0337387E-4	45.0	1
ACCGAGC	20	7.0337387E-4	45.0	12
CGACATA	20	7.0337387E-4	45.0	10
CCATGCG	20	7.0337387E-4	45.0	21
CGTTACC	20	7.0337387E-4	45.0	17
CGCTAAA	20	7.0337387E-4	45.0	19
GTCACGG	20	7.0337387E-4	45.0	2
TTGCGGA	55	1.8189894E-12	45.0	3
AGCGATA	20	7.0337387E-4	45.0	24
ACGTCGG	20	7.0337387E-4	45.0	24
>>END_MODULE